| PULID | Characterization Method(s) | Substrate | Organism | Publication | Publish Date | Type | Num Genes | Num CAZymes | CazyFamily |
|---|---|---|---|---|---|---|---|---|---|
| PUL0148 | sequence homology analysis | carrageenan | Zobellia galactanivorans | <a href=https://pubmed.ncbi.nlm.nih.gov/30524390/>30524390</a> Evolutionary Evidence of Algal Polysaccharide Degradation Acquisition by Pseudoalteromonas carrageenovora 9(T) to Adapt to Macroalgal Niches. Front Microbiol. 2018 Nov 22;9:2740. doi: 10.3389/fmicb.2018.02740. eCollection 2018. |
2018 | degradation | 15 | 4 | GH127, GH129 |
| PUL0309 | enzyme activity assay, substrate binding assay, isothermal titration calorimetry (ITC) | arabinan | Caldanaerobius polysaccharolyticus | <a href=https://pubmed.ncbi.nlm.nih.gov/28710263/>28710263</a> Enzymatic Mechanism for Arabinan Degradation and Transport in the Thermophilic Bacterium Caldanaerobius polysaccharolyticus. Appl Environ Microbiol. 2017 Aug 31;83(18):e00794-17. doi: 10.1128/AEM.00794-17. Print 2017 Sep 15. |
2017 Sep 15 | degradation | 12 | 6 | GH127, GH146, GH27, GH43_4, GH51_1 |
| PUL0328 | microarray, gas chromatography, mass spectrometry | xylan | Gramella flava | <a href=https://pubmed.ncbi.nlm.nih.gov/28261179/>28261179</a> Characterization of Potential Polysaccharide Utilization Systems in the Marine Bacteroidetes Gramella Flava JLT2011 Using a Multi-Omics Approach. Front Microbiol. 2017 Feb 14;8:220. doi: 10.3389/fmicb.2017.00220. eCollection 2017. |
2017 | degradation | 10 | 5 | GH127, GH2, GH43, GH43_26, GH5_13 |
| PUL0332 | fosmid library screen, enzyme activity assay, thin-layer chromatography | beta-glucan | uncultured bacterium | <a href=https://pubmed.ncbi.nlm.nih.gov/28091525/>28091525</a> A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 19 | 8 | CE7, GH127, GH2, GH5_2, GH5_7, GH94, GH97 |
| PUL0395 | isothermal calorimetric titration, electrophoretic mobility shift assay, Northern Blot | arabinan | Geobacillus stearothermophilus | <a href=https://pubmed.ncbi.nlm.nih.gov/21460081/>21460081</a> The L-Arabinan utilization system of Geobacillus stearothermophilus. J Bacteriol. 2011 Jun;193(11):2838-50. doi: 10.1128/JB.00222-11. Epub 2011 Apr 1. |
2011 Jun | degradation | 25 | 5 | GH127, GH43_4, GH43_5, GH51_1 |
| PUL0482 | growth assay | pectin | Flavobacterium johnsoniae | <a href=https://pubmed.ncbi.nlm.nih.gov/19717629/>19717629</a> Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis. Appl Environ Microbiol. 2009 Nov;75(21):6864-75. doi: 10.1128/AEM.01495-09. Epub 2009 Aug 28. |
2009 Nov | degradation | 27 | 21 | CBM67, GH78, CE19, CE20, CE8, GH106, GH127, GH137, GH139, GH140, GH142, GH143, GH2, GH28, GH43_18, GH78, GH95, PL1_2, PL29 |
| PUL0558 | gene deletion mutant and growth assay, growth assay, enzyme activity assay | pectin | Bacteroides thetaiotaomicron | <a href=https://pubmed.ncbi.nlm.nih.gov/28329766/>28329766</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/22205877/>22205877</a> Complex pectin metabolism by gut bacteria reveals novel catalytic functions. Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. Nature. 2017 Apr 6;544(7648):65-70. doi: 10.1038/nature21725. Epub 2017 Mar 22. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. |
2017 Apr 6,2011 Dec | degradation | 50 | 21 | CBM67, GH78, CBM67, GH78, GH33, CE19, CE20, GH105, GH106, GH127, GH137, GH2, CBM57, CBM97, GH138, GH139, GH140, GH141, GH143, GH142, GH2, GH28, GH43_18, GH78, GH95, PL1_2 |
| PUL0563 | microarray, qPCR | arabinogalactan | Bacteroides thetaiotaomicron | <a href=https://pubmed.ncbi.nlm.nih.gov/18996345/>18996345</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/22205877/>22205877</a> Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. |
2008 Nov 13,2011 Dec | degradation | 15 | 7 | GH105, GH127, GH154, GH16_3, GH43_24, GH43_24, GH43_34, PL42 |
| PUL0626 | high-performance anion-exchange chromatography | arabinan | Lactobacillus crispatus DSM29598 | <a href=https://pubmed.ncbi.nlm.nih.gov/33119797/>33119797</a> Characterization of two extracellular arabinanases in Lactobacillus crispatus. Appl Microbiol Biotechnol. 2020 Dec;104(23):10091-10103. doi: 10.1007/s00253-020-10979-0. Epub 2020 Oct 29. |
2020 Dec | degradation | 24 | 8 | GH127, GH2, GH27, GH43_26, GH43_4, GH51_1 |
| PUL0651 | enzyme activity assay, NMR | agarose | Gilvimarinus chinensis DSM 19667 | <a href=https://pubmed.ncbi.nlm.nih.gov/33691998/>33691998</a> Agarase cocktail from agar polysaccharide utilization loci converts homogenized Gelidium amansii into neoagarooligosaccharides. Food Chem. 2021 Aug 1;352:128685. doi: 10.1016/j.foodchem.2020.128685. Epub 2020 Nov 19. |
2021 Aug 1 | degradation | 63 | 15 | CBM6, CBM6, CBM6, GH86, GH86, CE1, GH117, GH127, GH16_16, CBM13, GH16_16, CBM6, CBM6, GH16_3, GH167, GH2, GH50, GH86 |
| PUL0716 | growth assay, RT-PCR | arabinan | Mesoflavibacter profundi MTRN7 | <a href=https://pubmed.ncbi.nlm.nih.gov/37550707/>37550707</a> Deep-sea Bacteroidetes from the Mariana Trench specialize in hemicellulose and pectin degradation typically associated with terrestrial systems. Microbiome. 2023 Aug 7;11(1):175. doi: 10.1186/s40168-023-01618-7. |
2023 Aug 7 | degradation | 16 | 8 | GH127, GH43_26, GH43_29, GH43_4, GH43_5, GH51_1, GH51_2, GH97 |
| PUL0720 | HPAEC-PAD, enzyme kinetic analysis, thin-layer chromatography, recombinant protein expression, gene mutant | arabinan | Bifidobacterium longum JCM 1217 | <a href=https://pubmed.ncbi.nlm.nih.gov/24385433/>24385433</a> Characterization of a novel beta-L-arabinofuranosidase in Bifidobacterium longum: functional elucidation of a DUF1680 protein family member. J Biol Chem. 2014 Feb 21;289(8):5240-9. doi: 10.1074/jbc.M113.528711. Epub 2014 Jan 2. |
2014 Feb 21 | degradation | 7 | 3 | GH121, GH127, GH43_29 |
| PUL0730 | enzyme activity assay, recombinant protein expression, NMR, HPLC, clone and expression | carrageenan | Cellulophaga algicola DSM 14237 | <a href=https://pubmed.ncbi.nlm.nih.gov/38442258/>38442258</a> Biocatalytic Conversion of Carrageenans for the Production of 3,6-Anhydro-D-galactose. J Agric Food Chem. 2024 Mar 20;72(11):5816-5827. doi: 10.1021/acs.jafc.3c08613. Epub 2024 Mar 5. |
2024 Mar 20 | degradation | 17 | 3 | GH127, GH16_13 |
| PUL0731 | enzyme activity assay, recombinant protein expression, NMR, HPLC, clone and expression | carrageenan | Saccharicrinis fermentans DSM 9555 | <a href=https://pubmed.ncbi.nlm.nih.gov/38442258/>38442258</a> Biocatalytic Conversion of Carrageenans for the Production of 3,6-Anhydro-D-galactose. J Agric Food Chem. 2024 Mar 20;72(11):5816-5827. doi: 10.1021/acs.jafc.3c08613. Epub 2024 Mar 5. |
2024 Mar 20 | degradation | 20 | 5 | GH110, GH127, GH167, GH2 |
| PUL0732 | enzyme activity assay, recombinant protein expression, NMR, HPLC, clone and expression | carrageenan | Cellulophaga baltica 18 | <a href=https://pubmed.ncbi.nlm.nih.gov/38442258/>38442258</a> Biocatalytic Conversion of Carrageenans for the Production of 3,6-Anhydro-D-galactose. J Agric Food Chem. 2024 Mar 20;72(11):5816-5827. doi: 10.1021/acs.jafc.3c08613. Epub 2024 Mar 5. |
2024 Mar 20 | degradation | 19 | 3 | GH127, GH16_13 |
| PUL0733 | enzyme activity assay, recombinant protein expression, NMR, HPLC, clone and expression | carrageenan | Echinicola pacifica DSM 19836 | <a href=https://pubmed.ncbi.nlm.nih.gov/38442258/>38442258</a> Biocatalytic Conversion of Carrageenans for the Production of 3,6-Anhydro-D-galactose. J Agric Food Chem. 2024 Mar 20;72(11):5816-5827. doi: 10.1021/acs.jafc.3c08613. Epub 2024 Mar 5. |
2024 Mar 20 | degradation | 16 | 2 | GH127, GH129 |
| PUL0734 | enzyme activity assay, recombinant protein expression, NMR, HPLC, clone and expression | carrageenan | Cellulophaga lytica DSM 7489 | <a href=https://pubmed.ncbi.nlm.nih.gov/38442258/>38442258</a> Biocatalytic Conversion of Carrageenans for the Production of 3,6-Anhydro-D-galactose. J Agric Food Chem. 2024 Mar 20;72(11):5816-5827. doi: 10.1021/acs.jafc.3c08613. Epub 2024 Mar 5. |
2024 Mar 20 | degradation | 20 | 3 | GH127, GH129, GH2 |
| PUL0735 | enzyme activity assay, clone and expression, reducing-sugar assay, thin-layer chromatography, SDS-PAGE | arabinan | Bifidobacterium longum subsp. suis DSM 20211 | <a href=https://pubmed.ncbi.nlm.nih.gov/38542148/>38542148</a> Functional Characterization of Endo- and Exo-Hydrolase Genes in Arabinan Degradation Gene Cluster of Bifidobacterium longum subsp. suis. Int J Mol Sci. 2024 Mar 9;25(6):3175. doi: 10.3390/ijms25063175. |
2024 Mar 9 | degradation | 17 | 7 | GH127, GH27, GH43_26, GH43_27, GH43_4, GH51_2 |
| PUL0790 | RNA-seq, reducing-sugar assay, growth assay | pectic polysaccharide | Bacteroides ovatus strain ATCC 8483 | <a href=https://pubmed.ncbi.nlm.nih.gov/39892338/>39892338</a> In vitro fermentation of a purified fraction of polysaccharides from the root of Brassica rapa L. by human gut microbiota and its interaction with Bacteroides ovatus. Food Chem. 2025 May 1;473:143109. doi: 10.1016/j.foodchem.2025.143109. Epub 2025 Jan 27. |
2025 May 1 | degradation | 3 | 3 | GH127, GH141, GH78 |
| PUL0793 | enzyme activity assay, quantification of reaction product reducing ends, RNA-seq, differential gene expression, NMR, MALDI-TOF/MS, gas chromatography, mass spectrometry, bicinchoninic acid (BCA) assay, recombinant protein expression | arabinan | Bacteroides intestinalis DSM 17393 | <a href=https://pubmed.ncbi.nlm.nih.gov/39443715/>39443715</a> In vivo manipulation of human gut Bacteroides fitness by abiotic oligosaccharides. Nat Chem Biol. 2025 Apr;21(4):544-554. doi: 10.1038/s41589-024-01763-6. Epub 2024 Oct 23. |
2025 Apr | degradation | 14 | 6 | CE1, GH127, GH146, GH43_34, CBM32, GH97 |
| PUL0796 | recombinant protein expression, RT-PCR, enzyme activity assay | carrageenan | Paraglaciecola hydrolytica S66 | <a href=https://pubmed.ncbi.nlm.nih.gov/29774012/>29774012</a> A Novel Enzyme Portfolio for Red Algal Polysaccharide Degradation in the Marine Bacterium Paraglaciecola hydrolytica S66(T) Encoded in a Sizeable Polysaccharide Utilization Locus. Front Microbiol. 2018 May 3;9:839. doi: 10.3389/fmicb.2018.00839. eCollection 2018. |
2018 | degradation | 35 | 8 | GH127, GH16_13, GH16_17, GH167, GH82 |
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