Browse dbCAN-PUL Entries
| PULID |
Characterization Method(s) |
Substrate |
Organism |
Publication |
Publish Date |
Type |
Num Genes |
Num CAZymes |
CazyFamily |
| PUL0720 |
HPAEC-PAD,
enzyme kinetic analysis,
thin-layer chromatography,
recombinant protein expression,
gene mutant
|
arabinan
|
Bifidobacterium longum JCM 1217 |
<a href=https://pubmed.ncbi.nlm.nih.gov/24385433/>24385433</a>
Characterization of a novel beta-L-arabinofuranosidase in Bifidobacterium longum: functional elucidation of a DUF1680 protein family member. J Biol Chem. 2014 Feb 21;289(8):5240-9. doi: 10.1074/jbc.M113.528711. Epub 2014 Jan 2. |
2014 Feb 21 |
degradation |
7 |
3 |
GH121,
GH127,
GH43_29
|
| PUL0728 |
HPAEC-PAD,
SDS-PAGE,
clone and expression
|
human milk oligosaccharide
|
Akkermansia muciniphila CSUN-19 |
<a href=https://pubmed.ncbi.nlm.nih.gov/38299857/>38299857</a>
Mechanism of 2'-fucosyllactose degradation by human-associated Akkermansia. J Bacteriol. 2024 Feb 22;206(2):e0033423. doi: 10.1128/jb.00334-23. Epub 2024 Feb 1. |
2024 Feb 22 |
degradation |
18 |
5 |
CBM50,
GH27,
GH29,
GH2,
CBM32,
GH33
|
| PUL0743 |
gene mutant,
SDS-PAGE,
Western Blot,
recombinant protein expression,
thermal shift assay (TSA),
isothermal titration calorimetry (ITC),
HPAEC-PAD,
RT-qPCR,
fluorescence measurements
|
xylan
|
Ruminiclostridium cellulolyticum H10 |
<a href=https://pubmed.ncbi.nlm.nih.gov/36403068/>36403068</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/38789996/>38789996</a>
Selfish uptake versus extracellular arabinoxylan degradation in the primary degrader Ruminiclostridium cellulolyticum, a new string to its bow. Intracellular removal of acetyl, feruloyl and p-coumaroyl decorations on arabinoxylo-oligosaccharides imported from lignocellulosic biomass degradation by Ruminiclostridium cellulolyticum. Biotechnol Biofuels Bioprod. 2022 Nov 19;15(1):127. doi: 10.1186/s13068-022-02225-8. Microb Cell Fact. 2024 May 24;23(1):151. doi: 10.1186/s12934-024-02423-z. |
2022 Nov 19,2024 May 24 |
degradation |
13 |
6 |
CE1,
CE20,
CE20,
GH39,
GH43_10,
CBM91,
GH51_1,
GH8
|
| PUL0748 |
Reducing End Assay,
HPAEC-PAD
|
glycogen
|
Pontibacter sp. SGAir0037 |
<a href=https://pubmed.ncbi.nlm.nih.gov/38930854/>38930854</a>
Characterization of Two Glycoside Hydrolases of Family GH13 and GH57, Present in a Polysaccharide Utilization Locus (PUL) of Pontibacter sp. SGAir0037. Molecules. 2024 Jun 12;29(12):2788. doi: 10.3390/molecules29122788. |
2024 Jun 12 |
degradation |
14 |
10 |
CBM48,
GH13_10,
CBM48,
GH13_9,
GH13,
GH13_16,
GH13_26,
GH13_3,
GH57,
GH77,
GH97
|
| PUL0750 |
RNA-seq,
BCA assay,
pNP glycoside assay,
HPAEC-PAD,
qRT-PCR,
gas chromatography,
mass spectrometry,
thin-layer chromatography
|
beta-glucan
|
Segatella copri DSM 18205 |
<a href=https://pubmed.ncbi.nlm.nih.gov/39122003/>39122003</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/39636128/>39636128</a>
The molecular basis of cereal mixed-linkage beta-glucan utilization by the human gut bacterium Segatella copri. Transcriptional delineation of polysaccharide utilization loci in the human gut commensal Segatella copri DSM18205 and co-culture with exemplar Bacteroides species on dietary plant glycans. J Biol Chem. 2024 Sep;300(9):107625. doi: 10.1016/j.jbc.2024.107625. Epub 2024 Aug 8. Appl Environ Microbiol. 2025 Jan 31;91(1):e0175924. doi: 10.1128/aem.01759-24. Epub 2024 Dec 5. |
2024 Sep,2025 Jan 31 |
degradation |
11 |
3 |
GH3,
GH5_4,
GH94
|
| PUL0752 |
thin-layer chromatography,
HPAEC-PAD,
recombinant protein expression,
in vitro assimilation
|
fructo-disaccharide
|
Blautia parvula NBRC 113351 |
<a href=https://pubmed.ncbi.nlm.nih.gov/39500763/>39500763</a>
Degradation mechanism of difructose dianhydride III in Blautia species. Appl Microbiol Biotechnol. 2024 Nov 5;108(1):502. doi: 10.1007/s00253-024-13346-5. |
2024 Nov 5 |
degradation |
10 |
3 |
GH32,
GH39,
GH91
|
| PUL0771 |
RNA-seq,
differential gene expression,
HPAEC-PAD,
SDS-PAGE,
para-hydroxybenzoic acid (PAHBAH) assay,
reducing-sugar assay,
Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE),
enzyme activity assay
|
fucoidan
|
Rhodopirellula sp. SWK7 |
<a href=https://pubmed.ncbi.nlm.nih.gov/39738071/>39738071</a>
Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w. |
2024 Dec 30 |
degradation |
27 |
7 |
GH141,
GH168,
GH29
|
| PUL0772 |
RNA-seq,
differential gene expression,
HPAEC-PAD,
SDS-PAGE,
para-hydroxybenzoic acid (PAHBAH) assay,
reducing-sugar assay,
Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE),
enzyme activity assay
|
fucoidan
|
Rhodopirellula sp. SWK7 |
<a href=https://pubmed.ncbi.nlm.nih.gov/39738071/>39738071</a>
Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w. |
2024 Dec 30 |
degradation |
9 |
1 |
GH29
|
| PUL0773 |
RNA-seq,
differential gene expression,
HPAEC-PAD,
SDS-PAGE,
para-hydroxybenzoic acid (PAHBAH) assay,
reducing-sugar assay,
Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE),
enzyme activity assay
|
fucoidan
|
Rhodopirellula sp. SWK7 |
<a href=https://pubmed.ncbi.nlm.nih.gov/39738071/>39738071</a>
Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w. |
2024 Dec 30 |
degradation |
23 |
3 |
GH116,
GH29,
GH97
|
| PUL0774 |
RNA-seq,
differential gene expression,
HPAEC-PAD,
SDS-PAGE,
para-hydroxybenzoic acid (PAHBAH) assay,
reducing-sugar assay,
Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE),
enzyme activity assay
|
fucoidan
|
Rhodopirellula sp. SWK7 |
<a href=https://pubmed.ncbi.nlm.nih.gov/39738071/>39738071</a>
Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w. |
2024 Dec 30 |
degradation |
23 |
8 |
GH117,
GH141,
GH168,
GH29,
GH95
|
| PUL0775 |
RNA-seq,
differential gene expression,
HPAEC-PAD,
SDS-PAGE,
para-hydroxybenzoic acid (PAHBAH) assay,
reducing-sugar assay,
Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE),
enzyme activity assay
|
fucoidan
|
Rhodopirellula sp. SWK7 |
<a href=https://pubmed.ncbi.nlm.nih.gov/39738071/>39738071</a>
Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w. |
2024 Dec 30 |
degradation |
14 |
5 |
CE14,
GH128,
GH141,
GH29
|
| PUL0776 |
RNA-seq,
differential gene expression,
HPAEC-PAD,
SDS-PAGE,
para-hydroxybenzoic acid (PAHBAH) assay,
reducing-sugar assay,
Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE),
enzyme activity assay
|
fucoidan
|
Rhodopirellula sp. SWK7 |
<a href=https://pubmed.ncbi.nlm.nih.gov/39738071/>39738071</a>
Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w. |
2024 Dec 30 |
degradation |
32 |
10 |
CE19,
CBM51,
CE20,
GH115,
GH116,
GH117,
GH117,
GH29,
GH95
|
| PUL0777 |
RNA-seq,
differential gene expression,
HPAEC-PAD,
SDS-PAGE,
para-hydroxybenzoic acid (PAHBAH) assay,
reducing-sugar assay,
Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE),
enzyme activity assay
|
fucoidan
|
Neorhodopirellula lusitana |
<a href=https://pubmed.ncbi.nlm.nih.gov/39738071/>39738071</a>
Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w. |
2024 Dec 30 |
degradation |
26 |
3 |
GH141,
GH29
|
| PUL0778 |
RNA-seq,
differential gene expression,
HPAEC-PAD,
SDS-PAGE,
para-hydroxybenzoic acid (PAHBAH) assay,
reducing-sugar assay,
Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE),
enzyme activity assay
|
fucoidan
|
Neorhodopirellula lusitana |
<a href=https://pubmed.ncbi.nlm.nih.gov/39738071/>39738071</a>
Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w. |
2024 Dec 30 |
degradation |
18 |
4 |
GH172,
GH29,
GH3,
GH95
|
| PUL0779 |
RNA-seq,
differential gene expression,
HPAEC-PAD,
SDS-PAGE,
para-hydroxybenzoic acid (PAHBAH) assay,
reducing-sugar assay,
Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE),
enzyme activity assay
|
fucoidan
|
Neorhodopirellula lusitana |
<a href=https://pubmed.ncbi.nlm.nih.gov/39738071/>39738071</a>
Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w. |
2024 Dec 30 |
degradation |
19 |
9 |
GH107,
GH141,
GH168,
GH29
|
| PUL0780 |
RNA-seq,
differential gene expression,
HPAEC-PAD,
SDS-PAGE,
para-hydroxybenzoic acid (PAHBAH) assay,
reducing-sugar assay,
Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE),
enzyme activity assay
|
fucoidan
|
Neorhodopirellula lusitana |
<a href=https://pubmed.ncbi.nlm.nih.gov/39738071/>39738071</a>
Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w. |
2024 Dec 30 |
degradation |
24 |
8 |
CBM32,
CE12,
CE6,
GH141,
GH29,
GH95
|
| PUL0781 |
RNA-seq,
differential gene expression,
HPAEC-PAD,
SDS-PAGE,
para-hydroxybenzoic acid (PAHBAH) assay,
reducing-sugar assay,
Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE),
enzyme activity assay
|
fucoidan
|
Neorhodopirellula lusitana |
<a href=https://pubmed.ncbi.nlm.nih.gov/39738071/>39738071</a>
Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w. |
2024 Dec 30 |
degradation |
17 |
4 |
GH116,
GH29,
GH95
|
| PUL0782 |
RNA-seq,
differential gene expression,
HPAEC-PAD,
SDS-PAGE,
para-hydroxybenzoic acid (PAHBAH) assay,
reducing-sugar assay,
Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE),
enzyme activity assay
|
fucoidan
|
Neorhodopirellula lusitana |
<a href=https://pubmed.ncbi.nlm.nih.gov/39738071/>39738071</a>
Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w. |
2024 Dec 30 |
degradation |
15 |
3 |
GH109,
GH117,
GH29
|
| PUL0783 |
RNA-seq,
differential gene expression,
HPAEC-PAD,
SDS-PAGE,
para-hydroxybenzoic acid (PAHBAH) assay,
reducing-sugar assay,
Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE),
enzyme activity assay
|
fucoidan
|
Neorhodopirellula lusitana |
<a href=https://pubmed.ncbi.nlm.nih.gov/39738071/>39738071</a>
Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w. |
2024 Dec 30 |
degradation |
20 |
6 |
CBM51,
GH115,
GH172,
GH28,
GH29,
GH95
|
| PUL0784 |
RNA-seq,
differential gene expression,
HPAEC-PAD,
SDS-PAGE,
para-hydroxybenzoic acid (PAHBAH) assay,
reducing-sugar assay,
Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE),
enzyme activity assay
|
fucoidan
|
Neorhodopirellula lusitana |
<a href=https://pubmed.ncbi.nlm.nih.gov/39738071/>39738071</a>
Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w. |
2024 Dec 30 |
degradation |
8 |
2 |
GH29,
GH95
|
| PUL0785 |
RNA-seq,
differential gene expression,
HPAEC-PAD,
SDS-PAGE,
para-hydroxybenzoic acid (PAHBAH) assay,
reducing-sugar assay,
Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE),
enzyme activity assay
|
fucoidan
|
Neorhodopirellula lusitana |
<a href=https://pubmed.ncbi.nlm.nih.gov/39738071/>39738071</a>
Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w. |
2024 Dec 30 |
degradation |
22 |
5 |
CE20,
CE20,
GH95,
CE7,
GH117,
GH168
|
