dbCAN-PUL

Browse dbCAN-PUL Entries

PULID Characterization Method(s) Substrate Organism Publication Publish Date Type Num Genes Num CAZymes CazyFamily
PUL0018 rapid plate method growth assay, gene deletion mutant and growth assay, RT-PCR, enzyme activity assay glycosaminoglycan Streptococcus pneumoniae 22311922
Streptococcus pneumoniae can utilize multiple sources of hyaluronic acid for growth. Infect Immun. 2012 Apr;80(4):1390-8. doi: 10.1128/IAI.05756-11. Epub 2012 Feb 6.
2012 Apr degradation 13 3 CBM70, PL8_1, GH88, PL12_1
PUL0037 enzyme activity assay raffinose Streptococcus pneumoniae 31591266
Molecular analysis of an enigmatic Streptococcus pneumoniae virulence factor: The raffinose-family oligosaccharide utilization system. J Biol Chem. 2019 Nov 15;294(46):17197-17208. doi: 10.1074/jbc.RA119.010280. Epub 2019 Oct 7.
2019 Nov 15 degradation 8 2 GH13_18, GH36
PUL0186 gene deletion mutant and growth assay cellobiose Streptococcus pneumoniae 17028271
The two-component regulatory system TCS08 is involved in cellobiose metabolism of Streptococcus pneumoniae R6. J Bacteriol. 2007 Feb;189(4):1342-50. doi: 10.1128/JB.01170-06. Epub 2006 Oct 6.
2007 Feb degradation 7 1 GH1
PUL0194 enzyme activity assay, gene deletion mutant and growth assay host glycan Streptococcus pneumoniae 28056108
Molecular Characterization of N-glycan Degradation and Transport in Streptococcus pneumoniae and Its Contribution to Virulence. PLoS Pathog. 2017 Jan 5;13(1):e1006090. doi: 10.1371/journal.ppat.1006090. eCollection 2017 Jan.
2017 Jan degradation 6 5 GH125, GH20, GH29, GH38, GH92
PUL0245 enzyme activity assay, gene deletion mutant and growth assay, Western Blot fucose Streptococcus pneumoniae 24333485
Structural and functional analysis of fucose-processing enzymes from Streptococcus pneumoniae. J Mol Biol. 2014 Apr 3;426(7):1469-82. doi: 10.1016/j.jmb.2013.12.006. Epub 2013 Dec 12.
2014 Apr 3 degradation 11 2 GH95, GH98, CBM47, CBM47, CBM47
PUL0246 enzyme activity assay, gene deletion mutant and growth assay, Western Blot fucose Streptococcus pneumoniae 24333485
Structural and functional analysis of fucose-processing enzymes from Streptococcus pneumoniae. J Mol Biol. 2014 Apr 3;426(7):1469-82. doi: 10.1016/j.jmb.2013.12.006. Epub 2013 Dec 12.
2014 Apr 3 degradation 11 4 CBM51, CBM51, GH98, GH29, GH36
PUL0277 gene deletion mutant and growth assay, qRT-PCR fructan Streptococcus pneumoniae 23264576
The ABC transporter encoded at the pneumococcal fructooligosaccharide utilization locus determines the ability to utilize long- and short-chain fructooligosaccharides. J Bacteriol. 2013 Mar;195(5):1031-41. doi: 10.1128/JB.01560-12. Epub 2012 Dec 21.
2013 Mar degradation 5 1 GH32
PUL0278 gene deletion mutant and growth assay, qRT-PCR fructan Streptococcus pneumoniae 23264576
The ABC transporter encoded at the pneumococcal fructooligosaccharide utilization locus determines the ability to utilize long- and short-chain fructooligosaccharides. J Bacteriol. 2013 Mar;195(5):1031-41. doi: 10.1128/JB.01560-12. Epub 2012 Dec 21.
2013 Mar degradation 6 1 GH32
PUL0279 gene deletion mutant and growth assay, qRT-PCR fructan Streptococcus pneumoniae 23264576
The ABC transporter encoded at the pneumococcal fructooligosaccharide utilization locus determines the ability to utilize long- and short-chain fructooligosaccharides. J Bacteriol. 2013 Mar;195(5):1031-41. doi: 10.1128/JB.01560-12. Epub 2012 Dec 21.
2013 Mar degradation 6 1 GH32
PUL0486 RT-PCR, sugar utilization assay raffinose Streptococcus pneumoniae 10613841
Regulation of the alpha-galactosidase activity in Streptococcus pneumoniae: characterization of the raffinose utilization system. Genome Res. 1999 Dec;9(12):1189-97. doi: 10.1101/gr.9.12.1189.
1999 Dec degradation 8 2 GH13_18, GH36
PUL0519 gene deletion mutant and growth assay starch Streptococcus pneumoniae 8244973
Characterization of the Streptococcus pneumoniae maltosaccharide regulator MalR, a member of the LacI-GalR family of repressors displaying distinctive genetic features. J Biol Chem. 1993 Dec 5;268(34):25402-8.
1993 Dec 5 degradation 7 2 GH77, GT35
PUL0585 microarray, gene deletion mutant and growth assay, beta-galactosidase assays cellobiose Streptococcus pneumoniae 21778207
CelR-mediated activation of the cellobiose-utilization gene cluster in Streptococcus pneumoniae. Microbiology (Reading). 2011 Oct;157(Pt 10):2854-2861. doi: 10.1099/mic.0.051359-0. Epub 2011 Jul 21.
2011 Oct degradation 7 1 GH1