| PULID | Characterization Method(s) | Substrate | Organism | Publication | Publish Date | Type | Num Genes | Num CAZymes | CazyFamily |
|---|---|---|---|---|---|---|---|---|---|
| PUL0050 | fosmid library screen | cellulose | feces metagenome | 29601586 Two new gene clusters involved in the degradation of plant cell wall from the fecal microbiota of Tunisian dromedary. PLoS One. 2018 Mar 30;13(3):e0194621. doi: 10.1371/journal.pone.0194621. eCollection 2018. |
2018 | degradation | 20 | 6 | GH130_1, GH26, GH3, GH5_4, GH94 |
| PUL0063 | bicinchoninic acid (BCA) reducing-sugar assay, enzyme product analysis, affinity gel electrophoresis, isothermal titration calorimetry (ITC) | xyloglucan | Bacteroides ovatus | 31420336 Adaptation of Syntenic Xyloglucan Utilization Loci of Human Gut Bacteroidetes to Polysaccharide Side Chain Diversity. Appl Environ Microbiol. 2019 Oct 1;85(20):e01491-19. doi: 10.1128/AEM.01491-19. Print 2019 Oct 15. |
2019 Oct 15 | degradation | 16 | 8 | GH2, GH3, GH31_4, GH43_12, CBM91, GH5_4, GH9 |
| PUL0064 | bicinchoninic acid (BCA) reducing-sugar assay, enzyme product analysis, affinity gel electrophoresis, isothermal titration calorimetry (ITC) | xyloglucan | Bacteroides cellulosilyticus | 31420336 Adaptation of Syntenic Xyloglucan Utilization Loci of Human Gut Bacteroidetes to Polysaccharide Side Chain Diversity. Appl Environ Microbiol. 2019 Oct 1;85(20):e01491-19. doi: 10.1128/AEM.01491-19. Print 2019 Oct 15. |
2019 Oct 15 | degradation | 12 | 4 | GH2, GH3, GH31_4, GH5_4 |
| PUL0065 | bicinchoninic acid (BCA) reducing-sugar assay, enzyme product analysis, affinity gel electrophoresis, isothermal titration calorimetry (ITC) | xyloglucan | Bacteroides uniformis | 31420336 Adaptation of Syntenic Xyloglucan Utilization Loci of Human Gut Bacteroidetes to Polysaccharide Side Chain Diversity. Appl Environ Microbiol. 2019 Oct 1;85(20):e01491-19. doi: 10.1128/AEM.01491-19. Print 2019 Oct 15. |
2019 Oct 15 | degradation | 13 | 6 | GH2, GH3, GH31_4, GH43_12, CBM91, GH5_4, GH95 |
| PUL0066 | bicinchoninic acid (BCA) reducing-sugar assay, enzyme product analysis, affinity gel electrophoresis, isothermal titration calorimetry (ITC) | xyloglucan | Bacteroides fluxus | 31420336 Adaptation of Syntenic Xyloglucan Utilization Loci of Human Gut Bacteroidetes to Polysaccharide Side Chain Diversity. Appl Environ Microbiol. 2019 Oct 1;85(20):e01491-19. doi: 10.1128/AEM.01491-19. Print 2019 Oct 15. |
2019 Oct 15 | degradation | 13 | 6 | GH2, GH3, GH31_4, GH43_12, CBM91, GH5_4, GH95 |
| PUL0067 | bicinchoninic acid (BCA) reducing-sugar assay, enzyme product analysis, affinity gel electrophoresis, isothermal titration calorimetry (ITC) | xyloglucan | Dysgonomonas gadei | 31420336 Adaptation of Syntenic Xyloglucan Utilization Loci of Human Gut Bacteroidetes to Polysaccharide Side Chain Diversity. Appl Environ Microbiol. 2019 Oct 1;85(20):e01491-19. doi: 10.1128/AEM.01491-19. Print 2019 Oct 15. |
2019 Oct 15 | degradation | 11 | 6 | GH2, GH3, GH31_4, GH43_12, CBM91, GH5_4, GH95 |
| PUL0089 | label-free quantitative proteomics, functional enrichment analysis, enzyme activity assay | cellulose | Ruminiclostridium papyrosolvens | 31338125 Secretomic analyses of Ruminiclostridium papyrosolvens reveal its enzymatic basis for lignocellulose degradation. Biotechnol Biofuels. 2019 Jul 15;12:183. doi: 10.1186/s13068-019-1522-8. eCollection 2019. |
2019 | degradation | 2 | 1 | GH5_4 |
| PUL0129 | enzyme activity assay | beta-mannan | gut metagenome | 30356154 Interspecies cross-feeding orchestrates carbon degradation in the rumen ecosystem. Nat Microbiol. 2018 Nov;3(11):1274-1284. doi: 10.1038/s41564-018-0225-4. Epub 2018 Oct 24. |
2018 Nov | degradation | 12 | 6 | CE7, GH130_1, GH26, GH5_4 |
| PUL0132 | enzyme activity assay, microarray | beta-glucan | Zobellia galactanivorans | 30341165 The laterally acquired GH5 ZgEngA(GH5_4) from the marine bacterium Zobellia galactanivorans is dedicated to hemicellulose hydrolysis. Gene Expression Analysis of Zobellia galactanivorans during the Degradation of Algal Polysaccharides Reveals both Substrate-Specific and Shared Transcriptome-Wide Responses. Biochem J. 2018 Nov 28;475(22):3609-3628. doi: 10.1042/BCJ20180486. Front Microbiol. 2017 Sep 21;8:1808. doi: 10.3389/fmicb.2017.01808. eCollection 2017. |
2018 Nov 28,2017 | degradation | 8 | 2 | CBM4, GH5_4 |
| PUL0221 | fosmid library screen | cellulose | uncultured bacterium Contig1529 | 24223817 Metagenomic insights into the carbohydrate-active enzymes carried by the microorganisms adhering to solid digesta in the rumen of cows. PLoS One. 2013 Nov 5;8(11):e78507. doi: 10.1371/journal.pone.0078507. eCollection 2013. |
2013 | degradation | 10 | 4 | GH105, GH3, GH35, GH5_4 |
| PUL0222 | fosmid library screen | cellulose | uncultured bacterium Contig196 | 24223817 Metagenomic insights into the carbohydrate-active enzymes carried by the microorganisms adhering to solid digesta in the rumen of cows. PLoS One. 2013 Nov 5;8(11):e78507. doi: 10.1371/journal.pone.0078507. eCollection 2013. |
2013 | degradation | 7 | 3 | GH26, GH5_4, GH5_7 |
| PUL0239 | fosmid library screen, sequence homology analysis | cellulose | Prevotella sp. Sc00026 | 24448980 Analysis of the bovine rumen microbiome reveals a diversity of Sus-like polysaccharide utilization loci from the bacterial phylum Bacteroidetes. J Ind Microbiol Biotechnol. 2014 Mar;41(3):601-6. doi: 10.1007/s10295-013-1395-y. Epub 2014 Jan 22. |
2014 Mar | degradation | 16 | 9 | CE20, CE7, GH130_1, GH26, GH26, GH5_4, GH3, GH36, GH5_7 |
| PUL0241 | fosmid library screen, sequence homology analysis | cellulose | Prevotella sp. Sc00033 | 24448980 Analysis of the bovine rumen microbiome reveals a diversity of Sus-like polysaccharide utilization loci from the bacterial phylum Bacteroidetes. J Ind Microbiol Biotechnol. 2014 Mar;41(3):601-6. doi: 10.1007/s10295-013-1395-y. Epub 2014 Jan 22. |
2014 Mar | degradation | 6 | 2 | GH36, GH5_4 |
| PUL0242 | fosmid library screen, sequence homology analysis | cellulose | Prevotella sp. Sc00044 | 24448980 Analysis of the bovine rumen microbiome reveals a diversity of Sus-like polysaccharide utilization loci from the bacterial phylum Bacteroidetes. J Ind Microbiol Biotechnol. 2014 Mar;41(3):601-6. doi: 10.1007/s10295-013-1395-y. Epub 2014 Jan 22. |
2014 Mar | degradation | 9 | 3 | GH26, GH31_3, GH5_4 |
| PUL0527 | microarray, qPCR | xyloglucan | Bacteroides ovatus | 22205877 Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. |
2011 Dec | degradation | 16 | 8 | GH2, GH3, GH31_4, GH43_12, CBM91, GH5_4, GH9 |
| PUL0680 | gene deletion mutant and growth assay, clone and expression, qRT-PCR, high-performance anion-exchange chromatography, crystallization, recombinant protein expression | xyloglucan | Bacteroides uniformis ATCC 8492 | 34995484 Polysaccharide utilization loci in Bacteroides determine population fitness and community-level interactions. Cell Surface Xyloglucan Recognition and Hydrolysis by the Human Gut Commensal Bacteroides uniformis. Cell Host Microbe. 2022 Feb 9;30(2):200-215.e12. doi: 10.1016/j.chom.2021.12.006. Epub 2022 Jan 6. Appl Environ Microbiol. 2022 Jan 11;88(1):e0156621. doi: 10.1128/AEM.01566-21. Epub 2021 Nov 3. |
2022 Feb 9,2022 Jan 11 | degradation | 15 | 10 | CE20, GH43_16, CBM6, GH2, GH29, GH2, GH2, GH31_4, GH42, GH43_33, GH5_4, GH97 |
| PUL0750 | RNA-seq, BCA assay, pNP glycoside assay, HPAEC-PAD, qRT-PCR, gas chromatography, mass spectrometry, thin-layer chromatography | beta-glucan | Segatella copri DSM 18205 | 39122003 The molecular basis of cereal mixed-linkage beta-glucan utilization by the human gut bacterium Segatella copri. Transcriptional delineation of polysaccharide utilization loci in the human gut commensal Segatella copri DSM18205 and co-culture with exemplar Bacteroides species on dietary plant glycans. J Biol Chem. 2024 Sep;300(9):107625. doi: 10.1016/j.jbc.2024.107625. Epub 2024 Aug 8. Appl Environ Microbiol. 2025 Jan 31;91(1):e0175924. doi: 10.1128/aem.01759-24. Epub 2024 Dec 5. |
2024 Sep,2025 Jan 31 | degradation | 11 | 3 | GH3, GH5_4, GH94 |
| PUL0760 | RNA-seq, qRT-PCR, gas chromatography, mass spectrometry, thin-layer chromatography | Hemicellulose | Segatella copri DSM 18205 | 39636128 Transcriptional delineation of polysaccharide utilization loci in the human gut commensal Segatella copri DSM18205 and co-culture with exemplar Bacteroides species on dietary plant glycans. Appl Environ Microbiol. 2025 Jan 31;91(1):e0175924. doi: 10.1128/aem.01759-24. Epub 2024 Dec 5. |
2025 Jan 31 | degradation | 7 | 3 | GH5_4, GH5_7 |
| PUL0795 | RNA-seq, recombinant protein expression, growth assay | xyloglucan | Flavobacterium johnsoniae UW101 | 39913342 Metabolism of hemicelluloses by root-associated Bacteroidota species. ISME J. 2025 Jan 2;19(1):wraf022. doi: 10.1093/ismejo/wraf022. |
2025 Jan 2 | degradation | 12 | 8 | CE20, CE20, GH2, GH3, GH31_3, GH39, GH5_4, GH95, GH97 |
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