| PULID | Characterization Method(s) | Substrate | Organism | Publication | Publish Date | Type | Num Genes | Num CAZymes | CazyFamily |
|---|---|---|---|---|---|---|---|---|---|
| PUL0083 | label-free quantitative proteomics, functional enrichment analysis, enzyme activity assay | cellulose | Ruminiclostridium papyrosolvens | 31338125 Secretomic analyses of Ruminiclostridium papyrosolvens reveal its enzymatic basis for lignocellulose degradation. Biotechnol Biofuels. 2019 Jul 15;12:183. doi: 10.1186/s13068-019-1522-8. eCollection 2019. |
2019 | degradation | 12 | 11 | CBM3, cohesin, cohesin, cohesin, cohesin, cohesin, cohesin, CBM4, GH9, GH48, GH5_1, GH5_17, GH5_7, GH8, GH9, GH9, CBM3 |
| PUL0164 | mass spectrometry, sequence homology analysis, differential gene expression | beta-mannan | Leeuwenhoekiella sp. MAR_2009_132 | 30246424 Alpha- and beta-mannan utilization by marine Bacteroidetes. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16. |
2018 Nov | degradation | 19 | 12 | CBM8, CE2, CE20, GH130_1, GH26, GH27, GH3, GH5_2, GH5_7, GH9 |
| PUL0175 | enzyme activity assay | galactomannan | Cellvibrio mixtus | 16842369 Galactomannan hydrolysis and mannose metabolism in Cellvibrio mixtus. FEMS Microbiol Lett. 2006 Aug;261(1):123-32. doi: 10.1111/j.1574-6968.2006.00342.x. |
2006 Aug | degradation | 4 | 3 | GH130_1, GH27, GH5_7 |
| PUL0222 | fosmid library screen | cellulose | uncultured bacterium Contig196 | 24223817 Metagenomic insights into the carbohydrate-active enzymes carried by the microorganisms adhering to solid digesta in the rumen of cows. PLoS One. 2013 Nov 5;8(11):e78507. doi: 10.1371/journal.pone.0078507. eCollection 2013. |
2013 | degradation | 7 | 3 | GH26, GH5_4, GH5_7 |
| PUL0239 | fosmid library screen, sequence homology analysis | cellulose | Prevotella sp. Sc00026 | 24448980 Analysis of the bovine rumen microbiome reveals a diversity of Sus-like polysaccharide utilization loci from the bacterial phylum Bacteroidetes. J Ind Microbiol Biotechnol. 2014 Mar;41(3):601-6. doi: 10.1007/s10295-013-1395-y. Epub 2014 Jan 22. |
2014 Mar | degradation | 16 | 9 | CE20, CE7, GH130_1, GH26, GH26, GH5_4, GH3, GH36, GH5_7 |
| PUL0332 | fosmid library screen, enzyme activity assay, thin-layer chromatography | beta-glucan | uncultured bacterium | 28091525 A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 19 | 8 | CE7, GH127, GH2, GH5_2, GH5_7, GH94, GH97 |
| PUL0488 | growth assay | beta-mannan | Flavobacterium johnsoniae | 19717629 Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis. Appl Environ Microbiol. 2009 Nov;75(21):6864-75. doi: 10.1128/AEM.01495-09. Epub 2009 Aug 28. |
2009 Nov | degradation | 14 | 7 | GH130_1, GH26, GH27, GH5_2, GH5_7, GH97 |
| PUL0538 | RNA-seq | galactomannan | Bacteroides cellulosilyticus | 23976882 Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20. |
2013 | degradation | 15 | 7 | CE7, GH130_1, GH26, GH26, GH26, GH3, GH5_2, GH5_7 |
| PUL0662 | thin-layer chromatography, liquid chromatography and mass spectrometry, qPCR, clone and expression | beta-mannan | Phocaeicola dorei DSM 17855 | 34339781 BdPUL12 depolymerizes beta-mannan-like glycans into mannooligosaccharides and mannose, which serve as carbon sources for Bacteroides dorei and gut probiotics. Int J Biol Macromol. 2021 Sep 30;187:664-674. doi: 10.1016/j.ijbiomac.2021.07.172. Epub 2021 Jul 31. |
2021 Sep 30 | degradation | 8 | 4 | CE7, GH130_1, GH26, GH5_7 |
| PUL0760 | RNA-seq, qRT-PCR, gas chromatography, mass spectrometry, thin-layer chromatography | Hemicellulose | Segatella copri DSM 18205 | 39636128 Transcriptional delineation of polysaccharide utilization loci in the human gut commensal Segatella copri DSM18205 and co-culture with exemplar Bacteroides species on dietary plant glycans. Appl Environ Microbiol. 2025 Jan 31;91(1):e0175924. doi: 10.1128/aem.01759-24. Epub 2024 Dec 5. |
2025 Jan 31 | degradation | 7 | 3 | GH5_4, GH5_7 |
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