CAZyme Information: MGYG000000456_00409

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Acutalibacteraceae
• CAZyme ID: MGYG000000456_00409
• CAZy Family: GH36
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1325		147147.49	4.3112

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000456	2140419	MAG	Sweden	Europe

• Gene Location: Start: 2305; End: 6282 Strand: -

Full Sequence      

MNCKKWWRVLSAAMSAAVCMAGIPFSSSAAQTTALNEAGDVVVRSPSSVDLNQLIYLDDH
LTVSGEAKADSVRIYLEDELIGTAPVTGTKFTCEVEGLSQAEQTAALRVVGVVDGQEMTD
KGLPLSVQVGGDVVLASDAYVSRVAALGDSFRRNSSANGGLAIGGGSYTSEKGFEIHPKN
GKEASAYAEIDIPVEGRGYTYFQSYVGRNAFQGSGECDDVECIVLADGVEVARSGQLAGQ
TVKLLCATIPADAKLITLRTTNYDGIYNFGSTDWLNPTLALSRECIYRDGVKMYDESQES
QVIDLNGANLQFKALQDFNALGLELESVPEDQEITVKLYRWASSYYRTINDMPVVTAITR
ANDNMAFVDFSGFVAAGEYLAVIEGAGTIRAYASDKFYRYDKEQKSQALRAQLVFTEETE
RPFGIAVPSFGSTALDVMAPTKEERERIEEEYRGFLSDNLKNFPMSFCIDGKEYFGFGVD
FTLESQKTETQNDIETTVTQLKHTSGLVFEVTSVYYPNYNAYDWVVYISNNTGTDSPIIS
GINAADLTLRGKDPYLFGNYGDSQQYKPYTVDVDGTYTTSSKGGRSTQANSSYFNLEYGS
KVVLYAIGWPGQWSMTLDNSENPGITRLVAGQESFSSYLKNGETIRTPLMAFVHYDGQDQ
DRATNLWRHWMIDCNMHRVTEDASGEGELKLMDPMLCASSSFQYGVMTMATDQNQIDAIR
TYRENGINLNYWWMDAGWYYNIDDRGNYVSLPANGWPGTGVWKVDDTKFPSKFLDISNFA
KSYGMKTLLWFEPERSFAPVNGLKDNGETIRREWLISGVLVNMGLSDAVDWFSDRIISIM
REGGISLYREDFNIEPLSYWKTGDRSQGRNRDGITENLYVQGHLKLWDRILEAFPDAVID
SCASGGNRNDLETMRRAVPLHKTDYGYGDRPAQQGIATEMSRWIPYFGTKANGEGGSDNN
NKTANRYSLRTAMASSMVLDYITDSPVDWDIVQDITQEHAELAAFNYGDYYVLSNWSRSE
SDWAAWEYYCPEQGRGYAMVYRRNRAATSQKILLKGLETNATYKIWFEDANKPAYYTGQE
LMLDGLEVSLPADNTSDIIHIAKKELNIADRRLTAKVTQISENGQYQGALRPYDGYYRVD
IRFNMALRDTVLENRAGIVETGVQGDYTDTILLNGTSIGEILSEDADGVYLDYDVSANIL
RVYAKEAYLSWNRTNQIEVSETLCSDGGAVMDGKTTFYYSPMGDVWSQETVPSDSDSNRM
GDVNGDGNVDASDALLVLQHSVNLTTLNERQQAAANVNADDSIDASDALLILQRSVGLID
GFPAE

Enzyme Prediction     

No EC number prediction in MGYG000000456_00409.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH36	535	1087	3.8e-40	0.7805232558139535

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd14791	GH36	1.23e-21	726	917	30	212	glycosyl hydrolase family 36 (GH36). GH36 enzymes occur in prokaryotes, eukaryotes, and archaea with a wide range of hydrolytic activities, including alpha-galactosidase, alpha-N-acetylgalactosaminidase, stachyose synthase, and raffinose synthase. All GH36 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein. GH36 members are retaining enzymes that cleave their substrates via an acid/base-catalyzed, double-displacement mechanism involving a covalent glycosyl-enzyme intermediate. Two aspartic acid residues have been identified as the catalytic nucleophile and the acid/base, respectively.
cd14256	Dockerin_I	4.02e-14	1261	1313	2	54	Type I dockerin repeat domain. Bacterial cohesin domains bind to a complementary protein domain named dockerin, and this interaction is required for the formation of the cellulosome, a cellulose-degrading complex. The cellulosome consists of scaffoldin, a noncatalytic scaffolding polypeptide, that comprises repeating cohesion modules and a single carbohydrate-binding module (CBM). Specific calcium-dependent interactions between cohesins and dockerins appear to be essential for cellulosome assembly. This subfamily represents type I dockerins, which are responsible for anchoring a variety of enzymatic domains to the complex.
pfam02065	Melibiase	1.13e-12	734	911	77	247	Melibiase. Glycoside hydrolase families GH27, GH31 and GH36 form the glycoside hydrolase clan GH-D. Glycoside hydrolase family 36 can be split into 11 families, GH36A to GH36K. This family includes enzymes from GH36A-B and GH36D-K and from GH27.
COG3345	GalA	9.87e-12	734	1108	328	687	Alpha-galactosidase [Carbohydrate transport and metabolism].
pfam00404	Dockerin_1	3.38e-10	1261	1314	1	54	Dockerin type I repeat. The dockerin repeat is the binding partner of the cohesin domain pfam00963. The cohesin-dockerin interaction is the crucial interaction for complex formation in the cellulosome. The dockerin repeats, each bearing homology to the EF-hand calcium-binding loop bind calcium.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BBO31825.1	1.40e-106	480	1104	73	685
BBO33728.1	2.02e-101	459	1103	46	686
QOY88337.1	1.44e-95	464	1099	175	786
BBO33698.1	3.78e-94	505	1105	94	689
QGQ94286.1	4.63e-88	435	1085	5	650

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3MI6_A	9.60e-11	734	1081	366	707	ChainA, Alpha-galactosidase [Levilactobacillus brevis ATCC 367],3MI6_B Chain B, Alpha-galactosidase [Levilactobacillus brevis ATCC 367],3MI6_C Chain C, Alpha-galactosidase [Levilactobacillus brevis ATCC 367],3MI6_D Chain D, Alpha-galactosidase [Levilactobacillus brevis ATCC 367]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P16551	3.88e-10	734	916	328	497	Alpha-galactosidase OS=Escherichia coli OX=562 GN=rafA PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000738	0.994502	0.003957	0.000321	0.000228	0.000203

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000456_00409.