dbCAN-PUL

Browse dbCAN-PUL Entries

PULID Characterization Method(s) Substrate Organism Publication Publish Date Type Num Genes Num CAZymes CazyFamily
PUL0051 sequence homology analysis, NMR, size-exclusion chromatography (SEC), clone and expression, recombinant protein expression, enzyme kinetic analysis alginate Cellulophaga lytica 29795267
Ancient acquisition of "alginate utilization loci" by human gut microbiota. Sci Rep. 2018 May 23;8(1):8075. doi: 10.1038/s41598-018-26104-1.
2018 May 23 degradation 6 2 PL17_2, PL17, PL6, PL6_1
PUL0052 sequence homology analysis, NMR, size-exclusion chromatography (SEC), clone and expression, recombinant protein expression, enzyme kinetic analysis alginate Maricaulis maris 29795267
Ancient acquisition of "alginate utilization loci" by human gut microbiota. Sci Rep. 2018 May 23;8(1):8075. doi: 10.1038/s41598-018-26104-1.
2018 May 23 degradation 10 2 PL17_2, PL17, PL6, PL6_1
PUL0053 sequence homology analysis, NMR, size-exclusion chromatography (SEC), clone and expression, recombinant protein expression, enzyme kinetic analysis alginate Stenotrophomonas maltophilia 29795267
Ancient acquisition of "alginate utilization loci" by human gut microbiota. Sci Rep. 2018 May 23;8(1):8075. doi: 10.1038/s41598-018-26104-1.
2018 May 23 degradation 8 2 PL17_2, PL17, PL6
PUL0054 sequence homology analysis, NMR, size-exclusion chromatography (SEC), clone and expression, recombinant protein expression, enzyme kinetic analysis alginate Alteromonas macleodii 29795267
Ancient acquisition of "alginate utilization loci" by human gut microbiota. Sci Rep. 2018 May 23;8(1):8075. doi: 10.1038/s41598-018-26104-1.
2018 May 23 degradation 9 2 PL17_2, PL17, PL6, PL6_1
PUL0055 sequence homology analysis, NMR, size-exclusion chromatography (SEC), clone and expression, recombinant protein expression, enzyme kinetic analysis alginate Bacteroides sp. 1_1_30 29795267
Ancient acquisition of "alginate utilization loci" by human gut microbiota. Sci Rep. 2018 May 23;8(1):8075. doi: 10.1038/s41598-018-26104-1.
2018 May 23 degradation 7 2 PL17_2, PL17, PL6, PL6_1
PUL0056 sequence homology analysis, NMR, size-exclusion chromatography (SEC), clone and expression, recombinant protein expression, enzyme kinetic analysis alginate Bacteroides eggerthii 29795267
Ancient acquisition of "alginate utilization loci" by human gut microbiota. Sci Rep. 2018 May 23;8(1):8075. doi: 10.1038/s41598-018-26104-1.
2018 May 23 degradation 5 3 CE20, PL17_2, PL17, PL6, PL6_1
PUL0151 sequence homology analysis, Northern Blot, RT-qPCR, electrophoretic mobility shift assay, clone and expression, gene deletion mutant and growth assay alginate Zobellia galactanivorans 30524390
Evolutionary Evidence of Algal Polysaccharide Degradation Acquisition by Pseudoalteromonas carrageenovora 9(T) to Adapt to Macroalgal Niches. Regulation of alginate catabolism involves a GntR family repressor in the marine flavobacterium Zobellia galactanivorans DsijT. Front Microbiol. 2018 Nov 22;9:2740. doi: 10.3389/fmicb.2018.02740. eCollection 2018. Nucleic Acids Res. 2020 Aug 20;48(14):7786-7800. doi: 10.1093/nar/gkaa533.
2018,2020 Aug 20 degradation 12 2 PL17_2, PL17, PL7
PUL0208 growth assay, clone and expression, enzyme activity assay chitin Pseudoalteromonas luteoviolacea 31213521
Marine Chitinolytic Pseudoalteromonas Represents an Untapped Reservoir of Bioactive Potential. Multiple genes involved in chitin degradation from the marine bacterium Pseudoalteromonas sp. strain S91. mSystems. 2019 Jun 18;4(4):e00060-19. doi: 10.1128/mSystems.00060-19. Microbiology (Reading). 1999 Apr;145 ( Pt 4):925-934. doi: 10.1099/13500872-145-4-925.
2019 Jun 18,1999 Apr degradation 3 3 AA10, CBM5, CBM5, GH18, GH18, CBM5, CBM5
PUL0313 microarray, Northern Blot, RT-qPCR, electrophoretic mobility shift assay, clone and expression, gene deletion mutant and growth assay alginate Zobellia galactanivorans 28983288
Gene Expression Analysis of Zobellia galactanivorans during the Degradation of Algal Polysaccharides Reveals both Substrate-Specific and Shared Transcriptome-Wide Responses. Regulation of alginate catabolism involves a GntR family repressor in the marine flavobacterium Zobellia galactanivorans DsijT. Front Microbiol. 2017 Sep 21;8:1808. doi: 10.3389/fmicb.2017.01808. eCollection 2017. Nucleic Acids Res. 2020 Aug 20;48(14):7786-7800. doi: 10.1093/nar/gkaa533.
2017,2020 Aug 20 degradation 3 3 PL6, PL6_1, PL7_5
PUL0380 microarray, qPCR, microarray, enzyme activity assay, strcutural analysis, clone and expression mucin Bacteroides thetaiotaomicron 18996345
Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Functional genomic and metabolic studies of the adaptations of a prominent adult human gut symbiont, Bacteroides thetaiotaomicron, to the suckling period. Structural basis of mammalian high-mannose N-glycan processing by human gut Bacteroides. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007. J Biol Chem. 2006 Nov 24;281(47):36269-79. doi: 10.1074/jbc.M606509200. Epub 2006 Sep 12. Nat Commun. 2020 Feb 14;11(1):899. doi: 10.1038/s41467-020-14754-7.
2008 Nov 13,2006 Nov 24,2020 Feb 14 degradation 12 4 GH18, GH92
PUL0412 clone and expression, enzyme activity assay pectin Ralstonia solanacearum 12795379
Characterization of a Ralstonia solanacearum operon required for polygalacturonate degradation and uptake of galacturonic acid. Mol Plant Microbe Interact. 2003 Jun;16(6):536-44. doi: 10.1094/MPMI.2003.16.6.536.
2003 Jun degradation 2 1 GH28
PUL0423 clone and expression, enzyme activity assay cellobiose Thermotoga neapolitana 10960102
Cloning and characterization of the glucooligosaccharide catabolic pathway beta-glucan glucohydrolase and cellobiose phosphorylase in the marine hyperthermophile Thermotoga neapolitana. J Bacteriol. 2000 Sep;182(18):5172-9. doi: 10.1128/JB.182.18.5172-5179.2000.
2000 Sep degradation 3 2 GH1, GH94
PUL0455 clone and expression, genes induced in presence of substrate, enzyme activity assay starch Bifidobacterium animalis 12513973
Induction of sucrose utilization genes from Bifidobacterium lactis by sucrose and raffinose. Appl Environ Microbiol. 2003 Jan;69(1):24-32. doi: 10.1128/AEM.69.1.24-32.2003.
2003 Jan degradation 3 1 GH13_18
PUL0466 clone and expression, enzyme activity assay, Northern Blot arabinan Bacillus subtilis 14973026
Transcriptional regulation of genes encoding arabinan-degrading enzymes in Bacillus subtilis. J Bacteriol. 2004 Mar;186(5):1287-96. doi: 10.1128/JB.186.5.1287-1296.2004.
2004 Mar degradation 9 1 GH51_1
PUL0475 clone and expression, gene deletion mutant and growth assay beta-glucan Streptomyces reticuli 10347054
Characterization of the binding protein-dependent cellobiose and cellotriose transport system of the cellulose degrader Streptomyces reticuli. Appl Environ Microbiol. 1999 Jun;65(6):2636-43. doi: 10.1128/AEM.65.6.2636-2643.1999.
1999 Jun degradation 7 2 CBM2, GH18, GH1
PUL0497 clone and expression, enzyme activity assay chitin Pseudoalteromonas piscicida 11772635
Identification and characterization of the gene cluster involved in chitin degradation in a marine bacterium, Alteromonas sp. strain O-7. Appl Environ Microbiol. 2002 Jan;68(1):263-70. doi: 10.1128/AEM.68.1.263-270.2002.
2002 Jan degradation 3 3 AA10, CBM5, GH18, GH18, CBM5
PUL0508 clone and expression, enzyme activity assay xylan Streptomyces thermoviolaceus 14761997
Molecular characterization of a high-affinity xylobiose transporter of Streptomyces thermoviolaceus OPC-520 and its transcriptional regulation. J Bacteriol. 2004 Feb;186(4):1029-37. doi: 10.1128/JB.186.4.1029-1037.2004.
2004 Feb degradation 5 1 GH3
PUL0520 clone and expression, enzyme activity assay xylan Klebsiella oxytoca 14532050
Cloning, characterization, and functional expression of the Klebsiella oxytoca xylodextrin utilization operon (xynTB) in Escherichia coli. Appl Environ Microbiol. 2003 Oct;69(10):5957-67. doi: 10.1128/AEM.69.10.5957-5967.2003.
2003 Oct degradation 2 1 GH43_11, CBM91
PUL0531 clone and expression, enzyme activity assay chitin Serratia marcescens 12618440
Uptake of N,N'-diacetylchitobiose [(GlcNAc)2] via the phosphotransferase system is essential for chitinase production by Serratia marcescens 2170. J Bacteriol. 2003 Mar;185(6):1776-82. doi: 10.1128/JB.185.6.1776-1782.2003.
2003 Mar degradation 5 1 GH1
PUL0561 clone and expression, enzyme activity assay alpha-galactan Lactobacillus plantarum 12406739
Characterization of the melA locus for alpha-galactosidase in Lactobacillus plantarum. Appl Environ Microbiol. 2002 Nov;68(11):5464-71. doi: 10.1128/AEM.68.11.5464-5471.2002.
2002 Nov degradation 5 2 GH2, GH36
PUL0568 clone and expression, enzyme activity assay, Northern Blot starch Clostridium beijerinckii 10411273
The genes controlling sucrose utilization in Clostridium beijerinckii NCIMB 8052 constitute an operon. Microbiology (Reading). 1999 Jun;145 ( Pt 6):1461-1472. doi: 10.1099/13500872-145-6-1461.
1999 Jun degradation 4 1 GH32
PUL0569 clone and expression, enzyme activity assay, Northern Blot fructan Bacillus subtilis 11739774
yveB, Encoding endolevanase LevB, is part of the sacB-yveB-yveA levansucrase tricistronic operon in Bacillus subtilis. Microbiology (Reading). 2001 Dec;147(Pt 12):3413-9. doi: 10.1099/00221287-147-12-3413.
2001 Dec degradation 4 2 GH32, GH68_1
PUL0570 clone and expression, enzyme activity assay cellobiose Corynebacterium glutamicum 12777497
A single V317A or V317M substitution in Enzyme II of a newly identified beta-glucoside phosphotransferase and utilization system of Corynebacterium glutamicum R extends its specificity towards cellobiose. Microbiology (Reading). 2003 Jun;149(Pt 6):1569-1580. doi: 10.1099/mic.0.26053-0.
2003 Jun degradation 3 1 GH1
PUL0606 enzyme activity assay, clone and expression beta-galactooligosaccharide Bifidobacterium breve UCC2003 32385941
Biochemical analysis of cross-feeding behaviour between two common gut commensals when cultivated on plant-derived arabinogalactan. Microb Biotechnol. 2020 Nov;13(6):1733-1747. doi: 10.1111/1751-7915.13577. Epub 2020 May 9.
2020 Nov degradation 3 1 GH2
PUL0607 enzyme activity assay, clone and expression, liquid chromatography and mass spectrometry agarose Wenyingzhuangia fucanilytica strain CZ1127 32520542
Characterization of a Novel Porphyranase Accommodating Methyl-galactoses at Its Subsites. J Agric Food Chem. 2020 Jul 1;68(26):7032-7039. doi: 10.1021/acs.jafc.0c02404. Epub 2020 Jun 22.
2020 Jul 1 degradation 22 8 GH105, GH154, GH117, GH141, GH16_11, GH16_14, GH2, GH29
PUL0608 enzyme activity assay, clone and expression, liquid chromatography and mass spectrometry, thin-layer chromatography, MALDI-TOF/MS human milk oligosaccharide Roseburia hominis DSM 16839 32620774
Butyrate producing colonic Clostridiales metabolise human milk oligosaccharides and cross feed on mucin via conserved pathways. Nat Commun. 2020 Jul 3;11(1):3285. doi: 10.1038/s41467-020-17075-x.
2020 Jul 3 degradation 9 2 GH112, GH136
PUL0609 enzyme activity assay, clone and expression, liquid chromatography and mass spectrometry, thin-layer chromatography, MALDI-TOF/MS human milk oligosaccharide Roseburia inulinivorans DSM 16841 32620774
Butyrate producing colonic Clostridiales metabolise human milk oligosaccharides and cross feed on mucin via conserved pathways. Nat Commun. 2020 Jul 3;11(1):3285. doi: 10.1038/s41467-020-17075-x.
2020 Jul 3 degradation 11 4 GH112, GH136, CBM32, GH95
PUL0662 thin-layer chromatography, liquid chromatography and mass spectrometry, qPCR, clone and expression beta-mannan Phocaeicola dorei DSM 17855 34339781
BdPUL12 depolymerizes beta-mannan-like glycans into mannooligosaccharides and mannose, which serve as carbon sources for Bacteroides dorei and gut probiotics. Int J Biol Macromol. 2021 Sep 30;187:664-674. doi: 10.1016/j.ijbiomac.2021.07.172. Epub 2021 Jul 31.
2021 Sep 30 degradation 8 4 CE7, GH130_1, GH26, GH5_7
PUL0663 thin-layer chromatography, clone and expression, recombinant protein expression arabinogalactan Bacteroides plebeius DSM17135 34340552
Sulfation of Arabinogalactan Proteins Confers Privileged Nutrient Status to Bacteroides plebeius. mBio. 2021 Aug 31;12(4):e0136821. doi: 10.1128/mBio.01368-21. Epub 2021 Aug 3.
2021 Aug 31 degradation 7 4 GH154, GH43_17, GH43_24, PL42
PUL0664 thin-layer chromatography, clone and expression, recombinant protein expression arabinogalactan Bacteroides plebeius DSM17135 34340552
Sulfation of Arabinogalactan Proteins Confers Privileged Nutrient Status to Bacteroides plebeius. mBio. 2021 Aug 31;12(4):e0136821. doi: 10.1128/mBio.01368-21. Epub 2021 Aug 3.
2021 Aug 31 degradation 16 8 GH2, GH27, GH36, GH43, GH49
PUL0680 gene deletion mutant and growth assay, clone and expression, qRT-PCR, high-performance anion-exchange chromatography, crystallization, recombinant protein expression xyloglucan Bacteroides uniformis ATCC 8492 34995484
Polysaccharide utilization loci in Bacteroides determine population fitness and community-level interactions. Cell Surface Xyloglucan Recognition and Hydrolysis by the Human Gut Commensal Bacteroides uniformis. Cell Host Microbe. 2022 Feb 9;30(2):200-215.e12. doi: 10.1016/j.chom.2021.12.006. Epub 2022 Jan 6. Appl Environ Microbiol. 2022 Jan 11;88(1):e0156621. doi: 10.1128/AEM.01566-21. Epub 2021 Nov 3.
2022 Feb 9,2022 Jan 11 degradation 15 10 CE20, GH43_16, CBM6, GH2, GH29, GH2, GH2, GH31_4, GH42, GH43_33, GH5_4, GH97
PUL0688 clone and expression, crystallization, recombinant protein expression, thin-layer chromatography galactooligosaccharide Bacteroides thetaiotaomicron VPI-5482 34149636
Analysis of Two SusE-Like Enzymes From Bacteroides thetaiotaomicron Reveals a Potential Degradative Capacity for This Protein Family. Front Microbiol. 2021 Jun 4;12:645765. doi: 10.3389/fmicb.2021.645765. eCollection 2021.
2021 degradation 5 1 GH95
PUL0689 clone and expression, crystallization, recombinant protein expression, thin-layer chromatography galactooligosaccharide Bacteroides thetaiotaomicron VPI-5482 34149636
Analysis of Two SusE-Like Enzymes From Bacteroides thetaiotaomicron Reveals a Potential Degradative Capacity for This Protein Family. Dynamic genetic adaptation of Bacteroides thetaiotaomicron during murine gut colonization. Front Microbiol. 2021 Jun 4;12:645765. doi: 10.3389/fmicb.2021.645765. eCollection 2021. Cell Rep. 2023 Aug 29;42(8):113009. doi: 10.1016/j.celrep.2023.113009. Epub 2023 Aug 21.
2021,2023 Aug 29 degradation 10 4 GH2, CBM32, GH3, GH36, GH43_10, CBM91
PUL0698 clone and expression, high-performance anion-exchange chromatography, crystallization beta-mannan Muricauda sp. MAR_2010_75 36411326
Marine bacteroidetes use a conserved enzymatic cascade to digest diatom beta-mannan. ISME J. 2023 Feb;17(2):276-285. doi: 10.1038/s41396-022-01342-4. Epub 2022 Nov 21.
2023 Feb degradation 22 8 CE2, GH130_1, GH26, GH27, GH5_26, GH9
PUL0704 fluorophore-assisted carbohydrate electrophoresis (FACE), dinitrosalicylic acid-assay (DNS-assay), HPLC, clone and expression xylan Flavimarina sp. Hel_I_48 37121608
Marine Bacteroidetes enzymatically digest xylans from terrestrial plants. Environ Microbiol. 2023 Sep;25(9):1713-1727. doi: 10.1111/1462-2920.16390. Epub 2023 Apr 30.
2023 Sep degradation 18 7 CE15, CBM9, CE20, CE20, GH10, GH115, GH115, GH43_1, GH67
PUL0705 fluorophore-assisted carbohydrate electrophoresis (FACE), dinitrosalicylic acid-assay (DNS-assay), HPLC, clone and expression xylan Flavimarina sp. Hel_I_48 37121608
Marine Bacteroidetes enzymatically digest xylans from terrestrial plants. Environ Microbiol. 2023 Sep;25(9):1713-1727. doi: 10.1111/1462-2920.16390. Epub 2023 Apr 30.
2023 Sep degradation 14 8 CE6, CE1, CE1, GH10, GH43_10, CBM91, GH43_12, CBM91, GH8, GH95, GH97
PUL0714 clone and expression, crystallization, isothermal titration calorimetry (ITC), thin-layer chromatography, Western Blot, gene mutant starch Bacteroides ovatus strain ATCC 8483  37500984
BoGH13A(Sus) from Bacteroides ovatus represents a novel alpha-amylase used for Bacteroides starch breakdown in the human gut. Cell Mol Life Sci. 2023 Jul 28;80(8):232. doi: 10.1007/s00018-023-04812-w.
2023 Jul 28 degradation 8 3 GH13_10, GH13_46, GH97
PUL0715 clone and expression, enzyme activity assay, ultra-performance liquid chromatography–mass spectrometry(UPLC-MS) alginate Wenyingzhuangia fucanilytica CZ1127 37540808
A repertoire of alginate lyases in the alginate polysaccharide utilization loci of marine bacterium Wenyingzhuangia fucanilytica: biochemical properties and action pattern. J Sci Food Agric. 2024 Jan 15;104(1):134-140. doi: 10.1002/jsfa.12898. Epub 2023 Aug 23.
2024 Jan 15 degradation 12 4 PL17_2, PL17, PL6, PL6_1, PL7
PUL0725 NMR, clone and expression, enzyme kinetic analysis alpha-glucan Marinovum sp. 38180643
A Broad-Spectrum alpha-Glucosidase of Glycoside Hydrolase Family 13 from Marinovum sp., a Member of the Roseobacter Clade. Appl Biochem Biotechnol. 2024 Sep;196(9):6059-6071. doi: 10.1007/s12010-023-04820-3. Epub 2024 Jan 5.
2024 Sep degradation 9 1 GH13_23
PUL0726 reducing-sugar assay, NMR, clone and expression, liquid chromatography and mass spectrometry, mass spectrometry sulfosugar Agrobacterium tumefaciens str. C58 35074914
Oxidative desulfurization pathway for complete catabolism of sulfoquinovose by bacteria. Proc Natl Acad Sci U S A. 2022 Jan 25;119(4):e2116022119. doi: 10.1073/pnas.2116022119.
2022 Jan 25 degradation 9 1 GH31_13
PUL0728 HPAEC-PAD, SDS-PAGE, clone and expression human milk oligosaccharide Akkermansia muciniphila CSUN-19 38299857
Mechanism of 2'-fucosyllactose degradation by human-associated Akkermansia. J Bacteriol. 2024 Feb 22;206(2):e0033423. doi: 10.1128/jb.00334-23. Epub 2024 Feb 1.
2024 Feb 22 degradation 18 5 CBM50, GH27, GH29, GH2, CBM32, GH33
PUL0730 enzyme activity assay, recombinant protein expression, NMR, HPLC, clone and expression carrageenan Cellulophaga algicola DSM 14237 38442258
Biocatalytic Conversion of Carrageenans for the Production of 3,6-Anhydro-D-galactose. J Agric Food Chem. 2024 Mar 20;72(11):5816-5827. doi: 10.1021/acs.jafc.3c08613. Epub 2024 Mar 5.
2024 Mar 20 degradation 17 3 GH127, GH16_13
PUL0731 enzyme activity assay, recombinant protein expression, NMR, HPLC, clone and expression carrageenan Saccharicrinis fermentans DSM 9555 38442258
Biocatalytic Conversion of Carrageenans for the Production of 3,6-Anhydro-D-galactose. J Agric Food Chem. 2024 Mar 20;72(11):5816-5827. doi: 10.1021/acs.jafc.3c08613. Epub 2024 Mar 5.
2024 Mar 20 degradation 20 5 GH110, GH127, GH167, GH2
PUL0732 enzyme activity assay, recombinant protein expression, NMR, HPLC, clone and expression carrageenan Cellulophaga baltica 18 38442258
Biocatalytic Conversion of Carrageenans for the Production of 3,6-Anhydro-D-galactose. J Agric Food Chem. 2024 Mar 20;72(11):5816-5827. doi: 10.1021/acs.jafc.3c08613. Epub 2024 Mar 5.
2024 Mar 20 degradation 19 3 GH127, GH16_13
PUL0733 enzyme activity assay, recombinant protein expression, NMR, HPLC, clone and expression carrageenan Echinicola pacifica DSM 19836 38442258
Biocatalytic Conversion of Carrageenans for the Production of 3,6-Anhydro-D-galactose. J Agric Food Chem. 2024 Mar 20;72(11):5816-5827. doi: 10.1021/acs.jafc.3c08613. Epub 2024 Mar 5.
2024 Mar 20 degradation 16 2 GH127, GH129
PUL0734 enzyme activity assay, recombinant protein expression, NMR, HPLC, clone and expression carrageenan Cellulophaga lytica DSM 7489 38442258
Biocatalytic Conversion of Carrageenans for the Production of 3,6-Anhydro-D-galactose. J Agric Food Chem. 2024 Mar 20;72(11):5816-5827. doi: 10.1021/acs.jafc.3c08613. Epub 2024 Mar 5.
2024 Mar 20 degradation 20 3 GH127, GH129, GH2
PUL0735 enzyme activity assay, clone and expression, reducing-sugar assay, thin-layer chromatography, SDS-PAGE arabinan Bifidobacterium longum subsp. suis DSM 20211 38542148
Functional Characterization of Endo- and Exo-Hydrolase Genes in Arabinan Degradation Gene Cluster of Bifidobacterium longum subsp. suis. Int J Mol Sci. 2024 Mar 9;25(6):3175. doi: 10.3390/ijms25063175.
2024 Mar 9 degradation 17 7 GH127, GH27, GH43_26, GH43_27, GH43_4, GH51_2