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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0747

PubMed

38890895, Foods. 2024 May 26;13(11):1666. doi: 10.3390/foods13111666.

Characterization method

high performance gel permeation chromatography,gas chromatography,RNA-seq,differential gene expression

Genomic accession number

NZ_CP012938.1

Nucelotide position range

2036824-2056868

Substrate

pectic polysaccharide

Loci

Bovatus_RS08285-Bovatus_RS08335

Species

Bacteroides ovatus strain ATCC 8483/28116

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- Bovatus_RS08285 WP_004311434.1 0 - 1176 (-) NZ_CP012938.1:2036824-2038000 -
- Bovatus_RS08290 WP_004300809.1 1318 - 3136 (-) NZ_CP012938.1:2038142-2039960 -
- Bovatus_RS08295 WP_004300810.1 3280 - 6367 (-) NZ_CP012938.1:2040104-2043191 -
- Bovatus_RS08300 WP_004300811.1 6534 - 8859 (-) NZ_CP012938.1:2043358-2045683 -
- Bovatus_RS08305 WP_004300812.1 8877 - 10461 (-) NZ_CP012938.1:2045701-2047285 -
- Bovatus_RS08310 WP_004321470.1 10735 - 12550 (-) NZ_CP012938.1:2047559-2049374 -
- Bovatus_RS08315 WP_004300814.1 12572 - 15779 (-) NZ_CP012938.1:2049396-2052603 -
- Bovatus_RS08320 - 16077 - 16638 (-) NZ_CP012938.1:2052901-2053462 -
- Bovatus_RS08325 WP_004300816.1 16633 - 17254 (-) NZ_CP012938.1:2053457-2054078 -
- Bovatus_RS08335 WP_004300820.1 18062 - 20045 (-) NZ_CP012938.1:2054886-2056869 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1176 (-) TC: gnl|TC-DB|P0AAT4|1.A.23.4.5 Yes
- 1319 - 3136 (-) CAZyme: GH29|CBM32 Yes
- 3281 - 6367 (-) CAZyme: GH2 Yes
- 6535 - 8859 (-) CAZyme: GH20 Yes
- 8878 - 10461 (-) other Yes
- 10736 - 12550 (-) other Yes
- 12573 - 15779 (-) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 16634 - 17254 (-) CDS No
- 18063 - 20045 (-) STP: STP|HisKA,STP|HATPase_c,STP|Response_reg No

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PUL ID

PUL0747

PubMed

38890895, Foods. 2024 May 26;13(11):1666. doi: 10.3390/foods13111666.

Title

The Utilization by Bacteroides spp. of a Purified Polysaccharide from Fuzhuan Brick Tea.

Author

Shi J, Zhou W, Chen G, Yi W, Sun Y, Zeng X

Abstract

In the present study, four Bacteroides species that could degrade Fuzhuan brick tea polysaccharide-3 (FBTPS-3) were isolated from human feces and identified to be Bacteroides ovatus, B. uniformis, B. fragilis and B. thetaiotaomicron. The four Bacteroides species showed growth on FBTPS-3 as the carbon source, and B. ovatus showed the best capability for utilizing FBTPS-3 among the four species since B. ovatus could utilize more FBTPS-3 during 24 h fermentation. Moreover, the four Bacteroides species could metabolize FBTPS-3 and promote the production of acetic, propionic and isovaleric acids. Transcriptome analysis of B. ovatus revealed that 602 genes were up-regulated by FBTPS-3, including two carbohydrate-active enzyme clusters and four polysaccharide utilization loci (PULs). The PUL 1 contained GH28 family that could hydrolyze rhamnogalacturonan and other pectic substrates, which was in line with our previous work that rhamnose and galacturonic acid were the main component monosaccharides of FBTPS-3. Collectively, the results suggested that FBTPS-3 could be utilized by Bacteroides spp., and it might be developed as a promising prebiotic targeting Bacteroidetes in intestinal environment.