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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0720

PubMed

24385433, J Biol Chem. 2014 Feb 21;289(8):5240-9. doi: 10.1074/jbc.M113.528711. Epub 2014 Jan 2.

Characterization method

HPAEC-PAD,enzyme kinetic analysis,thin-layer chromatography,recombinant protein expression,gene mutant

Genomic accession number

NC_015067.1

Nucelotide position range

266861-284077

Substrate

arabinan

Loci

BLLJ_RS01065-BLLJ_RS01100

Species

 Bifidobacterium longum JCM 1217/216816

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- BLLJ_RS01065 WP_007054733.1 0 - 1023 (-) NC_015067.1:266861-267884 -
- BLLJ_RS01075 WP_007051556.1 1508 - 2834 (+) NC_015067.1:268369-269695 -
- BLLJ_RS01080 WP_007054735.1 3121 - 4048 (+) NC_015067.1:269982-270909 -
- BLLJ_RS01085 WP_007051554.1 4068 - 4887 (+) NC_015067.1:270929-271748 -
hypBA1 BLLJ_RS01090 WP_013582351.1 4889 - 6866 (+) NC_015067.1:271750-273727 3.2.1.185
hypBA2 BLLJ_RS01095 WP_013582352.1 7564 - 13396 (+) NC_015067.1:274425-280257 3.2.1.187
- BLLJ_RS01100 WP_013582353.1 13500 - 17217 (+) NC_015067.1:280361-284078 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1023 (-) TF: DBD-Pfam|LacI,DBD-SUPERFAMILY|0036955 No
- 1509 - 2834 (+) STP: STP|SBP_bac_1 No
- 3122 - 4048 (+) TC: gnl|TC-DB|O51924|3.A.1.1.7 Yes
- 4069 - 4887 (+) TC: gnl|TC-DB|Q72H66|3.A.1.1.25 Yes
hypBA1 4890 - 6866 (+) CAZyme: GH127 Yes
hypBA2 7565 - 13396 (+) CAZyme: GH121 Yes
- 13501 - 17217 (+) CAZyme: GH43_29 Yes

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PUL ID

PUL0720

PubMed

24385433, J Biol Chem. 2014 Feb 21;289(8):5240-9. doi: 10.1074/jbc.M113.528711. Epub 2014 Jan 2.

Title

Characterization of a novel beta-L-arabinofuranosidase in Bifidobacterium longum: functional elucidation of a DUF1680 protein family member.

Author

Fujita K, Takashi Y, Obuchi E, Kitahara K, Suganuma T

Abstract

Pfam DUF1680 (PF07944) is an uncharacterized protein family conserved in many species of bacteria, actinomycetes, fungi, and plants. Previously, we cloned and characterized the hypBA2 gene as a beta-L-arabinobiosidase in Bifidobacterium longum JCM 1217. In this study, we cloned a DUF1680 family member, the hypBA1 gene, which constitutes a gene cluster with hypBA2. HypBA1 is a novel beta-L-arabinofuranosidase that liberates L-arabinose from the L-arabinofuranose (Araf)-beta1,2-Araf disaccharide. HypBA1 also transglycosylates 1-alkanols with retention of the anomeric configuration. Mutagenesis and azide rescue experiments indicated that Glu-338 is a critical residue for catalytic activity. This study provides the first characterization of a DUF1680 family member, which defines a new family of glycoside hydrolases, the glycoside hydrolase family 127.