| PULID | Characterization Method(s) | Substrate | Organism | Publication | Publish Date | Type | Num Genes | Num CAZymes | CazyFamily |
|---|---|---|---|---|---|---|---|---|---|
| PUL0027 | Northern Blot, gene deletion mutant and growth assay | alginate | Azotobacter vinelandii | <a href=https://pubmed.ncbi.nlm.nih.gov/10352233/>10352233</a> Transcriptional organization of the Azotobacter vinelandii algGXLVIFA genes: characterization of algF mutants. Gene. 1999 May 31;232(2):217-22. doi: 10.1016/s0378-1119(99)00119-5. |
1999 May 31 | biosynthesis | 5 | 1 | PL5_1 |
| PUL0051 | sequence homology analysis, NMR, size-exclusion chromatography (SEC), clone and expression, recombinant protein expression, enzyme kinetic analysis | alginate | Cellulophaga lytica | <a href=https://pubmed.ncbi.nlm.nih.gov/29795267/>29795267</a> Ancient acquisition of "alginate utilization loci" by human gut microbiota. Sci Rep. 2018 May 23;8(1):8075. doi: 10.1038/s41598-018-26104-1. |
2018 May 23 | degradation | 6 | 2 | PL17_2, PL17, PL6, PL6_1 |
| PUL0052 | sequence homology analysis, NMR, size-exclusion chromatography (SEC), clone and expression, recombinant protein expression, enzyme kinetic analysis | alginate | Maricaulis maris | <a href=https://pubmed.ncbi.nlm.nih.gov/29795267/>29795267</a> Ancient acquisition of "alginate utilization loci" by human gut microbiota. Sci Rep. 2018 May 23;8(1):8075. doi: 10.1038/s41598-018-26104-1. |
2018 May 23 | degradation | 10 | 2 | PL17_2, PL17, PL6, PL6_1 |
| PUL0053 | sequence homology analysis, NMR, size-exclusion chromatography (SEC), clone and expression, recombinant protein expression, enzyme kinetic analysis | alginate | Stenotrophomonas maltophilia | <a href=https://pubmed.ncbi.nlm.nih.gov/29795267/>29795267</a> Ancient acquisition of "alginate utilization loci" by human gut microbiota. Sci Rep. 2018 May 23;8(1):8075. doi: 10.1038/s41598-018-26104-1. |
2018 May 23 | degradation | 8 | 2 | PL17_2, PL17, PL6 |
| PUL0054 | sequence homology analysis, NMR, size-exclusion chromatography (SEC), clone and expression, recombinant protein expression, enzyme kinetic analysis | alginate | Alteromonas macleodii | <a href=https://pubmed.ncbi.nlm.nih.gov/29795267/>29795267</a> Ancient acquisition of "alginate utilization loci" by human gut microbiota. Sci Rep. 2018 May 23;8(1):8075. doi: 10.1038/s41598-018-26104-1. |
2018 May 23 | degradation | 9 | 2 | PL17_2, PL17, PL6, PL6_1 |
| PUL0055 | sequence homology analysis, NMR, size-exclusion chromatography (SEC), clone and expression, recombinant protein expression, enzyme kinetic analysis | alginate | Bacteroides sp. 1_1_30 | <a href=https://pubmed.ncbi.nlm.nih.gov/29795267/>29795267</a> Ancient acquisition of "alginate utilization loci" by human gut microbiota. Sci Rep. 2018 May 23;8(1):8075. doi: 10.1038/s41598-018-26104-1. |
2018 May 23 | degradation | 7 | 2 | PL17_2, PL17, PL6, PL6_1 |
| PUL0056 | sequence homology analysis, NMR, size-exclusion chromatography (SEC), clone and expression, recombinant protein expression, enzyme kinetic analysis | alginate | Bacteroides eggerthii | <a href=https://pubmed.ncbi.nlm.nih.gov/29795267/>29795267</a> Ancient acquisition of "alginate utilization loci" by human gut microbiota. Sci Rep. 2018 May 23;8(1):8075. doi: 10.1038/s41598-018-26104-1. |
2018 May 23 | degradation | 5 | 3 | CE20, PL17_2, PL17, PL6, PL6_1 |
| PUL0126 | growth assay, sequence homology analysis | alginate | Alteromonas sp. 76-1 | <a href=https://pubmed.ncbi.nlm.nih.gov/30936857/>30936857</a> Adaptations of Alteromonas sp. 76-1 to Polysaccharide Degradation: A CAZyme Plasmid for Ulvan Degradation and Two Alginolytic Systems. Front Microbiol. 2019 Mar 18;10:504. doi: 10.3389/fmicb.2019.00504. eCollection 2019. |
2019 | degradation | 8 | 2 | PL6, PL6_1, PL7_5 |
| PUL0127 | growth assay, sequence homology analysis | alginate | Alteromonas sp. 76-1 | <a href=https://pubmed.ncbi.nlm.nih.gov/30936857/>30936857</a> Adaptations of Alteromonas sp. 76-1 to Polysaccharide Degradation: A CAZyme Plasmid for Ulvan Degradation and Two Alginolytic Systems. Front Microbiol. 2019 Mar 18;10:504. doi: 10.3389/fmicb.2019.00504. eCollection 2019. |
2019 | degradation | 12 | 4 | CBM32, PL7_5, PL6_3, PL6, PL6_1, PL7_5 |
| PUL0150 | sequence homology analysis | alginate | Pseudoalteromonas carrageenovora | <a href=https://pubmed.ncbi.nlm.nih.gov/30524390/>30524390</a> Evolutionary Evidence of Algal Polysaccharide Degradation Acquisition by Pseudoalteromonas carrageenovora 9(T) to Adapt to Macroalgal Niches. Front Microbiol. 2018 Nov 22;9:2740. doi: 10.3389/fmicb.2018.02740. eCollection 2018. |
2018 | degradation | 14 | 3 | PL17_2, PL17, PL6_3, PL6, PL6_1 |
| PUL0151 | sequence homology analysis, Northern Blot, RT-qPCR, electrophoretic mobility shift assay, clone and expression, gene deletion mutant and growth assay | alginate | Zobellia galactanivorans | <a href=https://pubmed.ncbi.nlm.nih.gov/30524390/>30524390</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/32585009/>32585009</a> Evolutionary Evidence of Algal Polysaccharide Degradation Acquisition by Pseudoalteromonas carrageenovora 9(T) to Adapt to Macroalgal Niches. Regulation of alginate catabolism involves a GntR family repressor in the marine flavobacterium Zobellia galactanivorans DsijT. Front Microbiol. 2018 Nov 22;9:2740. doi: 10.3389/fmicb.2018.02740. eCollection 2018. Nucleic Acids Res. 2020 Aug 20;48(14):7786-7800. doi: 10.1093/nar/gkaa533. |
2018,2020 Aug 20 | degradation | 12 | 2 | PL17_2, PL17, PL7 |
| PUL0152 | sequence homology analysis | alginate | Pseudoalteromonas atlantica | <a href=https://pubmed.ncbi.nlm.nih.gov/30524390/>30524390</a> Evolutionary Evidence of Algal Polysaccharide Degradation Acquisition by Pseudoalteromonas carrageenovora 9(T) to Adapt to Macroalgal Niches. Front Microbiol. 2018 Nov 22;9:2740. doi: 10.3389/fmicb.2018.02740. eCollection 2018. |
2018 | degradation | 8 | 1 | PL6, PL6_1 |
| PUL0155 | enzyme activity assay | alginate | Agrobacterium fabrum | <a href=https://pubmed.ncbi.nlm.nih.gov/16545947/>16545947</a> A biosystem for alginate metabolism in Agrobacterium tumefaciens strain C58: molecular identification of Atu3025 as an exotype family PL-15 alginate lyase. Res Microbiol. 2006 Sep;157(7):642-9. doi: 10.1016/j.resmic.2006.02.006. Epub 2006 Mar 2. |
2006 Sep | degradation | 8 | 1 | PL15_1 |
| PUL0199 | enzyme activity assay, liquid chromatography and mass spectrometry | alginate | Saccharophagus degradans | <a href=https://pubmed.ncbi.nlm.nih.gov/26458373/>26458373</a> Putative Alginate Assimilation Process of the Marine Bacterium Saccharophagus degradans 2-40 Based on Quantitative Proteomic Analysis. Mar Biotechnol (NY). 2016 Feb;18(1):15-23. doi: 10.1007/s10126-015-9667-3. Epub 2015 Oct 12. |
2016 Feb | degradation | 17 | 6 | CBM16, CBM32, PL18, PL17_2, PL17, PL6, PL6, PL6_1, PL7_5 |
| PUL0216 | Western Blot, enzyme activity assay, RT-PCR, microarray | alginate | Sphingomonas sp. | <a href=https://pubmed.ncbi.nlm.nih.gov/24816607/>24816607</a> Alginate-dependent gene expression mechanism in Sphingomonas sp. strain A1. J Bacteriol. 2014 Jul;196(14):2691-700. doi: 10.1128/JB.01666-14. Epub 2014 May 9. |
2014 Jul | degradation | 10 | 3 | AA2, PL15_1, PL5, PL7 |
| PUL0235 | proteome fractionation, mass spectrometry, target decoy database analysis | alginate | Gramella forsetii | <a href=https://pubmed.ncbi.nlm.nih.gov/24522261/>24522261</a> Functional characterization of polysaccharide utilization loci in the marine Bacteroidetes 'Gramella forsetii' KT0803. ISME J. 2014 Jul;8(7):1492-502. doi: 10.1038/ismej.2014.4. Epub 2014 Feb 13. |
2014 Jul | degradation | 20 | 6 | PL17_2, PL17, PL6, PL6_1, PL7, PL7_5 |
| PUL0313 | microarray, Northern Blot, RT-qPCR, electrophoretic mobility shift assay, clone and expression, gene deletion mutant and growth assay | alginate | Zobellia galactanivorans | <a href=https://pubmed.ncbi.nlm.nih.gov/28983288/>28983288</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/32585009/>32585009</a> Gene Expression Analysis of Zobellia galactanivorans during the Degradation of Algal Polysaccharides Reveals both Substrate-Specific and Shared Transcriptome-Wide Responses. Regulation of alginate catabolism involves a GntR family repressor in the marine flavobacterium Zobellia galactanivorans DsijT. Front Microbiol. 2017 Sep 21;8:1808. doi: 10.3389/fmicb.2017.01808. eCollection 2017. Nucleic Acids Res. 2020 Aug 20;48(14):7786-7800. doi: 10.1093/nar/gkaa533. |
2017,2020 Aug 20 | degradation | 3 | 3 | PL6, PL6_1, PL7_5 |
| PUL0400 | RT-qPCR, RNA-seq | alginate | Alteromonas macleodii | <a href=https://pubmed.ncbi.nlm.nih.gov/25847866/>25847866</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/30116038/>30116038</a> Different utilization of alginate and other algal polysaccharides by marine Alteromonas macleodii ecotypes. Biphasic cellular adaptations and ecological implications of Alteromonas macleodii degrading a mixture of algal polysaccharides. Environ Microbiol. 2015 Oct;17(10):3857-68. doi: 10.1111/1462-2920.12862. Epub 2015 May 8. ISME J. 2019 Jan;13(1):92-103. doi: 10.1038/s41396-018-0252-4. Epub 2018 Aug 16. |
2015 Oct,2019 Jan | degradation | 14 | 5 | CBM32, PL7_5, PL17_2, PL17, PL6_3, PL6, PL6_1, PL7_5 |
| PUL0445 | recombinant protein expression, thin-layer chromatography, enzyme activity assay | alginate | Sphingomonas sp. | <a href=https://pubmed.ncbi.nlm.nih.gov/10913091/>10913091</a> Molecular identification of oligoalginate lyase of Sphingomonas sp. strain A1 as one of the enzymes required for complete depolymerization of alginate. J Bacteriol. 2000 Aug;182(16):4572-7. doi: 10.1128/JB.182.16.4572-4577.2000. |
2000 Aug | degradation | 8 | 2 | PL15_1, PL5, PL7 |
| PUL0572 | enzyme activity assay | alginate | Pseudomonas aeruginosa | <a href=https://pubmed.ncbi.nlm.nih.gov/8335634/>8335634</a> Characterization of the Pseudomonas aeruginosa alginate lyase gene (algL): cloning, sequencing, and expression in Escherichia coli. J Bacteriol. 1993 Aug;175(15):4780-9. doi: 10.1128/jb.175.15.4780-4789.1993. |
1993 Aug | biosynthesis | 12 | 2 | GT2, PL5_1 |
| PUL0631 | growth assay, sequence homology analysis | alginate | Pseudooceanicola algae Lw-13e | <a href=https://pubmed.ncbi.nlm.nih.gov/33310406/>33310406</a> Pseudooceanicola algae sp. nov., isolated from the marine macroalga Fucus spiralis, shows genomic and physiological adaptations for an algae-associated lifestyle. Syst Appl Microbiol. 2021 Jan;44(1):126166. doi: 10.1016/j.syapm.2020.126166. Epub 2020 Nov 27. |
2021 Jan | degradation | 8 | 1 | PL15_1 |
| PUL0654 | sequence homology analysis | alginate | Maribacter dokdonensis 62-1 | <a href=https://pubmed.ncbi.nlm.nih.gov/33912144/>33912144</a> CAZymes in Maribacter dokdonensis 62-1 From the Patagonian Shelf: Genomics and Physiology Compared to Related Flavobacteria and a Co-occurring Alteromonas Strain. Front Microbiol. 2021 Apr 12;12:628055. doi: 10.3389/fmicb.2021.628055. eCollection 2021. |
2021 | degradation | 38 | 7 | GH144, GH3, PL12, PL17_2, PL17, PL6, PL6_1 |
| PUL0655 | sequence homology analysis | alginate | Maribacter dokdonensis 62-1 | <a href=https://pubmed.ncbi.nlm.nih.gov/33912144/>33912144</a> CAZymes in Maribacter dokdonensis 62-1 From the Patagonian Shelf: Genomics and Physiology Compared to Related Flavobacteria and a Co-occurring Alteromonas Strain. Front Microbiol. 2021 Apr 12;12:628055. doi: 10.3389/fmicb.2021.628055. eCollection 2021. |
2021 | degradation | 10 | 2 | PL7 |
| PUL0691 | recombinant protein expression, qRT-PCR | alginate | Vibrio pelagius WXL662 | <a href=https://pubmed.ncbi.nlm.nih.gov/36409133/>36409133</a> Characterization of Multiple Alginate Lyases in a Highly Efficient Alginate-Degrading Vibrio Strain and Its Degradation Strategy. Appl Environ Microbiol. 2022 Dec 13;88(23):e0138922. doi: 10.1128/aem.01389-22. Epub 2022 Nov 21. |
2022 Dec 13 | degradation | 12 | 2 | CBM32, PL7_5, PL7, PL7 |
| PUL0692 | recombinant protein expression, qRT-PCR | alginate | Vibrio pelagius WXL662 | <a href=https://pubmed.ncbi.nlm.nih.gov/36409133/>36409133</a> Characterization of Multiple Alginate Lyases in a Highly Efficient Alginate-Degrading Vibrio Strain and Its Degradation Strategy. Appl Environ Microbiol. 2022 Dec 13;88(23):e0138922. doi: 10.1128/aem.01389-22. Epub 2022 Nov 21. |
2022 Dec 13 | degradation | 26 | 2 | PL17_2, PL17 |
| PUL0693 | recombinant protein expression, qRT-PCR | alginate | Vibrio pelagius WXL662 | <a href=https://pubmed.ncbi.nlm.nih.gov/36409133/>36409133</a> Characterization of Multiple Alginate Lyases in a Highly Efficient Alginate-Degrading Vibrio Strain and Its Degradation Strategy. Appl Environ Microbiol. 2022 Dec 13;88(23):e0138922. doi: 10.1128/aem.01389-22. Epub 2022 Nov 21. |
2022 Dec 13 | degradation | 17 | 2 | PL17_1, PL38 |
| PUL0715 | clone and expression, enzyme activity assay, ultra-performance liquid chromatography–mass spectrometry(UPLC-MS) | alginate | Wenyingzhuangia fucanilytica CZ1127 | <a href=https://pubmed.ncbi.nlm.nih.gov/37540808/>37540808</a> A repertoire of alginate lyases in the alginate polysaccharide utilization loci of marine bacterium Wenyingzhuangia fucanilytica: biochemical properties and action pattern. J Sci Food Agric. 2024 Jan 15;104(1):134-140. doi: 10.1002/jsfa.12898. Epub 2023 Aug 23. |
2024 Jan 15 | degradation | 12 | 4 | PL17_2, PL17, PL6, PL6_1, PL7 |
| PUL0718 | LC-ESI-MS, enzyme activity assay, recombinant protein expression, enzyme kinetic analysis | alginate | Bacteroides ovatus strain CP926 | <a href=https://pubmed.ncbi.nlm.nih.gov/37791757/>37791757</a> Three alginate lyases provide a new gut Bacteroides ovatus isolate with the ability to grow on alginate. Appl Environ Microbiol. 2023 Oct 31;89(10):e0118523. doi: 10.1128/aem.01185-23. Epub 2023 Oct 4. |
2023 Oct 31 | degradation | 12 | 3 | PL17_2, PL17, PL38, PL6, PL6_1 |
| PUL0736 | RNA-seq, RT-qPCR, enzyme activity assay, thin-layer chromatography, Western Blot, recombinant protein expression, DSS-induced mouse colitis model | alginate | Bacteroides clarus YIT 12056 | <a href=https://pubmed.ncbi.nlm.nih.gov/38563787/>38563787</a> Alginate oligosaccharide assimilation by gut microorganisms and the potential role in gut inflammation alleviation. Appl Environ Microbiol. 2024 May 21;90(5):e0004624. doi: 10.1128/aem.00046-24. Epub 2024 Apr 2. |
2024 May 21 | degradation | 10 | 3 | CE20, PL17_2, PL17, PL6, PL6_1 |
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