| PULID | Characterization Method(s) | Substrate | Organism | Publication | Publish Date | Type | Num Genes | Num CAZymes | CazyFamily |
|---|---|---|---|---|---|---|---|---|---|
| PUL0008 | enzyme activity assay, qPCR, thin-layer chromatography, substrate binding assay | fructan | Bacteroides thetaiotaomicron | <a href=https://pubmed.ncbi.nlm.nih.gov/28103254/>28103254</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/25841008/>25841008</a> A Highly Active Endo-Levanase BT1760 of a Dominant Mammalian Gut Commensal Bacteroides thetaiotaomicron Cleaves Not Only Various Bacterial Levans, but Also Levan of Timothy Grass. Differential Metabolism of Exopolysaccharides from Probiotic Lactobacilli by the Human Gut Symbiont Bacteroides thetaiotaomicron. PLoS One. 2017 Jan 19;12(1):e0169989. doi: 10.1371/journal.pone.0169989. eCollection 2017. Appl Environ Microbiol. 2015 Jun 15;81(12):3973-83. doi: 10.1128/AEM.00149-15. Epub 2015 Apr 3. |
2017,2015 Jun 15 | degradation | 12 | 3 | GH32 |
| PUL0024 | enzyme activity assay, qPCR, carbohydrate binding assay | fructan | uncultured bacterium | <a href=https://pubmed.ncbi.nlm.nih.gov/31915220/>31915220</a> Harvesting of Prebiotic Fructooligosaccharides by Nonbeneficial Human Gut Bacteria. mSphere. 2020 Jan 8;5(1):e00771-19. doi: 10.1128/mSphere.00771-19. |
2020 Jan 8 | degradation | 12 | 1 | GH32 |
| PUL0031 | RNA-seq | starch | Bifidobacterium longum | <a href=https://pubmed.ncbi.nlm.nih.gov/16523284/>16523284</a> A functional analysis of the Bifidobacterium longum cscA and scrP genes in sucrose utilization. Appl Microbiol Biotechnol. 2006 Oct;72(5):975-81. doi: 10.1007/s00253-006-0358-x. Epub 2006 Mar 8. |
2006 Oct | degradation | 3 | 1 | GH32 |
| PUL0068 | enzyme activity assay, electrophoretic mobility shift assay | raffinose | Escherichia coli | <a href=https://pubmed.ncbi.nlm.nih.gov/8277949/>8277949</a> Role of two operators in regulating the plasmid-borne raf operon of Escherichia coli. Mol Gen Genet. 1994 Jan;242(1):90-9. doi: 10.1007/BF00277352. |
1994 Jan | degradation | 4 | 2 | GH32, GH36 |
| PUL0219 | sugar utilization assay, enzyme activity assay | fructan | Lactobacillus paracasei | <a href=https://pubmed.ncbi.nlm.nih.gov/17644636/>17644636</a> Functional analysis of the fructooligosaccharide utilization operon in Lactobacillus paracasei 1195. Appl Environ Microbiol. 2007 Sep;73(18):5716-24. doi: 10.1128/AEM.00805-07. Epub 2007 Jul 20. |
2007 Sep | degradation | 7 | 1 | GH32 |
| PUL0277 | gene deletion mutant and growth assay, qRT-PCR | fructan | Streptococcus pneumoniae | <a href=https://pubmed.ncbi.nlm.nih.gov/23264576/>23264576</a> The ABC transporter encoded at the pneumococcal fructooligosaccharide utilization locus determines the ability to utilize long- and short-chain fructooligosaccharides. J Bacteriol. 2013 Mar;195(5):1031-41. doi: 10.1128/JB.01560-12. Epub 2012 Dec 21. |
2013 Mar | degradation | 5 | 1 | GH32 |
| PUL0278 | gene deletion mutant and growth assay, qRT-PCR | fructan | Streptococcus pneumoniae | <a href=https://pubmed.ncbi.nlm.nih.gov/23264576/>23264576</a> The ABC transporter encoded at the pneumococcal fructooligosaccharide utilization locus determines the ability to utilize long- and short-chain fructooligosaccharides. J Bacteriol. 2013 Mar;195(5):1031-41. doi: 10.1128/JB.01560-12. Epub 2012 Dec 21. |
2013 Mar | degradation | 6 | 1 | GH32 |
| PUL0279 | gene deletion mutant and growth assay, qRT-PCR | fructan | Streptococcus pneumoniae | <a href=https://pubmed.ncbi.nlm.nih.gov/23264576/>23264576</a> The ABC transporter encoded at the pneumococcal fructooligosaccharide utilization locus determines the ability to utilize long- and short-chain fructooligosaccharides. J Bacteriol. 2013 Mar;195(5):1031-41. doi: 10.1128/JB.01560-12. Epub 2012 Dec 21. |
2013 Mar | degradation | 6 | 1 | GH32 |
| PUL0312 | RT-PCR | starch | Gluconacetobacter diazotrophicus | <a href=https://pubmed.ncbi.nlm.nih.gov/19139238/>19139238</a> Transcriptional regulation and signal-peptide-dependent secretion of exolevanase (LsdB) in the endophyte Gluconacetobacter diazotrophicus. Appl Environ Microbiol. 2009 Mar;75(6):1782-5. doi: 10.1128/AEM.01887-08. Epub 2009 Jan 9. |
2009 Mar | degradation | 2 | 2 | GH32, GH68 |
| PUL0341 | fosmid library screen, enzyme activity assay, thin-layer chromatography | beta-glucan | uncultured bacterium | <a href=https://pubmed.ncbi.nlm.nih.gov/28091525/>28091525</a> A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 43 | 3 | GH32, GH5_2, GH91 |
| PUL0351 | enzyme activity assay | starch | Escherichia coli | <a href=https://pubmed.ncbi.nlm.nih.gov/1435727/>1435727</a> Characterization of a chromosomally encoded, non-PTS metabolic pathway for sucrose utilization in Escherichia coli EC3132. Mol Gen Genet. 1992 Oct;235(1):22-32. doi: 10.1007/BF00286177. |
1992 Oct | degradation | 4 | 1 | GH32 |
| PUL0373 | enzyme activity assay | starch | Dickeya dadantii | <a href=https://pubmed.ncbi.nlm.nih.gov/19734309/>19734309</a> Catabolism of raffinose, sucrose, and melibiose in Erwinia chrysanthemi 3937. J Bacteriol. 2009 Nov;191(22):6960-7. doi: 10.1128/JB.00594-09. Epub 2009 Sep 4. |
2009 Nov | degradation | 5 | 1 | GH32 |
| PUL0394 | microarray | fructan | Roseburia inulinivorans | <a href=https://pubmed.ncbi.nlm.nih.gov/20679207/>20679207</a> Substrate-driven gene expression in Roseburia inulinivorans: importance of inducible enzymes in the utilization of inulin and starch. Proc Natl Acad Sci U S A. 2011 Mar 15;108 Suppl 1(Suppl 1):4672-9. doi: 10.1073/pnas.1000091107. Epub 2010 Aug 2. |
2011 Mar 15 | degradation | 6 | 1 | GH32 |
| PUL0425 | microarray | fructan | Bacteroides ovatus | <a href=https://pubmed.ncbi.nlm.nih.gov/22205877/>22205877</a> Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. |
2011 Dec | degradation | 11 | 4 | GH32, GH91 |
| PUL0568 | clone and expression, enzyme activity assay, Northern Blot | starch | Clostridium beijerinckii | <a href=https://pubmed.ncbi.nlm.nih.gov/10411273/>10411273</a> The genes controlling sucrose utilization in Clostridium beijerinckii NCIMB 8052 constitute an operon. Microbiology (Reading). 1999 Jun;145 ( Pt 6):1461-1472. doi: 10.1099/13500872-145-6-1461. |
1999 Jun | degradation | 4 | 1 | GH32 |
| PUL0569 | clone and expression, enzyme activity assay, Northern Blot | fructan | Bacillus subtilis | <a href=https://pubmed.ncbi.nlm.nih.gov/11739774/>11739774</a> yveB, Encoding endolevanase LevB, is part of the sacB-yveB-yveA levansucrase tricistronic operon in Bacillus subtilis. Microbiology (Reading). 2001 Dec;147(Pt 12):3413-9. doi: 10.1099/00221287-147-12-3413. |
2001 Dec | degradation | 4 | 2 | GH32, GH68_1 |
| PUL0581 | SDS-PAGE, enzyme activity assay | fructan | Microbulbifer sp. JAM-3301 | <a href=https://pubmed.ncbi.nlm.nih.gov/22286980/>22286980</a> Cloning and sequencing of inulinase and beta-fructofuranosidase genes of a deep-sea Microbulbifer species and properties of recombinant enzymes. Appl Environ Microbiol. 2012 Apr;78(7):2493-5. doi: 10.1128/AEM.07442-11. Epub 2012 Jan 27. |
2012 Apr | degradation | 3 | 2 | GH32 |
| PUL0587 | RT-PCR, enzyme activity assay | fructan | Prevotella intermedia | <a href=https://pubmed.ncbi.nlm.nih.gov/23266804/>23266804</a> Identification and functional analysis of the gene cluster for fructan utilization in Prevotella intermedia. Gene. 2013 Feb 25;515(2):291-7. doi: 10.1016/j.gene.2012.12.023. Epub 2012 Dec 22. |
2013 Feb 25 | degradation | 3 | 1 | GH32 |
| PUL0604 | microarray, qRT-PCR, culureing methods | galactomannan | Lactobacillus plantarum WCFS1 | <a href=https://pubmed.ncbi.nlm.nih.gov/31703861/>31703861</a> Transcriptional analysis of galactomannooligosaccharides utilization by Lactobacillus plantarum WCFS1. Food Microbiol. 2020 Apr;86:103336. doi: 10.1016/j.fm.2019.103336. Epub 2019 Sep 14. |
2020 Apr | degradation | 5 | 2 | GH13_31, GH32 |
| PUL0632 | recombinant protein expression | fructan | Roseburia inulinivorans DSM 16841 | <a href=https://pubmed.ncbi.nlm.nih.gov/33439065/>33439065</a> Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 5 | 1 | GH32 |
| PUL0633 | recombinant protein expression | fructan | Roseburia faecis M72 | <a href=https://pubmed.ncbi.nlm.nih.gov/33439065/>33439065</a> Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 5 | 1 | GH32 |
| PUL0634 | recombinant protein expression | fructan | Eubacterium rectale ATCC 33656 | <a href=https://pubmed.ncbi.nlm.nih.gov/33439065/>33439065</a> Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 6 | 1 | GH32 |
| PUL0635 | recombinant protein expression | fructan | Coprococcus eutactus JCM 31265 | <a href=https://pubmed.ncbi.nlm.nih.gov/33439065/>33439065</a> Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 7 | 1 | GH32 |
| PUL0636 | recombinant protein expression | fructan | Coprococcus eutactus JCM 31265 | <a href=https://pubmed.ncbi.nlm.nih.gov/33439065/>33439065</a> Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 9 | 1 | GH32 |
| PUL0637 | recombinant protein expression | fructan | Faecalibacterium prausnitzii A2165 | <a href=https://pubmed.ncbi.nlm.nih.gov/33439065/>33439065</a> Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 5 | 1 | GH32 |
| PUL0638 | recombinant protein expression | fructan | Anaerostipes hadrus DSM 3319 | <a href=https://pubmed.ncbi.nlm.nih.gov/33439065/>33439065</a> Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 5 | 1 | GH32 |
| PUL0639 | recombinant protein expression | fructan | Anaerostipes hadrus DSM 3319 | <a href=https://pubmed.ncbi.nlm.nih.gov/33439065/>33439065</a> Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 4 | 2 | GH32 |
| PUL0640 | recombinant protein expression | fructan | Anaerostipes hadrus DSM 3319 | <a href=https://pubmed.ncbi.nlm.nih.gov/33439065/>33439065</a> Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 10 | 1 | CBM66, GH32, CBM66 |
| PUL0641 | recombinant protein expression | fructan | Anaerostipes caccae L1-92 DSM 14662 | <a href=https://pubmed.ncbi.nlm.nih.gov/33439065/>33439065</a> Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 10 | 1 | GH32 |
| PUL0642 | recombinant protein expression | starch | Roseburia intestinalis L1-82 | <a href=https://pubmed.ncbi.nlm.nih.gov/33439065/>33439065</a> Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 11 | 6 | CBM61, GH53, CBM86, CBM22, GH10, CBM9, GH13_18, GH13_31, GH32, GH36 |
| PUL0678 | RNA-seq, thin-layer chromatography, growth assay | inulin | Lactiplantibacillus plantarum QS7T | <a href=https://pubmed.ncbi.nlm.nih.gov/34980384/>34980384</a> Global genome and comparative transcriptomic analysis reveal the inulin consumption strategy of Lactiplantibacillus plantarum QS7T. Food Res Int. 2022 Jan;151:110846. doi: 10.1016/j.foodres.2021.110846. Epub 2021 Dec 2. |
2022 Jan | degradation | 5 | 2 | GH32, GH36 |
| PUL0679 | RNA-seq, thin-layer chromatography, growth assay | inulin | Lactiplantibacillus plantarum QS7T | <a href=https://pubmed.ncbi.nlm.nih.gov/34980384/>34980384</a> Global genome and comparative transcriptomic analysis reveal the inulin consumption strategy of Lactiplantibacillus plantarum QS7T. Food Res Int. 2022 Jan;151:110846. doi: 10.1016/j.foodres.2021.110846. Epub 2021 Dec 2. |
2022 Jan | degradation | 7 | 1 | GH32 |
| PUL0752 | thin-layer chromatography, HPAEC-PAD, recombinant protein expression, in vitro assimilation | fructo-disaccharide | Blautia parvula NBRC 113351 | <a href=https://pubmed.ncbi.nlm.nih.gov/39500763/>39500763</a> Degradation mechanism of difructose dianhydride III in Blautia species. Appl Microbiol Biotechnol. 2024 Nov 5;108(1):502. doi: 10.1007/s00253-024-13346-5. |
2024 Nov 5 | degradation | 10 | 3 | GH32, GH39, GH91 |
| PUL0753 | in vitro assimilation | fructo-disaccharide | Blautia hansenii DSM 20583 | <a href=https://pubmed.ncbi.nlm.nih.gov/39500763/>39500763</a> Degradation mechanism of difructose dianhydride III in Blautia species. Appl Microbiol Biotechnol. 2024 Nov 5;108(1):502. doi: 10.1007/s00253-024-13346-5. |
2024 Nov 5 | degradation | 11 | 2 | GH32, GH91 |
| PUL0754 | in vitro assimilation | fructo-disaccharide | Blautia hydrogenotrophica DSM 10507 | <a href=https://pubmed.ncbi.nlm.nih.gov/39500763/>39500763</a> Degradation mechanism of difructose dianhydride III in Blautia species. Appl Microbiol Biotechnol. 2024 Nov 5;108(1):502. doi: 10.1007/s00253-024-13346-5. |
2024 Nov 5 | degradation | 9 | 2 | GH32, GH91 |
| PUL0755 | in vitro assimilation | fructo-disaccharide | Blautia wexlerae JCM 35486 | <a href=https://pubmed.ncbi.nlm.nih.gov/39500763/>39500763</a> Degradation mechanism of difructose dianhydride III in Blautia species. Appl Microbiol Biotechnol. 2024 Nov 5;108(1):502. doi: 10.1007/s00253-024-13346-5. |
2024 Nov 5 | degradation | 12 | 3 | GH120, GH32, GH91 |
| PUL0765 | RNA-seq, qRT-PCR, gas chromatography, mass spectrometry, thin-layer chromatography | inulin | Segatella copri DSM 18205 | <a href=https://pubmed.ncbi.nlm.nih.gov/39636128/>39636128</a> Transcriptional delineation of polysaccharide utilization loci in the human gut commensal Segatella copri DSM18205 and co-culture with exemplar Bacteroides species on dietary plant glycans. Appl Environ Microbiol. 2025 Jan 31;91(1):e0175924. doi: 10.1128/aem.01759-24. Epub 2024 Dec 5. |
2025 Jan 31 | degradation | 10 | 1 | GH32 |
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