CAZyme Information: MGYG000000515_00631

Basic Information

• Species: UBA2882 sp900549665
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; UBA2882; UBA2882 sp900549665
• CAZyme ID: MGYG000000515_00631
• CAZy Family: CBM48
• CAZyme Description: 1,4-alpha-glucan branching enzyme GlgB

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
822	MGYG000000515_2|CGC2	94312.8	5.4747

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000515	2818145	MAG	Fiji	Oceania

• Gene Location: Start: 268999; End: 271467 Strand: +

Full Sequence      

MTNKLYKLMDWAAIEGITYSEEDQPQNILGVHPVSGGNLVQLFYPQAVEAVLHTKNKEYA
MELADEEGFFAVLIPKTVSLDKYSFSVRMEDGTTDTFYDPYAYEPVITKKDAEKFNAGIH
YEIYHLLGAHPYVIDGVHGVLFAVWAPNAMRVSVVGDFNRWDGRMHQMRRLWDSGIFELF
IPKANIGDNYKFEIKAKGGLTFLKADPYGIYSQKRPDTASIVYDLTGFSWEDASWIENRK
KTDMHKNPMSVLEIHLGSFAKPDDGRDFYNYRELAPKVAEYAKKTGYTHVELMPVMEHPL
DASWGYQVIGYYAPTSRYGTPQDFQYFINYLHKEGLGVILDWVPAHFPRDVHGLCGFDGT
SLYEPADKRKAEHPHWGTLIFDYGRPEVKNYLIANALYWFKEYHADGIRMDAVASMLYLD
YGRNDGDWVPNMYGGKENLEAVEFLKHLNSIVKKTDSSVMMIAEESTAWPNVTAPVKKDG
LGFDYKWNMGWMNDFLDYMKCDPLFRAGRHGELTFSMVYAYSEHFILSLSHDEVVHGKSA
MVGKMPGDIPEKFANLRAAYGYMMMHPGKKLLFMGQDFGEFDEWNEERSIEWDLLKYDDH
KNMHQYMTALNELYKSHPALYTMDENPDGFEWINNISGNENTLVFIRKTGDAKESLVVVC
NFSGVDRKGYKIGVPYAGKYKEIFNSDLKKYGGNGFSNTKALSSDLDECDEREESIRITS
PAFSVVVFSYTPFSEKEKAAIEEKRAIARQRREESRKLQELIAQEDELKESAITARAVAQ
EAELVAKDALKKSMDAAQRAQDLEKKAKDIENKRKKFEEEMI

Enzyme Prediction     

EC	2.4.1.18

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH13	280	580	6.7e-155	0.9966777408637874
CBM48	127	211	3.8e-17	0.8552631578947368

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK05402	PRK05402	0.0	25	728	17	721	1,4-alpha-glucan branching protein GlgB.
PRK14705	PRK14705	0.0	9	728	503	1220	glycogen branching enzyme; Provisional
PRK12313	PRK12313	0.0	105	732	5	629	1,4-alpha-glucan branching protein GlgB.
PRK12568	PRK12568	0.0	28	726	29	724	glycogen branching enzyme; Provisional
COG0296	GlgB	0.0	102	730	1	628	1,4-alpha-glucan branching enzyme [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AWY97839.1	0.0	1	731	1	733
CBK76766.1	0.0	1	736	1	748
QNM06418.1	0.0	1	745	1	756
CBL36572.1	0.0	1	736	1	748
QNM09059.1	0.0	1	749	1	752

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5GQW_A	4.26e-251	14	743	30	786	Crystalstructure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQX_A Crystal structure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR5_A	6.03e-251	14	743	30	786	Crystalstructure of branching enzyme W610A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQZ_A	8.53e-251	14	743	30	786	Crystalstructure of branching enzyme Y500A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GR1_A	8.53e-251	14	743	30	786	Crystalstructure of branching enzyme Y500A/D501A mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142],5GR6_A Crystal structure of branching enzyme Y500A/D501A double mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQU_A	1.21e-250	14	743	30	786	Crystalstructure of branching enzyme from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQV_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltohexaose [Crocosphaera subtropica ATCC 51142],5GQY_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q8DLB8	7.60e-263	14	745	8	761	1,4-alpha-glucan branching enzyme GlgB OS=Thermosynechococcus vestitus (strain IAM M-273 / NIES-2133 / BP-1) OX=197221 GN=glgB PE=3 SV=1
Q3M473	2.24e-256	14	740	11	759	1,4-alpha-glucan branching enzyme GlgB OS=Trichormus variabilis (strain ATCC 29413 / PCC 7937) OX=240292 GN=glgB PE=3 SV=1
P16954	3.60e-255	14	727	17	756	1,4-alpha-glucan branching enzyme GlgB OS=Synechococcus elongatus (strain PCC 7942 / FACHB-805) OX=1140 GN=glgB PE=1 SV=2
Q5N4W5	3.60e-255	14	727	17	756	1,4-alpha-glucan branching enzyme GlgB OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) OX=269084 GN=glgB PE=3 SV=1
Q8YYX9	8.30e-254	14	740	11	759	1,4-alpha-glucan branching enzyme GlgB OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=glgB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000035	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000515_00631.