Home Repository Download Help About Statistics Taxonomy Blastx
Links
PULDB
CAZy
dbCAN2
dbCAN-seq
run_dbcan
CAZypedia
PlantCAZyme
Cite: NAR/gkaa742 2020

Because CGCFinder predicted no CGC for this PUL, the gene cluster depicted below contains dbCAN2 and CGC signature predictions for all genes in the PUL, instead of a predicted CGC.


PUL General Information

Literature Information

PUL ID

PUL0782

PubMed

39738071, Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w.

Characterization method

RNA-seq,differential gene expression,HPAEC-PAD,SDS-PAGE,para-hydroxybenzoic acid (PAHBAH) assay,reducing-sugar assay,Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE),enzyme activity assay

Genomic accession number

NZ_CAWLDM010000001.1

Nucelotide position range

5325807-5350204

Substrate

fucoidan

Loci

None

Species

Neorhodopirellula lusitana/445327

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- ACI5KW_RS19310 - 0 - 846 (-) NZ_CAWLDM010000001.1:5325807-5326653 -
tnpC ACI5KW_RS19315 WP_404305570.1 913 - 2308 (+) NZ_CAWLDM010000001.1:5326720-5328115 -
- ACI5KW_RS19320 - 2313 - 2880 (-) NZ_CAWLDM010000001.1:5328120-5328687 -
- ACI5KW_RS19325 WP_404309440.1 3007 - 4780 (-) NZ_CAWLDM010000001.1:5328814-5330587 -
- ACI5KW_RS19330 WP_404309441.1 4871 - 6365 (-) NZ_CAWLDM010000001.1:5330678-5332172 -
- ACI5KW_RS19335 WP_404309442.1 6421 - 7345 (-) NZ_CAWLDM010000001.1:5332228-5333152 -
- ACI5KW_RS19340 WP_404309443.1 7298 - 8708 (-) NZ_CAWLDM010000001.1:5333105-5334515 -
- ACI5KW_RS19345 WP_404309444.1 8704 - 10345 (-) NZ_CAWLDM010000001.1:5334511-5336152 -
- ACI5KW_RS19350 WP_404311122.1 10344 - 11907 (-) NZ_CAWLDM010000001.1:5336151-5337714 3.2.1.51
- ACI5KW_RS19355 WP_404309445.1 12004 - 14899 (-) NZ_CAWLDM010000001.1:5337811-5340706 -
- ACI5KW_RS19360 WP_404309446.1 14999 - 15866 (-) NZ_CAWLDM010000001.1:5340806-5341673 -
- ACI5KW_RS19365 WP_404309447.1 15982 - 17338 (-) NZ_CAWLDM010000001.1:5341789-5343145 -
- ACI5KW_RS19370 WP_404309448.1 17391 - 18120 (-) NZ_CAWLDM010000001.1:5343198-5343927 -
- ACI5KW_RS19375 WP_404309449.1 18354 - 20769 (-) NZ_CAWLDM010000001.1:5344161-5346576 -
- ACI5KW_RS19380 WP_404309450.1 20783 - 22100 (-) NZ_CAWLDM010000001.1:5346590-5347907 -
- ACI5KW_RS19385 WP_404309451.1 22104 - 23862 (-) NZ_CAWLDM010000001.1:5347911-5349669 -
- ACI5KW_RS19390 WP_404309452.1 23858 - 24311 (-) NZ_CAWLDM010000001.1:5349665-5350118 -

Cluster number

0

Gene name

Gene position

Gene type

Found by CGCFinder?

- 914 - 2308 (+) CDS No
- 3008 - 4780 (-) CDS No
- 4872 - 6365 (-) CDS No
- 6422 - 7345 (-) CDS No
- 7299 - 8708 (-) CDS No
- 8705 - 10345 (-) CDS No
- 10345 - 11907 (-) CAZyme: GH29 No
- 12005 - 14899 (-) CAZyme: GH117 No
- 15000 - 15866 (-) CDS No
- 15983 - 17338 (-) CAZyme: GH109 No
- 17392 - 18120 (-) CDS No
- 18355 - 20769 (-) CDS No
- 20784 - 22100 (-) CDS No
- 22105 - 23862 (-) STP: STP|FecR No
- 23859 - 24311 (-) STP: STP|Sigma70_r2,STP|Sigma70_r4_2 No

Loading content...

PUL ID

PUL0782

PubMed

39738071, Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w.

Title

Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota.

Author

Perez-Cruz C, Moraleda-Montoya A, Liebana R, Terrones O, Arrizabalaga U, Garcia-Alija M, Lorizate M, Martinez Gascuena A, Garcia-Alvarez I, Nieto-Garai JA, Olazar-Intxausti J, Rodriguez-Colinas B, Mann E, Chiara JL, Contreras FX, Guerin ME, Trastoy B, Alonso-Saez L

Abstract

Marine brown algae produce the highly recalcitrant polysaccharide fucoidan, contributing to long-term oceanic carbon storage and climate regulation. Fucoidan is degraded by specialized heterotrophic bacteria, which promote ecosystem function and global carbon turnover using largely uncharacterized mechanisms. Here, we isolate and study two Planctomycetota strains from the microbiome associated with the alga Fucus spiralis, which grow efficiently on chemically diverse fucoidans. One of the strains appears to internalize the polymer, while the other strain degrades it extracellularly. Multi-omic approaches show that fucoidan breakdown is mediated by the expression of divergent polysaccharide utilization loci, and endo-fucanases of family GH168 are strongly upregulated during fucoidan digestion. Enzymatic assays and structural biology studies reveal how GH168 endo-fucanases degrade various fucoidan cores from brown algae, assisted by auxiliary hydrolytic enzymes. Overall, our results provide insights into fucoidan processing mechanisms in macroalgal-associated bacteria.