Browse dbCAN-PUL Entries
| PULID | Characterization Method(s) | Substrate | Organism | Publication | Publish Date | Type | Num Genes | Num CAZymes | CazyFamily |
|---|---|---|---|---|---|---|---|---|---|
| PUL0001 | RNA-seq, substrate binding assay, enzyme activity assay, mass spectrometry | beta-mannan | Roseburia intestinalis | 30796211 The human gut Firmicute Roseburia intestinalis is a primary degrader of dietary beta-mannans. Nat Commun. 2019 Feb 22;10(1):905. doi: 10.1038/s41467-019-08812-y. |
2019 Feb 22 | degradation | 15 | 9 | CE17, CBM35inCE17, CE2, GH1, GH113, GH130_1, GH130_2, GH36 |
| PUL0030 | isothermal calorimetric titration, gene deletion mutant and growth assay, enzyme activity assay | galactomannan | Bacillus sp. N16-5 | 26978267 A Novel Manno-Oligosaccharide Binding Protein Identified in Alkaliphilic Bacillus sp. N16-5 Is Involved in Mannan Utilization. Galactomannan Degrading Enzymes from the Mannan Utilization Gene Cluster of Alkaliphilic Bacillus sp. N16-5 and Their Synergy on Galactomannan Degradation. Transcriptional regulation of the mannan utilization genes in the alkaliphilic Bacillus sp. N16-5. PLoS One. 2016 Mar 15;11(3):e0150059. doi: 10.1371/journal.pone.0150059. eCollection 2016. J Agric Food Chem. 2018 Oct 24;66(42):11055-11063. doi: 10.1021/acs.jafc.8b03878. Epub 2018 Oct 15. FEMS Microbiol Lett. 2018 Feb 1;365(4). doi: 10.1093/femsle/fnx280. |
2016,2018 Oct 24,2018 Feb 1 | degradation | 12 | 6 | CE7, GH130_1, GH130_2, GH27 |
| PUL0050 | fosmid library screen | cellulose | feces metagenome | 29601586 Two new gene clusters involved in the degradation of plant cell wall from the fecal microbiota of Tunisian dromedary. PLoS One. 2018 Mar 30;13(3):e0194621. doi: 10.1371/journal.pone.0194621. eCollection 2018. |
2018 | degradation | 20 | 6 | GH130_1, GH26, GH3, GH5_4, GH94 |
| PUL0129 | enzyme activity assay | beta-mannan | gut metagenome | 30356154 Interspecies cross-feeding orchestrates carbon degradation in the rumen ecosystem. Nat Microbiol. 2018 Nov;3(11):1274-1284. doi: 10.1038/s41564-018-0225-4. Epub 2018 Oct 24. |
2018 Nov | degradation | 12 | 6 | CE7, GH130_1, GH26, GH5_4 |
| PUL0164 | mass spectrometry, sequence homology analysis, differential gene expression | beta-mannan | Leeuwenhoekiella sp. MAR_2009_132 | 30246424 Alpha- and beta-mannan utilization by marine Bacteroidetes. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16. |
2018 Nov | degradation | 19 | 12 | CBM8, CE2, CE20, GH130_1, GH26, GH27, GH3, GH5_2, GH5_7, GH9 |
| PUL0165 | mass spectrometry, sequence homology analysis, differential gene expression | beta-mannan | Salegentibacter sp. Hel_I_6 | 30246424 Alpha- and beta-mannan utilization by marine Bacteroidetes. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16. |
2018 Nov | degradation | 16 | 8 | CE20, GH130_1, GH26, GH27, GH30, GH5_2, GH9 |
| PUL0167 | mass spectrometry, sequence homology analysis | beta-mannan | Bacteroides ovatus | 30246424 Alpha- and beta-mannan utilization by marine Bacteroidetes. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16. |
2018 Nov | degradation | 12 | 4 | GH130_1, GH26, GH36 |
| PUL0175 | enzyme activity assay | galactomannan | Cellvibrio mixtus | 16842369 Galactomannan hydrolysis and mannose metabolism in Cellvibrio mixtus. FEMS Microbiol Lett. 2006 Aug;261(1):123-32. doi: 10.1111/j.1574-6968.2006.00342.x. |
2006 Aug | degradation | 4 | 3 | GH130_1, GH27, GH5_7 |
| PUL0178 | enzyme activity assay, enzyme specificity assay, substrate specificity assay | galactomannan | Bacteroides ovatus | 27288925 A beta-mannan utilization locus in Bacteroides ovatus involves a GH36 alpha-galactosidase active on galactomannans. Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. FEBS Lett. 2016 Jul;590(14):2106-18. doi: 10.1002/1873-3468.12250. Epub 2016 Jun 28. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. |
2016 Jul,2011 Dec | degradation | 14 | 4 | GH130_1, GH26, GH36 |
| PUL0179 | enzyme activity assay, enzyme specificity assay, substrate specificity assay | galactomannan | Bacteroides ovatus | 27288925 A beta-mannan utilization locus in Bacteroides ovatus involves a GH36 alpha-galactosidase active on galactomannans. FEBS Lett. 2016 Jul;590(14):2106-18. doi: 10.1002/1873-3468.12250. Epub 2016 Jun 28. |
2016 Jul | degradation | 15 | 4 | CE7, GH130_1, GH26 |
| PUL0239 | fosmid library screen, sequence homology analysis | cellulose | Prevotella sp. Sc00026 | 24448980 Analysis of the bovine rumen microbiome reveals a diversity of Sus-like polysaccharide utilization loci from the bacterial phylum Bacteroidetes. J Ind Microbiol Biotechnol. 2014 Mar;41(3):601-6. doi: 10.1007/s10295-013-1395-y. Epub 2014 Jan 22. |
2014 Mar | degradation | 16 | 9 | CE20, CE7, GH130_1, GH26, GH26, GH5_4, GH3, GH36, GH5_7 |
| PUL0408 | enzyme activity assay, thin-layer chromatography | beta-mannan | Bacteroides fragilis | 24217874 The mannobiose-forming exo-mannanase involved in a new mannan catabolic pathway in Bacteroides fragilis. Arch Microbiol. 2014 Jan;196(1):17-23. doi: 10.1007/s00203-013-0938-y. Epub 2013 Nov 12. |
2014 Jan | degradation | 4 | 2 | GH130_1, GH26 |
| PUL0419 | microarray | pectin | Bacteroides ovatus | 22205877 Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. |
2011 Dec | degradation | 11 | 7 | CBM67, GH78, GH130_1, GH140, GH2, GH5_2, GH5_5 |
| PUL0488 | growth assay | beta-mannan | Flavobacterium johnsoniae | 19717629 Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis. Appl Environ Microbiol. 2009 Nov;75(21):6864-75. doi: 10.1128/AEM.01495-09. Epub 2009 Aug 28. |
2009 Nov | degradation | 14 | 7 | GH130_1, GH26, GH27, GH5_2, GH5_7, GH97 |
| PUL0538 | RNA-seq | galactomannan | Bacteroides cellulosilyticus | 23976882 Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20. |
2013 | degradation | 15 | 7 | CE7, GH130_1, GH26, GH26, GH26, GH3, GH5_2, GH5_7 |
| PUL0565 | microarray, qPCR | galactomannan | Bacteroides ovatus | 22205877 Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. Galactomannan Catabolism Conferred by a Polysaccharide Utilization Locus of Bacteroides ovatus: ENZYME SYNERGY AND CRYSTAL STRUCTURE OF A beta-MANNANASE. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. J Biol Chem. 2017 Jan 6;292(1):229-243. doi: 10.1074/jbc.M116.746438. Epub 2016 Nov 21. |
2011 Dec,2017 Jan 6 | degradation | 10 | 4 | GH130_1, GH26, GH36 |
| PUL0600 | liquid chromatography and mass spectrometry, differential gene expression | galactomannan | Clostridium cellulovorans | 26020016 Elucidation of the recognition mechanisms for hemicellulose and pectin in Clostridium cellulovorans using intracellular quantitative proteome analysis. AMB Express. 2015 May 23;5:29. doi: 10.1186/s13568-015-0115-6. eCollection 2015. |
2015 | degradation | 12 | 3 | GH130_1, GH130_2, GH2 |
| PUL0658 | qPCR, growth assay | beta-mannooligosaccharide | Faecalibacterium prausnitzii SL3/3 | 34061597 Human Gut Faecalibacterium prausnitzii Deploys a Highly Efficient Conserved System To Cross-Feed on beta-Mannan-Derived Oligosaccharides. mBio. 2021 Jun 29;12(3):e0362820. doi: 10.1128/mBio.03628-20. Epub 2021 Jun 1. |
2021 Jun 29 | degradation | 14 | 6 | CE17, CBM35inCE17, CBM35inCE17, CE2, GH113, GH130_1, GH130_2, GH36 |
| PUL0662 | thin-layer chromatography, liquid chromatography and mass spectrometry, qPCR, clone and expression | beta-mannan | Phocaeicola dorei DSM 17855 | 34339781 BdPUL12 depolymerizes beta-mannan-like glycans into mannooligosaccharides and mannose, which serve as carbon sources for Bacteroides dorei and gut probiotics. Int J Biol Macromol. 2021 Sep 30;187:664-674. doi: 10.1016/j.ijbiomac.2021.07.172. Epub 2021 Jul 31. |
2021 Sep 30 | degradation | 8 | 4 | CE7, GH130_1, GH26, GH5_7 |
| PUL0698 | clone and expression, high-performance anion-exchange chromatography, crystallization | beta-mannan | Muricauda sp. MAR_2010_75 | 36411326 Marine bacteroidetes use a conserved enzymatic cascade to digest diatom beta-mannan. ISME J. 2023 Feb;17(2):276-285. doi: 10.1038/s41396-022-01342-4. Epub 2022 Nov 21. |
2023 Feb | degradation | 22 | 8 | CE2, GH130_1, GH26, GH27, GH5_26, GH9 |
| PUL0699 | RT-qPCR, high-performance anion-exchange chromatography | beta-mannan | Roseburia hominis A2-183 | 36557749 Cross-Feeding and Enzymatic Catabolism for Mannan-Oligosaccharide Utilization by the Butyrate-Producing Gut Bacterium Roseburia hominis A2-183. Microorganisms. 2022 Dec 16;10(12):2496. doi: 10.3390/microorganisms10122496. |
2022 Dec 16 | degradation | 14 | 7 | CE17, CBM35inCE17, CE2, GH1, GH130_1, GH130_2, GH3, GH36 |
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