| PULID | Characterization Method(s) | Substrate | Organism | Publication | Publish Date | Type | Num Genes | Num CAZymes | CazyFamily |
|---|---|---|---|---|---|---|---|---|---|
| PUL0030 | isothermal calorimetric titration, gene deletion mutant and growth assay, enzyme activity assay | galactomannan | Bacillus sp. N16-5 | <a href=https://pubmed.ncbi.nlm.nih.gov/26978267/>26978267</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/30351049/>30351049</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/29360976/>29360976</a> A Novel Manno-Oligosaccharide Binding Protein Identified in Alkaliphilic Bacillus sp. N16-5 Is Involved in Mannan Utilization. Galactomannan Degrading Enzymes from the Mannan Utilization Gene Cluster of Alkaliphilic Bacillus sp. N16-5 and Their Synergy on Galactomannan Degradation. Transcriptional regulation of the mannan utilization genes in the alkaliphilic Bacillus sp. N16-5. PLoS One. 2016 Mar 15;11(3):e0150059. doi: 10.1371/journal.pone.0150059. eCollection 2016. J Agric Food Chem. 2018 Oct 24;66(42):11055-11063. doi: 10.1021/acs.jafc.8b03878. Epub 2018 Oct 15. FEMS Microbiol Lett. 2018 Feb 1;365(4). doi: 10.1093/femsle/fnx280. |
2016,2018 Oct 24,2018 Feb 1 | degradation | 12 | 6 | CE7, GH130_1, GH130_2, GH27 |
| PUL0175 | enzyme activity assay | galactomannan | Cellvibrio mixtus | <a href=https://pubmed.ncbi.nlm.nih.gov/16842369/>16842369</a> Galactomannan hydrolysis and mannose metabolism in Cellvibrio mixtus. FEMS Microbiol Lett. 2006 Aug;261(1):123-32. doi: 10.1111/j.1574-6968.2006.00342.x. |
2006 Aug | degradation | 4 | 3 | GH130_1, GH27, GH5_7 |
| PUL0178 | enzyme activity assay, enzyme specificity assay, substrate specificity assay | galactomannan | Bacteroides ovatus | <a href=https://pubmed.ncbi.nlm.nih.gov/27288925/>27288925</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/22205877/>22205877</a> A beta-mannan utilization locus in Bacteroides ovatus involves a GH36 alpha-galactosidase active on galactomannans. Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. FEBS Lett. 2016 Jul;590(14):2106-18. doi: 10.1002/1873-3468.12250. Epub 2016 Jun 28. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. |
2016 Jul,2011 Dec | degradation | 14 | 4 | GH130_1, GH26, GH36 |
| PUL0179 | enzyme activity assay, enzyme specificity assay, substrate specificity assay | galactomannan | Bacteroides ovatus | <a href=https://pubmed.ncbi.nlm.nih.gov/27288925/>27288925</a> A beta-mannan utilization locus in Bacteroides ovatus involves a GH36 alpha-galactosidase active on galactomannans. FEBS Lett. 2016 Jul;590(14):2106-18. doi: 10.1002/1873-3468.12250. Epub 2016 Jun 28. |
2016 Jul | degradation | 15 | 4 | CE7, GH130_1, GH26 |
| PUL0180 | enzyme activity assay | galactomannan | Bacteroides fragilis | <a href=https://pubmed.ncbi.nlm.nih.gov/27288925/>27288925</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/21539815/>21539815</a> A beta-mannan utilization locus in Bacteroides ovatus involves a GH36 alpha-galactosidase active on galactomannans. New microbial mannan catabolic pathway that involves a novel mannosylglucose phosphorylase. FEBS Lett. 2016 Jul;590(14):2106-18. doi: 10.1002/1873-3468.12250. Epub 2016 Jun 28. Biochem Biophys Res Commun. 2011 May 20;408(4):701-6. doi: 10.1016/j.bbrc.2011.04.095. Epub 2011 Apr 24. |
2016 Jul,2011 May 20 | degradation | 19 | 3 | GH29, GH36 |
| PUL0217 | RNA-seq | galactomannan | Caldanaerobius polysaccharolyticus | <a href=https://pubmed.ncbi.nlm.nih.gov/25342756/>25342756</a> Structural and biochemical basis for mannan utilization by Caldanaerobius polysaccharolyticus strain ATCC BAA-17. J Biol Chem. 2014 Dec 12;289(50):34965-77. doi: 10.1074/jbc.M114.579904. Epub 2014 Oct 23. |
2014 Dec 12 | degradation | 7 | 2 | GH130_2, GH5_36 |
| PUL0538 | RNA-seq | galactomannan | Bacteroides cellulosilyticus | <a href=https://pubmed.ncbi.nlm.nih.gov/23976882/>23976882</a> Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20. |
2013 | degradation | 15 | 7 | CE7, GH130_1, GH26, GH26, GH26, GH3, GH5_2, GH5_7 |
| PUL0565 | microarray, qPCR | galactomannan | Bacteroides ovatus | <a href=https://pubmed.ncbi.nlm.nih.gov/22205877/>22205877</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/27872187/>27872187</a> Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. Galactomannan Catabolism Conferred by a Polysaccharide Utilization Locus of Bacteroides ovatus: ENZYME SYNERGY AND CRYSTAL STRUCTURE OF A beta-MANNANASE. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. J Biol Chem. 2017 Jan 6;292(1):229-243. doi: 10.1074/jbc.M116.746438. Epub 2016 Nov 21. |
2011 Dec,2017 Jan 6 | degradation | 10 | 4 | GH130_1, GH26, GH36 |
| PUL0600 | liquid chromatography and mass spectrometry, differential gene expression | galactomannan | Clostridium cellulovorans | <a href=https://pubmed.ncbi.nlm.nih.gov/26020016/>26020016</a> Elucidation of the recognition mechanisms for hemicellulose and pectin in Clostridium cellulovorans using intracellular quantitative proteome analysis. AMB Express. 2015 May 23;5:29. doi: 10.1186/s13568-015-0115-6. eCollection 2015. |
2015 | degradation | 12 | 3 | GH130_1, GH130_2, GH2 |
| PUL0603 | microarray, qRT-PCR, culureing methods | galactomannan | Lactobacillus plantarum WCFS1 | <a href=https://pubmed.ncbi.nlm.nih.gov/31703861/>31703861</a> Transcriptional analysis of galactomannooligosaccharides utilization by Lactobacillus plantarum WCFS1. Food Microbiol. 2020 Apr;86:103336. doi: 10.1016/j.fm.2019.103336. Epub 2019 Sep 14. |
2020 Apr | degradation | 3 | 1 | GH1 |
| PUL0604 | microarray, qRT-PCR, culureing methods | galactomannan | Lactobacillus plantarum WCFS1 | <a href=https://pubmed.ncbi.nlm.nih.gov/31703861/>31703861</a> Transcriptional analysis of galactomannooligosaccharides utilization by Lactobacillus plantarum WCFS1. Food Microbiol. 2020 Apr;86:103336. doi: 10.1016/j.fm.2019.103336. Epub 2019 Sep 14. |
2020 Apr | degradation | 5 | 2 | GH13_31, GH32 |
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