| PULID | Characterization Method(s) | Substrate | Organism | Publication | Publish Date | Type | Num Genes | Num CAZymes | CazyFamily |
|---|---|---|---|---|---|---|---|---|---|
| PUL0151 | sequence homology analysis, Northern Blot, RT-qPCR, electrophoretic mobility shift assay, clone and expression, gene deletion mutant and growth assay | alginate | Zobellia galactanivorans | <a href=https://pubmed.ncbi.nlm.nih.gov/30524390/>30524390</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/32585009/>32585009</a> Evolutionary Evidence of Algal Polysaccharide Degradation Acquisition by Pseudoalteromonas carrageenovora 9(T) to Adapt to Macroalgal Niches. Regulation of alginate catabolism involves a GntR family repressor in the marine flavobacterium Zobellia galactanivorans DsijT. Front Microbiol. 2018 Nov 22;9:2740. doi: 10.3389/fmicb.2018.02740. eCollection 2018. Nucleic Acids Res. 2020 Aug 20;48(14):7786-7800. doi: 10.1093/nar/gkaa533. |
2018,2020 Aug 20 | degradation | 12 | 2 | PL17_2, PL17, PL7 |
| PUL0216 | Western Blot, enzyme activity assay, RT-PCR, microarray | alginate | Sphingomonas sp. | <a href=https://pubmed.ncbi.nlm.nih.gov/24816607/>24816607</a> Alginate-dependent gene expression mechanism in Sphingomonas sp. strain A1. J Bacteriol. 2014 Jul;196(14):2691-700. doi: 10.1128/JB.01666-14. Epub 2014 May 9. |
2014 Jul | degradation | 10 | 3 | AA2, PL15_1, PL5, PL7 |
| PUL0235 | proteome fractionation, mass spectrometry, target decoy database analysis | alginate | Gramella forsetii | <a href=https://pubmed.ncbi.nlm.nih.gov/24522261/>24522261</a> Functional characterization of polysaccharide utilization loci in the marine Bacteroidetes 'Gramella forsetii' KT0803. ISME J. 2014 Jul;8(7):1492-502. doi: 10.1038/ismej.2014.4. Epub 2014 Feb 13. |
2014 Jul | degradation | 20 | 6 | PL17_2, PL17, PL6, PL6_1, PL7, PL7_5 |
| PUL0445 | recombinant protein expression, thin-layer chromatography, enzyme activity assay | alginate | Sphingomonas sp. | <a href=https://pubmed.ncbi.nlm.nih.gov/10913091/>10913091</a> Molecular identification of oligoalginate lyase of Sphingomonas sp. strain A1 as one of the enzymes required for complete depolymerization of alginate. J Bacteriol. 2000 Aug;182(16):4572-7. doi: 10.1128/JB.182.16.4572-4577.2000. |
2000 Aug | degradation | 8 | 2 | PL15_1, PL5, PL7 |
| PUL0655 | sequence homology analysis | alginate | Maribacter dokdonensis 62-1 | <a href=https://pubmed.ncbi.nlm.nih.gov/33912144/>33912144</a> CAZymes in Maribacter dokdonensis 62-1 From the Patagonian Shelf: Genomics and Physiology Compared to Related Flavobacteria and a Co-occurring Alteromonas Strain. Front Microbiol. 2021 Apr 12;12:628055. doi: 10.3389/fmicb.2021.628055. eCollection 2021. |
2021 | degradation | 10 | 2 | PL7 |
| PUL0691 | recombinant protein expression, qRT-PCR | alginate | Vibrio pelagius WXL662 | <a href=https://pubmed.ncbi.nlm.nih.gov/36409133/>36409133</a> Characterization of Multiple Alginate Lyases in a Highly Efficient Alginate-Degrading Vibrio Strain and Its Degradation Strategy. Appl Environ Microbiol. 2022 Dec 13;88(23):e0138922. doi: 10.1128/aem.01389-22. Epub 2022 Nov 21. |
2022 Dec 13 | degradation | 12 | 2 | CBM32, PL7_5, PL7, PL7 |
| PUL0715 | clone and expression, enzyme activity assay, ultra-performance liquid chromatography–mass spectrometry(UPLC-MS) | alginate | Wenyingzhuangia fucanilytica CZ1127 | <a href=https://pubmed.ncbi.nlm.nih.gov/37540808/>37540808</a> A repertoire of alginate lyases in the alginate polysaccharide utilization loci of marine bacterium Wenyingzhuangia fucanilytica: biochemical properties and action pattern. J Sci Food Agric. 2024 Jan 15;104(1):134-140. doi: 10.1002/jsfa.12898. Epub 2023 Aug 23. |
2024 Jan 15 | degradation | 12 | 4 | PL17_2, PL17, PL6, PL6_1, PL7 |
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