Browse dbCAN-PUL Entries
| PULID | Characterization Method(s) | Substrate | Organism | Publication | Publish Date | Type | Num Genes | Num CAZymes | CazyFamily |
|---|---|---|---|---|---|---|---|---|---|
| PUL0012 | enzyme activity assay | chitin | Vibrio cholerae | 28683122 The nucleoid occlusion protein SlmA is a direct transcriptional activator of chitobiose utilization in Vibrio cholerae. PLoS Genet. 2017 Jul 6;13(7):e1006877. doi: 10.1371/journal.pgen.1006877. eCollection 2017 Jul. |
2017 Jul | degradation | 11 | 3 | GH20, GH9, GH94 |
| PUL0091 | sequence homology analysis | host glycan | Phocaeicola vulgatus | 31275257 Investigating Host Microbiota Relationships Through Functional Metagenomics. Front Microbiol. 2019 Jun 7;10:1286. doi: 10.3389/fmicb.2019.01286. eCollection 2019. |
2019 | degradation | 8 | 7 | CE3, CE20, CE9, GH2, GH20, GH20, CBM32, GH92 |
| PUL0092 | sequence homology analysis | host glycan | Phocaeicola vulgatus | 31275257 Investigating Host Microbiota Relationships Through Functional Metagenomics. Front Microbiol. 2019 Jun 7;10:1286. doi: 10.3389/fmicb.2019.01286. eCollection 2019. |
2019 | degradation | 10 | 5 | CBM93, GH33, CE3, CE20, GH2, GH20, GH20, CBM32 |
| PUL0093 | fosmid library screen, lectin binding assay | host glycan | uncultured bacterium | 31275257 Investigating Host Microbiota Relationships Through Functional Metagenomics. Front Microbiol. 2019 Jun 7;10:1286. doi: 10.3389/fmicb.2019.01286. eCollection 2019. |
2019 | degradation | 24 | 7 | CE20, CE9, GH2, GH20, GH92 |
| PUL0094 | fosmid library screen, lectin binding assay | host glycan | uncultured bacterium | 31275257 Investigating Host Microbiota Relationships Through Functional Metagenomics. Front Microbiol. 2019 Jun 7;10:1286. doi: 10.3389/fmicb.2019.01286. eCollection 2019. |
2019 | degradation | 25 | 7 | CBM93, GH33, CE3, CE20, GH171, GH2, GH20, GH27 |
| PUL0095 | fosmid library screen, lectin binding assay | host glycan | uncultured bacterium | 31275257 Investigating Host Microbiota Relationships Through Functional Metagenomics. Front Microbiol. 2019 Jun 7;10:1286. doi: 10.3389/fmicb.2019.01286. eCollection 2019. |
2019 | degradation | 17 | 6 | CBM93, GH33, CE3, CE20, GH2, GH20, GH27 |
| PUL0096 | fosmid library screen, lectin binding assay | host glycan | uncultured bacterium | 31275257 Investigating Host Microbiota Relationships Through Functional Metagenomics. Front Microbiol. 2019 Jun 7;10:1286. doi: 10.3389/fmicb.2019.01286. eCollection 2019. |
2019 | degradation | 22 | 12 | CBM93, GH33, CE3, CE3, CE20, CE9, GH2, GH20, GH29, GH92, GH97 |
| PUL0097 | sequence homology analysis | host glycan | Bacteroides massiliensis | 31275257 Investigating Host Microbiota Relationships Through Functional Metagenomics. Front Microbiol. 2019 Jun 7;10:1286. doi: 10.3389/fmicb.2019.01286. eCollection 2019. |
2019 | degradation | 15 | 10 | CBM93, GH33, CE3, CE3, CE20, CE9, GH2, GH20, GH92 |
| PUL0102 | fosmid library screen, lectin binding assay | host glycan | uncultured bacterium | 31275257 Investigating Host Microbiota Relationships Through Functional Metagenomics. Front Microbiol. 2019 Jun 7;10:1286. doi: 10.3389/fmicb.2019.01286. eCollection 2019. |
2019 | degradation | 25 | 6 | CBM67, GH78, CBM93, GH33, CE20, CE3, GH20, GH29 |
| PUL0106 | fosmid library screen, lectin binding assay | host glycan | uncultured bacterium | 31275257 Investigating Host Microbiota Relationships Through Functional Metagenomics. Front Microbiol. 2019 Jun 7;10:1286. doi: 10.3389/fmicb.2019.01286. eCollection 2019. |
2019 | degradation | 22 | 4 | GH2, GH20, CBM32 |
| PUL0112 | fosmid library screen, lectin binding assay | host glycan | uncultured bacterium | 31275257 Investigating Host Microbiota Relationships Through Functional Metagenomics. Front Microbiol. 2019 Jun 7;10:1286. doi: 10.3389/fmicb.2019.01286. eCollection 2019. |
2019 | degradation | 25 | 4 | GH2, GH20, CBM32 |
| PUL0115 | expression of recombinant proteins, RNA-seq, differential gene expression | host glycan | Bacteroides thetaiotaomicron | 31160824 Complex N-glycan breakdown by gut Bacteroides involves an extensive enzymatic apparatus encoded by multiple co-regulated genetic loci. Nat Microbiol. 2019 Sep;4(9):1571-1581. doi: 10.1038/s41564-019-0466-x. Epub 2019 Jun 3. |
2019 Sep | degradation | 7 | 7 | CBM93, GH33, CE3, CE20, GH2, GH20, GH20, CBM32 |
| PUL0116 | expression of recombinant proteins, RNA-seq, differential gene expression | host glycan | Bacteroides thetaiotaomicron | 31160824 Complex N-glycan breakdown by gut Bacteroides involves an extensive enzymatic apparatus encoded by multiple co-regulated genetic loci. Nat Microbiol. 2019 Sep;4(9):1571-1581. doi: 10.1038/s41564-019-0466-x. Epub 2019 Jun 3. |
2019 Sep | degradation | 2 | 1 | GH20 |
| PUL0117 | expression of recombinant proteins, RNA-seq, differential gene expression, enzyme specificity assay, enzyme activity assay | host glycan | Bacteroides thetaiotaomicron | 31160824 Complex N-glycan breakdown by gut Bacteroides involves an extensive enzymatic apparatus encoded by multiple co-regulated genetic loci. Discovery of beta-1,4-D-mannosyl-N-acetyl-D-glucosamine phosphorylase involved in the metabolism of N-glycans. Nat Microbiol. 2019 Sep;4(9):1571-1581. doi: 10.1038/s41564-019-0466-x. Epub 2019 Jun 3. J Biol Chem. 2013 Sep 20;288(38):27366-27374. doi: 10.1074/jbc.M113.469080. Epub 2013 Aug 13. |
2019 Sep,2013 Sep 20 | degradation | 22 | 7 | GH130_2, GH163, GH18, GH20, GH92 |
| PUL0194 | enzyme activity assay, gene deletion mutant and growth assay | host glycan | Streptococcus pneumoniae | 28056108 Molecular Characterization of N-glycan Degradation and Transport in Streptococcus pneumoniae and Its Contribution to Virulence. PLoS Pathog. 2017 Jan 5;13(1):e1006090. doi: 10.1371/journal.ppat.1006090. eCollection 2017 Jan. |
2017 Jan | degradation | 6 | 5 | GH125, GH20, GH29, GH38, GH92 |
| PUL0196 | enzyme activity assay | human milk oligosaccharide | Lactobacillus casei | 26546429 The Extracellular Wall-Bound beta-N-Acetylglucosaminidase from Lactobacillus casei Is Involved in the Metabolism of the Human Milk Oligosaccharide Lacto-N-Triose. Appl Environ Microbiol. 2015 Nov 6;82(2):570-7. doi: 10.1128/AEM.02888-15. Print 2016 Jan 15. |
2016 Jan 15 | degradation | 10 | 3 | CE9, GH20, GH35 |
| PUL0211 | enzyme activity assay, gene deletion mutant and growth assay, thin-layer chromatography | host glycan | Xanthomonas campestris pv. campestris | 25586188 The N-Glycan cluster from Xanthomonas campestris pv. campestris: a toolbox for sequential plant N-glycan processing. The plant pathogen Xanthomonas campestris pv. campestris exploits N-acetylglucosamine during infection. J Biol Chem. 2015 Mar 6;290(10):6022-36. doi: 10.1074/jbc.M114.624593. Epub 2015 Jan 13. mBio. 2014 Sep 9;5(5):e01527-14. doi: 10.1128/mBio.01527-14. |
2015 Mar 6,2014 Sep 9 | degradation | 9 | 8 | GH125, GH18, GH2, GH20, GH29, GH3, GH35, GH92 |
| PUL0244 | gene deletion mutant and growth assay, complementation study, carbohydrate binding assay | host glycan | Tannerella forsythia | 24351045 Structural and functional characterization of NanU, a novel high-affinity sialic acid-inducible binding protein of oral and gut-dwelling Bacteroidetes species. Biochem J. 2014 Mar 15;458(3):499-511. doi: 10.1042/BJ20131415. |
2014 Mar 15 | degradation | 9 | 3 | CBM93, GH33, CE20, GH20 |
| PUL0339 | fosmid library screen, enzyme activity assay, thin-layer chromatography | xylan | uncultured bacterium | 28091525 A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 24 | 4 | GH16_3, GH20, GH3, GH97 |
| PUL0344 | gene deletion mutant and growth assay, protein structure characterization | chitin | Flavobacterium johnsoniae | 27933102 A polysaccharide utilization locus from Flavobacterium johnsoniae enables conversion of recalcitrant chitin. Structural insights of the enzymes from the chitin utilization locus of Flavobacterium johnsoniae. Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis. Biotechnol Biofuels. 2016 Nov 28;9:260. doi: 10.1186/s13068-016-0674-z. eCollection 2016. Sci Rep. 2020 Aug 13;10(1):13775. doi: 10.1038/s41598-020-70749-w. Appl Environ Microbiol. 2009 Nov;75(21):6864-75. doi: 10.1128/AEM.01495-09. Epub 2009 Aug 28. |
2016,2020 Aug 13,2009 Nov | degradation | 11 | 3 | GH18, GH18, GH18, GH20 |
| PUL0348 | enzyme activity assay | host glycan | Bacteroides fragilis | 22449996 Characterization of a gene cluster for sialoglycoconjugate utilization in Bacteroides fragilis. J Med Invest. 2012;59(1-2):79-94. doi: 10.2152/jmi.59.79. |
2012 | degradation | 13 | 9 | CBM93, GH33, CE3, CE20, GH2, GH20, GH20, CBM32, GH92 |
| PUL0352 | microarray | host glycan | Bacteroides thetaiotaomicron | 16968696 Functional genomic and metabolic studies of the adaptations of a prominent adult human gut symbiont, Bacteroides thetaiotaomicron, to the suckling period. J Biol Chem. 2006 Nov 24;281(47):36269-79. doi: 10.1074/jbc.M606509200. Epub 2006 Sep 12. |
2006 Nov 24 | degradation | 6 | 1 | GH20 |
| PUL0368 | microarray, Western Blot | human milk oligosaccharide | Bifidobacterium longum subsp. infantis | 19033196 The genome sequence of Bifidobacterium longum subsp. infantis reveals adaptations for milk utilization within the infant microbiome. Proc Natl Acad Sci U S A. 2008 Dec 2;105(48):18964-9. doi: 10.1073/pnas.0809584105. Epub 2008 Nov 24. |
2008 Dec 2 | degradation | 30 | 5 | GH2, GH20, GH29, GH33, GH95 |
| PUL0415 | microarray | xylan | Bacteroides ovatus | 22205877 Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. |
2011 Dec | degradation | 4 | 2 | GH20, GH20, CBM32 |
| PUL0464 | microarray, qPCR | host glycan | Bacteroides thetaiotaomicron | 18996345 Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007. |
2008 Nov 13 | degradation | 16 | 8 | CBM93, GH33, CE20, GH2, GH20, GH20, CBM32 |
| PUL0467 | microarray, qPCR, expression of recombinant proteins, RNA-seq, differential gene expression | host glycan | Bacteroides thetaiotaomicron | 18996345 Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Complex N-glycan breakdown by gut Bacteroides involves an extensive enzymatic apparatus encoded by multiple co-regulated genetic loci. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007. Nat Microbiol. 2019 Sep;4(9):1571-1581. doi: 10.1038/s41564-019-0466-x. Epub 2019 Jun 3. |
2008 Nov 13,2019 Sep | degradation | 14 | 4 | GH18, GH2, GH20, GH29, CBM32 |
| PUL0555 | gene deletion mutant and growth assay, qRT-PCR, microarray, enzyme activity assay | host glycan | Bacteroides fragilis | 25139987 Efficient utilization of complex N-linked glycans is a selective advantage for Bacteroides fragilis in extraintestinal infections. Proc Natl Acad Sci U S A. 2014 Sep 2;111(35):12901-6. doi: 10.1073/pnas.1407344111. Epub 2014 Aug 19. |
2014 Sep 2 | degradation | 9 | 5 | GH154, GH2, GH20, GH88, PL33_1 |
| PUL0577 | SDS-PAGE, enzyme activity assay | chitin | Photobacterium profundum | 21098515 Elucidation of exo-beta-D-glucosaminidase activity of a family 9 glycoside hydrolase (PBPRA0520) from Photobacterium profundum SS9. Glycobiology. 2011 Apr;21(4):503-11. doi: 10.1093/glycob/cwq191. Epub 2010 Nov 22. |
2011 Apr | degradation | 11 | 3 | GH20, GH9, GH94 |
| PUL0586 | enzyme activity assay | chitin | Serratia marcescens | 23047109 Regulation of chitinase production by the 5'-untranslated region of the ybfM in Serratia marcescens 2170. Biosci Biotechnol Biochem. 2012;76(10):1920-4. doi: 10.1271/bbb.120403. Epub 2012 Oct 7. |
2012 | degradation | 3 | 1 | GH20 |
| PUL0721 | RNA-seq, RT-qPCR, gene deletion mutant and growth assay | human milk oligosaccharide | Phocaeicola dorei strain DSM 17855 | 38167825 CRISPR-Cas-based identification of a sialylated human milk oligosaccharides utilization cluster in the infant gut commensal Bacteroides dorei. Nat Commun. 2024 Jan 2;15(1):105. doi: 10.1038/s41467-023-44437-y. |
2024 Jan 2 | degradation | 13 | 9 | CBM93, GH33, CE3, CE20, CE9, GH2, GH20, GH92 |
| PUL0746 | high performance gel permeation chromatography, gas chromatography, RNA-seq, differential gene expression | pectic polysaccharide | Bacteroides ovatus strain ATCC 8483 | 38890895 The Utilization by Bacteroides spp. of a Purified Polysaccharide from Fuzhuan Brick Tea. Foods. 2024 May 26;13(11):1666. doi: 10.3390/foods13111666. |
2024 May 26 | degradation | 7 | 4 | CBM93, GH33, GH2, GH20, GH20, CBM32 |
| PUL0747 | high performance gel permeation chromatography, gas chromatography, RNA-seq, differential gene expression | pectic polysaccharide | Bacteroides ovatus strain ATCC 8483 | 38890895 The Utilization by Bacteroides spp. of a Purified Polysaccharide from Fuzhuan Brick Tea. Foods. 2024 May 26;13(11):1666. doi: 10.3390/foods13111666. |
2024 May 26 | degradation | 9 | 3 | GH2, GH20, GH29, CBM32 |
| PUL0756 | RNA-seq, qRT-PCR | N-glycan | Barnesiella intestinihominis | 39510934 Molecular mechanisms of complex-type N-glycan breakdown and metabolism by the human intestinal bacterium Barnesiella intestinihominis. J Biosci Bioeng. 2025 Jan;139(1):14-22. doi: 10.1016/j.jbiosc.2024.10.006. Epub 2024 Nov 7. |
2025 Jan | degradation | 11 | 7 | GH130_2, GH20, GH84, CBM32, GH85, GH92 |
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