CAZyme Information: MGYG000000050_00901

Basic Information

• Species: Blautia_A sp900066505
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Blautia_A; Blautia_A sp900066505
• CAZyme ID: MGYG000000050_00901
• CAZy Family: CBM48
• CAZyme Description: 1,4-alpha-glucan branching enzyme GlgB

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
627		72808.49	6.7058

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000050	3884369	Isolate	United Kingdom	Europe

• Gene Location: Start: 120915; End: 122798 Strand: -

Full Sequence      

MGERMQFTELDQYLFGQATHYDLYKKLGAHPVSYGRQKGVYFAVWAPNAQYVSVIGEFND
WDTQANPMEKAGPIGVFQVFVPGAREGQLYKFYIVGMHGEELYKADPYANAAEMRPGTAS
RITDISSYKWNDGVWMKNRPRFNEKKDAMAIYEVHPGSWMKHPSTEDNREGFFNYREFGE
MLAKYVKEMGYTHVELMGIAEHPFDGSWGYQVTGYYAPTSRYGTPQDFKYMVDYLHRQKI
GVILDWVPAHFPKDAHGLANFDGTAVYEHEDPRQGEHPDWGTKIYNYGRPEVKNFLIANA
LFWIEECHVDGLRVDAVASMLYLDYGKSDGQWVANKYGGNENLEAIEFFKHLNTVVLGRN
HGTVMIAEESTAWPKVTGKAEDGGLGFSLKWNMGWMNDFLEYMKLDPYFRKDNHNKMTFA
ITYAYSEKYVLVLSHDEVVHLKCSMLSKMPGELEDKFKNLKAGYTFMMGHPGKKLLFMGQ
DFGQLREWSEERELDWFLLQEKEHRDLQSFVKDLLYIYRKYPAVHATDNDPQGFEWINAD
DAYRSIFSFVRKSPTKRNNLLFICNFTPVARPDYRVGVPRLKQYTQLMDENGRTGKKVFR
AVKQECDNRPYSFAYPLPAYGIAIFQY

Enzyme Prediction     

EC	2.4.1.18

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH13	185	484	2.5e-153	0.9933554817275747
CBM48	26	112	1.6e-20	0.9078947368421053

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK12313	PRK12313	0.0	6	627	6	627	1,4-alpha-glucan branching protein GlgB.
PRK14705	PRK14705	0.0	9	625	605	1220	glycogen branching enzyme; Provisional
COG0296	GlgB	0.0	8	627	7	628	1,4-alpha-glucan branching enzyme [Carbohydrate transport and metabolism].
PRK14706	PRK14706	0.0	27	627	27	621	glycogen branching enzyme; Provisional
PRK05402	PRK05402	0.0	6	626	99	722	1,4-alpha-glucan branching protein GlgB.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QCU01659.1	0.0	1	627	1	628
CBL22762.1	0.0	1	627	1	628
QIB55783.1	0.0	2	627	4	636
QMW76350.1	0.0	2	627	4	636
ASU30768.1	0.0	5	627	7	636

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5GQW_A	1.94e-223	8	626	130	772	Crystalstructure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQX_A Crystal structure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR5_A	2.75e-223	8	626	130	772	Crystalstructure of branching enzyme W610A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQZ_A	3.90e-223	8	626	130	772	Crystalstructure of branching enzyme Y500A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GR2_A	3.90e-223	8	626	130	772	Crystalstructure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GR4_A Crystal structure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GQU_A	5.52e-223	8	626	130	772	Crystalstructure of branching enzyme from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQV_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltohexaose [Crocosphaera subtropica ATCC 51142],5GQY_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P30539	5.90e-289	1	627	1	632	1,4-alpha-glucan branching enzyme GlgB OS=Butyrivibrio fibrisolvens OX=831 GN=glgB PE=1 SV=1
Q7NL20	1.17e-235	8	626	104	727	1,4-alpha-glucan branching enzyme GlgB OS=Gloeobacter violaceus (strain ATCC 29082 / PCC 7421) OX=251221 GN=glgB PE=3 SV=1
Q2JT08	2.13e-232	9	591	119	710	1,4-alpha-glucan branching enzyme GlgB OS=Synechococcus sp. (strain JA-3-3Ab) OX=321327 GN=glgB PE=3 SV=1
Q11EX1	1.19e-231	7	624	113	733	1,4-alpha-glucan branching enzyme GlgB OS=Chelativorans sp. (strain BNC1) OX=266779 GN=glgB PE=3 SV=1
Q1AZ86	1.39e-231	8	618	100	710	1,4-alpha-glucan branching enzyme GlgB OS=Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129 / PRD-1) OX=266117 GN=glgB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000073	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000050_00901.