Browse dbCAN-PUL Entries

PULID	Characterization Method(s)	Substrate	Organism	Publication	Publish Date	Type	Num Genes	Num CAZymes	CazyFamily
PUL0007	sequence homology analysis	galactan	Leuconostoc gelidum	<a href=https://pubmed.ncbi.nlm.nih.gov/27274361/>27274361</a> Complete genome sequence of Leuconostoc gelidum subsp. gasicomitatum KG16-1, isolated from vacuum-packaged vegetable sausages. Stand Genomic Sci. 2016 Jun 7;11:40. doi: 10.1186/s40793-016-0164-8. eCollection 2016.	2016	degradation	8	2	GH42, GH53
PUL0209	enzyme activity assay, gene deletion mutant and growth assay	galactan	Dickeya dadantii	<a href=https://pubmed.ncbi.nlm.nih.gov/17644603/>17644603</a> Characterization of the Erwinia chrysanthemi Gan locus, involved in galactan catabolism. J Bacteriol. 2007 Oct;189(19):7053-61. doi: 10.1128/JB.00845-07. Epub 2007 Jul 20.	2007 Oct	degradation	9	2	GH42, GH53
PUL0282	enzyme activity assay	galactan	Geobacillus stearothermophilus	<a href=https://pubmed.ncbi.nlm.nih.gov/23216604/>23216604</a> Functional characterization of the galactan utilization system of Geobacillus stearothermophilus. FEBS J. 2013 Feb;280(3):950-64. doi: 10.1111/febs.12089. Epub 2013 Jan 7.	2013 Feb	degradation	7	2	CBM61, GH53, CBM61, GH42
PUL0283	microarray, high-performance anion-exchange chromatography, liquid chromatography and mass spectrometry	galactooligosaccharide	Bifidobacterium breve	<a href=https://pubmed.ncbi.nlm.nih.gov/23199239/>23199239</a> Transcriptional and functional characterization of genetic elements involved in galacto-oligosaccharide utilization by Bifidobacterium breve UCC2003. Microb Biotechnol. 2013 Jan;6(1):67-79. doi: 10.1111/1751-7915.12011. Epub 2012 Dec 2.	2013 Jan	degradation	6	2	GH42, GH53, CBM61
PUL0297	gene trait matching exercise	galactan	Bifidobacterium longum	<a href=https://pubmed.ncbi.nlm.nih.gov/29310579/>29310579</a> Gene-trait matching across the Bifidobacterium longum pan-genome reveals considerable diversity in carbohydrate catabolism among human infant strains. BMC Genomics. 2018 Jan 8;19(1):33. doi: 10.1186/s12864-017-4388-9.	2018 Jan 8	degradation	9	2	GH42, GH53, CBM61
PUL0304	RT-qPCR, isothermal titration calorimetry (ITC), enzyme activity assay, gene deletion mutant and growth assay	galactan	Bacteroides thetaiotaomicron	<a href=https://pubmed.ncbi.nlm.nih.gov/29255254/>29255254</a> Dietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides. Nat Microbiol. 2018 Feb;3(2):210-219. doi: 10.1038/s41564-017-0079-1. Epub 2017 Dec 18.	2018 Feb	degradation	7	2	GH2, GH53
PUL0306	RT-qPCR, isothermal titration calorimetry (ITC), enzyme activity assay, gene deletion mutant and growth assay	pectin	Bacteroides ovatus	<a href=https://pubmed.ncbi.nlm.nih.gov/29255254/>29255254</a> Dietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides. Nat Microbiol. 2018 Feb;3(2):210-219. doi: 10.1038/s41564-017-0079-1. Epub 2017 Dec 18.	2018 Feb	degradation	7	3	GH147, GH2, GH53
PUL0323	fitness contribution assay, insertion sequencing	galactan	Bacillus subtilis subsp. subtilis	<a href=https://pubmed.ncbi.nlm.nih.gov/28617843/>28617843</a> Characterization of the regulation of a plant polysaccharide utilization operon and its role in biofilm formation in Bacillus subtilis. PLoS One. 2017 Jun 15;12(6):e0179761. doi: 10.1371/journal.pone.0179761. eCollection 2017.	2017	degradation	6	2	GH42, GH53
PUL0427	microarray	pectin	Bacteroides ovatus	<a href=https://pubmed.ncbi.nlm.nih.gov/22205877/>22205877</a> Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.	2011 Dec	degradation	6	2	GH147, GH53
PUL0534	RNA-seq	pectin	Bacteroides cellulosilyticus	<a href=https://pubmed.ncbi.nlm.nih.gov/23976882/>23976882</a> Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20.	2013	degradation	9	5	GH13_10, GH133, GH147, GH2, GH53
PUL0566	microarray, qPCR	pectin	Bacteroides ovatus	<a href=https://pubmed.ncbi.nlm.nih.gov/22205877/>22205877</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/29255254/>29255254</a> Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. Dietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. Nat Microbiol. 2018 Feb;3(2):210-219. doi: 10.1038/s41564-017-0079-1. Epub 2017 Dec 18.	2011 Dec,2018 Feb	degradation	7	3	GH147, GH2, GH53
PUL0642	recombinant protein expression	starch	Roseburia intestinalis L1-82	<a href=https://pubmed.ncbi.nlm.nih.gov/33439065/>33439065</a> Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503.	2021 Jan-Dec	degradation	11	6	CBM61, GH53, CBM86, CBM22, GH10, CBM9, GH13_18, GH13_31, GH32, GH36
PUL0768	RNA-seq, qRT-PCR, gas chromatography, mass spectrometry, thin-layer chromatography	pectin	Segatella copri DSM 18205	<a href=https://pubmed.ncbi.nlm.nih.gov/39636128/>39636128</a> Transcriptional delineation of polysaccharide utilization loci in the human gut commensal Segatella copri DSM18205 and co-culture with exemplar Bacteroides species on dietary plant glycans. Appl Environ Microbiol. 2025 Jan 31;91(1):e0175924. doi: 10.1128/aem.01759-24. Epub 2024 Dec 5.	2025 Jan 31	degradation	7	3	GH2, GH53, PL1, CBM77