| PULID | Characterization Method(s) | Substrate | Organism | Publication | Publish Date | Type | Num Genes | Num CAZymes | CazyFamily |
|---|---|---|---|---|---|---|---|---|---|
| PUL0193 | RNA-seq, RT-PCR, qPCR | pectin | Bacteroides xylanisolvens | 26920945 Unraveling the pectinolytic function of Bacteroides xylanisolvens using a RNA-seq approach and mutagenesis. BMC Genomics. 2016 Feb 27;17:147. doi: 10.1186/s12864-016-2472-1. |
2016 Feb 27 | degradation | 30 | 8 | CE20, GH105, GH117, GH117, GH2, GH28, PL11 |
| PUL0225 | RT-PCR | agarose | Bacteroides plebeius | 23150581 Bacteria of the human gut microbiome catabolize red seaweed glycans with carbohydrate-active enzyme updates from extrinsic microbes. Proc Natl Acad Sci U S A. 2012 Nov 27;109(48):19786-91. doi: 10.1073/pnas.1211002109. Epub 2012 Nov 12. |
2012 Nov 27 | degradation | 36 | 12 | GH105, GH154, GH117, GH117, GH16_12, GH16_14, GH16_16, GH2, GH29, GH50, GH86 |
| PUL0316 | microarray | agarose | Zobellia galactanivorans | 28983288 Gene Expression Analysis of Zobellia galactanivorans during the Degradation of Algal Polysaccharides Reveals both Substrate-Specific and Shared Transcriptome-Wide Responses. Front Microbiol. 2017 Sep 21;8:1808. doi: 10.3389/fmicb.2017.01808. eCollection 2017. |
2017 | degradation | 15 | 2 | GH117, GH117, GH2 |
| PUL0459 | RNA-seq, analysis of reaction products, enzyme activity assay, thin-layer chromatography, liquid chromatography, mass spectrometry | agarose | Colwellia echini A3 | 31915221 A Multifunctional Polysaccharide Utilization Gene Cluster in Colwellia echini Encodes Enzymes for the Complete Degradation of kappa-Carrageenan, iota-Carrageenan, and Hybrid beta/kappa-Carrageenan. A Novel Auxiliary Agarolytic Pathway Expands Metabolic Versatility in the Agar-Degrading Marine Bacterium Colwellia echini A3(T). mSphere. 2020 Jan 8;5(1):e00792-19. doi: 10.1128/mSphere.00792-19. Appl Environ Microbiol. 2021 May 26;87(12):e0023021. doi: 10.1128/AEM.00230-21. Epub 2021 May 26. |
2020 Jan 8,2021 May 26 | degradation | 45 | 9 | GH117, GH117, GH2, GH29, GH50, GH86, GH96 |
| PUL0483 | growth assay | pectin | Flavobacterium johnsoniae | 19717629 Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis. Appl Environ Microbiol. 2009 Nov;75(21):6864-75. doi: 10.1128/AEM.01495-09. Epub 2009 Aug 28. |
2009 Nov | degradation | 12 | 7 | CE12, CE12, CE20, GH105, GH106, GH117, GH2, GH28 |
| PUL0607 | enzyme activity assay, clone and expression, liquid chromatography and mass spectrometry | agarose | Wenyingzhuangia fucanilytica strain CZ1127 | 32520542 Characterization of a Novel Porphyranase Accommodating Methyl-galactoses at Its Subsites. J Agric Food Chem. 2020 Jul 1;68(26):7032-7039. doi: 10.1021/acs.jafc.0c02404. Epub 2020 Jun 22. |
2020 Jul 1 | degradation | 22 | 8 | GH105, GH154, GH117, GH141, GH16_11, GH16_14, GH2, GH29 |
| PUL0651 | enzyme activity assay, NMR | agarose | Gilvimarinus chinensis DSM 19667 | 33691998 Agarase cocktail from agar polysaccharide utilization loci converts homogenized Gelidium amansii into neoagarooligosaccharides. Food Chem. 2021 Aug 1;352:128685. doi: 10.1016/j.foodchem.2020.128685. Epub 2020 Nov 19. |
2021 Aug 1 | degradation | 63 | 15 | CBM6, CBM6, CBM6, GH86, GH86, CE1, GH117, GH127, GH16_16, CBM13, GH16_16, CBM6, CBM6, GH16_3, GH167, GH2, GH50, GH86 |
| PUL0653 | gene deletion mutant and growth assay, complementation study, enzyme activity assay, RNA-seq, electrophoretic mobility shift assay | agarose | Streptomyces coelicolor A3(2) | 33889146 LacI-Family Transcriptional Regulator DagR Acts as a Repressor of the Agarolytic Pathway Genes in Streptomyces coelicolor A3(2). Front Microbiol. 2021 Apr 6;12:658657. doi: 10.3389/fmicb.2021.658657. eCollection 2021. |
2021 | degradation | 17 | 4 | GH117, GH117, GH16_16, GH2, GH50 |
| PUL0703 | enzyme activity assay, recombinant protein expression, thin-layer chromatography | agarose | Aquimarina sp. ERC-38 | 37002465 Agarolytic Pathway in the Newly Isolated Aquimarina sp. Bacterial Strain ERC-38 and Characterization of a Putative beta-agarase. Mar Biotechnol (NY). 2023 Apr;25(2):314-327. doi: 10.1007/s10126-023-10206-7. Epub 2023 Apr 1. |
2023 Apr | degradation | 36 | 10 | CE1, GH117, GH117, GH16_15, GH16_16, GH16_16, CBM6, GH2, GH82, GH86, GH86, GH86, CBM6 |
| PUL0706 | RNA-seq, growth assay | agar | Pseudoalteromonas atlantica T6c | 37265394 Constructing Marine Bacterial Metabolic Chassis for Potential Biorefinery of Red Algal Biomass and Agaropectin Wastes. ACS Synth Biol. 2023 Jun 16;12(6):1782-1793. doi: 10.1021/acssynbio.3c00063. Epub 2023 Jun 2. |
2023 Jun 16 | degradation | 43 | 15 | CE20, CE20, GH117, GH117, GH140, GH16_12, GH16_14, GH2, GH29, GH3, GH43_12, CBM91, GH43_2, CBM6, GH86 |
| PUL0707 | RNA-seq, growth assay | agar | Pseudoalteromonas atlantica T6c | 37265394 Constructing Marine Bacterial Metabolic Chassis for Potential Biorefinery of Red Algal Biomass and Agaropectin Wastes. ACS Synth Biol. 2023 Jun 16;12(6):1782-1793. doi: 10.1021/acssynbio.3c00063. Epub 2023 Jun 2. |
2023 Jun 16 | degradation | 46 | 4 | AA2, GH117, GH117, GH13_13, GH50 |
| PUL0727 | SDS-PAGE, NMR, enzyme activity assay, size-exclusion chromatography (SEC) | fucoidan | Wenyingzhuangia fucanilytica strain CZ1127 | 38203394 The Discovery of the Fucoidan-Active Endo-1-->4-alpha-L-Fucanase of the GH168 Family, Which Produces Fucoidan Derivatives with Regular Sulfation and Anticoagulant Activity. Int J Mol Sci. 2023 Dec 22;25(1):218. doi: 10.3390/ijms25010218. |
2023 Dec 22 | degradation | 30 | 16 | GH107, GH117, GH141, GH168, GH29, GH43_2, GH95 |
| PUL0774 | RNA-seq, differential gene expression, HPAEC-PAD, SDS-PAGE, para-hydroxybenzoic acid (PAHBAH) assay, reducing-sugar assay, Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE), enzyme activity assay | fucoidan | Rhodopirellula sp. SWK7 | 39738071 Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w. |
2024 Dec 30 | degradation | 23 | 8 | GH117, GH141, GH168, GH29, GH95 |
| PUL0776 | RNA-seq, differential gene expression, HPAEC-PAD, SDS-PAGE, para-hydroxybenzoic acid (PAHBAH) assay, reducing-sugar assay, Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE), enzyme activity assay | fucoidan | Rhodopirellula sp. SWK7 | 39738071 Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w. |
2024 Dec 30 | degradation | 32 | 10 | CE19, CBM51, CE20, GH115, GH116, GH117, GH117, GH29, GH95 |
| PUL0782 | RNA-seq, differential gene expression, HPAEC-PAD, SDS-PAGE, para-hydroxybenzoic acid (PAHBAH) assay, reducing-sugar assay, Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE), enzyme activity assay | fucoidan | Neorhodopirellula lusitana | 39738071 Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w. |
2024 Dec 30 | degradation | 15 | 3 | GH109, GH117, GH29 |
| PUL0785 | RNA-seq, differential gene expression, HPAEC-PAD, SDS-PAGE, para-hydroxybenzoic acid (PAHBAH) assay, reducing-sugar assay, Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE), enzyme activity assay | fucoidan | Neorhodopirellula lusitana | 39738071 Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w. |
2024 Dec 30 | degradation | 22 | 5 | CE20, CE20, GH95, CE7, GH117, GH168 |
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