Browse dbCAN-PUL Entries
| PULID | Characterization Method(s) | Substrate | Organism | Publication | Publish Date | Type | Num Genes | Num CAZymes | CazyFamily |
|---|---|---|---|---|---|---|---|---|---|
| PUL0086 | label-free quantitative proteomics, functional enrichment analysis, enzyme activity assay | pectin | Ruminiclostridium papyrosolvens | 31338125 Secretomic analyses of Ruminiclostridium papyrosolvens reveal its enzymatic basis for lignocellulose degradation. Biotechnol Biofuels. 2019 Jul 15;12:183. doi: 10.1186/s13068-019-1522-8. eCollection 2019. |
2019 | degradation | 3 | 3 | CE8, PL10_1, PL11 |
| PUL0135 | enzyme activity assay, carbohydrate binding assay | pectin | Pseudoalteromonas sp. | 30341080 Biochemical Reconstruction of a Metabolic Pathway from a Marine Bacterium Reveals Its Mechanism of Pectin Depolymerization. Appl Environ Microbiol. 2018 Dec 13;85(1):e02114-18. doi: 10.1128/AEM.02114-18. Print 2019 Jan 1. |
2019 Jan 1 | degradation | 21 | 7 | CE12, CE8, GH105, GH28, GH43_10, CBM91, PL1_2 |
| PUL0136 | sequence homology analysis | pectin | Pseudoalteromonas haloplanktis | 30341080 Biochemical Reconstruction of a Metabolic Pathway from a Marine Bacterium Reveals Its Mechanism of Pectin Depolymerization. Appl Environ Microbiol. 2018 Dec 13;85(1):e02114-18. doi: 10.1128/AEM.02114-18. Print 2019 Jan 1. |
2019 Jan 1 | degradation | 26 | 6 | CE12, CE8, PL1_5, GH105, GH28, PL1_2 |
| PUL0191 | RNA-seq, RT-PCR, qPCR | pectin | Bacteroides xylanisolvens | 26920945 Unraveling the pectinolytic function of Bacteroides xylanisolvens using a RNA-seq approach and mutagenesis. BMC Genomics. 2016 Feb 27;17:147. doi: 10.1186/s12864-016-2472-1. |
2016 Feb 27 | degradation | 11 | 5 | CE12, CE8, CE8, GH105, PL1_2 |
| PUL0305 | RT-qPCR, isothermal titration calorimetry (ITC), enzyme activity assay, gene deletion mutant and growth assay | pectin | Bacteroides thetaiotaomicron | 29255254 Dietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides. Nat Microbiol. 2018 Feb;3(2):210-219. doi: 10.1038/s41564-017-0079-1. Epub 2017 Dec 18. |
2018 Feb | degradation | 17 | 7 | CE12, CE8, CE8, GH105, GH28, PL1_2 |
| PUL0330 | fosmid library screen, enzyme activity assay, thin-layer chromatography | pectin | Gramella flava | 28261179 Characterization of Potential Polysaccharide Utilization Systems in the Marine Bacteroidetes Gramella Flava JLT2011 Using a Multi-Omics Approach. Biochemical Reconstruction of a Metabolic Pathway from a Marine Bacterium Reveals Its Mechanism of Pectin Depolymerization. Front Microbiol. 2017 Feb 14;8:220. doi: 10.3389/fmicb.2017.00220. eCollection 2017. Appl Environ Microbiol. 2018 Dec 13;85(1):e02114-18. doi: 10.1128/AEM.02114-18. Print 2019 Jan 1. |
2017,2019 Jan 1 | degradation | 28 | 10 | CE12, CE8, GH105, GH28, GH28, PL9_1, GH43_10, CBM91, PL10_1, PL9_1 |
| PUL0363 | enzyme activity assay | pectin | Xanthomonas campestris pv. campestris | 17311090 Plant carbohydrate scavenging through tonB-dependent receptors: a feature shared by phytopathogenic and aquatic bacteria. PLoS One. 2007 Feb 21;2(2):e224. doi: 10.1371/journal.pone.0000224. |
2007 Feb 21 | degradation | 3 | 2 | CE8, PL10_1 |
| PUL0404 | RNA-seq | pectin | Alteromonas macleodii | 30116038 Biphasic cellular adaptations and ecological implications of Alteromonas macleodii degrading a mixture of algal polysaccharides. ISME J. 2019 Jan;13(1):92-103. doi: 10.1038/s41396-018-0252-4. Epub 2018 Aug 16. |
2019 Jan | degradation | 18 | 4 | CE12, CE8, GH105, GH28 |
| PUL0482 | growth assay | pectin | Flavobacterium johnsoniae | 19717629 Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis. Appl Environ Microbiol. 2009 Nov;75(21):6864-75. doi: 10.1128/AEM.01495-09. Epub 2009 Aug 28. |
2009 Nov | degradation | 27 | 21 | CBM67, GH78, CE19, CE20, CE8, GH106, GH127, GH137, GH139, GH140, GH142, GH143, GH2, GH28, GH43_18, GH78, GH95, PL1_2, PL29 |
| PUL0484 | growth assay | pectin | Flavobacterium johnsoniae | 19717629 Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis. Appl Environ Microbiol. 2009 Nov;75(21):6864-75. doi: 10.1128/AEM.01495-09. Epub 2009 Aug 28. |
2009 Nov | degradation | 12 | 8 | CE12, GH105, GH28, GH43_10, CBM91, PL10_1, CE8 |
| PUL0529 | microarray, qPCR, RNA-seq, reducing-sugar assay, growth assay | pectin | Bacteroides ovatus | 22205877 Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. In vitro fermentation of a purified fraction of polysaccharides from the root of Brassica rapa L. by human gut microbiota and its interaction with Bacteroides ovatus. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. Food Chem. 2025 May 1;473:143109. doi: 10.1016/j.foodchem.2025.143109. Epub 2025 Jan 27. |
2011 Dec,2025 May 1 | degradation | 27 | 13 | CE12, CE8, CE8, GH105, GH28, GH3, GH43_10, CBM91, PL1_2 |
| PUL0550 | RNA-seq | glycosaminoglycan | Bacteroides cellulosilyticus | 23976882 Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20. |
2013 | degradation | 7 | 3 | CE8, GH3, PL1_2 |
| PUL0739 | mass spectrometry, SDS-PAGE, growth assay | arabinogalactan | Maribacter sp. MAR_2009_72 | 38569650 Proteomic insight into arabinogalactan utilization by particle-associated Maribacter sp. MAR_2009_72. FEMS Microbiol Ecol. 2024 Apr 10;100(5):fiae045. doi: 10.1093/femsec/fiae045. |
2024 Apr 10 | degradation | 56 | 12 | CE12, GH105, GH140, GH177, GH179, GH28, GH43_10, CBM91, GH43_19, GH43_34, GH51_1, PL10_1, CE8, PL1_2 |
| PUL0767 | RNA-seq, qRT-PCR, gas chromatography, mass spectrometry, thin-layer chromatography | pectin | Segatella copri DSM 18205 | 39636128 Transcriptional delineation of polysaccharide utilization loci in the human gut commensal Segatella copri DSM18205 and co-culture with exemplar Bacteroides species on dietary plant glycans. Appl Environ Microbiol. 2025 Jan 31;91(1):e0175924. doi: 10.1128/aem.01759-24. Epub 2024 Dec 5. |
2025 Jan 31 | degradation | 15 | 6 | CE8, GH28, GH28, GH105, GH43_10, CBM91, GH95, PL1_2 |
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