CAZyme Information: MGYG000000576_01484

Basic Information

• Lineage: Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae; Streptococcus
• CAZyme ID: MGYG000000576_01484
• CAZy Family: GT4
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
667		76663.15	6.6915

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000576	1830013	MAG	Madagascar	Africa

• Gene Location: Start: 599; End: 2602 Strand: +

Full Sequence      

MNRIKRILSRLKQIVLHVPLYAKKLKDKIIRFFLLRYRQLTRKNKYGNSPRRVLIYNIFE
SGESLQKHKLLFLKALSEIADDVHIIVNGSLSESDVALLKPYGKVTLRENKGYDVAAFRQ
GILELGQSGLANYDQLLLVNDTNVGPFSDLESLFRRMPSDKLDFWGISLGEIQLDFTGLN
PYGYIPEHVQTYFLVIEKSLLSSPSFYRYWERLDDTDSRQKAIGKHETVFTKYFSNLGFV
YDAVIRDTTDSAMYIHPLRVLKEGAPLVKFSAFSNYDDDQFIWTGLERKSEVPELINYIS
TETDYPLEIINDIVEQFKKKSQNQYVLIIDGVENIIPQCTRYRVLNKAEQLERLGYQVKI
VNHSQLELLDGKYASHIIIYRAPDSPLLTHFCQLAKLTGKTVYYDIDDLVFDTKYTDQLE
YTQNLSTIEKGNYDAGVHGYGRMLAMCDVAITSTKHLALELAKYKEKVILNRNVLSKELL
NISLKTLHLVSDNQKIKLAYFSGSITHNENFELIKPAIINLLKKYKHLELHLAGHLDVPA
DIVCFGDQIVTHPYVDWKELPQLISSIDINLAPLVDVTFNRAKSEIKWLEAAIVKVPTVA
SKVGSFEEMILDGQTGLLADPSQWEEKLEFLILDATARERISDNSYQFVKNHCTTQSEIK
DFLLLEE

Enzyme Prediction     

No EC number prediction in MGYG000000576_01484.

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam05045	RgpF	1.21e-52	85	327	3	254	Rhamnan synthesis protein F. This family consists of a group of proteins which are related to the Streptococcus rhamnose-glucose polysaccharide assembly protein (RgpF). Rhamnan backbones are found in several O polysaccharides of phytopathogenic bacteria and are regarded as pathogenic factors.
COG3754	RgpF	1.49e-35	51	312	2	261	Lipopolysaccharide biosynthesis protein [Cell wall/membrane/envelope biogenesis].
cd03794	GT4_WbuB-like	3.47e-32	317	663	41	391	Escherichia coli WbuB and similar proteins. This family is most closely related to the GT1 family of glycosyltransferases. WbuB in E. coli is involved in the biosynthesis of the O26 O-antigen. It has been proposed to function as an N-acetyl-L-fucosamine (L-FucNAc) transferase.
cd03801	GT4_PimA-like	3.43e-18	348	663	24	362	phosphatidyl-myo-inositol mannosyltransferase. This family is most closely related to the GT4 family of glycosyltransferases and named after PimA in Propionibacterium freudenreichii, which is involved in the biosynthesis of phosphatidyl-myo-inositol mannosides (PIM) which are early precursors in the biosynthesis of lipomannans (LM) and lipoarabinomannans (LAM), and catalyzes the addition of a mannosyl residue from GDP-D-mannose (GDP-Man) to the position 2 of the carrier lipid phosphatidyl-myo-inositol (PI) to generate a phosphatidyl-myo-inositol bearing an alpha-1,2-linked mannose residue (PIM1). Glycosyltransferases catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. The acceptor molecule can be a lipid, a protein, a heterocyclic compound, or another carbohydrate residue. This group of glycosyltransferases is most closely related to the previously defined glycosyltransferase family 1 (GT1). The members of this family may transfer UDP, ADP, GDP, or CMP linked sugars. The diverse enzymatic activities among members of this family reflect a wide range of biological functions. The protein structure available for this family has the GTB topology, one of the two protein topologies observed for nucleotide-sugar-dependent glycosyltransferases. GTB proteins have distinct N- and C- terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. The members of this family are found mainly in certain bacteria and archaea.
pfam00534	Glycos_transf_1	2.60e-13	497	648	3	158	Glycosyl transferases group 1. Mutations in this domain of PIGA lead to disease (Paroxysmal Nocturnal haemoglobinuria). Members of this family transfer activated sugars to a variety of substrates, including glycogen, Fructose-6-phosphate and lipopolysaccharides. Members of this family transfer UDP, ADP, GDP or CMP linked sugars. The eukaryotic glycogen synthases may be distant members of this family.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CCF02594.1	0.0	3	663	14	674
VEE22879.1	0.0	3	663	12	672
QGX44978.1	0.0	24	663	39	674
QGX47164.1	0.0	24	663	39	674
SQF42567.1	0.0	3	663	12	672

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5I45_A	3.94e-06	512	658	46	201	1.35Angstrom Crystal Structure of C-terminal Domain of Glycosyl Transferase Group 1 Family Protein (LpcC) from Francisella tularensis. [Francisella tularensis subsp. tularensis SCHU S4]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000074	0.000001	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000576_01484.