Browse dbCAN-PUL Entries

PULID Characterization Method(s) Substrate Organism Publication Publish Date Type Num Genes Num CAZymes CazyFamily
PUL0044 qRT-PCR, enzyme activity assay arabinoxylan Bacteroides ovatus <a href=https://pubmed.ncbi.nlm.nih.gov/26112186/>26112186</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/32266006/>32266006</a>
Glycan complexity dictates microbial resource allocation in the large intestine. Multimodular fused acetyl-feruloyl esterases from soil and gut Bacteroidetes improve xylanase depolymerization of recalcitrant biomass. Nat Commun. 2015 Jun 26;6:7481. doi: 10.1038/ncomms8481. Biotechnol Biofuels. 2020 Mar 31;13:60. doi: 10.1186/s13068-020-01698-9. eCollection 2020.
2015 Jun 26,2020 degradation 34 17 CE20, CE20, CE6, CE1, GH10, GH115, GH3, GH30, GH30_8, GH31_4, GH43_10, CBM91, GH43_12, CBM91, GH43_29, CBM6, GH95, GH97, GH98, CBM35
PUL0045 qRT-PCR arabinoxylan Bacteroides ovatus <a href=https://pubmed.ncbi.nlm.nih.gov/26112186/>26112186</a>
Glycan complexity dictates microbial resource allocation in the large intestine. Nat Commun. 2015 Jun 26;6:7481. doi: 10.1038/ncomms8481.
2015 Jun 26 degradation 11 5 CBM4, GH10, CE20, CE20, GH10, GH43_1, GH67
PUL0063 bicinchoninic acid (BCA) reducing-sugar assay, enzyme product analysis, affinity gel electrophoresis, isothermal titration calorimetry (ITC) xyloglucan Bacteroides ovatus <a href=https://pubmed.ncbi.nlm.nih.gov/31420336/>31420336</a>
Adaptation of Syntenic Xyloglucan Utilization Loci of Human Gut Bacteroidetes to Polysaccharide Side Chain Diversity. Appl Environ Microbiol. 2019 Oct 1;85(20):e01491-19. doi: 10.1128/AEM.01491-19. Print 2019 Oct 15.
2019 Oct 15 degradation 16 8 GH2, GH3, GH31_4, GH43_12, CBM91, GH5_4, GH9
PUL0167 mass spectrometry, sequence homology analysis beta-mannan Bacteroides ovatus <a href=https://pubmed.ncbi.nlm.nih.gov/30246424/>30246424</a>
Alpha- and beta-mannan utilization by marine Bacteroidetes. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16.
2018 Nov degradation 12 4 GH130_1, GH26, GH36
PUL0178 enzyme activity assay, enzyme specificity assay, substrate specificity assay galactomannan Bacteroides ovatus <a href=https://pubmed.ncbi.nlm.nih.gov/27288925/>27288925</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/22205877/>22205877</a>
A beta-mannan utilization locus in Bacteroides ovatus involves a GH36 alpha-galactosidase active on galactomannans. Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. FEBS Lett. 2016 Jul;590(14):2106-18. doi: 10.1002/1873-3468.12250. Epub 2016 Jun 28. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2016 Jul,2011 Dec degradation 14 4 GH130_1, GH26, GH36
PUL0179 enzyme activity assay, enzyme specificity assay, substrate specificity assay galactomannan Bacteroides ovatus <a href=https://pubmed.ncbi.nlm.nih.gov/27288925/>27288925</a>
A beta-mannan utilization locus in Bacteroides ovatus involves a GH36 alpha-galactosidase active on galactomannans. FEBS Lett. 2016 Jul;590(14):2106-18. doi: 10.1002/1873-3468.12250. Epub 2016 Jun 28.
2016 Jul degradation 15 4 CE7, GH130_1, GH26
PUL0306 RT-qPCR, isothermal titration calorimetry (ITC), enzyme activity assay, gene deletion mutant and growth assay pectin Bacteroides ovatus <a href=https://pubmed.ncbi.nlm.nih.gov/29255254/>29255254</a>
Dietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides. Nat Microbiol. 2018 Feb;3(2):210-219. doi: 10.1038/s41564-017-0079-1. Epub 2017 Dec 18.
2018 Feb degradation 7 3 GH147, GH2, GH53
PUL0326 gene deletion mutant and growth assay, enzyme activity assay, thin-layer chromatography beta-glucan Bacteroides ovatus <a href=https://pubmed.ncbi.nlm.nih.gov/28461332/>28461332</a>
A Bacteroidetes locus dedicated to fungal 1,6-beta-glucan degradation: Unique substrate conformation drives specificity of the key endo-1,6-beta-glucanase. J Biol Chem. 2017 Jun 23;292(25):10639-10650. doi: 10.1074/jbc.M117.787606. Epub 2017 May 1.
2017 Jun 23 degradation 13 1 GH73
PUL0415 microarray xylan Bacteroides ovatus <a href=https://pubmed.ncbi.nlm.nih.gov/22205877/>22205877</a>
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2011 Dec degradation 4 2 GH20, GH20, CBM32
PUL0416 microarray pectin Bacteroides ovatus <a href=https://pubmed.ncbi.nlm.nih.gov/22205877/>22205877</a>
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2011 Dec degradation 12 1 CE20
PUL0417 microarray pectin Bacteroides ovatus <a href=https://pubmed.ncbi.nlm.nih.gov/22205877/>22205877</a>
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2011 Dec degradation 13 1 CE20
PUL0418 microarray pectin Bacteroides ovatus <a href=https://pubmed.ncbi.nlm.nih.gov/22205877/>22205877</a>
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2011 Dec degradation 9 2 CE1
PUL0419 microarray pectin Bacteroides ovatus <a href=https://pubmed.ncbi.nlm.nih.gov/22205877/>22205877</a>
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2011 Dec degradation 11 7 CBM67, GH78, GH130_1, GH140, GH2, GH5_2, GH5_5
PUL0420 microarray pectin Bacteroides ovatus <a href=https://pubmed.ncbi.nlm.nih.gov/22205877/>22205877</a>
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2011 Dec degradation 4 2 GH144, GH3
PUL0421 microarray starch Bacteroides ovatus <a href=https://pubmed.ncbi.nlm.nih.gov/22205877/>22205877</a>
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2011 Dec degradation 7 3 GH13_10, GH13_46, GH97
PUL0422 microarray host glycan Bacteroides ovatus <a href=https://pubmed.ncbi.nlm.nih.gov/22205877/>22205877</a>
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2011 Dec degradation 4 1 GH18
PUL0424 microarray host glycan Bacteroides ovatus <a href=https://pubmed.ncbi.nlm.nih.gov/22205877/>22205877</a>
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2011 Dec degradation 18 5 GH130_2, GH163, GH18, GH92
PUL0425 microarray fructan Bacteroides ovatus <a href=https://pubmed.ncbi.nlm.nih.gov/22205877/>22205877</a>
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2011 Dec degradation 11 4 GH32, GH91
PUL0426 microarray mucin Bacteroides ovatus <a href=https://pubmed.ncbi.nlm.nih.gov/22205877/>22205877</a>
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2011 Dec degradation 13 4 GH18, GH92
PUL0427 microarray pectin Bacteroides ovatus <a href=https://pubmed.ncbi.nlm.nih.gov/22205877/>22205877</a>
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2011 Dec degradation 6 2 GH147, GH53
PUL0526 microarray, qPCR beta-glucan Bacteroides ovatus <a href=https://pubmed.ncbi.nlm.nih.gov/22205877/>22205877</a>
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2011 Dec degradation 6 2 GH3, GH30_3
PUL0527 microarray, qPCR xyloglucan Bacteroides ovatus <a href=https://pubmed.ncbi.nlm.nih.gov/22205877/>22205877</a>
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2011 Dec degradation 16 8 GH2, GH3, GH31_4, GH43_12, CBM91, GH5_4, GH9
PUL0528 microarray, qPCR beta-glucan Bacteroides ovatus <a href=https://pubmed.ncbi.nlm.nih.gov/22205877/>22205877</a>
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2011 Dec degradation 7 3 GH16_3, GH3
PUL0529 microarray, qPCR, RNA-seq, reducing-sugar assay, growth assay pectin Bacteroides ovatus <a href=https://pubmed.ncbi.nlm.nih.gov/22205877/>22205877</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/39892338/>39892338</a>
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. In vitro fermentation of a purified fraction of polysaccharides from the root of Brassica rapa L. by human gut microbiota and its interaction with Bacteroides ovatus. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. Food Chem. 2025 May 1;473:143109. doi: 10.1016/j.foodchem.2025.143109. Epub 2025 Jan 27.
2011 Dec,2025 May 1 degradation 27 13 CE12, CE8, CE8, GH105, GH28, GH3, GH43_10, CBM91, PL1_2
PUL0565 microarray, qPCR galactomannan Bacteroides ovatus <a href=https://pubmed.ncbi.nlm.nih.gov/22205877/>22205877</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/27872187/>27872187</a>
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. Galactomannan Catabolism Conferred by a Polysaccharide Utilization Locus of Bacteroides ovatus: ENZYME SYNERGY AND CRYSTAL STRUCTURE OF A beta-MANNANASE. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. J Biol Chem. 2017 Jan 6;292(1):229-243. doi: 10.1074/jbc.M116.746438. Epub 2016 Nov 21.
2011 Dec,2017 Jan 6 degradation 10 4 GH130_1, GH26, GH36
PUL0566 microarray, qPCR pectin Bacteroides ovatus <a href=https://pubmed.ncbi.nlm.nih.gov/22205877/>22205877</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/29255254/>29255254</a>
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. Dietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. Nat Microbiol. 2018 Feb;3(2):210-219. doi: 10.1038/s41564-017-0079-1. Epub 2017 Dec 18.
2011 Dec,2018 Feb degradation 7 3 GH147, GH2, GH53
PUL0674 microarray, enzyme activity assay, high-performance anion-exchange chromatography, mass spectrometry, RNA-seq, affinity gel electrophoresis, carbohydrate binding assay, microscale thermophoresis beta-glucan Bacteroides ovatus ATCC 8483 <a href=https://pubmed.ncbi.nlm.nih.gov/34817219/>34817219</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/29020628/>29020628</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/22205877/>22205877</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/32801182/>32801182</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/31062073/>31062073</a>
Mapping Molecular Recognition of beta1,3-1,4-Glucans by a Surface Glycan-Binding Protein from the Human Gut Symbiont Bacteroides ovatus. Molecular Mechanism by which Prominent Human Gut Bacteroidetes Utilize Mixed-Linkage Beta-Glucans, Major Health-Promoting Cereal Polysaccharides. Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. Sharing a beta-Glucan Meal: Transcriptomic Eavesdropping on a Bacteroides ovatus-Subdoligranulum variabile-Hungatella hathewayi Consortium. Surface glycan-binding proteins are essential for cereal beta-glucan utilization by the human gut symbiont Bacteroides ovatus. Microbiol Spectr. 2021 Dec 22;9(3):e0182621. doi: 10.1128/Spectrum.01826-21. Epub 2021 Nov 24. Cell Rep. 2017 Oct 10;21(2):417-430. doi: 10.1016/j.celrep.2017.09.049. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. Appl Environ Microbiol. 2020 Oct 1;86(20):e01651-20. doi: 10.1128/AEM.01651-20. Print 2020 Oct 1. Cell Mol Life Sci. 2019 Nov;76(21):4319-4340. doi: 10.1007/s00018-019-03115-3. Epub 2019 May 6.
2021 Dec 22,2017 Oct 10,2011 Dec,2020 Oct 1,2019 Nov degradation 8 3 GH16_3, GH3
PUL0714 clone and expression, crystallization, isothermal titration calorimetry (ITC), thin-layer chromatography, Western Blot, gene mutant starch Bacteroides ovatus strain ATCC 8483  <a href=https://pubmed.ncbi.nlm.nih.gov/37500984/>37500984</a>
BoGH13A(Sus) from Bacteroides ovatus represents a novel alpha-amylase used for Bacteroides starch breakdown in the human gut. Cell Mol Life Sci. 2023 Jul 28;80(8):232. doi: 10.1007/s00018-023-04812-w.
2023 Jul 28 degradation 8 3 GH13_10, GH13_46, GH97
PUL0718 LC-ESI-MS, enzyme activity assay, recombinant protein expression, enzyme kinetic analysis alginate Bacteroides ovatus strain CP926 <a href=https://pubmed.ncbi.nlm.nih.gov/37791757/>37791757</a>
Three alginate lyases provide a new gut Bacteroides ovatus isolate with the ability to grow on alginate. Appl Environ Microbiol. 2023 Oct 31;89(10):e0118523. doi: 10.1128/aem.01185-23. Epub 2023 Oct 4.
2023 Oct 31 degradation 12 3 PL17_2, PL17, PL38, PL6, PL6_1
PUL0745 high performance gel permeation chromatography, gas chromatography, RNA-seq, differential gene expression pectic polysaccharide Bacteroides ovatus strain ATCC 8483 <a href=https://pubmed.ncbi.nlm.nih.gov/38890895/>38890895</a>
The Utilization by Bacteroides spp. of a Purified Polysaccharide from Fuzhuan Brick Tea. Foods. 2024 May 26;13(11):1666. doi: 10.3390/foods13111666.
2024 May 26 degradation 12 4 GH146, GH28, GH30_2, PL12
PUL0746 high performance gel permeation chromatography, gas chromatography, RNA-seq, differential gene expression pectic polysaccharide Bacteroides ovatus strain ATCC 8483 <a href=https://pubmed.ncbi.nlm.nih.gov/38890895/>38890895</a>
The Utilization by Bacteroides spp. of a Purified Polysaccharide from Fuzhuan Brick Tea. Foods. 2024 May 26;13(11):1666. doi: 10.3390/foods13111666.
2024 May 26 degradation 7 4 CBM93, GH33, GH2, GH20, GH20, CBM32
PUL0747 high performance gel permeation chromatography, gas chromatography, RNA-seq, differential gene expression pectic polysaccharide Bacteroides ovatus strain ATCC 8483 <a href=https://pubmed.ncbi.nlm.nih.gov/38890895/>38890895</a>
The Utilization by Bacteroides spp. of a Purified Polysaccharide from Fuzhuan Brick Tea. Foods. 2024 May 26;13(11):1666. doi: 10.3390/foods13111666.
2024 May 26 degradation 9 3 GH2, GH20, GH29, CBM32
PUL0786 RNA-seq, reducing-sugar assay, growth assay, high performance gel permeation chromatography, gas chromatography, RNA-seq, differential gene expression pectic polysaccharide Bacteroides ovatus strain ATCC 8483 <a href=https://pubmed.ncbi.nlm.nih.gov/38890895/>38890895</a>, <a href=https://pubmed.ncbi.nlm.nih.gov/39892338/>39892338</a>
The Utilization by Bacteroides spp. of a Purified Polysaccharide from Fuzhuan Brick Tea. In vitro fermentation of a purified fraction of polysaccharides from the root of Brassica rapa L. by human gut microbiota and its interaction with Bacteroides ovatus. Foods. 2024 May 26;13(11):1666. doi: 10.3390/foods13111666. Food Chem. 2025 May 1;473:143109. doi: 10.1016/j.foodchem.2025.143109. Epub 2025 Jan 27.
2024 May 26,2025 May 1 degradation 26 14 CBM67, GH78, CBM67, GH78, GH33, CE19, GH105, GH130_2, GH140, GH143, GH142, GH163, GH18, GH28, GH43_18, GH92, GH95, PL1_2
PUL0787 RNA-seq, reducing-sugar assay, growth assay pectic polysaccharide Bacteroides ovatus strain ATCC 8483 <a href=https://pubmed.ncbi.nlm.nih.gov/39892338/>39892338</a>
In vitro fermentation of a purified fraction of polysaccharides from the root of Brassica rapa L. by human gut microbiota and its interaction with Bacteroides ovatus. Food Chem. 2025 May 1;473:143109. doi: 10.1016/j.foodchem.2025.143109. Epub 2025 Jan 27.
2025 May 1 degradation 31 16 CE12, CE12, CE12, CE4, GH105, GH106, GH2, GH28, GH42, GH43_18, GH43_34, PL11_1, PL26
PUL0788 RNA-seq, reducing-sugar assay, growth assay pectic polysaccharide Bacteroides ovatus strain ATCC 8483 <a href=https://pubmed.ncbi.nlm.nih.gov/39892338/>39892338</a>
In vitro fermentation of a purified fraction of polysaccharides from the root of Brassica rapa L. by human gut microbiota and its interaction with Bacteroides ovatus. Food Chem. 2025 May 1;473:143109. doi: 10.1016/j.foodchem.2025.143109. Epub 2025 Jan 27.
2025 May 1 degradation 26 8 CE20, GH105, GH2, GH28, PL11, PL1_2, PL9_1
PUL0789 RNA-seq, reducing-sugar assay, growth assay pectic polysaccharide Bacteroides ovatus strain ATCC 8483 <a href=https://pubmed.ncbi.nlm.nih.gov/39892338/>39892338</a>
In vitro fermentation of a purified fraction of polysaccharides from the root of Brassica rapa L. by human gut microbiota and its interaction with Bacteroides ovatus. Food Chem. 2025 May 1;473:143109. doi: 10.1016/j.foodchem.2025.143109. Epub 2025 Jan 27.
2025 May 1 degradation 9 2 CBM6, GH28
PUL0790 RNA-seq, reducing-sugar assay, growth assay pectic polysaccharide Bacteroides ovatus strain ATCC 8483 <a href=https://pubmed.ncbi.nlm.nih.gov/39892338/>39892338</a>
In vitro fermentation of a purified fraction of polysaccharides from the root of Brassica rapa L. by human gut microbiota and its interaction with Bacteroides ovatus. Food Chem. 2025 May 1;473:143109. doi: 10.1016/j.foodchem.2025.143109. Epub 2025 Jan 27.
2025 May 1 degradation 3 3 GH127, GH141, GH78
PUL0791 RNA-seq, reducing-sugar assay, growth assay pectic polysaccharide Bacteroides ovatus strain ATCC 8483 <a href=https://pubmed.ncbi.nlm.nih.gov/39892338/>39892338</a>
In vitro fermentation of a purified fraction of polysaccharides from the root of Brassica rapa L. by human gut microbiota and its interaction with Bacteroides ovatus. Food Chem. 2025 May 1;473:143109. doi: 10.1016/j.foodchem.2025.143109. Epub 2025 Jan 27.
2025 May 1 degradation 6 5 CE20, GH106, GH139, GH2, PL1_2