Home Repository Download Help About Statistics Taxonomy Blastx
Links
PULDB
CAZy
dbCAN2
dbCAN-seq
run_dbcan
CAZypedia
PlantCAZyme
Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0714

PubMed

37500984, Cell Mol Life Sci. 2023 Jul 28;80(8):232. doi: 10.1007/s00018-023-04812-w.

Characterization method

clone and expression,crystallization,isothermal titration calorimetry (ITC),thin-layer chromatography,Western Blot,gene mutant

Genomic accession number

NZ_CP012938.1

Nucelotide position range

4902937-4918992

Substrate

starch

Loci

Bovatus_RS18560-Bovatus_RS18595

Species

Bacteroides ovatus strain ATCC 8483 /28116

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- Bovatus_RS18560 WP_004299752.1 0 - 2277 (-) NZ_CP012938.1:4902937-4905214 -
- Bovatus_RS18565 WP_004305043.1 2440 - 3922 (-) NZ_CP012938.1:4905377-4906859 -
- Bovatus_RS18570 WP_004299754.1 3947 - 5120 (-) NZ_CP012938.1:4906884-4908057 -
susD Bovatus_RS18575 WP_004299755.1 5155 - 6766 (-) NZ_CP012938.1:4908092-4909703 -
- Bovatus_RS18580 WP_004299756.1 6795 - 9843 (-) NZ_CP012938.1:4909732-4912780 -
- Bovatus_RS18585 WP_004299757.1 9948 - 12165 (-) NZ_CP012938.1:4912885-4915102 3.2.1.-
- Bovatus_RS18590 WP_004299758.1 12362 - 14216 (-) NZ_CP012938.1:4915299-4917153 3.2.1.-
- Bovatus_RS18595 WP_004305037.1 14460 - 16056 (+) NZ_CP012938.1:4917397-4918993 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 2277 (-) CAZyme: GH13_10 Yes
- 2441 - 3922 (-) other Yes
- 3948 - 5120 (-) other Yes
susD 5156 - 6766 (-) TC: gnl|TC-DB|Q8A1G2|8.A.46.1.1 Yes
- 6796 - 9843 (-) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 9949 - 12165 (-) CAZyme: GH97 Yes
- 12363 - 14216 (-) CAZyme: GH13_46 Yes
- 14461 - 16056 (+) CDS No

Loading content...

PUL ID

PUL0714

PubMed

37500984, Cell Mol Life Sci. 2023 Jul 28;80(8):232. doi: 10.1007/s00018-023-04812-w.

Title

BoGH13A(Sus) from Bacteroides ovatus represents a novel alpha-amylase used for Bacteroides starch breakdown in the human gut.

Author

Brown HA, DeVeaux AL, Juliano BR, Photenhauer AL, Boulinguiez M, Bornschein RE, Wawrzak Z, Ruotolo BT, Terrapon N, Koropatkin NM

Abstract

Members of the Bacteroidetes phylum in the human colon deploy an extensive number of proteins to capture and degrade polysaccharides. Operons devoted to glycan breakdown and uptake are termed polysaccharide utilization loci or PUL. The starch utilization system (Sus) is one such PUL and was initially described in Bacteroides thetaiotaomicron (Bt). BtSus is highly conserved across many species, except for its extracellular alpha-amylase, SusG. In this work, we show that the Bacteroides ovatus (Bo) extracellular alpha-amylase, BoGH13A(Sus), is distinguished from SusG in its evolutionary origin and its domain architecture and by being the most prevalent form in Bacteroidetes Sus. BoGH13A(Sus) is the founding member of both a novel subfamily in the glycoside hydrolase family 13, GH13_47, and a novel carbohydrate-binding module, CBM98. The BoGH13A(Sus) CBM98-CBM48-GH13_47 architecture differs from the CBM58 embedded within the GH13_36 of SusG. These domains adopt a distinct spatial orientation and invoke a different association with the outer membrane. The BoCBM98 binding site is required for Bo growth on polysaccharides and optimal enzymatic degradation thereof. Finally, the BoGH13A(Sus) structure features bound Ca(2+) and Mn(2+) ions, the latter of which is novel for an alpha-amylase. Little is known about the impact of Mn(2+) on gut bacterial function, much less on polysaccharide consumption, but Mn(2+) addition to Bt expressing BoGH13A(Sus) specifically enhances growth on starch. Further understanding of bacterial starch degradation signatures will enable more tailored prebiotic and pharmaceutical approaches that increase starch flux to the gut.