| PULID | Characterization Method(s) | Substrate | Organism | Publication | Publish Date | Type | Num Genes | Num CAZymes | CazyFamily |
|---|---|---|---|---|---|---|---|---|---|
| PUL0049 | fosmid library screen | beta-glucan | feces metagenome | <a href=https://pubmed.ncbi.nlm.nih.gov/29601586/>29601586</a> Two new gene clusters involved in the degradation of plant cell wall from the fecal microbiota of Tunisian dromedary. PLoS One. 2018 Mar 30;13(3):e0194621. doi: 10.1371/journal.pone.0194621. eCollection 2018. |
2018 | degradation | 29 | 5 | CE20, GH16_3, GH26, GH43_17 |
| PUL0545 | RNA-seq | arabinoxylan | Bacteroides cellulosilyticus | <a href=https://pubmed.ncbi.nlm.nih.gov/23976882/>23976882</a> Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20. |
2013 | degradation | 10 | 6 | CE1, GH3, GH43_17, GH43_2, CBM6, GH43_7, GH43_7, GH9 |
| PUL0643 | enzyme activity assay, liquid chromatography, high-performance anion-exchange chromatography, qRT-PCR, crystallization | arabinoxylan | Bacteroides intestinalis DSM 17393 | <a href=https://pubmed.ncbi.nlm.nih.gov/33469030/>33469030</a> Degradation of complex arabinoxylans by human colonic Bacteroidetes. Nat Commun. 2021 Jan 19;12(1):459. doi: 10.1038/s41467-020-20737-5. |
2021 Jan 19 | degradation | 12 | 7 | CE1, CE6, CE1, GH3, GH43_17, GH43_2, CBM6, GH43_7, GH43_7 |
| PUL0644 | enzyme activity assay, liquid chromatography, high-performance anion-exchange chromatography, qRT-PCR, crystallization | arabinoxylan | Bacteroides cellulosilyticus DSM 14838 | <a href=https://pubmed.ncbi.nlm.nih.gov/33469030/>33469030</a> Degradation of complex arabinoxylans by human colonic Bacteroidetes. Nat Commun. 2021 Jan 19;12(1):459. doi: 10.1038/s41467-020-20737-5. |
2021 Jan 19 | degradation | 12 | 8 | CE1, GH3, GH43_17, GH43_2, CBM6, GH43_7, GH43_7, GH9 |
| PUL0645 | enzyme activity assay, liquid chromatography, high-performance anion-exchange chromatography, qRT-PCR, crystallization | arabinoxylan | Bacteroides oleiciplenus YIT 12058 | <a href=https://pubmed.ncbi.nlm.nih.gov/33469030/>33469030</a> Degradation of complex arabinoxylans by human colonic Bacteroidetes. Nat Commun. 2021 Jan 19;12(1):459. doi: 10.1038/s41467-020-20737-5. |
2021 Jan 19 | degradation | 10 | 5 | CE1, GH3, GH43_17, GH43_2, CBM6, GH43_7, GH43_7 |
| PUL0663 | thin-layer chromatography, clone and expression, recombinant protein expression | arabinogalactan | Bacteroides plebeius DSM17135 | <a href=https://pubmed.ncbi.nlm.nih.gov/34340552/>34340552</a> Sulfation of Arabinogalactan Proteins Confers Privileged Nutrient Status to Bacteroides plebeius. mBio. 2021 Aug 31;12(4):e0136821. doi: 10.1128/mBio.01368-21. Epub 2021 Aug 3. |
2021 Aug 31 | degradation | 7 | 4 | GH154, GH43_17, GH43_24, PL42 |
| PUL0690 | electrophoretic mobility shift assay, qRT-PCR, gene deletion mutant and growth assay | raffinose family oligosaccharides | Bacteroides thetaiotaomicron VPI-5482 | <a href=https://pubmed.ncbi.nlm.nih.gov/34251866/>34251866</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/37598339/>37598339</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/39330254/>39330254</a> A Novel Family of RNA-Binding Proteins Regulate Polysaccharide Metabolism in Bacteroides thetaiotaomicron. Dynamic genetic adaptation of Bacteroides thetaiotaomicron during murine gut colonization. Determinants of raffinose family oligosaccharide use in Bacteroides species. J Bacteriol. 2021 Oct 12;203(21):e0021721. doi: 10.1128/JB.00217-21. Epub 2021 Jul 12. Cell Rep. 2023 Aug 29;42(8):113009. doi: 10.1016/j.celrep.2023.113009. Epub 2023 Aug 21. J Bacteriol. 2024 Oct 24;206(10):e0023524. doi: 10.1128/jb.00235-24. Epub 2024 Sep 27. |
2021 Oct 12,2023 Aug 29,2024 Oct 24 | degradation | 8 | 4 | GH3, GH43_17, GH92, GH97 |
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