CAZyme Information: MGYG000000612_00971

Basic Information

• Lineage: Bacteria; Firmicutes_C; Negativicutes; Veillonellales; Megasphaeraceae; Megasphaera
• CAZyme ID: MGYG000000612_00971
• CAZy Family: GT83
• CAZyme Description: Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
511		58355.72	9.2819

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000612	1970930	MAG	Madagascar	Africa

• Gene Location: Start: 7880; End: 9415 Strand: +

Full Sequence      

MYEFRAKEDAALSIWQYLWQKIMASPVVILLVLAAILCFVANHLLVVTDPVESNYVLTAK
EMLASGDYFSPRIFGNYWYDKPIFFYWELIAAFKLFGIGNFSARFFPALFGMAGILLAYG
FTARLYDKKTGFLTGLILLTTVEYFYLSKAIITDMTLFVFYSACLMAFYIAYSEGKPRWY
YLAYAFAGLAVLTKGPIGFFQPGLIILLFLLWRRDLKALLHIKLFSGLVLFLAITLIWYG
PMYAMHGSDFVSQFIGVHNVLRATVSEHPQFNVWYYYSAIFLAGFIPWIFTLPLAWRDYG
LTRKIAAAWREKRLHLPAFSMKTQFLIVWAATIFVTYQLMATKYMTYTFPYMIPLAIGFA
SYLKKYDRLVLNMAGIVLAIYVAATCTVIVPQCQQASAYNAAQVVSGLADNETTVVTYGG
RYPVSLTYYSGHTAYRLAEAKDIAHMLPGGISWNAKNVMPFMAIEDLPDHPKVLAVVRDR
DEAHFLQEVPGNWKWRTQKGIWAIYQKEADL

Enzyme Prediction     

No EC number prediction in MGYG000000612_00971.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT83	27	417	1e-90	0.6981481481481482

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG1807	ArnT	2.49e-45	19	360	2	333	4-amino-4-deoxy-L-arabinose transferase or related glycosyltransferase of PMT family [Cell wall/membrane/envelope biogenesis].
PRK13279	arnT	1.94e-25	23	365	2	335	lipid IV(A) 4-amino-4-deoxy-L-arabinosyltransferase.
pfam13231	PMT_2	1.27e-20	80	238	1	160	Dolichyl-phosphate-mannose-protein mannosyltransferase. This family contains members that are not captured by pfam02366.
pfam02366	PMT	8.41e-13	52	246	32	240	Dolichyl-phosphate-mannose-protein mannosyltransferase. This is a family of Dolichyl-phosphate-mannose-protein mannosyltransferase proteins EC:2.4.1.109. These proteins are responsible for O-linked glycosylation of proteins, they catalyze the reaction:- Dolichyl phosphate D-mannose + protein <=> dolichyl phosphate + O-D-mannosyl-protein. Also in this family is Drosophila rotated abdomen protein which is a putative mannosyltransferase. This family appears to be distantly related to pfam02516 (A Bateman pers. obs.). This family also contains sequences from ArnTs (4-amino-4-deoxy-L-arabinose lipid A transferase). They catalyze the addition of 4-amino-4-deoxy-l-arabinose (l-Ara4N) to the lipid A moiety of the lipopolysaccharide. This is a critical modification enabling bacteria (e.g. Escherichia coli and Salmonella typhimurium) to resist killing by antimicrobial peptides such as polymyxins. Members such as undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase are predicted to have 12 trans-membrane regions. The N-terminal portion of these proteins is hypothesized to have a conserved glycosylation activity which is shared between distantly related oligosaccharyltransferases ArnT and PglB families.
COG4745	COG4745	2.34e-07	75	194	56	177	Predicted membrane-bound mannosyltransferase [General function prediction only].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AVO27725.1	0.0	1	511	1	512
AVO74995.1	0.0	1	511	1	512
CCC74317.1	0.0	1	511	1	512
ALG42265.1	0.0	11	511	2	502
AXL22207.1	1.46e-207	9	508	13	521

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5EZM_A	1.36e-25	27	350	34	351	CrystalStructure of ArnT from Cupriavidus metallidurans in the apo state [Cupriavidus metallidurans CH34],5F15_A Crystal Structure of ArnT from Cupriavidus metallidurans bound to Undecaprenyl phosphate [Cupriavidus metallidurans CH34]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O67270	2.09e-24	50	365	28	323	Uncharacterized protein aq_1220 OS=Aquifex aeolicus (strain VF5) OX=224324 GN=aq_1220 PE=3 SV=1
A4SQX1	4.09e-21	49	361	27	328	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Aeromonas salmonicida (strain A449) OX=382245 GN=arnT PE=3 SV=1
Q4K884	4.62e-21	50	361	63	362	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase 2 OS=Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5) OX=220664 GN=arnT2 PE=3 SV=1
A0KGY4	1.74e-20	49	361	27	328	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / BCRC 13018 / CCUG 14551 / JCM 1027 / KCTC 2358 / NCIMB 9240 / NCTC 8049) OX=380703 GN=arnT PE=3 SV=1
C5BDQ8	5.59e-20	49	365	29	334	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Edwardsiella ictaluri (strain 93-146) OX=634503 GN=arnT PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000015	0.000007	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
25	47
83	102
109	126
130	152
157	174
189	211
218	240
274	296
316	335
345	362
369	391