| PULID | Characterization Method(s) | Substrate | Organism | Publication | Publish Date | Type | Num Genes | Num CAZymes | CazyFamily |
|---|---|---|---|---|---|---|---|---|---|
| PUL0001 | RNA-seq, substrate binding assay, enzyme activity assay, mass spectrometry | beta-mannan | Roseburia intestinalis | <a href=https://pubmed.ncbi.nlm.nih.gov/30796211/>30796211</a> The human gut Firmicute Roseburia intestinalis is a primary degrader of dietary beta-mannans. Nat Commun. 2019 Feb 22;10(1):905. doi: 10.1038/s41467-019-08812-y. |
2019 Feb 22 | degradation | 15 | 9 | CE17, CBM35inCE17, CE2, GH1, GH113, GH130_1, GH130_2, GH36 |
| PUL0004 | enzyme activity assay, substrate binding assay | beta-glucan | uncultured bacterium | <a href=https://pubmed.ncbi.nlm.nih.gov/26827771/>26827771</a> A novel metagenome-derived gene cluster from termite hindgut: Encoding phosphotransferase system components and high glucose tolerant glucosidase. Enzyme Microb Technol. 2016 Mar;84:24-31. doi: 10.1016/j.enzmictec.2015.12.005. Epub 2015 Dec 15. |
2016 Mar | degradation | 2 | 1 | GH1 |
| PUL0006 | enzyme activity assay | galactan | Geobacillus stearothermophilus | <a href=https://pubmed.ncbi.nlm.nih.gov/24637762/>24637762</a> Purification, crystallization and preliminary crystallographic analysis of Gan1D, a GH1 6-phospho-beta-galactosidase from Geobacillus stearothermophilus T1. Acta Crystallogr F Struct Biol Commun. 2014 Feb;70(Pt 2):225-31. doi: 10.1107/S2053230X13034778. Epub 2014 Jan 21. |
2014 Feb | degradation | 10 | 1 | GH1 |
| PUL0016 | microarray | cellobiose | Lactococcus lactis | <a href=https://pubmed.ncbi.nlm.nih.gov/28970222/>28970222</a> Disruption of a Transcriptional Repressor by an Insertion Sequence Element Integration Leads to Activation of a Novel Silent Cellobiose Transporter in Lactococcus lactis MG1363. Appl Environ Microbiol. 2017 Nov 16;83(23):e01279-17. doi: 10.1128/AEM.01279-17. Print 2017 Dec 1. |
2017 Dec 1 | degradation | 5 | 1 | GH1 |
| PUL0022 | RT-PCR, gene deletion mutant and growth assay, enzyme activity assay | cellobiose | Bacillus coagulans | <a href=https://pubmed.ncbi.nlm.nih.gov/30519284/>30519284</a> Simultaneous consumption of cellobiose and xylose by Bacillus coagulans to circumvent glucose repression and identification of its cellobiose-assimilating operons. Biotechnol Biofuels. 2018 Dec 1;11:320. doi: 10.1186/s13068-018-1323-5. eCollection 2018. |
2018 | degradation | 6 | 1 | GH1 |
| PUL0023 | RT-PCR, gene deletion mutant and growth assay, enzyme activity assay | cellobiose | Bacillus coagulans | <a href=https://pubmed.ncbi.nlm.nih.gov/30519284/>30519284</a> Simultaneous consumption of cellobiose and xylose by Bacillus coagulans to circumvent glucose repression and identification of its cellobiose-assimilating operons. Biotechnol Biofuels. 2018 Dec 1;11:320. doi: 10.1186/s13068-018-1323-5. eCollection 2018. |
2018 | degradation | 5 | 1 | GH1 |
| PUL0028 | microarray, qPCR, enzyme activity assay | mucin | [Ruminococcus] gnavus | <a href=https://pubmed.ncbi.nlm.nih.gov/24204617/>24204617</a> Utilisation of mucin glycans by the human gut symbiont Ruminococcus gnavus is strain-dependent. PLoS One. 2013 Oct 25;8(10):e76341. doi: 10.1371/journal.pone.0076341. eCollection 2013. |
2013 | degradation | 14 | 4 | CBM40, GH33, GH1, GH140, GH177 |
| PUL0041 | Southern Blot, enzyme activity assay | cellobiose | Klebsiella oxytoca | <a href=https://pubmed.ncbi.nlm.nih.gov/9023916/>9023916</a> Cloning of cellobiose phosphoenolpyruvate-dependent phosphotransferase genes: functional expression in recombinant Escherichia coli and identification of a putative binding region for disaccharides. Appl Environ Microbiol. 1997 Feb;63(2):355-63. doi: 10.1128/aem.63.2.355-363.1997. |
1997 Feb | degradation | 3 | 1 | GH1 |
| PUL0107 | fosmid library screen, lectin binding assay | host glycan | uncultured bacterium | <a href=https://pubmed.ncbi.nlm.nih.gov/31275257/>31275257</a> Investigating Host Microbiota Relationships Through Functional Metagenomics. Front Microbiol. 2019 Jun 7;10:1286. doi: 10.3389/fmicb.2019.01286. eCollection 2019. |
2019 | degradation | 27 | 1 | GH1 |
| PUL0113 | sequence homology analysis | host glycan | Faecalibacterium prausnitzii | <a href=https://pubmed.ncbi.nlm.nih.gov/31275257/>31275257</a> Investigating Host Microbiota Relationships Through Functional Metagenomics. Front Microbiol. 2019 Jun 7;10:1286. doi: 10.3389/fmicb.2019.01286. eCollection 2019. |
2019 | degradation | 23 | 1 | GH1 |
| PUL0144 | enzyme activity assay, Western Blot | chitin | Thermococcus kodakarensis | <a href=https://pubmed.ncbi.nlm.nih.gov/16199574/>16199574</a> Characterization of a novel glucosamine-6-phosphate deaminase from a hyperthermophilic archaeon. J Bacteriol. 2005 Oct;187(20):7038-44. doi: 10.1128/JB.187.20.7038-7044.2005. |
2005 Oct | degradation | 12 | 4 | CE14, GH1, GH18, GH35 |
| PUL0153 | RNA-seq | human milk oligosaccharide | Lactobacillus rhamnosus | <a href=https://pubmed.ncbi.nlm.nih.gov/30332787/>30332787</a> Prebiotics for Lactose Intolerance: Variability in Galacto-Oligosaccharide Utilization by Intestinal Lactobacillus rhamnosus. Nutrients. 2018 Oct 16;10(10):1517. doi: 10.3390/nu10101517. |
2018 Oct 16 | degradation | 3 | 1 | GH1 |
| PUL0154 | RNA-seq, differential gene expression | human milk oligosaccharide | Lactobacillus rhamnosus | <a href=https://pubmed.ncbi.nlm.nih.gov/30332787/>30332787</a> Prebiotics for Lactose Intolerance: Variability in Galacto-Oligosaccharide Utilization by Intestinal Lactobacillus rhamnosus. Nutrients. 2018 Oct 16;10(10):1517. doi: 10.3390/nu10101517. |
2018 Oct 16 | degradation | 3 | 1 | GH1 |
| PUL0157 | RNA-seq, differential gene expression | human milk oligosaccharide | Lactobacillus rhamnosus | <a href=https://pubmed.ncbi.nlm.nih.gov/30332787/>30332787</a> Prebiotics for Lactose Intolerance: Variability in Galacto-Oligosaccharide Utilization by Intestinal Lactobacillus rhamnosus. Nutrients. 2018 Oct 16;10(10):1517. doi: 10.3390/nu10101517. |
2018 Oct 16 | degradation | 4 | 1 | GH1 |
| PUL0158 | RNA-seq, differential gene expression | human milk oligosaccharide | Lactobacillus rhamnosus | <a href=https://pubmed.ncbi.nlm.nih.gov/30332787/>30332787</a> Prebiotics for Lactose Intolerance: Variability in Galacto-Oligosaccharide Utilization by Intestinal Lactobacillus rhamnosus. Nutrients. 2018 Oct 16;10(10):1517. doi: 10.3390/nu10101517. |
2018 Oct 16 | degradation | 4 | 1 | GH1 |
| PUL0186 | gene deletion mutant and growth assay | cellobiose | Streptococcus pneumoniae | <a href=https://pubmed.ncbi.nlm.nih.gov/17028271/>17028271</a> The two-component regulatory system TCS08 is involved in cellobiose metabolism of Streptococcus pneumoniae R6. J Bacteriol. 2007 Feb;189(4):1342-50. doi: 10.1128/JB.01170-06. Epub 2006 Oct 6. |
2007 Feb | degradation | 7 | 1 | GH1 |
| PUL0195 | RT-PCR | cellobiose | Clostridium acetobutylicum | <a href=https://pubmed.ncbi.nlm.nih.gov/26691835/>26691835</a> PTS regulation domain-containing transcriptional activator CelR and sigma factor sigma(54) control cellobiose utilization in Clostridium acetobutylicum. Mol Microbiol. 2016 Apr;100(2):289-302. doi: 10.1111/mmi.13316. Epub 2016 Feb 9. |
2016 Apr | degradation | 5 | 1 | GH1 |
| PUL0231 | enzyme activity assay, cosmid library screening | beta-glucoside | Pectobacterium carotovorum subsp. carotovorum | <a href=https://pubmed.ncbi.nlm.nih.gov/22502871/>22502871</a> Cloning and biochemical analysis of beta-glucoside utilization (bgl) operon without phosphotransferase system in Pectobacterium carotovorum subsp. carotovorum LY34. Microbiol Res. 2012 Sep 6;167(8):461-9. doi: 10.1016/j.micres.2012.03.004. Epub 2012 Apr 12. |
2012 Sep 6 | degradation | 2 | 1 | GH1 |
| PUL0238 | Northern Blot | glucomannan | Bacillus subtilis | <a href=https://pubmed.ncbi.nlm.nih.gov/18177310/>18177310</a> Glucomannan utilization operon of Bacillus subtilis. FEMS Microbiol Lett. 2008 Feb;279(1):103-9. doi: 10.1111/j.1574-6968.2007.01018.x. |
2008 Feb | degradation | 8 | 2 | GH1, GH26 |
| PUL0321 | enzyme activity assay, transposon mutagenesis | beta-glucoside | Escherichia coli | <a href=https://pubmed.ncbi.nlm.nih.gov/19233952/>19233952</a> Characterization of a beta-glucoside operon (bgc) prevalent in septicemic and uropathogenic Escherichia coli strains. Appl Environ Microbiol. 2009 Apr;75(8):2284-93. doi: 10.1128/AEM.02621-08. Epub 2009 Feb 20. |
2009 Apr | degradation | 6 | 1 | GH1 |
| PUL0331 | carbon utilization assay | cellobiose | Aliivibrio fischeri | <a href=https://pubmed.ncbi.nlm.nih.gov/18487409/>18487409</a> Identification of a cellobiose utilization gene cluster with cryptic beta-galactosidase activity in Vibrio fischeri. Appl Environ Microbiol. 2008 Jul;74(13):4059-69. doi: 10.1128/AEM.00190-08. Epub 2008 May 16. |
2008 Jul | degradation | 6 | 1 | GH1 |
| PUL0370 | RT-PCR | beta-glucoside | Corynebacterium glutamicum | <a href=https://pubmed.ncbi.nlm.nih.gov/19628558/>19628558</a> Identification of a second beta-glucoside phosphoenolpyruvate: carbohydrate phosphotransferase system in Corynebacterium glutamicum R. Microbiology (Reading). 2009 Nov;155(Pt 11):3652-3660. doi: 10.1099/mic.0.029496-0. Epub 2009 Jul 23. |
2009 Nov | degradation | 5 | 1 | GH1 |
| PUL0372 | enzyme activity assay | beta-glucoside | Corynebacterium glutamicum | <a href=https://pubmed.ncbi.nlm.nih.gov/19628558/>19628558</a> Identification of a second beta-glucoside phosphoenolpyruvate: carbohydrate phosphotransferase system in Corynebacterium glutamicum R. Microbiology (Reading). 2009 Nov;155(Pt 11):3652-3660. doi: 10.1099/mic.0.029496-0. Epub 2009 Jul 23. |
2009 Nov | degradation | 3 | 1 | GH1 |
| PUL0401 | RNA-seq | beta-glucan | Alteromonas macleodii | <a href=https://pubmed.ncbi.nlm.nih.gov/30116038/>30116038</a> Biphasic cellular adaptations and ecological implications of Alteromonas macleodii degrading a mixture of algal polysaccharides. ISME J. 2019 Jan;13(1):92-103. doi: 10.1038/s41396-018-0252-4. Epub 2018 Aug 16. |
2019 Jan | degradation | 9 | 3 | GH1, GH16_3, GH3 |
| PUL0403 | RNA-seq | beta-glucan | Alteromonas macleodii | <a href=https://pubmed.ncbi.nlm.nih.gov/30116038/>30116038</a> Biphasic cellular adaptations and ecological implications of Alteromonas macleodii degrading a mixture of algal polysaccharides. ISME J. 2019 Jan;13(1):92-103. doi: 10.1038/s41396-018-0252-4. Epub 2018 Aug 16. |
2019 Jan | degradation | 4 | 1 | GH1 |
| PUL0407 | primer extension analysis, enzyme activity assay | human milk oligosaccharide | Lactobacillus casei | <a href=https://pubmed.ncbi.nlm.nih.gov/9066115/>9066115</a> Establishing a model to study the regulation of the lactose operon in Lactobacillus casei. FEMS Microbiol Lett. 1997 Mar 1;148(1):83-9. doi: 10.1111/j.1574-6968.1997.tb10271.x. |
1997 Mar 1 | degradation | 4 | 1 | GH1 |
| PUL0413 | enzyme activity assay, reducing-sugar assay | cellobiose | uncultured bacterium contig00059 | <a href=https://pubmed.ncbi.nlm.nih.gov/30116044/>30116044</a> Functional metagenomics reveals abundant polysaccharide-degrading gene clusters and cellobiose utilization pathways within gut microbiota of a wood-feeding higher termite. ISME J. 2019 Jan;13(1):104-117. doi: 10.1038/s41396-018-0255-1. Epub 2018 Aug 16. |
2019 Jan | degradation | 31 | 2 | GH1, GH44 |
| PUL0414 | enzyme activity assay, thin-layer chromatography | xylan | uncultured bacterium 35A20 | <a href=https://pubmed.ncbi.nlm.nih.gov/30116044/>30116044</a> Functional metagenomics reveals abundant polysaccharide-degrading gene clusters and cellobiose utilization pathways within gut microbiota of a wood-feeding higher termite. ISME J. 2019 Jan;13(1):104-117. doi: 10.1038/s41396-018-0255-1. Epub 2018 Aug 16. |
2019 Jan | degradation | 25 | 4 | GH1, GH10 |
| PUL0423 | clone and expression, enzyme activity assay | cellobiose | Thermotoga neapolitana | <a href=https://pubmed.ncbi.nlm.nih.gov/10960102/>10960102</a> Cloning and characterization of the glucooligosaccharide catabolic pathway beta-glucan glucohydrolase and cellobiose phosphorylase in the marine hyperthermophile Thermotoga neapolitana. J Bacteriol. 2000 Sep;182(18):5172-9. doi: 10.1128/JB.182.18.5172-5179.2000. |
2000 Sep | degradation | 3 | 2 | GH1, GH94 |
| PUL0475 | clone and expression, gene deletion mutant and growth assay | beta-glucan | Streptomyces reticuli | <a href=https://pubmed.ncbi.nlm.nih.gov/10347054/>10347054</a> Characterization of the binding protein-dependent cellobiose and cellotriose transport system of the cellulose degrader Streptomyces reticuli. Appl Environ Microbiol. 1999 Jun;65(6):2636-43. doi: 10.1128/AEM.65.6.2636-2643.1999. |
1999 Jun | degradation | 7 | 2 | CBM2, GH18, GH1 |
| PUL0531 | clone and expression, enzyme activity assay | chitin | Serratia marcescens | <a href=https://pubmed.ncbi.nlm.nih.gov/12618440/>12618440</a> Uptake of N,N'-diacetylchitobiose [(GlcNAc)2] via the phosphotransferase system is essential for chitinase production by Serratia marcescens 2170. J Bacteriol. 2003 Mar;185(6):1776-82. doi: 10.1128/JB.185.6.1776-1782.2003. |
2003 Mar | degradation | 5 | 1 | GH1 |
| PUL0570 | clone and expression, enzyme activity assay | cellobiose | Corynebacterium glutamicum | <a href=https://pubmed.ncbi.nlm.nih.gov/12777497/>12777497</a> A single V317A or V317M substitution in Enzyme II of a newly identified beta-glucoside phosphotransferase and utilization system of Corynebacterium glutamicum R extends its specificity towards cellobiose. Microbiology (Reading). 2003 Jun;149(Pt 6):1569-1580. doi: 10.1099/mic.0.26053-0. |
2003 Jun | degradation | 3 | 1 | GH1 |
| PUL0573 | enzyme activity assay, electrophoretic mobility shift assay, RT-PCR, qRT-PCR | beta-glucan | Streptomyces griseus | <a href=https://pubmed.ncbi.nlm.nih.gov/19648249/>19648249</a> CebR as a master regulator for cellulose/cellooligosaccharide catabolism affects morphological development in Streptomyces griseus. J Bacteriol. 2009 Oct;191(19):5930-40. doi: 10.1128/JB.00703-09. Epub 2009 Jul 31. |
2009 Oct | degradation | 5 | 1 | GH1 |
| PUL0574 | enzyme activity assay | alpha-mannan | Streptococcus pyogenes | <a href=https://pubmed.ncbi.nlm.nih.gov/16822234/>16822234</a> Functional analysis of a group A streptococcal glycoside hydrolase Spy1600 from family 84 reveals it is a beta-N-acetylglucosaminidase and not a hyaluronidase. Biochem J. 2006 Oct 15;399(2):241-7. doi: 10.1042/BJ20060307. |
2006 Oct 15 | degradation | 8 | 4 | GH1, GH125, GH38, GH84 |
| PUL0578 | qRT-PCR, enzyme activity assay, electrophoretic mobility shift assay | beta-glucan | Bifidobacterium breve | <a href=https://pubmed.ncbi.nlm.nih.gov/21216899/>21216899</a> Cellodextrin utilization by bifidobacterium breve UCC2003. Appl Environ Microbiol. 2011 Mar;77(5):1681-90. doi: 10.1128/AEM.01786-10. Epub 2011 Jan 7. |
2011 Mar | degradation | 5 | 1 | GH1 |
| PUL0582 | NMR, microarray, enzyme activity assay, gene deletion mutant and growth assay | human milk oligosaccharide | Lactococcus lactis | <a href=https://pubmed.ncbi.nlm.nih.gov/22660716/>22660716</a> A specific mutation in the promoter region of the silent cel cluster accounts for the appearance of lactose-utilizing Lactococcus lactis MG1363. Appl Environ Microbiol. 2012 Aug;78(16):5612-21. doi: 10.1128/AEM.00455-12. Epub 2012 Jun 1. |
2012 Aug | degradation | 5 | 2 | GH1, GH170 |
| PUL0585 | microarray, gene deletion mutant and growth assay, beta-galactosidase assays | cellobiose | Streptococcus pneumoniae | <a href=https://pubmed.ncbi.nlm.nih.gov/21778207/>21778207</a> CelR-mediated activation of the cellobiose-utilization gene cluster in Streptococcus pneumoniae. Microbiology (Reading). 2011 Oct;157(Pt 10):2854-2861. doi: 10.1099/mic.0.051359-0. Epub 2011 Jul 21. |
2011 Oct | degradation | 7 | 1 | GH1 |
| PUL0603 | microarray, qRT-PCR, culureing methods | galactomannan | Lactobacillus plantarum WCFS1 | <a href=https://pubmed.ncbi.nlm.nih.gov/31703861/>31703861</a> Transcriptional analysis of galactomannooligosaccharides utilization by Lactobacillus plantarum WCFS1. Food Microbiol. 2020 Apr;86:103336. doi: 10.1016/j.fm.2019.103336. Epub 2019 Sep 14. |
2020 Apr | degradation | 3 | 1 | GH1 |
| PUL0699 | RT-qPCR, high-performance anion-exchange chromatography | beta-mannan | Roseburia hominis A2-183 | <a href=https://pubmed.ncbi.nlm.nih.gov/36557749/>36557749</a> Cross-Feeding and Enzymatic Catabolism for Mannan-Oligosaccharide Utilization by the Butyrate-Producing Gut Bacterium Roseburia hominis A2-183. Microorganisms. 2022 Dec 16;10(12):2496. doi: 10.3390/microorganisms10122496. |
2022 Dec 16 | degradation | 14 | 7 | CE17, CBM35inCE17, CE2, GH1, GH130_1, GH130_2, GH3, GH36 |
| PUL0744 | RNA-seq, HPLC, gene mutant, differential gene expression | lactose | Listeria monocytogenes serotype 4b str. F2365 | <a href=https://pubmed.ncbi.nlm.nih.gov/38876592/>38876592</a> Activation of a silent lactose utilization pathway in an evolved Listeria monocytogenes F2365 outbreak isolate. Food Res Int. 2024 Aug;189:114554. doi: 10.1016/j.foodres.2024.114554. Epub 2024 May 27. |
2024 Aug | degradation | 5 | 1 | GH1 |
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