| PULID | Characterization Method(s) | Substrate | Organism | Publication | Publish Date | Type | Num Genes | Num CAZymes | CazyFamily |
|---|---|---|---|---|---|---|---|---|---|
| PUL0197 | gene deletion mutant and growth assay | starch | Streptococcus mutans | <a href=https://pubmed.ncbi.nlm.nih.gov/17233733/>17233733</a> Overlapping substrate specificity for sucrose and maltose of two binding protein-dependent sugar uptake systems in Streptococcus mutans. FEMS Microbiol Lett. 2007 Jan;266(2):218-23. doi: 10.1111/j.1574-6968.2006.00522.x. |
2007 Jan | degradation | 7 | 2 | GH77, GT35 |
| PUL0275 | RT-qPCR | starch | Bifidobacterium animalis subsp. lactis | <a href=https://pubmed.ncbi.nlm.nih.gov/23663691/>23663691</a> Transcriptional analysis of oligosaccharide utilization by Bifidobacterium lactis Bl-04. BMC Genomics. 2013 May 10;14:312. doi: 10.1186/1471-2164-14-312. |
2013 May 10 | degradation | 8 | 3 | GH13_30, GH13_44, GH77 |
| PUL0454 | SDS-PAGE, protein fingerprinting (MALDI-TOF PMF) | alpha-glucan | Actinoplanes sp. SE50/110 | <a href=https://pubmed.ncbi.nlm.nih.gov/22944206/>22944206</a> The cytosolic and extracellular proteomes of Actinoplanes sp. SE50/110 led to the identification of gene products involved in acarbose metabolism. J Biotechnol. 2013 Aug 20;167(2):178-89. doi: 10.1016/j.jbiotec.2012.08.011. Epub 2012 Aug 31. |
2013 Aug 20 | biosynthesis | 22 | 6 | GH13_13, CBM41, CBM41, GH13_2, CBM20, GH77, GT5 |
| PUL0519 | gene deletion mutant and growth assay | starch | Streptococcus pneumoniae | <a href=https://pubmed.ncbi.nlm.nih.gov/8244973/>8244973</a> Characterization of the Streptococcus pneumoniae maltosaccharide regulator MalR, a member of the LacI-GalR family of repressors displaying distinctive genetic features. J Biol Chem. 1993 Dec 5;268(34):25402-8. |
1993 Dec 5 | degradation | 7 | 2 | GH77, GT35 |
| PUL0589 | Western Blot, enzyme activity assay, thin-layer chromatography | starch | Streptococcus mutans | <a href=https://pubmed.ncbi.nlm.nih.gov/23930155/>23930155</a> The malQ gene is essential for starch metabolism in Streptococcus mutans. J Oral Microbiol. 2013 Aug 6;5. doi: 10.3402/jom.v5i0.21285. Print 2013. |
2013 | degradation | 3 | 2 | GH77, GT35 |
| PUL0748 | Reducing End Assay, HPAEC-PAD | glycogen | Pontibacter sp. SGAir0037 | <a href=https://pubmed.ncbi.nlm.nih.gov/38930854/>38930854</a> Characterization of Two Glycoside Hydrolases of Family GH13 and GH57, Present in a Polysaccharide Utilization Locus (PUL) of Pontibacter sp. SGAir0037. Molecules. 2024 Jun 12;29(12):2788. doi: 10.3390/molecules29122788. |
2024 Jun 12 | degradation | 14 | 10 | CBM48, GH13_10, CBM48, GH13_9, GH13, GH13_16, GH13_26, GH13_3, GH57, GH77, GH97 |
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