| PULID | Characterization Method(s) | Substrate | Organism | Publication | Publish Date | Type | Num Genes | Num CAZymes | CazyFamily |
|---|---|---|---|---|---|---|---|---|---|
| PUL0044 | qRT-PCR, enzyme activity assay | arabinoxylan | Bacteroides ovatus | <a href=https://pubmed.ncbi.nlm.nih.gov/26112186/>26112186</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/32266006/>32266006</a> Glycan complexity dictates microbial resource allocation in the large intestine. Multimodular fused acetyl-feruloyl esterases from soil and gut Bacteroidetes improve xylanase depolymerization of recalcitrant biomass. Nat Commun. 2015 Jun 26;6:7481. doi: 10.1038/ncomms8481. Biotechnol Biofuels. 2020 Mar 31;13:60. doi: 10.1186/s13068-020-01698-9. eCollection 2020. |
2015 Jun 26,2020 | degradation | 34 | 17 | CE20, CE20, CE6, CE1, GH10, GH115, GH3, GH30, GH30_8, GH31_4, GH43_10, CBM91, GH43_12, CBM91, GH43_29, CBM6, GH95, GH97, GH98, CBM35 |
| PUL0065 | bicinchoninic acid (BCA) reducing-sugar assay, enzyme product analysis, affinity gel electrophoresis, isothermal titration calorimetry (ITC) | xyloglucan | Bacteroides uniformis | <a href=https://pubmed.ncbi.nlm.nih.gov/31420336/>31420336</a> Adaptation of Syntenic Xyloglucan Utilization Loci of Human Gut Bacteroidetes to Polysaccharide Side Chain Diversity. Appl Environ Microbiol. 2019 Oct 1;85(20):e01491-19. doi: 10.1128/AEM.01491-19. Print 2019 Oct 15. |
2019 Oct 15 | degradation | 13 | 6 | GH2, GH3, GH31_4, GH43_12, CBM91, GH5_4, GH95 |
| PUL0066 | bicinchoninic acid (BCA) reducing-sugar assay, enzyme product analysis, affinity gel electrophoresis, isothermal titration calorimetry (ITC) | xyloglucan | Bacteroides fluxus | <a href=https://pubmed.ncbi.nlm.nih.gov/31420336/>31420336</a> Adaptation of Syntenic Xyloglucan Utilization Loci of Human Gut Bacteroidetes to Polysaccharide Side Chain Diversity. Appl Environ Microbiol. 2019 Oct 1;85(20):e01491-19. doi: 10.1128/AEM.01491-19. Print 2019 Oct 15. |
2019 Oct 15 | degradation | 13 | 6 | GH2, GH3, GH31_4, GH43_12, CBM91, GH5_4, GH95 |
| PUL0067 | bicinchoninic acid (BCA) reducing-sugar assay, enzyme product analysis, affinity gel electrophoresis, isothermal titration calorimetry (ITC) | xyloglucan | Dysgonomonas gadei | <a href=https://pubmed.ncbi.nlm.nih.gov/31420336/>31420336</a> Adaptation of Syntenic Xyloglucan Utilization Loci of Human Gut Bacteroidetes to Polysaccharide Side Chain Diversity. Appl Environ Microbiol. 2019 Oct 1;85(20):e01491-19. doi: 10.1128/AEM.01491-19. Print 2019 Oct 15. |
2019 Oct 15 | degradation | 11 | 6 | GH2, GH3, GH31_4, GH43_12, CBM91, GH5_4, GH95 |
| PUL0084 | label-free quantitative proteomics, functional enrichment analysis, enzyme activity assay | cellulose | Ruminiclostridium papyrosolvens | <a href=https://pubmed.ncbi.nlm.nih.gov/31338125/>31338125</a> Secretomic analyses of Ruminiclostridium papyrosolvens reveal its enzymatic basis for lignocellulose degradation. Biotechnol Biofuels. 2019 Jul 15;12:183. doi: 10.1186/s13068-019-1522-8. eCollection 2019. |
2019 | degradation | 12 | 12 | CE1, CBM6, GH10, CBM6, GH27, CBM6, GH30_8, CBM6, GH43_10, CBM91, CBM6, GH43_29, CBM6, GH59, CBM6, GH62, CBM6, GH62, CBM6, CE6, GH95, CBM32 |
| PUL0114 | recombinant protein expression, enzyme activity assay | arabinan | Ruminiclostridium cellulolyticum | <a href=https://pubmed.ncbi.nlm.nih.gov/31198441/>31198441</a> The xyl-doc gene cluster of Ruminiclostridium cellulolyticum encodes GH43- and GH62-alpha-l-arabinofuranosidases with complementary modes of action. Biotechnol Biofuels. 2019 Jun 10;12:144. doi: 10.1186/s13068-019-1483-y. eCollection 2019. |
2019 | degradation | 14 | 14 | CE1, CBM6, GH10, CBM6, GH146, CBM22, GH27, CBM6, GH2, CBM6, GH30_8, CBM6, GH43_10, CBM91, CBM6, GH43_16, CBM6, GH43_29, CBM6, GH59, CBM6, GH62, CBM6, GH62, CBM6, CE6, GH95, CBM32, CBM6 |
| PUL0189 | RNA-seq, RT-PCR, qPCR | pectin | Bacteroides xylanisolvens | <a href=https://pubmed.ncbi.nlm.nih.gov/26920945/>26920945</a> Unraveling the pectinolytic function of Bacteroides xylanisolvens using a RNA-seq approach and mutagenesis. BMC Genomics. 2016 Feb 27;17:147. doi: 10.1186/s12864-016-2472-1. |
2016 Feb 27 | degradation | 17 | 9 | CBM67, GH78, CBM67, GH78, GH33, CE19, GH140, GH28, GH43_18, GH92, GH95, PL1_2 |
| PUL0215 | qPCR, enzyme activity assay | xyloglucan | Cellvibrio japonicus | <a href=https://pubmed.ncbi.nlm.nih.gov/25171165/>25171165</a> A complex gene locus enables xyloglucan utilization in the model saprophyte Cellvibrio japonicus. Mol Microbiol. 2014 Oct;94(2):418-33. doi: 10.1111/mmi.12776. Epub 2014 Sep 17. |
2014 Oct | degradation | 4 | 3 | GH31_4, GH35, GH95 |
| PUL0224 | RT-PCR, qRT-PCR, ion trap liquid chromatography, mass spectrometry, target decoy database analysis, high-performance anion-exchange chromatography | cellulose | Ruminiclostridium cellulolyticum | <a href=https://pubmed.ncbi.nlm.nih.gov/23418511/>23418511</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/20013800/>20013800</a> A two-component system (XydS/R) controls the expression of genes encoding CBM6-containing proteins in response to straw in Clostridium cellulolyticum. Modulation of cellulosome composition in Clostridium cellulolyticum: adaptation to the polysaccharide environment revealed by proteomic and carbohydrate-active enzyme analyses. PLoS One. 2013;8(2):e56063. doi: 10.1371/journal.pone.0056063. Epub 2013 Feb 13. Proteomics. 2010 Feb;10(3):541-54. doi: 10.1002/pmic.200900311. |
2013,2010 Feb | degradation | 16 | 14 | CE1, CBM6, GH10, CBM6, GH146, CBM22, GH27, CBM6, GH2, CBM6, GH30_8, CBM6, GH43_10, CBM91, CBM6, GH43_16, CBM6, GH43_29, CBM6, GH59, CBM6, GH62, CBM6, GH62, CBM6, CE6, GH95, CBM32, CBM6 |
| PUL0245 | enzyme activity assay, gene deletion mutant and growth assay, Western Blot | fucose | Streptococcus pneumoniae | <a href=https://pubmed.ncbi.nlm.nih.gov/24333485/>24333485</a> Structural and functional analysis of fucose-processing enzymes from Streptococcus pneumoniae. J Mol Biol. 2014 Apr 3;426(7):1469-82. doi: 10.1016/j.jmb.2013.12.006. Epub 2013 Dec 12. |
2014 Apr 3 | degradation | 11 | 2 | GH95, GH98, CBM47, CBM47, CBM47 |
| PUL0262 | RNA-seq | xylan | Bacteroides cellulosilyticus | <a href=https://pubmed.ncbi.nlm.nih.gov/23976882/>23976882</a> Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20. |
2013 | degradation | 12 | 6 | CE1, CE6, GH95, GH10, GH5_21, GH8 |
| PUL0299 | gene trait matching exercise | human milk oligosaccharide | Bifidobacterium longum | <a href=https://pubmed.ncbi.nlm.nih.gov/29310579/>29310579</a> Gene-trait matching across the Bifidobacterium longum pan-genome reveals considerable diversity in carbohydrate catabolism among human infant strains. BMC Genomics. 2018 Jan 8;19(1):33. doi: 10.1186/s12864-017-4388-9. |
2018 Jan 8 | degradation | 13 | 2 | GH29, GH95 |
| PUL0342 | enzyme activity assay, gene deletion mutant and growth assay | xylan | Prevotella ruminicola | <a href=https://pubmed.ncbi.nlm.nih.gov/19304844/>19304844</a> Biochemical analysis of a beta-D-xylosidase and a bifunctional xylanase-ferulic acid esterase from a xylanolytic gene cluster in Prevotella ruminicola 23. J Bacteriol. 2009 May;191(10):3328-38. doi: 10.1128/JB.01628-08. Epub 2009 Mar 20. |
2009 May | degradation | 5 | 3 | GH10, CE1, GH3, GH95 |
| PUL0345 | qRT-PCR, enzyme activity assay | xylan | Bacteroides intestinalis | <a href=https://pubmed.ncbi.nlm.nih.gov/27681607/>27681607</a> Bacteroides intestinalis DSM 17393, a member of the human colonic microbiome, upregulates multiple endoxylanases during growth on xylan. Sci Rep. 2016 Sep 29;6:34360. doi: 10.1038/srep34360. |
2016 Sep 29 | degradation | 31 | 13 | CE1, CE20, CE20, CE6, GH95, GH10, GH10, GH43_12, CBM91, GH115, GH35, GH43_1, GH5_21, GH67, GH8 |
| PUL0368 | microarray, Western Blot | human milk oligosaccharide | Bifidobacterium longum subsp. infantis | <a href=https://pubmed.ncbi.nlm.nih.gov/19033196/>19033196</a> The genome sequence of Bifidobacterium longum subsp. infantis reveals adaptations for milk utilization within the infant microbiome. Proc Natl Acad Sci U S A. 2008 Dec 2;105(48):18964-9. doi: 10.1073/pnas.0809584105. Epub 2008 Nov 24. |
2008 Dec 2 | degradation | 30 | 5 | GH2, GH20, GH29, GH33, GH95 |
| PUL0479 | growth assay | pectin | Flavobacterium johnsoniae | <a href=https://pubmed.ncbi.nlm.nih.gov/19717629/>19717629</a> Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis. Appl Environ Microbiol. 2009 Nov;75(21):6864-75. doi: 10.1128/AEM.01495-09. Epub 2009 Aug 28. |
2009 Nov | degradation | 20 | 11 | GH2, GH27, GH28, GH43_19, GH43_34, GH51_2, GH89, GH92, GH95 |
| PUL0482 | growth assay | pectin | Flavobacterium johnsoniae | <a href=https://pubmed.ncbi.nlm.nih.gov/19717629/>19717629</a> Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis. Appl Environ Microbiol. 2009 Nov;75(21):6864-75. doi: 10.1128/AEM.01495-09. Epub 2009 Aug 28. |
2009 Nov | degradation | 27 | 21 | CBM67, GH78, CE19, CE20, CE8, GH106, GH127, GH137, GH139, GH140, GH142, GH143, GH2, GH28, GH43_18, GH78, GH95, PL1_2, PL29 |
| PUL0553 | RT-PCR, qPCR | xylan | Bacteroides xylanisolvens | <a href=https://pubmed.ncbi.nlm.nih.gov/27142817/>27142817</a> Xylan degradation by the human gut Bacteroides xylanisolvens XB1A(T) involves two distinct gene clusters that are linked at the transcriptional level. BMC Genomics. 2016 May 4;17:326. doi: 10.1186/s12864-016-2680-8. |
2016 May 4 | degradation | 22 | 13 | CE6, CE1, GH10, GH115, GH3, GH31_4, GH43_10, CBM91, GH43_12, CBM91, GH43_29, CBM6, GH5_21, GH95, GH97 |
| PUL0558 | gene deletion mutant and growth assay, growth assay, enzyme activity assay | pectin | Bacteroides thetaiotaomicron | <a href=https://pubmed.ncbi.nlm.nih.gov/28329766/>28329766</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/22205877/>22205877</a> Complex pectin metabolism by gut bacteria reveals novel catalytic functions. Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. Nature. 2017 Apr 6;544(7648):65-70. doi: 10.1038/nature21725. Epub 2017 Mar 22. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. |
2017 Apr 6,2011 Dec | degradation | 50 | 21 | CBM67, GH78, CBM67, GH78, GH33, CE19, CE20, GH105, GH106, GH127, GH137, GH2, CBM57, CBM97, GH138, GH139, GH140, GH141, GH143, GH142, GH2, GH28, GH43_18, GH78, GH95, PL1_2 |
| PUL0598 | liquid chromatography and mass spectrometry, differential gene expression | xylan | Clostridium cellulovorans 743B | <a href=https://pubmed.ncbi.nlm.nih.gov/26020016/>26020016</a> Elucidation of the recognition mechanisms for hemicellulose and pectin in Clostridium cellulovorans using intracellular quantitative proteome analysis. AMB Express. 2015 May 23;5:29. doi: 10.1186/s13568-015-0115-6. eCollection 2015. |
2015 | degradation | 4 | 1 | GH95 |
| PUL0609 | enzyme activity assay, clone and expression, liquid chromatography and mass spectrometry, thin-layer chromatography, MALDI-TOF/MS | human milk oligosaccharide | Roseburia inulinivorans DSM 16841 | <a href=https://pubmed.ncbi.nlm.nih.gov/32620774/>32620774</a> Butyrate producing colonic Clostridiales metabolise human milk oligosaccharides and cross feed on mucin via conserved pathways. Nat Commun. 2020 Jul 3;11(1):3285. doi: 10.1038/s41467-020-17075-x. |
2020 Jul 3 | degradation | 11 | 4 | GH112, GH136, CBM32, GH95 |
| PUL0625 | RNA-seq | xylan | Prevotella sp. PMUR | <a href=https://pubmed.ncbi.nlm.nih.gov/33113351/>33113351</a> Distinct Polysaccharide Utilization Determines Interspecies Competition between Intestinal Prevotella spp. Cell Host Microbe. 2020 Dec 9;28(6):838-852.e6. doi: 10.1016/j.chom.2020.09.012. Epub 2020 Oct 27. |
2020 Dec 9 | degradation | 18 | 10 | CE1, CE1, CE1, GH115, GH30_8, GH43_10, CBM91, GH43_12, CBM91, GH43_29, CBM6, GH95, GH97 |
| PUL0669 | clone, high-performance anion-exchange chromatography, enzymatic product analysis | xylan | Bacteroides eggerthii 1_2_48FAA | <a href=https://pubmed.ncbi.nlm.nih.gov/34480044/>34480044</a> Characterization of a novel multidomain CE15-GH8 enzyme encoded by a polysaccharide utilization locus in the human gut bacterium Bacteroides eggerthii. Sci Rep. 2021 Sep 3;11(1):17662. doi: 10.1038/s41598-021-96659-z. |
2021 Sep 3 | degradation | 26 | 15 | CE1, CE15, GH8, CE20, CE20, CE6, GH10, GH115, GH31_4, GH35, GH43_1, GH43_10, CBM91, GH43_12, CBM91, GH5_21, GH67, GH95, GH97 |
| PUL0673 | NMR, substrate binding assay, liquid chromatography and mass spectrometry | human milk oligosaccharide | Bifidobacterium pseudocatenulatum DSM20438 | <a href=https://pubmed.ncbi.nlm.nih.gov/34757822/>34757822</a> Fucosylated Human Milk Oligosaccharide Foraging within the Species Bifidobacterium pseudocatenulatum Is Driven by Glycosyl Hydrolase Content and Specificity. Appl Environ Microbiol. 2022 Jan 25;88(2):e0170721. doi: 10.1128/AEM.01707-21. Epub 2021 Nov 10. |
2022 Jan 25 | degradation | 8 | 1 | GH95 |
| PUL0688 | clone and expression, crystallization, recombinant protein expression, thin-layer chromatography | galactooligosaccharide | Bacteroides thetaiotaomicron VPI-5482 | <a href=https://pubmed.ncbi.nlm.nih.gov/34149636/>34149636</a> Analysis of Two SusE-Like Enzymes From Bacteroides thetaiotaomicron Reveals a Potential Degradative Capacity for This Protein Family. Front Microbiol. 2021 Jun 4;12:645765. doi: 10.3389/fmicb.2021.645765. eCollection 2021. |
2021 | degradation | 5 | 1 | GH95 |
| PUL0702 | enzyme activity assay, gene deletion mutant and growth assay | xyloglucan | Xanthomonas citri pv. citri str. 306 | <a href=https://pubmed.ncbi.nlm.nih.gov/34193873/>34193873</a> Xyloglucan processing machinery in Xanthomonas pathogens and its role in the transcriptional activation of virulence factors. Nat Commun. 2021 Jun 30;12(1):4049. doi: 10.1038/s41467-021-24277-4. |
2021 Jun 30 | degradation | 8 | 5 | CE20, CE20, GH31_4, GH35, GH74, GH95 |
| PUL0705 | fluorophore-assisted carbohydrate electrophoresis (FACE), dinitrosalicylic acid-assay (DNS-assay), HPLC, clone and expression | xylan | Flavimarina sp. Hel_I_48 | <a href=https://pubmed.ncbi.nlm.nih.gov/37121608/>37121608</a> Marine Bacteroidetes enzymatically digest xylans from terrestrial plants. Environ Microbiol. 2023 Sep;25(9):1713-1727. doi: 10.1111/1462-2920.16390. Epub 2023 Apr 30. |
2023 Sep | degradation | 14 | 8 | CE6, CE1, CE1, GH10, GH43_10, CBM91, GH43_12, CBM91, GH8, GH95, GH97 |
| PUL0727 | SDS-PAGE, NMR, enzyme activity assay, size-exclusion chromatography (SEC) | fucoidan | Wenyingzhuangia fucanilytica strain CZ1127 | <a href=https://pubmed.ncbi.nlm.nih.gov/38203394/>38203394</a> The Discovery of the Fucoidan-Active Endo-1-->4-alpha-L-Fucanase of the GH168 Family, Which Produces Fucoidan Derivatives with Regular Sulfation and Anticoagulant Activity. Int J Mol Sci. 2023 Dec 22;25(1):218. doi: 10.3390/ijms25010218. |
2023 Dec 22 | degradation | 30 | 16 | GH107, GH117, GH141, GH168, GH29, GH43_2, GH95 |
| PUL0764 | RNA-seq, qRT-PCR, gas chromatography, mass spectrometry, thin-layer chromatography | Hemicellulose | Segatella copri DSM 18205 | <a href=https://pubmed.ncbi.nlm.nih.gov/39636128/>39636128</a> Transcriptional delineation of polysaccharide utilization loci in the human gut commensal Segatella copri DSM18205 and co-culture with exemplar Bacteroides species on dietary plant glycans. Appl Environ Microbiol. 2025 Jan 31;91(1):e0175924. doi: 10.1128/aem.01759-24. Epub 2024 Dec 5. |
2025 Jan 31 | degradation | 4 | 4 | GH43_10, CBM91, GH43_29, CBM6, GH43_29, CBM6, GH43_10, CBM91, GH95 |
| PUL0767 | RNA-seq, qRT-PCR, gas chromatography, mass spectrometry, thin-layer chromatography | pectin | Segatella copri DSM 18205 | <a href=https://pubmed.ncbi.nlm.nih.gov/39636128/>39636128</a> Transcriptional delineation of polysaccharide utilization loci in the human gut commensal Segatella copri DSM18205 and co-culture with exemplar Bacteroides species on dietary plant glycans. Appl Environ Microbiol. 2025 Jan 31;91(1):e0175924. doi: 10.1128/aem.01759-24. Epub 2024 Dec 5. |
2025 Jan 31 | degradation | 15 | 6 | CE8, GH28, GH28, GH105, GH43_10, CBM91, GH95, PL1_2 |
| PUL0774 | RNA-seq, differential gene expression, HPAEC-PAD, SDS-PAGE, para-hydroxybenzoic acid (PAHBAH) assay, reducing-sugar assay, Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE), enzyme activity assay | fucoidan | Rhodopirellula sp. SWK7 | <a href=https://pubmed.ncbi.nlm.nih.gov/39738071/>39738071</a> Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w. |
2024 Dec 30 | degradation | 23 | 8 | GH117, GH141, GH168, GH29, GH95 |
| PUL0776 | RNA-seq, differential gene expression, HPAEC-PAD, SDS-PAGE, para-hydroxybenzoic acid (PAHBAH) assay, reducing-sugar assay, Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE), enzyme activity assay | fucoidan | Rhodopirellula sp. SWK7 | <a href=https://pubmed.ncbi.nlm.nih.gov/39738071/>39738071</a> Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w. |
2024 Dec 30 | degradation | 32 | 10 | CE19, CBM51, CE20, GH115, GH116, GH117, GH117, GH29, GH95 |
| PUL0778 | RNA-seq, differential gene expression, HPAEC-PAD, SDS-PAGE, para-hydroxybenzoic acid (PAHBAH) assay, reducing-sugar assay, Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE), enzyme activity assay | fucoidan | Neorhodopirellula lusitana | <a href=https://pubmed.ncbi.nlm.nih.gov/39738071/>39738071</a> Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w. |
2024 Dec 30 | degradation | 18 | 4 | GH172, GH29, GH3, GH95 |
| PUL0780 | RNA-seq, differential gene expression, HPAEC-PAD, SDS-PAGE, para-hydroxybenzoic acid (PAHBAH) assay, reducing-sugar assay, Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE), enzyme activity assay | fucoidan | Neorhodopirellula lusitana | <a href=https://pubmed.ncbi.nlm.nih.gov/39738071/>39738071</a> Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w. |
2024 Dec 30 | degradation | 24 | 8 | CBM32, CE12, CE6, GH141, GH29, GH95 |
| PUL0781 | RNA-seq, differential gene expression, HPAEC-PAD, SDS-PAGE, para-hydroxybenzoic acid (PAHBAH) assay, reducing-sugar assay, Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE), enzyme activity assay | fucoidan | Neorhodopirellula lusitana | <a href=https://pubmed.ncbi.nlm.nih.gov/39738071/>39738071</a> Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w. |
2024 Dec 30 | degradation | 17 | 4 | GH116, GH29, GH95 |
| PUL0783 | RNA-seq, differential gene expression, HPAEC-PAD, SDS-PAGE, para-hydroxybenzoic acid (PAHBAH) assay, reducing-sugar assay, Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE), enzyme activity assay | fucoidan | Neorhodopirellula lusitana | <a href=https://pubmed.ncbi.nlm.nih.gov/39738071/>39738071</a> Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w. |
2024 Dec 30 | degradation | 20 | 6 | CBM51, GH115, GH172, GH28, GH29, GH95 |
| PUL0784 | RNA-seq, differential gene expression, HPAEC-PAD, SDS-PAGE, para-hydroxybenzoic acid (PAHBAH) assay, reducing-sugar assay, Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE), enzyme activity assay | fucoidan | Neorhodopirellula lusitana | <a href=https://pubmed.ncbi.nlm.nih.gov/39738071/>39738071</a> Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w. |
2024 Dec 30 | degradation | 8 | 2 | GH29, GH95 |
| PUL0785 | RNA-seq, differential gene expression, HPAEC-PAD, SDS-PAGE, para-hydroxybenzoic acid (PAHBAH) assay, reducing-sugar assay, Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE), enzyme activity assay | fucoidan | Neorhodopirellula lusitana | <a href=https://pubmed.ncbi.nlm.nih.gov/39738071/>39738071</a> Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w. |
2024 Dec 30 | degradation | 22 | 5 | CE20, CE20, GH95, CE7, GH117, GH168 |
| PUL0786 | RNA-seq, reducing-sugar assay, growth assay, high performance gel permeation chromatography, gas chromatography, RNA-seq, differential gene expression | pectic polysaccharide | Bacteroides ovatus strain ATCC 8483 | <a href=https://pubmed.ncbi.nlm.nih.gov/38890895/>38890895</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/39892338/>39892338</a> The Utilization by Bacteroides spp. of a Purified Polysaccharide from Fuzhuan Brick Tea. In vitro fermentation of a purified fraction of polysaccharides from the root of Brassica rapa L. by human gut microbiota and its interaction with Bacteroides ovatus. Foods. 2024 May 26;13(11):1666. doi: 10.3390/foods13111666. Food Chem. 2025 May 1;473:143109. doi: 10.1016/j.foodchem.2025.143109. Epub 2025 Jan 27. |
2024 May 26,2025 May 1 | degradation | 26 | 14 | CBM67, GH78, CBM67, GH78, GH33, CE19, GH105, GH130_2, GH140, GH143, GH142, GH163, GH18, GH28, GH43_18, GH92, GH95, PL1_2 |
| PUL0795 | RNA-seq, recombinant protein expression, growth assay | xyloglucan | Flavobacterium johnsoniae UW101 | <a href=https://pubmed.ncbi.nlm.nih.gov/39913342/>39913342</a> Metabolism of hemicelluloses by root-associated Bacteroidota species. ISME J. 2025 Jan 2;19(1):wraf022. doi: 10.1093/ismejo/wraf022. |
2025 Jan 2 | degradation | 12 | 8 | CE20, CE20, GH2, GH3, GH31_3, GH39, GH5_4, GH95, GH97 |
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