dbCAN-PUL

PUL ID	PUL0745
PubMed	38890895, Foods. 2024 May 26;13(11):1666. doi: 10.3390/foods13111666.
Characterization method	high performance gel permeation chromatography,gas chromatography,RNA-seq,differential gene expression
Genomic accession number	NZ_CP012938.1
Nucelotide position range	14804-35944
Substrate	pectic polysaccharide
Loci	Bovatus_RS00070-Bovatus_RS00125
Species	Bacteroides ovatus strain ATCC 8483/28116
Degradation or Biosynthesis	degradation

Cluster number	1
Gene name	Gene position	Gene type	Found by CGCFinder?
-	1 - 2745 (+)	TF: DBD-Pfam\|HTH_AraC,DBD-SUPERFAMILY\|0035607	No
-	2974 - 3384 (+)	CDS	No
-	3386 - 3916 (+)	TF: DBD-Pfam\|GerE	No
-	4014 - 7172 (+)	TC: gnl\|TC-DB\|Q45780\|1.B.14.6.1	Yes
-	7186 - 8940 (+)	other	Yes
-	8959 - 10482 (+)	other	Yes
-	10503 - 11861 (+)	other	Yes
-	11946 - 13373 (+)	CAZyme: GH30_2	Yes
-	13547 - 14935 (+)	CAZyme: GH28	Yes
-	14980 - 16911 (+)	CAZyme: GH146	Yes
-	16927 - 18852 (+)	other	Yes
-	18874 - 21141 (+)	CAZyme: PL12	Yes

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PUL ID	PUL0745
PubMed	38890895, Foods. 2024 May 26;13(11):1666. doi: 10.3390/foods13111666.
Title	The Utilization by Bacteroides spp. of a Purified Polysaccharide from Fuzhuan Brick Tea.
Author	Shi J, Zhou W, Chen G, Yi W, Sun Y, Zeng X
Abstract	In the present study, four Bacteroides species that could degrade Fuzhuan brick tea polysaccharide-3 (FBTPS-3) were isolated from human feces and identified to be Bacteroides ovatus, B. uniformis, B. fragilis and B. thetaiotaomicron. The four Bacteroides species showed growth on FBTPS-3 as the carbon source, and B. ovatus showed the best capability for utilizing FBTPS-3 among the four species since B. ovatus could utilize more FBTPS-3 during 24 h fermentation. Moreover, the four Bacteroides species could metabolize FBTPS-3 and promote the production of acetic, propionic and isovaleric acids. Transcriptome analysis of B. ovatus revealed that 602 genes were up-regulated by FBTPS-3, including two carbohydrate-active enzyme clusters and four polysaccharide utilization loci (PULs). The PUL 1 contained GH28 family that could hydrolyze rhamnogalacturonan and other pectic substrates, which was in line with our previous work that rhamnose and galacturonic acid were the main component monosaccharides of FBTPS-3. Collectively, the results suggested that FBTPS-3 could be utilized by Bacteroides spp., and it might be developed as a promising prebiotic targeting Bacteroidetes in intestinal environment.