dbCAN-PUL

PUL ID	PUL0790
PubMed	39892338, Food Chem. 2025 May 1;473:143109. doi: 10.1016/j.foodchem.2025.143109. Epub 2025 Jan 27.
Characterization method	RNA-seq,reducing-sugar assay,growth assay
Genomic accession number	NZ_CP012938.1
Nucelotide position range	781108-787422
Substrate	pectic polysaccharide
Loci	Bovatus_RS03110-Bovatus_RS03120
Species	Bacteroides ovatus strain ATCC 8483/28116
Degradation or Biosynthesis	degradation

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PUL ID	PUL0790
PubMed	39892338, Food Chem. 2025 May 1;473:143109. doi: 10.1016/j.foodchem.2025.143109. Epub 2025 Jan 27.
Title	In vitro fermentation of a purified fraction of polysaccharides from the root of Brassica rapa L. by human gut microbiota and its interaction with Bacteroides ovatus.
Author	Yi W, Shi J, Zhou W, Wei J, Sun Y, Zeng X, Wang W
Abstract	In this study, the physicochemical properties, in vitro fermentation, and interaction with Bacteroides ovatus of a purified fraction of polysaccharides from the root of Brassica rapa (BRP2-2) were investigated. It was demonstrated with an in vitro anaerobic fermentation model that BRP2-2 significantly increased (p < 0.05) the relative abundance of Bacteroides spp. After 24 h incubation of BRP2-2 with Bacteroides ovatus alone, 10.24 +/- 0.69, 8.76 +/- 0.48 and 3.37 +/- 0.26 mM of acetate, propionate and isovalerate were produced, respectively. Moreover, transcriptome analysis of B. ovatus showed that 143 genes were up-regulated by BRP2-2, including five discrete polysaccharide utilization loci and two carbohydrate-active enzyme clusters. Based on the annotation of carbohydrate enzyme function, we speculate that BRP2-2 is a pectic polysaccharide containing homogalacturonan, rhamnogalacturonan I and rhamnogalacturonan II domains. These results suggested that BRP2-2 was degraded by B. ovatus, which produced metabolites with beneficial effects on host health.