CAZyme Information: MGYG000000323_01166

Basic Information

• Species: Enterococcus_D sp002850555
• Lineage: Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Enterococcus_D; Enterococcus_D sp002850555
• CAZyme ID: MGYG000000323_01166
• CAZy Family: GH94
• CAZyme Description: 1,2-beta-oligoglucan phosphorylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1096	MGYG000000323_3|CGC4	123608.58	4.85

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000323	3361404	MAG	Sweden	Europe

• Gene Location: Start: 121828; End: 125118 Strand: -

Full Sequence      

MTKKIVMNNENTTVTFLPNGDLYEIQSHGVMINQLNGNALDGSLNQIYLRLREAGGLSFI
PLIGSNADSAFVYSDKQLTWTGTYQSINYQVDFQLAQDCWFWRVQLSGSGEAELVYGQDL
GNAAKGAVQSNEAYVSQYIDHHVSHDKDHIVLSSRQNQPQNGQFPLVEQGSFQALKGFST
DGYQFFGRSYKATNQPAALSQETLANEVYQYEFAYTALQTQWLSVSETPTEIVFYAAVKA
NQATAVNEPQFALEPLKETYQALSFDSLQATAQPRKNFGQPLTGLTFTSEEINERFPQQE
AVEIVDEQLYSFFTPDYHHVVLKEKEAQMERSHGHILMSGQDLIVDQPILSTTVYMTGMF
NSQIVLGNTNMNKLLSNSRNSLNLFKRSGQRIYLKDGDQWRILTMPSAFEMGLNSATWYY
KTADDVIQVTTFTKANGRTIATTITSEQGRAYTWAITNQFVMGIDEAAPTATISQDQQLL
TIKGTADSPIAETYPELTYYLHAAQPFELTDETIFNVAADDSTTVLTFAEQATVSFVIQG
TLTGEPFVSETLDRQQEDTAYTAFVDDLLNHFELKHSQADVASFNHLARWYTHNMLVHYL
SPHGLEQYGGAAWGTRDVSQGPTEYFLALNRPEMVASIIEHLFENQFADDGNWPQWFMFD
RYEKQKADESHGDVIVWPMKVVSDYLEKTKDFAILEKELPYTDRATFLKTKTKASLFDHL
KKEVAYIEANFLEGTYLSCYGDGDWDDTLQPNNSKLKKQMASSWTVALTYEVLKKLASQL
QSVDPEYAKHLTELSAGIKHDFEKYMLADGTLPGFVYMEDSEHVELMVHPTDKKTGIQYR
LLPMQQSMIGELLSPEQADHHVAIIKEHLQFPDGVRLMNRPATYAGGVSTNFKRAEQAAN
FGREIGLQYVHAHIRFTEAMAKLGREEETWQALGVINPIQIAQRVENAEIRQANAYFSSS
DGDFKTRVEAQENFGKLKEAAVGVKGGWRIYSSGPGIYMNQLISNVLGIRTFVDHVELDP
VLPAELAGLTLTYRLFDRPVEIVYHSASTPKVLINGEEISTEFAENRYRQGAFVLKKAAL
CAKLNENHTNTIDIYR

Enzyme Prediction     

EC	2.4.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH94	96	1064	8.7e-188	0.9536679536679536

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3459	COG3459	0.0	16	1062	22	1036	Cellobiose phosphorylase [Carbohydrate transport and metabolism].
pfam17167	Glyco_hydro_36	7.68e-12	631	1010	67	425	Glycosyl hydrolase 36 superfamily, catalytic domain. This is the catalytic region of the superfamily of enzymes referred to as GH36. UniProtKB:Q76IQ9 is a chitobiose phosphorylase that catalyzes the reversible phosphorolysis of chitobiose into alpha-GlcNAc-1-phosphate and GlcNAc with inversion of the anomeric configuration. The full-length enzyme comprises a beta sandwich domain and an (alpha/alpha)(6) barrel domain. The alpha-helical barrel component of the domain, this family, is the catalytic region.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AYJ43955.1	0.0	1	1096	1	1096
ATF72350.1	0.0	1	1096	1	1096
AUJ84246.1	0.0	1	1096	1	1096
EEV39210.2	0.0	1	1096	1	1096
AMG49510.1	0.0	1	1096	1	1096

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5H41_A	5.80e-278	5	1088	11	1107	CrystalStructure of 1,2-beta-oligoglucan phosphorylase from Lachnoclostridium phytofermentans in complex with sophorose, isofagomine, sulfate ion [Lachnoclostridium phytofermentans ISDg],5H41_B Crystal Structure of 1,2-beta-oligoglucan phosphorylase from Lachnoclostridium phytofermentans in complex with sophorose, isofagomine, sulfate ion [Lachnoclostridium phytofermentans ISDg],5H42_A Crystal Structure of 1,2-beta-oligoglucan phosphorylase from Lachnoclostridium phytofermentans in complex with alpha-d-glucose-1-phosphate [Lachnoclostridium phytofermentans ISDg],5H42_B Crystal Structure of 1,2-beta-oligoglucan phosphorylase from Lachnoclostridium phytofermentans in complex with alpha-d-glucose-1-phosphate [Lachnoclostridium phytofermentans ISDg]
5H3Z_A	1.46e-269	5	1088	11	1107	CrystalStructure of 1,2-beta-oligoglucan phosphorylase from Lachnoclostridium phytofermentans [Lachnoclostridium phytofermentans ISDg],5H3Z_B Crystal Structure of 1,2-beta-oligoglucan phosphorylase from Lachnoclostridium phytofermentans [Lachnoclostridium phytofermentans ISDg],5H40_A Crystal Structure of 1,2-beta-oligoglucan phosphorylase from Lachnoclostridium phytofermentans in complex with sophorose [Lachnoclostridium phytofermentans ISDg],5H40_B Crystal Structure of 1,2-beta-oligoglucan phosphorylase from Lachnoclostridium phytofermentans in complex with sophorose [Lachnoclostridium phytofermentans ISDg]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q92AT0	2.18e-305	13	1086	13	1075	1,2-beta-oligoglucan phosphorylase OS=Listeria innocua serovar 6a (strain ATCC BAA-680 / CLIP 11262) OX=272626 GN=lin1839 PE=1 SV=1
P20471	1.31e-07	583	1035	2360	2796	Cyclic beta-(1,2)-glucan synthase NdvB OS=Rhizobium meliloti (strain 1021) OX=266834 GN=ndvB PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000072	0.000004	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000323_01166.