| PULID | Characterization Method(s) | Substrate | Organism | Publication | Publish Date | Type | Num Genes | Num CAZymes | CazyFamily |
|---|---|---|---|---|---|---|---|---|---|
| PUL0030 | isothermal calorimetric titration, gene deletion mutant and growth assay, enzyme activity assay | galactomannan | Bacillus sp. N16-5 | <a href=https://pubmed.ncbi.nlm.nih.gov/26978267/>26978267</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/30351049/>30351049</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/29360976/>29360976</a> A Novel Manno-Oligosaccharide Binding Protein Identified in Alkaliphilic Bacillus sp. N16-5 Is Involved in Mannan Utilization. Galactomannan Degrading Enzymes from the Mannan Utilization Gene Cluster of Alkaliphilic Bacillus sp. N16-5 and Their Synergy on Galactomannan Degradation. Transcriptional regulation of the mannan utilization genes in the alkaliphilic Bacillus sp. N16-5. PLoS One. 2016 Mar 15;11(3):e0150059. doi: 10.1371/journal.pone.0150059. eCollection 2016. J Agric Food Chem. 2018 Oct 24;66(42):11055-11063. doi: 10.1021/acs.jafc.8b03878. Epub 2018 Oct 15. FEMS Microbiol Lett. 2018 Feb 1;365(4). doi: 10.1093/femsle/fnx280. |
2016,2018 Oct 24,2018 Feb 1 | degradation | 12 | 6 | CE7, GH130_1, GH130_2, GH27 |
| PUL0129 | enzyme activity assay | beta-mannan | gut metagenome | <a href=https://pubmed.ncbi.nlm.nih.gov/30356154/>30356154</a> Interspecies cross-feeding orchestrates carbon degradation in the rumen ecosystem. Nat Microbiol. 2018 Nov;3(11):1274-1284. doi: 10.1038/s41564-018-0225-4. Epub 2018 Oct 24. |
2018 Nov | degradation | 12 | 6 | CE7, GH130_1, GH26, GH5_4 |
| PUL0179 | enzyme activity assay, enzyme specificity assay, substrate specificity assay | galactomannan | Bacteroides ovatus | <a href=https://pubmed.ncbi.nlm.nih.gov/27288925/>27288925</a> A beta-mannan utilization locus in Bacteroides ovatus involves a GH36 alpha-galactosidase active on galactomannans. FEBS Lett. 2016 Jul;590(14):2106-18. doi: 10.1002/1873-3468.12250. Epub 2016 Jun 28. |
2016 Jul | degradation | 15 | 4 | CE7, GH130_1, GH26 |
| PUL0239 | fosmid library screen, sequence homology analysis | cellulose | Prevotella sp. Sc00026 | <a href=https://pubmed.ncbi.nlm.nih.gov/24448980/>24448980</a> Analysis of the bovine rumen microbiome reveals a diversity of Sus-like polysaccharide utilization loci from the bacterial phylum Bacteroidetes. J Ind Microbiol Biotechnol. 2014 Mar;41(3):601-6. doi: 10.1007/s10295-013-1395-y. Epub 2014 Jan 22. |
2014 Mar | degradation | 16 | 9 | CE20, CE7, GH130_1, GH26, GH26, GH5_4, GH3, GH36, GH5_7 |
| PUL0332 | fosmid library screen, enzyme activity assay, thin-layer chromatography | beta-glucan | uncultured bacterium | <a href=https://pubmed.ncbi.nlm.nih.gov/28091525/>28091525</a> A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 19 | 8 | CE7, GH127, GH2, GH5_2, GH5_7, GH94, GH97 |
| PUL0390 | enzyme activity assay | xylan | Thermotoga maritima | <a href=https://pubmed.ncbi.nlm.nih.gov/21255309/>21255309</a> Hyperthermostable acetyl xylan esterase. Microb Biotechnol. 2010 Jan;3(1):84-92. doi: 10.1111/j.1751-7915.2009.00150.x. Epub 2009 Sep 18. |
2010 Jan | degradation | 24 | 6 | CBM22, CBM22, CBM22, GH10, CBM9, CBM9, CE7, GH10, GH3, GH67 |
| PUL0538 | RNA-seq | galactomannan | Bacteroides cellulosilyticus | <a href=https://pubmed.ncbi.nlm.nih.gov/23976882/>23976882</a> Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20. |
2013 | degradation | 15 | 7 | CE7, GH130_1, GH26, GH26, GH26, GH3, GH5_2, GH5_7 |
| PUL0662 | thin-layer chromatography, liquid chromatography and mass spectrometry, qPCR, clone and expression | beta-mannan | Phocaeicola dorei DSM 17855 | <a href=https://pubmed.ncbi.nlm.nih.gov/34339781/>34339781</a> BdPUL12 depolymerizes beta-mannan-like glycans into mannooligosaccharides and mannose, which serve as carbon sources for Bacteroides dorei and gut probiotics. Int J Biol Macromol. 2021 Sep 30;187:664-674. doi: 10.1016/j.ijbiomac.2021.07.172. Epub 2021 Jul 31. |
2021 Sep 30 | degradation | 8 | 4 | CE7, GH130_1, GH26, GH5_7 |
| PUL0785 | RNA-seq, differential gene expression, HPAEC-PAD, SDS-PAGE, para-hydroxybenzoic acid (PAHBAH) assay, reducing-sugar assay, Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE), enzyme activity assay | fucoidan | Neorhodopirellula lusitana | <a href=https://pubmed.ncbi.nlm.nih.gov/39738071/>39738071</a> Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w. |
2024 Dec 30 | degradation | 22 | 5 | CE20, CE20, GH95, CE7, GH117, GH168 |
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