| PULID | Characterization Method(s) | Substrate | Organism | Publication | Publish Date | Type | Num Genes | Num CAZymes | CazyFamily |
|---|---|---|---|---|---|---|---|---|---|
| PUL0049 | fosmid library screen | beta-glucan | feces metagenome | <a href=https://pubmed.ncbi.nlm.nih.gov/29601586/>29601586</a> Two new gene clusters involved in the degradation of plant cell wall from the fecal microbiota of Tunisian dromedary. PLoS One. 2018 Mar 30;13(3):e0194621. doi: 10.1371/journal.pone.0194621. eCollection 2018. |
2018 | degradation | 29 | 5 | CE20, GH16_3, GH26, GH43_17 |
| PUL0118 | qRT-PCR, affinity gel electrophoresis, isothermal titration calorimetry (ITC) | beta-glucan | Bacteroides uniformis | <a href=https://pubmed.ncbi.nlm.nih.gov/32265336/>32265336</a> Synergy between Cell Surface Glycosidases and Glycan-Binding Proteins Dictates the Utilization of Specific Beta(1,3)-Glucans by Human Gut Bacteroides. mBio. 2020 Apr 7;11(2):e00095-20. doi: 10.1128/mBio.00095-20. |
2020 Apr 7 | degradation | 7 | 3 | GH158, GH16_3, GH3 |
| PUL0187 | qRT-PCR, enzyme activity assay | beta-glucan | Paenibacillus sp. JDR-2 | <a href=https://pubmed.ncbi.nlm.nih.gov/26746717/>26746717</a> A 1,3-1,4-beta-Glucan Utilization Regulon in Paenibacillus sp. Strain JDR-2. Appl Environ Microbiol. 2016 Jan 8;82(6):1789-1798. doi: 10.1128/AEM.03526-15. |
2016 Jan 8 | degradation | 7 | 2 | GH16_21, SLH, CBM54, GH16_3, CBM4, CBM4, CBM6, CBM4, CBM4 |
| PUL0220 | mass spectrometry, target decoy database analysis | beta-glucan | Polaribacter sp. Hel1_33_49 | <a href=https://pubmed.ncbi.nlm.nih.gov/25478683/>25478683</a> Niches of two polysaccharide-degrading Polaribacter isolates from the North Sea during a spring diatom bloom. ISME J. 2015 Jun;9(6):1410-22. doi: 10.1038/ismej.2014.225. Epub 2014 Dec 5. |
2015 Jun | degradation | 11 | 5 | GH149, GH16_3, GH17, GH3, GH30_1 |
| PUL0234 | proteome fractionation, mass spectrometry, target decoy database analysis | beta-glucan | Gramella forsetii | <a href=https://pubmed.ncbi.nlm.nih.gov/24522261/>24522261</a> Functional characterization of polysaccharide utilization loci in the marine Bacteroidetes 'Gramella forsetii' KT0803. ISME J. 2014 Jul;8(7):1492-502. doi: 10.1038/ismej.2014.4. Epub 2014 Feb 13. |
2014 Jul | degradation | 7 | 3 | GH16_3, GH3 |
| PUL0335 | fosmid library screen, enzyme activity assay, thin-layer chromatography | xylan | uncultured bacterium | <a href=https://pubmed.ncbi.nlm.nih.gov/28091525/>28091525</a> A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 31 | 5 | GH13_46, GH158, GH16_3, GH3, GH97 |
| PUL0336 | fosmid library screen, enzyme activity assay, thin-layer chromatography | xylan | uncultured bacterium | <a href=https://pubmed.ncbi.nlm.nih.gov/28091525/>28091525</a> A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 25 | 4 | GH158, GH16_3, GH3, GT2 |
| PUL0337 | fosmid library screen, enzyme activity assay, thin-layer chromatography | xylan | uncultured bacterium | <a href=https://pubmed.ncbi.nlm.nih.gov/28091525/>28091525</a> A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 29 | 4 | GH158, GH16_3, GH3, GT2 |
| PUL0338 | fosmid library screen, enzyme activity assay, thin-layer chromatography | xylan | uncultured bacterium | <a href=https://pubmed.ncbi.nlm.nih.gov/28091525/>28091525</a> A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 34 | 5 | GH158, GH16_3, GH3, GH97, GT2 |
| PUL0339 | fosmid library screen, enzyme activity assay, thin-layer chromatography | xylan | uncultured bacterium | <a href=https://pubmed.ncbi.nlm.nih.gov/28091525/>28091525</a> A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 24 | 4 | GH16_3, GH20, GH3, GH97 |
| PUL0346 | gene deletion mutant and growth assay | xylan | uncultured bacterium | <a href=https://pubmed.ncbi.nlm.nih.gov/24066026/>24066026</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/27573446/>27573446</a> Functional metagenomics reveals novel pathways of prebiotic breakdown by human gut bacteria. Functional characterization of a gene locus from an uncultured gut Bacteroides conferring xylo-oligosaccharides utilization to Escherichia coli. PLoS One. 2013 Sep 16;8(9):e72766. doi: 10.1371/journal.pone.0072766. eCollection 2013. Mol Microbiol. 2016 Nov;102(4):579-592. doi: 10.1111/mmi.13480. Epub 2016 Sep 14. |
2013,2016 Nov | degradation | 13 | 5 | GH10, GH16_3, GH43_1, GH43_12, CBM91, GH43_29 |
| PUL0376 | microarray, qPCR | mucin | Bacteroides thetaiotaomicron | <a href=https://pubmed.ncbi.nlm.nih.gov/18996345/>18996345</a> Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007. |
2008 Nov 13 | degradation | 9 | 2 | GH16_3, GH18 |
| PUL0401 | RNA-seq | beta-glucan | Alteromonas macleodii | <a href=https://pubmed.ncbi.nlm.nih.gov/30116038/>30116038</a> Biphasic cellular adaptations and ecological implications of Alteromonas macleodii degrading a mixture of algal polysaccharides. ISME J. 2019 Jan;13(1):92-103. doi: 10.1038/s41396-018-0252-4. Epub 2018 Aug 16. |
2019 Jan | degradation | 9 | 3 | GH1, GH16_3, GH3 |
| PUL0406 | high-performance anion-exchange chromatography | beta-glucan | Coprothermobacter proteolyticus | <a href=https://pubmed.ncbi.nlm.nih.gov/30315317/>30315317</a> From proteins to polysaccharides: lifestyle and genetic evolution of Coprothermobacter proteolyticus. ISME J. 2019 Mar;13(3):603-617. doi: 10.1038/s41396-018-0290-y. Epub 2018 Oct 12. |
2019 Mar | degradation | 21 | 3 | GH16_3, GH18, GH3 |
| PUL0435 | mass spectrometry, high-performance anion-exchange chromatography | beta-glucan | Chitinophaga pinensis | <a href=https://pubmed.ncbi.nlm.nih.gov/28069559/>28069559</a> Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6. |
2017 Mar 6 | degradation | 13 | 4 | GH16_3, GH30_1, GH30_3, GH43_34 |
| PUL0442 | mass spectrometry, high-performance anion-exchange chromatography, SDS-PAGE, recombinant protein expression, enzyme activity assay, substrate binding assay | glucomannan/chitin | Chitinophaga pinensis DSM 2588 | <a href=https://pubmed.ncbi.nlm.nih.gov/28069559/>28069559</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/37493618/>37493618</a> Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. A polysaccharide utilization locus from Chitinophaga pinensis simultaneously targets chitin and beta-glucans found in fungal cell walls. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6. mSphere. 2023 Aug 24;8(4):e0024423. doi: 10.1128/msphere.00244-23. Epub 2023 Jul 26. |
2017 Mar 6,2023 Aug 24 | degradation | 9 | 3 | GH16_3, CBM6, GH18, CBM6, GH18, GH18, CBM5 |
| PUL0443 | mass spectrometry, high-performance anion-exchange chromatography | glucomannan | Chitinophaga pinensis | <a href=https://pubmed.ncbi.nlm.nih.gov/28069559/>28069559</a> Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6. |
2017 Mar 6 | degradation | 9 | 3 | CBM6, CBM6, GH16_3, CBM32, GH16_3, CBM92 |
| PUL0458 | RNA-seq, analysis of reaction products, enzyme activity assay | carrageenan | Colwellia echini | <a href=https://pubmed.ncbi.nlm.nih.gov/31915221/>31915221</a> A Multifunctional Polysaccharide Utilization Gene Cluster in Colwellia echini Encodes Enzymes for the Complete Degradation of kappa-Carrageenan, iota-Carrageenan, and Hybrid beta/kappa-Carrageenan. mSphere. 2020 Jan 8;5(1):e00792-19. doi: 10.1128/mSphere.00792-19. |
2020 Jan 8 | degradation | 46 | 9 | GH16_13, GH16_13, CBM16, CBM16, GH16_17, GH16_3, GH167, GH82 |
| PUL0477 | growth assay | beta-glucan | Flavobacterium johnsoniae | <a href=https://pubmed.ncbi.nlm.nih.gov/19717629/>19717629</a> Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis. Appl Environ Microbiol. 2009 Nov;75(21):6864-75. doi: 10.1128/AEM.01495-09. Epub 2009 Aug 28. |
2009 Nov | degradation | 6 | 2 | GH16_3 |
| PUL0528 | microarray, qPCR | beta-glucan | Bacteroides ovatus | <a href=https://pubmed.ncbi.nlm.nih.gov/22205877/>22205877</a> Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. |
2011 Dec | degradation | 7 | 3 | GH16_3, GH3 |
| PUL0535 | RNA-seq | arabinogalactan | Bacteroides cellulosilyticus | <a href=https://pubmed.ncbi.nlm.nih.gov/23976882/>23976882</a> Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20. |
2013 | degradation | 9 | 3 | CBM32, GH16_3, GH43_24, GH16_3 |
| PUL0551 | RNA-seq | beta-glucan | Bacteroides cellulosilyticus | <a href=https://pubmed.ncbi.nlm.nih.gov/23976882/>23976882</a> Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20. |
2013 | degradation | 6 | 2 | GH16_3, GH3 |
| PUL0563 | microarray, qPCR | arabinogalactan | Bacteroides thetaiotaomicron | <a href=https://pubmed.ncbi.nlm.nih.gov/18996345/>18996345</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/22205877/>22205877</a> Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. |
2008 Nov 13,2011 Dec | degradation | 15 | 7 | GH105, GH127, GH154, GH16_3, GH43_24, GH43_24, GH43_34, PL42 |
| PUL0651 | enzyme activity assay, NMR | agarose | Gilvimarinus chinensis DSM 19667 | <a href=https://pubmed.ncbi.nlm.nih.gov/33691998/>33691998</a> Agarase cocktail from agar polysaccharide utilization loci converts homogenized Gelidium amansii into neoagarooligosaccharides. Food Chem. 2021 Aug 1;352:128685. doi: 10.1016/j.foodchem.2020.128685. Epub 2020 Nov 19. |
2021 Aug 1 | degradation | 63 | 15 | CBM6, CBM6, CBM6, GH86, GH86, CE1, GH117, GH127, GH16_16, CBM13, GH16_16, CBM6, CBM6, GH16_3, GH167, GH2, GH50, GH86 |
| PUL0674 | microarray, enzyme activity assay, high-performance anion-exchange chromatography, mass spectrometry, RNA-seq, affinity gel electrophoresis, carbohydrate binding assay, microscale thermophoresis | beta-glucan | Bacteroides ovatus ATCC 8483 | <a href=https://pubmed.ncbi.nlm.nih.gov/34817219/>34817219</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/29020628/>29020628</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/22205877/>22205877</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/32801182/>32801182</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/31062073/>31062073</a> Mapping Molecular Recognition of beta1,3-1,4-Glucans by a Surface Glycan-Binding Protein from the Human Gut Symbiont Bacteroides ovatus. Molecular Mechanism by which Prominent Human Gut Bacteroidetes Utilize Mixed-Linkage Beta-Glucans, Major Health-Promoting Cereal Polysaccharides. Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. Sharing a beta-Glucan Meal: Transcriptomic Eavesdropping on a Bacteroides ovatus-Subdoligranulum variabile-Hungatella hathewayi Consortium. Surface glycan-binding proteins are essential for cereal beta-glucan utilization by the human gut symbiont Bacteroides ovatus. Microbiol Spectr. 2021 Dec 22;9(3):e0182621. doi: 10.1128/Spectrum.01826-21. Epub 2021 Nov 24. Cell Rep. 2017 Oct 10;21(2):417-430. doi: 10.1016/j.celrep.2017.09.049. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. Appl Environ Microbiol. 2020 Oct 1;86(20):e01651-20. doi: 10.1128/AEM.01651-20. Print 2020 Oct 1. Cell Mol Life Sci. 2019 Nov;76(21):4319-4340. doi: 10.1007/s00018-019-03115-3. Epub 2019 May 6. |
2021 Dec 22,2017 Oct 10,2011 Dec,2020 Oct 1,2019 Nov | degradation | 8 | 3 | GH16_3, GH3 |
| PUL0749 | affinity gel electrophoresis | beta-glucan | uncultured bacterium | <a href=https://pubmed.ncbi.nlm.nih.gov/39012103/>39012103</a> Biochemical characterization of a SusD-like protein involved in beta-1,3-glucan utilization by an uncultured cow rumen Bacteroides. mSphere. 2024 Aug 28;9(8):e0027824. doi: 10.1128/msphere.00278-24. Epub 2024 Jul 16. |
2024 Aug 28 | degradation | 5 | 2 | GH16_3, GH3 |
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