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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0729

PubMed

38357353, Front Microbiol. 2024 Jan 31;15:1160472. doi: 10.3389/fmicb.2024.1160472. eCollection 2024.

Characterization method

RT-PCR,gene mutant,enzyme activity assay

Genomic accession number

NC_020238.1

Nucelotide position range

5336873-5346552

Substrate

cellobiose

Loci

HD73_RS27950-HD73_RS27990

Species

Bacillus thuringiensis serovar kurstaki str. HD73/1428

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- HD73_RS27950 WP_000820970.1 0 - 2676 (+) NC_020238.1:5336873-5339549 -
exsM HD73_RS27955 WP_000207046.1 2980 - 3394 (-) NC_020238.1:5339853-5340267 -
- HD73_RS27960 WP_000856213.1 3581 - 4763 (+) NC_020238.1:5340454-5341636 -
chbG HD73_RS27965 WP_000593487.1 4801 - 5506 (-) NC_020238.1:5341674-5342379 3.5.1.105
celF HD73_RS27970 WP_000145762.1 5555 - 6881 (-) NC_020238.1:5342428-5343754 3.2.1.86
- HD73_RS27975 WP_000989059.1 6884 - 7208 (-) NC_020238.1:5343757-5344081 -
celB HD73_RS27980 WP_001168848.1 7389 - 8697 (-) NC_020238.1:5344262-5345570 2.7.1.205
- HD73_RS27985 WP_001023567.1 8713 - 9016 (-) NC_020238.1:5345586-5345889 2.7.1.-
- HD73_RS27990 WP_000379645.1 9362 - 9680 (-) NC_020238.1:5346235-5346553 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 2676 (+) TF: DBD-Pfam|MarR,DBD-SUPERFAMILY|0043095 No
- 2981 - 3394 (-) CDS No
- 3582 - 4763 (+) TC: gnl|TC-DB|P14285|2.A.51.1.3 Yes
- 4802 - 5506 (-) other Yes
celF 5556 - 6881 (-) CAZyme: GH4 Yes
- 6885 - 7208 (-) TC: gnl|TC-DB|Q72XQ1|4.A.3.2.8 Yes
celB 7390 - 8697 (-) TC: gnl|TC-DB|P46317|4.A.3.2.2 Yes
- 8714 - 9016 (-) TC: gnl|TC-DB|Q72XP9|4.A.3.2.8 Yes
- 9363 - 9680 (-) TC: gnl|TC-DB|Q72XQ1|4.A.3.2.8 Yes

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PUL ID

PUL0729

PubMed

38357353, Front Microbiol. 2024 Jan 31;15:1160472. doi: 10.3389/fmicb.2024.1160472. eCollection 2024.

Title

Transcriptional regulation of cellobiose utilization by PRD-domain containing Sigma54-dependent transcriptional activator (CelR) and catabolite control protein A (CcpA) in Bacillus thuringiensis.

Author

Zhang L, Xu H, Cheng H, Song F, Zhang J, Peng Q

Abstract

Cellobiose, a beta-1,4-linked glucose dimer, is a major cellodextrin resulting from the enzymatic hydrolysis of cellulose. It is a major source of carbon for soil bacteria. In bacteria, the phosphoenolpyruvate (PEP): carbohydrate phosphotransferase system (PTS), encoded by the cel operon, is responsible for the transport and utilization of cellobiose. In this study, we analyzed the transcription and regulation of the cel operon in Bacillus thuringiensis (Bt). The cel operon is composed of five genes forming one transcription unit. beta-Galactosidase assays revealed that cel operon transcription is induced by cellobiose, controlled by Sigma54, and positively regulated by CelR. The HTH-AAA(+) domain of CelR recognized and specifically bound to three possible binding sites in the celA promoter region. CelR contains two PTS regulation domains (PRD1 and PRD2), which are separated by two PTS-like domains-the mannose transporter enzyme IIA component domain (EIIA(Man)) and the galactitol transporter enzyme IIB component domain (EIIB(Gat)). Mutations of His-546 on the EIIA(Man) domain and Cys-682 on the EIIB(Gat) domain resulted in decreased transcription of the cel operon, and mutations of His-839 on PRD2 increased transcription of the cel operon. Glucose repressed the transcription of the cel operon and catabolite control protein A (CcpA) positively regulated this process by binding the cel promoter. In the celABCDE and celR mutants, PTS activities were decreased, and cellobiose utilization was abolished, suggesting that the cel operon is essential for cellobiose utilization. Bt has been widely used as a biological pesticide. The metabolic properties of Bt are critical for fermentation. Nutrient utilization is also essential for the environmental adaptation of Bt. Glucose is the preferred energy source for many bacteria, and the presence of the phosphotransferase system allows bacteria to utilize other sugars in addition to glucose. Cellobiose utilization pathways have been of particular interest owing to their potential for developing alternative energy sources for bacteria. The data presented in this study improve our understanding of the transcription patterns of cel gene clusters. This will further help us to better understand how cellobiose is utilized for bacterial growth.