Browse dbCAN-PUL Entries

PULID Characterization Method(s) Substrate Organism Publication Publish Date Type Num Genes Num CAZymes CazyFamily
PUL0115 expression of recombinant proteins, RNA-seq, differential gene expression host glycan Bacteroides thetaiotaomicron <a href=https://pubmed.ncbi.nlm.nih.gov/31160824/>31160824</a>
Complex N-glycan breakdown by gut Bacteroides involves an extensive enzymatic apparatus encoded by multiple co-regulated genetic loci. Nat Microbiol. 2019 Sep;4(9):1571-1581. doi: 10.1038/s41564-019-0466-x. Epub 2019 Jun 3.
2019 Sep degradation 7 7 CBM93, GH33, CE3, CE20, GH2, GH20, GH20, CBM32
PUL0116 expression of recombinant proteins, RNA-seq, differential gene expression host glycan Bacteroides thetaiotaomicron <a href=https://pubmed.ncbi.nlm.nih.gov/31160824/>31160824</a>
Complex N-glycan breakdown by gut Bacteroides involves an extensive enzymatic apparatus encoded by multiple co-regulated genetic loci. Nat Microbiol. 2019 Sep;4(9):1571-1581. doi: 10.1038/s41564-019-0466-x. Epub 2019 Jun 3.
2019 Sep degradation 2 1 GH20
PUL0117 expression of recombinant proteins, RNA-seq, differential gene expression, enzyme specificity assay, enzyme activity assay host glycan Bacteroides thetaiotaomicron <a href=https://pubmed.ncbi.nlm.nih.gov/31160824/>31160824</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/23943617/>23943617</a>
Complex N-glycan breakdown by gut Bacteroides involves an extensive enzymatic apparatus encoded by multiple co-regulated genetic loci. Discovery of beta-1,4-D-mannosyl-N-acetyl-D-glucosamine phosphorylase involved in the metabolism of N-glycans. Nat Microbiol. 2019 Sep;4(9):1571-1581. doi: 10.1038/s41564-019-0466-x. Epub 2019 Jun 3. J Biol Chem. 2013 Sep 20;288(38):27366-27374. doi: 10.1074/jbc.M113.469080. Epub 2013 Aug 13.
2019 Sep,2013 Sep 20 degradation 22 7 GH130_2, GH163, GH18, GH20, GH92
PUL0120 expression of recombinant proteins, RNA-seq, differential gene expression host glycan Bacteroides thetaiotaomicron <a href=https://pubmed.ncbi.nlm.nih.gov/31160824/>31160824</a>
Complex N-glycan breakdown by gut Bacteroides involves an extensive enzymatic apparatus encoded by multiple co-regulated genetic loci. Nat Microbiol. 2019 Sep;4(9):1571-1581. doi: 10.1038/s41564-019-0466-x. Epub 2019 Jun 3.
2019 Sep degradation 6 1 GH18
PUL0154 RNA-seq, differential gene expression human milk oligosaccharide Lactobacillus rhamnosus <a href=https://pubmed.ncbi.nlm.nih.gov/30332787/>30332787</a>
Prebiotics for Lactose Intolerance: Variability in Galacto-Oligosaccharide Utilization by Intestinal Lactobacillus rhamnosus. Nutrients. 2018 Oct 16;10(10):1517. doi: 10.3390/nu10101517.
2018 Oct 16 degradation 3 1 GH1
PUL0156 RNA-seq, differential gene expression human milk oligosaccharide Lactobacillus rhamnosus <a href=https://pubmed.ncbi.nlm.nih.gov/30332787/>30332787</a>
Prebiotics for Lactose Intolerance: Variability in Galacto-Oligosaccharide Utilization by Intestinal Lactobacillus rhamnosus. Nutrients. 2018 Oct 16;10(10):1517. doi: 10.3390/nu10101517.
2018 Oct 16 degradation 2 1 GH2
PUL0157 RNA-seq, differential gene expression human milk oligosaccharide Lactobacillus rhamnosus <a href=https://pubmed.ncbi.nlm.nih.gov/30332787/>30332787</a>
Prebiotics for Lactose Intolerance: Variability in Galacto-Oligosaccharide Utilization by Intestinal Lactobacillus rhamnosus. Nutrients. 2018 Oct 16;10(10):1517. doi: 10.3390/nu10101517.
2018 Oct 16 degradation 4 1 GH1
PUL0158 RNA-seq, differential gene expression human milk oligosaccharide Lactobacillus rhamnosus <a href=https://pubmed.ncbi.nlm.nih.gov/30332787/>30332787</a>
Prebiotics for Lactose Intolerance: Variability in Galacto-Oligosaccharide Utilization by Intestinal Lactobacillus rhamnosus. Nutrients. 2018 Oct 16;10(10):1517. doi: 10.3390/nu10101517.
2018 Oct 16 degradation 4 1 GH1
PUL0159 RNA-seq, differential gene expression human milk oligosaccharide Lactobacillus rhamnosus <a href=https://pubmed.ncbi.nlm.nih.gov/30332787/>30332787</a>
Prebiotics for Lactose Intolerance: Variability in Galacto-Oligosaccharide Utilization by Intestinal Lactobacillus rhamnosus. Nutrients. 2018 Oct 16;10(10):1517. doi: 10.3390/nu10101517.
2018 Oct 16 degradation 2 1 GH2
PUL0164 mass spectrometry, sequence homology analysis, differential gene expression beta-mannan Leeuwenhoekiella sp. MAR_2009_132 <a href=https://pubmed.ncbi.nlm.nih.gov/30246424/>30246424</a>
Alpha- and beta-mannan utilization by marine Bacteroidetes. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16.
2018 Nov degradation 19 12 CBM8, CE2, CE20, GH130_1, GH26, GH27, GH3, GH5_2, GH5_7, GH9
PUL0165 mass spectrometry, sequence homology analysis, differential gene expression beta-mannan Salegentibacter sp. Hel_I_6 <a href=https://pubmed.ncbi.nlm.nih.gov/30246424/>30246424</a>
Alpha- and beta-mannan utilization by marine Bacteroidetes. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16.
2018 Nov degradation 16 8 CE20, GH130_1, GH26, GH27, GH30, GH5_2, GH9
PUL0467 microarray, qPCR, expression of recombinant proteins, RNA-seq, differential gene expression host glycan Bacteroides thetaiotaomicron <a href=https://pubmed.ncbi.nlm.nih.gov/18996345/>18996345</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/31160824/>31160824</a>
Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Complex N-glycan breakdown by gut Bacteroides involves an extensive enzymatic apparatus encoded by multiple co-regulated genetic loci. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007. Nat Microbiol. 2019 Sep;4(9):1571-1581. doi: 10.1038/s41564-019-0466-x. Epub 2019 Jun 3.
2008 Nov 13,2019 Sep degradation 14 4 GH18, GH2, GH20, GH29, CBM32
PUL0598 liquid chromatography and mass spectrometry, differential gene expression xylan Clostridium cellulovorans 743B <a href=https://pubmed.ncbi.nlm.nih.gov/26020016/>26020016</a>
Elucidation of the recognition mechanisms for hemicellulose and pectin in Clostridium cellulovorans using intracellular quantitative proteome analysis. AMB Express. 2015 May 23;5:29. doi: 10.1186/s13568-015-0115-6. eCollection 2015.
2015 degradation 4 1 GH95
PUL0599 liquid chromatography and mass spectrometry, differential gene expression xylan Clostridium cellulovorans <a href=https://pubmed.ncbi.nlm.nih.gov/26020016/>26020016</a>
Elucidation of the recognition mechanisms for hemicellulose and pectin in Clostridium cellulovorans using intracellular quantitative proteome analysis. AMB Express. 2015 May 23;5:29. doi: 10.1186/s13568-015-0115-6. eCollection 2015.
2015 degradation 7 1 GH43_11, CBM91
PUL0600 liquid chromatography and mass spectrometry, differential gene expression galactomannan Clostridium cellulovorans <a href=https://pubmed.ncbi.nlm.nih.gov/26020016/>26020016</a>
Elucidation of the recognition mechanisms for hemicellulose and pectin in Clostridium cellulovorans using intracellular quantitative proteome analysis. AMB Express. 2015 May 23;5:29. doi: 10.1186/s13568-015-0115-6. eCollection 2015.
2015 degradation 12 3 GH130_1, GH130_2, GH2
PUL0601 liquid chromatography and mass spectrometry, differential gene expression pectin Clostridium cellulovorans <a href=https://pubmed.ncbi.nlm.nih.gov/26020016/>26020016</a>
Elucidation of the recognition mechanisms for hemicellulose and pectin in Clostridium cellulovorans using intracellular quantitative proteome analysis. AMB Express. 2015 May 23;5:29. doi: 10.1186/s13568-015-0115-6. eCollection 2015.
2015 degradation 15 3 CE4, GH105, GH28
PUL0744 RNA-seq, HPLC, gene mutant, differential gene expression lactose Listeria monocytogenes serotype 4b str. F2365 <a href=https://pubmed.ncbi.nlm.nih.gov/38876592/>38876592</a>
Activation of a silent lactose utilization pathway in an evolved Listeria monocytogenes F2365 outbreak isolate. Food Res Int. 2024 Aug;189:114554. doi: 10.1016/j.foodres.2024.114554. Epub 2024 May 27.
2024 Aug degradation 5 1 GH1
PUL0745 high performance gel permeation chromatography, gas chromatography, RNA-seq, differential gene expression pectic polysaccharide Bacteroides ovatus strain ATCC 8483 <a href=https://pubmed.ncbi.nlm.nih.gov/38890895/>38890895</a>
The Utilization by Bacteroides spp. of a Purified Polysaccharide from Fuzhuan Brick Tea. Foods. 2024 May 26;13(11):1666. doi: 10.3390/foods13111666.
2024 May 26 degradation 12 4 GH146, GH28, GH30_2, PL12
PUL0746 high performance gel permeation chromatography, gas chromatography, RNA-seq, differential gene expression pectic polysaccharide Bacteroides ovatus strain ATCC 8483 <a href=https://pubmed.ncbi.nlm.nih.gov/38890895/>38890895</a>
The Utilization by Bacteroides spp. of a Purified Polysaccharide from Fuzhuan Brick Tea. Foods. 2024 May 26;13(11):1666. doi: 10.3390/foods13111666.
2024 May 26 degradation 7 4 CBM93, GH33, GH2, GH20, GH20, CBM32
PUL0747 high performance gel permeation chromatography, gas chromatography, RNA-seq, differential gene expression pectic polysaccharide Bacteroides ovatus strain ATCC 8483 <a href=https://pubmed.ncbi.nlm.nih.gov/38890895/>38890895</a>
The Utilization by Bacteroides spp. of a Purified Polysaccharide from Fuzhuan Brick Tea. Foods. 2024 May 26;13(11):1666. doi: 10.3390/foods13111666.
2024 May 26 degradation 9 3 GH2, GH20, GH29, CBM32
PUL0771 RNA-seq, differential gene expression, HPAEC-PAD, SDS-PAGE, para-hydroxybenzoic acid (PAHBAH) assay, reducing-sugar assay, Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE), enzyme activity assay fucoidan Rhodopirellula sp. SWK7 <a href=https://pubmed.ncbi.nlm.nih.gov/39738071/>39738071</a>
Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w.
2024 Dec 30 degradation 27 7 GH141, GH168, GH29
PUL0772 RNA-seq, differential gene expression, HPAEC-PAD, SDS-PAGE, para-hydroxybenzoic acid (PAHBAH) assay, reducing-sugar assay, Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE), enzyme activity assay fucoidan Rhodopirellula sp. SWK7 <a href=https://pubmed.ncbi.nlm.nih.gov/39738071/>39738071</a>
Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w.
2024 Dec 30 degradation 9 1 GH29
PUL0773 RNA-seq, differential gene expression, HPAEC-PAD, SDS-PAGE, para-hydroxybenzoic acid (PAHBAH) assay, reducing-sugar assay, Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE), enzyme activity assay fucoidan Rhodopirellula sp. SWK7 <a href=https://pubmed.ncbi.nlm.nih.gov/39738071/>39738071</a>
Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w.
2024 Dec 30 degradation 23 3 GH116, GH29, GH97
PUL0774 RNA-seq, differential gene expression, HPAEC-PAD, SDS-PAGE, para-hydroxybenzoic acid (PAHBAH) assay, reducing-sugar assay, Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE), enzyme activity assay fucoidan Rhodopirellula sp. SWK7 <a href=https://pubmed.ncbi.nlm.nih.gov/39738071/>39738071</a>
Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w.
2024 Dec 30 degradation 23 8 GH117, GH141, GH168, GH29, GH95
PUL0775 RNA-seq, differential gene expression, HPAEC-PAD, SDS-PAGE, para-hydroxybenzoic acid (PAHBAH) assay, reducing-sugar assay, Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE), enzyme activity assay fucoidan Rhodopirellula sp. SWK7 <a href=https://pubmed.ncbi.nlm.nih.gov/39738071/>39738071</a>
Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w.
2024 Dec 30 degradation 14 5 CE14, GH128, GH141, GH29
PUL0776 RNA-seq, differential gene expression, HPAEC-PAD, SDS-PAGE, para-hydroxybenzoic acid (PAHBAH) assay, reducing-sugar assay, Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE), enzyme activity assay fucoidan Rhodopirellula sp. SWK7 <a href=https://pubmed.ncbi.nlm.nih.gov/39738071/>39738071</a>
Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w.
2024 Dec 30 degradation 32 10 CE19, CBM51, CE20, GH115, GH116, GH117, GH117, GH29, GH95
PUL0777 RNA-seq, differential gene expression, HPAEC-PAD, SDS-PAGE, para-hydroxybenzoic acid (PAHBAH) assay, reducing-sugar assay, Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE), enzyme activity assay fucoidan Neorhodopirellula lusitana <a href=https://pubmed.ncbi.nlm.nih.gov/39738071/>39738071</a>
Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w.
2024 Dec 30 degradation 26 3 GH141, GH29
PUL0778 RNA-seq, differential gene expression, HPAEC-PAD, SDS-PAGE, para-hydroxybenzoic acid (PAHBAH) assay, reducing-sugar assay, Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE), enzyme activity assay fucoidan Neorhodopirellula lusitana <a href=https://pubmed.ncbi.nlm.nih.gov/39738071/>39738071</a>
Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w.
2024 Dec 30 degradation 18 4 GH172, GH29, GH3, GH95
PUL0779 RNA-seq, differential gene expression, HPAEC-PAD, SDS-PAGE, para-hydroxybenzoic acid (PAHBAH) assay, reducing-sugar assay, Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE), enzyme activity assay fucoidan Neorhodopirellula lusitana <a href=https://pubmed.ncbi.nlm.nih.gov/39738071/>39738071</a>
Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w.
2024 Dec 30 degradation 19 9 GH107, GH141, GH168, GH29
PUL0780 RNA-seq, differential gene expression, HPAEC-PAD, SDS-PAGE, para-hydroxybenzoic acid (PAHBAH) assay, reducing-sugar assay, Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE), enzyme activity assay fucoidan Neorhodopirellula lusitana <a href=https://pubmed.ncbi.nlm.nih.gov/39738071/>39738071</a>
Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w.
2024 Dec 30 degradation 24 8 CBM32, CE12, CE6, GH141, GH29, GH95
PUL0781 RNA-seq, differential gene expression, HPAEC-PAD, SDS-PAGE, para-hydroxybenzoic acid (PAHBAH) assay, reducing-sugar assay, Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE), enzyme activity assay fucoidan Neorhodopirellula lusitana <a href=https://pubmed.ncbi.nlm.nih.gov/39738071/>39738071</a>
Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w.
2024 Dec 30 degradation 17 4 GH116, GH29, GH95
PUL0782 RNA-seq, differential gene expression, HPAEC-PAD, SDS-PAGE, para-hydroxybenzoic acid (PAHBAH) assay, reducing-sugar assay, Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE), enzyme activity assay fucoidan Neorhodopirellula lusitana <a href=https://pubmed.ncbi.nlm.nih.gov/39738071/>39738071</a>
Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w.
2024 Dec 30 degradation 15 3 GH109, GH117, GH29
PUL0783 RNA-seq, differential gene expression, HPAEC-PAD, SDS-PAGE, para-hydroxybenzoic acid (PAHBAH) assay, reducing-sugar assay, Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE), enzyme activity assay fucoidan Neorhodopirellula lusitana <a href=https://pubmed.ncbi.nlm.nih.gov/39738071/>39738071</a>
Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w.
2024 Dec 30 degradation 20 6 CBM51, GH115, GH172, GH28, GH29, GH95
PUL0784 RNA-seq, differential gene expression, HPAEC-PAD, SDS-PAGE, para-hydroxybenzoic acid (PAHBAH) assay, reducing-sugar assay, Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE), enzyme activity assay fucoidan Neorhodopirellula lusitana <a href=https://pubmed.ncbi.nlm.nih.gov/39738071/>39738071</a>
Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w.
2024 Dec 30 degradation 8 2 GH29, GH95
PUL0785 RNA-seq, differential gene expression, HPAEC-PAD, SDS-PAGE, para-hydroxybenzoic acid (PAHBAH) assay, reducing-sugar assay, Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE), enzyme activity assay fucoidan Neorhodopirellula lusitana <a href=https://pubmed.ncbi.nlm.nih.gov/39738071/>39738071</a>
Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w.
2024 Dec 30 degradation 22 5 CE20, CE20, GH95, CE7, GH117, GH168
PUL0786 RNA-seq, reducing-sugar assay, growth assay, high performance gel permeation chromatography, gas chromatography, RNA-seq, differential gene expression pectic polysaccharide Bacteroides ovatus strain ATCC 8483 <a href=https://pubmed.ncbi.nlm.nih.gov/38890895/>38890895</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/39892338/>39892338</a>
The Utilization by Bacteroides spp. of a Purified Polysaccharide from Fuzhuan Brick Tea. In vitro fermentation of a purified fraction of polysaccharides from the root of Brassica rapa L. by human gut microbiota and its interaction with Bacteroides ovatus. Foods. 2024 May 26;13(11):1666. doi: 10.3390/foods13111666. Food Chem. 2025 May 1;473:143109. doi: 10.1016/j.foodchem.2025.143109. Epub 2025 Jan 27.
2024 May 26,2025 May 1 degradation 26 14 CBM67, GH78, CBM67, GH78, GH33, CE19, GH105, GH130_2, GH140, GH143, GH142, GH163, GH18, GH28, GH43_18, GH92, GH95, PL1_2
PUL0793 enzyme activity assay, quantification of reaction product reducing ends, RNA-seq, differential gene expression, NMR, MALDI-TOF/MS, gas chromatography, mass spectrometry, bicinchoninic acid (BCA) assay, recombinant protein expression arabinan Bacteroides intestinalis DSM 17393 <a href=https://pubmed.ncbi.nlm.nih.gov/39443715/>39443715</a>
In vivo manipulation of human gut Bacteroides fitness by abiotic oligosaccharides. Nat Chem Biol. 2025 Apr;21(4):544-554. doi: 10.1038/s41589-024-01763-6. Epub 2024 Oct 23.
2025 Apr degradation 14 6 CE1, GH127, GH146, GH43_34, CBM32, GH97
PUL0794 enzyme activity assay, quantification of reaction product reducing ends, RNA-seq, differential gene expression, NMR, MALDI-TOF/MS, gas chromatography, mass spectrometry, bicinchoninic acid (BCA) assay, recombinant protein expression arabinan Bacteroides intestinalis DSM 17393 <a href=https://pubmed.ncbi.nlm.nih.gov/39443715/>39443715</a>
In vivo manipulation of human gut Bacteroides fitness by abiotic oligosaccharides. Nat Chem Biol. 2025 Apr;21(4):544-554. doi: 10.1038/s41589-024-01763-6. Epub 2024 Oct 23.
2025 Apr degradation 23 8 GH146, GH28, GH43_29, GH43_4, GH51_1, GH51_2, GH97