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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0774

PubMed

39738071, Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w.

Characterization method

RNA-seq,differential gene expression,HPAEC-PAD,SDS-PAGE,para-hydroxybenzoic acid (PAHBAH) assay,reducing-sugar assay,Carbohydrate Polyacrylamide Gel Electrophoresis (C-PAGE),enzyme activity assay

Genomic accession number

CAWLDL010000001.1

Nucelotide position range

8246839-8283270

Substrate

fucoidan

Loci

None

Species

Rhodopirellula sp. SWK7/595460

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 2400 (+) CAZyme: GH141 Yes
- 2784 - 4610 (+) other Yes
- 5456 - 6958 (+) other Yes
- 7069 - 8595 (+) CAZyme: GH29 Yes
- 8727 - 10568 (+) CAZyme: GH29 Yes
- 11171 - 11626 (-) other Yes
- 11735 - 11962 (-) other Yes
- 12167 - 13903 (+) CAZyme: GH168 Yes
- 14129 - 15547 (+) other Yes
- 15549 - 17693 (+) other Yes
- 17871 - 19727 (+) other Yes
- 19853 - 23575 (+) CAZyme: GH29 Yes
- 23535 - 23693 (+) other Yes
- 23725 - 25338 (+) other Yes
- 25350 - 26702 (+) CAZyme: GH168 Yes
- 26934 - 28046 (-) CAZyme: GH117 Yes
- 28399 - 29019 (+) TF: DBD-Pfam|GerE,DBD-SUPERFAMILY|0039939 Yes
- 29275 - 31242 (+) TC: gnl|TC-DB|Q9HC98|1.I.1.1.3 Yes
- 32433 - 32741 (+) other Yes
- 32834 - 33103 (-) other Yes
- 33042 - 33356 (-) other Yes
- 33413 - 33622 (+) other Yes
- 34429 - 36432 (+) CAZyme: GH95 Yes

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PUL ID

PUL0774

PubMed

39738071, Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w.

Title

Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota.

Author

Perez-Cruz C, Moraleda-Montoya A, Liebana R, Terrones O, Arrizabalaga U, Garcia-Alija M, Lorizate M, Martinez Gascuena A, Garcia-Alvarez I, Nieto-Garai JA, Olazar-Intxausti J, Rodriguez-Colinas B, Mann E, Chiara JL, Contreras FX, Guerin ME, Trastoy B, Alonso-Saez L

Abstract

Marine brown algae produce the highly recalcitrant polysaccharide fucoidan, contributing to long-term oceanic carbon storage and climate regulation. Fucoidan is degraded by specialized heterotrophic bacteria, which promote ecosystem function and global carbon turnover using largely uncharacterized mechanisms. Here, we isolate and study two Planctomycetota strains from the microbiome associated with the alga Fucus spiralis, which grow efficiently on chemically diverse fucoidans. One of the strains appears to internalize the polymer, while the other strain degrades it extracellularly. Multi-omic approaches show that fucoidan breakdown is mediated by the expression of divergent polysaccharide utilization loci, and endo-fucanases of family GH168 are strongly upregulated during fucoidan digestion. Enzymatic assays and structural biology studies reveal how GH168 endo-fucanases degrade various fucoidan cores from brown algae, assisted by auxiliary hydrolytic enzymes. Overall, our results provide insights into fucoidan processing mechanisms in macroalgal-associated bacteria.