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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0736

PubMed

38563787, Appl Environ Microbiol. 2024 May 21;90(5):e0004624. doi: 10.1128/aem.00046-24. Epub 2024 Apr 2.

Characterization method

RNA-seq,RT-qPCR,enzyme activity assay,thin-layer chromatography,Western Blot,recombinant protein expression,DSS-induced mouse colitis model

Genomic accession number

NZ_GL882595.1

Nucelotide position range

202523-218248

Substrate

alginate

Loci

HMPREF9445_RS08890-HMPREF9445_RS08935

Species

Bacteroides clarus YIT 12056/626929

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- HMPREF9445_RS08890 WP_009122070.1 0 - 714 (-) NZ_GL882595.1:202523-203237 -
- HMPREF9445_RS08895 WP_009122071.1 934 - 1891 (+) NZ_GL882595.1:203457-204414 -
- HMPREF9445_RS08900 WP_009122072.1 1965 - 2718 (-) NZ_GL882595.1:204488-205241 1.1.1.-
- HMPREF9445_RS08905 WP_004291742.1 2750 - 4217 (-) NZ_GL882595.1:205273-206740 -
- HMPREF9445_RS08910 WP_004291739.1 4222 - 4567 (-) NZ_GL882595.1:206745-207090 -
- HMPREF9445_RS08915 WP_009122073.1 4598 - 6761 (-) NZ_GL882595.1:207121-209284 -
- HMPREF9445_RS08920 WP_039950771.1 7266 - 10374 (+) NZ_GL882595.1:209789-212897 -
- HMPREF9445_RS08925 WP_229116276.1 10392 - 11838 (+) NZ_GL882595.1:212915-214361 -
- HMPREF9445_RS08930 WP_009122076.1 11843 - 13424 (+) NZ_GL882595.1:214366-215947 -
- HMPREF9445_RS08935 WP_009122077.1 13452 - 15726 (+) NZ_GL882595.1:215975-218249 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 714 (-) TF: DBD-Pfam|GntR,DBD-SUPERFAMILY|0039384 No
- 935 - 1891 (+) CDS No
- 1966 - 2718 (-) CDS No
- 2751 - 4217 (-) TC: gnl|TC-DB|Q07YH1|2.A.1.14.25 Yes
- 4223 - 4567 (-) other Yes
- 4599 - 6761 (-) CAZyme: PL17_2|PL17 Yes
- 7267 - 10374 (+) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 10393 - 11838 (+) TC: gnl|TC-DB|F9ZC67|8.A.46.1.2 Yes
- 11844 - 13424 (+) CAZyme: CE20 Yes
- 13453 - 15726 (+) CAZyme: PL6|PL6_1 Yes

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PUL ID

PUL0736

PubMed

38563787, Appl Environ Microbiol. 2024 May 21;90(5):e0004624. doi: 10.1128/aem.00046-24. Epub 2024 Apr 2.

Title

Alginate oligosaccharide assimilation by gut microorganisms and the potential role in gut inflammation alleviation.

Author

Ma X-Q, Wang B, Wei W, Tan F-C, Su H, Zhang J-Z, Zhao C-Y, Zheng H-J, Feng Y-Q, Shen W, Yang J-B, Li F-L

Abstract

Dietary fiber metabolism by gut microorganisms plays important roles in host physiology and health. Alginate, the major dietary fiber of daily diet seaweeds, is drawing more attention because of multiple biological activities. To advance the understanding of alginate assimilation mechanism in the gut, we show the presence of unsaturated alginate oligosaccharides (uAOS)-specific alginate utilization loci (AUL) in human gut microbiome. As a representative example, a working model of the AUL from the gut microorganism Bacteroides clarus was reconstructed from biochemistry and transcriptome data. The fermentation of resulting monosaccharides through Entner-Doudoroff pathway tunes the metabolism of short-chain fatty acids and amino acids. Furthermore, we show that uAOS feeding protects the mice against dextran sulfate sodium-induced acute colitis probably by remodeling gut microbiota and metabolome. IMPORTANCE: Alginate has been included in traditional Chinese medicine and daily diet for centuries. Recently discovered biological activities suggested that alginate-derived alginate oligosaccharides (AOS) might be an active ingredient in traditional Chinese medicine, but how these AOS are metabolized in the gut and how it affects health need more information. The study on the working mechanism of alginate utilization loci (AUL) by the gut microorganism uncovers the role of unsaturated alginate oligosaccharides (uAOS) assimilation in tuning short-chain fatty acids and amino acids metabolism and demonstrates that uAOS metabolism by gut microorganisms results in a variation of cell metabolites, which potentially contributes to the physiology and health of gut.