| PULID | Characterization Method(s) | Substrate | Organism | Publication | Publish Date | Type | Num Genes | Num CAZymes | CazyFamily |
|---|---|---|---|---|---|---|---|---|---|
| PUL0006 | enzyme activity assay | galactan | Geobacillus stearothermophilus | <a href=https://pubmed.ncbi.nlm.nih.gov/24637762/>24637762</a> Purification, crystallization and preliminary crystallographic analysis of Gan1D, a GH1 6-phospho-beta-galactosidase from Geobacillus stearothermophilus T1. Acta Crystallogr F Struct Biol Commun. 2014 Feb;70(Pt 2):225-31. doi: 10.1107/S2053230X13034778. Epub 2014 Jan 21. |
2014 Feb | degradation | 10 | 1 | GH1 |
| PUL0007 | sequence homology analysis | galactan | Leuconostoc gelidum | <a href=https://pubmed.ncbi.nlm.nih.gov/27274361/>27274361</a> Complete genome sequence of Leuconostoc gelidum subsp. gasicomitatum KG16-1, isolated from vacuum-packaged vegetable sausages. Stand Genomic Sci. 2016 Jun 7;11:40. doi: 10.1186/s40793-016-0164-8. eCollection 2016. |
2016 | degradation | 8 | 2 | GH42, GH53 |
| PUL0137 | sequence homology analysis | galactan | Bifidobacterium animalis subsp. animalis | <a href=https://pubmed.ncbi.nlm.nih.gov/30306201/>30306201</a> Staying alive: growth and survival of Bifidobacterium animalis subsp. animalis under in vitro and in vivo conditions. Appl Microbiol Biotechnol. 2018 Dec;102(24):10645-10663. doi: 10.1007/s00253-018-9413-7. Epub 2018 Oct 10. |
2018 Dec | degradation | 3 | 1 | GH2 |
| PUL0209 | enzyme activity assay, gene deletion mutant and growth assay | galactan | Dickeya dadantii | <a href=https://pubmed.ncbi.nlm.nih.gov/17644603/>17644603</a> Characterization of the Erwinia chrysanthemi Gan locus, involved in galactan catabolism. J Bacteriol. 2007 Oct;189(19):7053-61. doi: 10.1128/JB.00845-07. Epub 2007 Jul 20. |
2007 Oct | degradation | 9 | 2 | GH42, GH53 |
| PUL0282 | enzyme activity assay | galactan | Geobacillus stearothermophilus | <a href=https://pubmed.ncbi.nlm.nih.gov/23216604/>23216604</a> Functional characterization of the galactan utilization system of Geobacillus stearothermophilus. FEBS J. 2013 Feb;280(3):950-64. doi: 10.1111/febs.12089. Epub 2013 Jan 7. |
2013 Feb | degradation | 7 | 2 | CBM61, GH53, CBM61, GH42 |
| PUL0284 | microarray, high-performance anion-exchange chromatography, liquid chromatography and mass spectrometry | galactan | Bifidobacterium breve | <a href=https://pubmed.ncbi.nlm.nih.gov/23199239/>23199239</a> Transcriptional and functional characterization of genetic elements involved in galacto-oligosaccharide utilization by Bifidobacterium breve UCC2003. Microb Biotechnol. 2013 Jan;6(1):67-79. doi: 10.1111/1751-7915.12011. Epub 2012 Dec 2. |
2013 Jan | degradation | 3 | 1 | GH2 |
| PUL0297 | gene trait matching exercise | galactan | Bifidobacterium longum | <a href=https://pubmed.ncbi.nlm.nih.gov/29310579/>29310579</a> Gene-trait matching across the Bifidobacterium longum pan-genome reveals considerable diversity in carbohydrate catabolism among human infant strains. BMC Genomics. 2018 Jan 8;19(1):33. doi: 10.1186/s12864-017-4388-9. |
2018 Jan 8 | degradation | 9 | 2 | GH42, GH53, CBM61 |
| PUL0298 | gene trait matching exercise | galactan | Bifidobacterium longum | <a href=https://pubmed.ncbi.nlm.nih.gov/29310579/>29310579</a> Gene-trait matching across the Bifidobacterium longum pan-genome reveals considerable diversity in carbohydrate catabolism among human infant strains. BMC Genomics. 2018 Jan 8;19(1):33. doi: 10.1186/s12864-017-4388-9. |
2018 Jan 8 | degradation | 10 | 1 | GH42 |
| PUL0304 | RT-qPCR, isothermal titration calorimetry (ITC), enzyme activity assay, gene deletion mutant and growth assay | galactan | Bacteroides thetaiotaomicron | <a href=https://pubmed.ncbi.nlm.nih.gov/29255254/>29255254</a> Dietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides. Nat Microbiol. 2018 Feb;3(2):210-219. doi: 10.1038/s41564-017-0079-1. Epub 2017 Dec 18. |
2018 Feb | degradation | 7 | 2 | GH2, GH53 |
| PUL0323 | fitness contribution assay, insertion sequencing | galactan | Bacillus subtilis subsp. subtilis | <a href=https://pubmed.ncbi.nlm.nih.gov/28617843/>28617843</a> Characterization of the regulation of a plant polysaccharide utilization operon and its role in biofilm formation in Bacillus subtilis. PLoS One. 2017 Jun 15;12(6):e0179761. doi: 10.1371/journal.pone.0179761. eCollection 2017. |
2017 | degradation | 6 | 2 | GH42, GH53 |
| PUL0393 | enzyme activity assay, analysis of reaction products | galactan | Microbulbifer thermotolerans | <a href=https://pubmed.ncbi.nlm.nih.gov/20686828/>20686828</a> Hyper-production and characterization of the iota-carrageenase useful for iota-carrageenan oligosaccharide production from a deep-sea bacterium, Microbulbifer thermotolerans JAMB-A94T, and insight into the unusual catalytic mechanism. Mar Biotechnol (NY). 2011 Jun;13(3):411-22. doi: 10.1007/s10126-010-9312-0. Epub 2010 Aug 5. |
2011 Jun | degradation | 5 | 2 | CBM6, CBM6, GH86, GH86, GH16_16, CBM6 |
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