| PULID | Characterization Method(s) | Substrate | Organism | Publication | Publish Date | Type | Num Genes | Num CAZymes | CazyFamily |
|---|---|---|---|---|---|---|---|---|---|
| PUL0004 | enzyme activity assay, substrate binding assay | beta-glucan | uncultured bacterium | 26827771 A novel metagenome-derived gene cluster from termite hindgut: Encoding phosphotransferase system components and high glucose tolerant glucosidase. Enzyme Microb Technol. 2016 Mar;84:24-31. doi: 10.1016/j.enzmictec.2015.12.005. Epub 2015 Dec 15. |
2016 Mar | degradation | 2 | 1 | GH1 |
| PUL0024 | enzyme activity assay, qPCR, carbohydrate binding assay | fructan | uncultured bacterium | 31915220 Harvesting of Prebiotic Fructooligosaccharides by Nonbeneficial Human Gut Bacteria. mSphere. 2020 Jan 8;5(1):e00771-19. doi: 10.1128/mSphere.00771-19. |
2020 Jan 8 | degradation | 12 | 1 | GH32 |
| PUL0093 | fosmid library screen, lectin binding assay | host glycan | uncultured bacterium | 31275257 Investigating Host Microbiota Relationships Through Functional Metagenomics. Front Microbiol. 2019 Jun 7;10:1286. doi: 10.3389/fmicb.2019.01286. eCollection 2019. |
2019 | degradation | 24 | 7 | CE20, CE9, GH2, GH20, GH92 |
| PUL0094 | fosmid library screen, lectin binding assay | host glycan | uncultured bacterium | 31275257 Investigating Host Microbiota Relationships Through Functional Metagenomics. Front Microbiol. 2019 Jun 7;10:1286. doi: 10.3389/fmicb.2019.01286. eCollection 2019. |
2019 | degradation | 25 | 7 | CBM93, GH33, CE3, CE20, GH171, GH2, GH20, GH27 |
| PUL0095 | fosmid library screen, lectin binding assay | host glycan | uncultured bacterium | 31275257 Investigating Host Microbiota Relationships Through Functional Metagenomics. Front Microbiol. 2019 Jun 7;10:1286. doi: 10.3389/fmicb.2019.01286. eCollection 2019. |
2019 | degradation | 17 | 6 | CBM93, GH33, CE3, CE20, GH2, GH20, GH27 |
| PUL0096 | fosmid library screen, lectin binding assay | host glycan | uncultured bacterium | 31275257 Investigating Host Microbiota Relationships Through Functional Metagenomics. Front Microbiol. 2019 Jun 7;10:1286. doi: 10.3389/fmicb.2019.01286. eCollection 2019. |
2019 | degradation | 22 | 12 | CBM93, GH33, CE3, CE3, CE20, CE9, GH2, GH20, GH29, GH92, GH97 |
| PUL0102 | fosmid library screen, lectin binding assay | host glycan | uncultured bacterium | 31275257 Investigating Host Microbiota Relationships Through Functional Metagenomics. Front Microbiol. 2019 Jun 7;10:1286. doi: 10.3389/fmicb.2019.01286. eCollection 2019. |
2019 | degradation | 25 | 6 | CBM67, GH78, CBM93, GH33, CE20, CE3, GH20, GH29 |
| PUL0103 | fosmid library screen, lectin binding assay | host glycan | uncultured bacterium | 31275257 Investigating Host Microbiota Relationships Through Functional Metagenomics. Front Microbiol. 2019 Jun 7;10:1286. doi: 10.3389/fmicb.2019.01286. eCollection 2019. |
2019 | degradation | 31 | 2 | CBM67, GH78, CBM93, GH33 |
| PUL0104 | fosmid library screen, lectin binding assay | host glycan | uncultured bacterium | 31275257 Investigating Host Microbiota Relationships Through Functional Metagenomics. Front Microbiol. 2019 Jun 7;10:1286. doi: 10.3389/fmicb.2019.01286. eCollection 2019. |
2019 | degradation | 33 | 2 | CBM67, GH78, CBM93, GH33 |
| PUL0105 | fosmid library screen, lectin binding assay | host glycan | uncultured bacterium | 31275257 Investigating Host Microbiota Relationships Through Functional Metagenomics. Front Microbiol. 2019 Jun 7;10:1286. doi: 10.3389/fmicb.2019.01286. eCollection 2019. |
2019 | degradation | 36 | 5 | CBM67, GH78, CBM93, GH33, GH115, GH3, GH97 |
| PUL0106 | fosmid library screen, lectin binding assay | host glycan | uncultured bacterium | 31275257 Investigating Host Microbiota Relationships Through Functional Metagenomics. Front Microbiol. 2019 Jun 7;10:1286. doi: 10.3389/fmicb.2019.01286. eCollection 2019. |
2019 | degradation | 22 | 4 | GH2, GH20, CBM32 |
| PUL0107 | fosmid library screen, lectin binding assay | host glycan | uncultured bacterium | 31275257 Investigating Host Microbiota Relationships Through Functional Metagenomics. Front Microbiol. 2019 Jun 7;10:1286. doi: 10.3389/fmicb.2019.01286. eCollection 2019. |
2019 | degradation | 27 | 1 | GH1 |
| PUL0109 | fosmid library screen, lectin binding assay | host glycan | uncultured bacterium | 31275257 Investigating Host Microbiota Relationships Through Functional Metagenomics. Front Microbiol. 2019 Jun 7;10:1286. doi: 10.3389/fmicb.2019.01286. eCollection 2019. |
2019 | degradation | 28 | 1 | GH2 |
| PUL0110 | fosmid library screen, lectin binding assay | host glycan | uncultured bacterium | 31275257 Investigating Host Microbiota Relationships Through Functional Metagenomics. Front Microbiol. 2019 Jun 7;10:1286. doi: 10.3389/fmicb.2019.01286. eCollection 2019. |
2019 | degradation | 28 | 1 | GH2 |
| PUL0112 | fosmid library screen, lectin binding assay | host glycan | uncultured bacterium | 31275257 Investigating Host Microbiota Relationships Through Functional Metagenomics. Front Microbiol. 2019 Jun 7;10:1286. doi: 10.3389/fmicb.2019.01286. eCollection 2019. |
2019 | degradation | 25 | 4 | GH2, GH20, CBM32 |
| PUL0221 | fosmid library screen | cellulose | uncultured bacterium Contig1529 | 24223817 Metagenomic insights into the carbohydrate-active enzymes carried by the microorganisms adhering to solid digesta in the rumen of cows. PLoS One. 2013 Nov 5;8(11):e78507. doi: 10.1371/journal.pone.0078507. eCollection 2013. |
2013 | degradation | 10 | 4 | GH105, GH3, GH35, GH5_4 |
| PUL0222 | fosmid library screen | cellulose | uncultured bacterium Contig196 | 24223817 Metagenomic insights into the carbohydrate-active enzymes carried by the microorganisms adhering to solid digesta in the rumen of cows. PLoS One. 2013 Nov 5;8(11):e78507. doi: 10.1371/journal.pone.0078507. eCollection 2013. |
2013 | degradation | 7 | 3 | GH26, GH5_4, GH5_7 |
| PUL0332 | fosmid library screen, enzyme activity assay, thin-layer chromatography | beta-glucan | uncultured bacterium | 28091525 A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 19 | 8 | CE7, GH127, GH2, GH5_2, GH5_7, GH94, GH97 |
| PUL0333 | fosmid library screen, enzyme activity assay, thin-layer chromatography | beta-glucan | uncultured bacterium | 28091525 A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 22 | 4 | GH30, GH31_3, GH9 |
| PUL0334 | fosmid library screen, enzyme activity assay, thin-layer chromatography | beta-glucan | uncultured bacterium | 28091525 A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 23 | 7 | CE20, CE4, GH30, GH31_3, GH9 |
| PUL0335 | fosmid library screen, enzyme activity assay, thin-layer chromatography | xylan | uncultured bacterium | 28091525 A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 31 | 5 | GH13_46, GH158, GH16_3, GH3, GH97 |
| PUL0336 | fosmid library screen, enzyme activity assay, thin-layer chromatography | xylan | uncultured bacterium | 28091525 A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 25 | 4 | GH158, GH16_3, GH3, GT2 |
| PUL0337 | fosmid library screen, enzyme activity assay, thin-layer chromatography | xylan | uncultured bacterium | 28091525 A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 29 | 4 | GH158, GH16_3, GH3, GT2 |
| PUL0338 | fosmid library screen, enzyme activity assay, thin-layer chromatography | xylan | uncultured bacterium | 28091525 A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 34 | 5 | GH158, GH16_3, GH3, GH97, GT2 |
| PUL0339 | fosmid library screen, enzyme activity assay, thin-layer chromatography | xylan | uncultured bacterium | 28091525 A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 24 | 4 | GH16_3, GH20, GH3, GH97 |
| PUL0340 | fosmid library screen, enzyme activity assay, thin-layer chromatography | beta-glucan | uncultured bacterium | 28091525 A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 37 | 1 | GH5_2 |
| PUL0341 | fosmid library screen, enzyme activity assay, thin-layer chromatography | beta-glucan | uncultured bacterium | 28091525 A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 43 | 3 | GH32, GH5_2, GH91 |
| PUL0343 | gene deletion mutant and growth assay, enzyme activity assay, Western Blot, isothermal titration calorimetry (ITC) | beta-glucan | uncultured bacterium | 28091525 A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 39 | 1 | GH5_2 |
| PUL0346 | gene deletion mutant and growth assay | xylan | uncultured bacterium | 24066026 Functional metagenomics reveals novel pathways of prebiotic breakdown by human gut bacteria. Functional characterization of a gene locus from an uncultured gut Bacteroides conferring xylo-oligosaccharides utilization to Escherichia coli. PLoS One. 2013 Sep 16;8(9):e72766. doi: 10.1371/journal.pone.0072766. eCollection 2013. Mol Microbiol. 2016 Nov;102(4):579-592. doi: 10.1111/mmi.13480. Epub 2016 Sep 14. |
2013,2016 Nov | degradation | 13 | 5 | GH10, GH16_3, GH43_1, GH43_12, CBM91, GH43_29 |
| PUL0413 | enzyme activity assay, reducing-sugar assay | cellobiose | uncultured bacterium contig00059 | 30116044 Functional metagenomics reveals abundant polysaccharide-degrading gene clusters and cellobiose utilization pathways within gut microbiota of a wood-feeding higher termite. ISME J. 2019 Jan;13(1):104-117. doi: 10.1038/s41396-018-0255-1. Epub 2018 Aug 16. |
2019 Jan | degradation | 31 | 2 | GH1, GH44 |
| PUL0414 | enzyme activity assay, thin-layer chromatography | xylan | uncultured bacterium 35A20 | 30116044 Functional metagenomics reveals abundant polysaccharide-degrading gene clusters and cellobiose utilization pathways within gut microbiota of a wood-feeding higher termite. ISME J. 2019 Jan;13(1):104-117. doi: 10.1038/s41396-018-0255-1. Epub 2018 Aug 16. |
2019 Jan | degradation | 25 | 4 | GH1, GH10 |
| PUL0749 | affinity gel electrophoresis | beta-glucan | uncultured bacterium | 39012103 Biochemical characterization of a SusD-like protein involved in beta-1,3-glucan utilization by an uncultured cow rumen Bacteroides. mSphere. 2024 Aug 28;9(8):e0027824. doi: 10.1128/msphere.00278-24. Epub 2024 Jul 16. |
2024 Aug 28 | degradation | 5 | 2 | GH16_3, GH3 |
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