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PlantCAZyme
Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0681

PubMed

35110564, Nat Commun. 2022 Feb 2;13(1):629. doi: 10.1038/s41467-022-28310-y.

Characterization method

enzyme activity assay,NMR

Genomic accession number

JABUTP010000015.1

Nucelotide position range

7177-23063

Substrate

pectic polysaccharide

Loci

HUJ91_01905-HUJ91_01950

Species

Bacteroidaceae bacterium/2212467

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- HUJ91_01905 MCF0172478.1 0 - 1787 (-) JABUTP010000015.1:7177-8964 -
- HUJ91_01910 MCF0172479.1 1847 - 2548 (-) JABUTP010000015.1:9024-9725 -
- HUJ91_01915 MCF0172480.1 2568 - 4077 (-) JABUTP010000015.1:9745-11254 -
- HUJ91_01920 MCF0172481.1 4088 - 6485 (-) JABUTP010000015.1:11265-13662 -
- HUJ91_01925 MCF0172482.1 6523 - 7738 (-) JABUTP010000015.1:13700-14915 -
- HUJ91_01930 MCF0172483.1 7810 - 9148 (-) JABUTP010000015.1:14987-16325 -
- HUJ91_01935 MCF0172484.1 9181 - 11077 (-) JABUTP010000015.1:16358-18254 -
- HUJ91_01940 MCF0172485.1 11089 - 13972 (-) JABUTP010000015.1:18266-21149 -
- HUJ91_01945 MCF0172486.1 13997 - 14173 (-) JABUTP010000015.1:21174-21350 -
- HUJ91_01950 MCF0172487.1 14182 - 15887 (-) JABUTP010000015.1:21359-23064 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1787 (-) CAZyme: GH2 Yes
- 1848 - 2548 (-) other Yes
- 2569 - 4077 (-) CAZyme: GH173 Yes
- 4089 - 6485 (-) CAZyme: GH2 Yes
- 6524 - 7738 (-) CAZyme: GH5_57 Yes
- 7811 - 9148 (-) other Yes
- 9182 - 11077 (-) other Yes
- 11090 - 13972 (-) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 13998 - 14173 (-) other Yes
- 14183 - 15887 (-) CAZyme: GH78 Yes

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PUL ID

PUL0681

PubMed

35110564, Nat Commun. 2022 Feb 2;13(1):629. doi: 10.1038/s41467-022-28310-y.

Title

Gut microbiome of the largest living rodent harbors unprecedented enzymatic systems to degrade plant polysaccharides.

Author

Cabral L, Persinoti GF, Paixao DAA, Martins MP, Morais MAB, Chinaglia M, Domingues MN, Sforca ML, Pirolla RAS, Generoso WC, Santos CA, Maciel LF, Terrapon N, Lombard V, Henrissat B, Murakami MT

Abstract

The largest living rodent, capybara, can efficiently depolymerize and utilize lignocellulosic biomass through microbial symbiotic mechanisms yet elusive. Herein, we elucidate the microbial community composition, enzymatic systems and metabolic pathways involved in the conversion of dietary fibers into short-chain fatty acids, a main energy source for the host. In this microbiota, the unconventional enzymatic machinery from Fibrobacteres seems to drive cellulose degradation, whereas a diverse set of carbohydrate-active enzymes from Bacteroidetes, organized in polysaccharide utilization loci, are accounted to tackle complex hemicelluloses typically found in gramineous and aquatic plants. Exploring the genetic potential of this community, we discover a glycoside hydrolase family of beta-galactosidases (named as GH173), and a carbohydrate-binding module family (named as CBM89) involved in xylan binding that establishes an unprecedented three-dimensional fold among associated modules to carbohydrate-active enzymes. Together, these results demonstrate how the capybara gut microbiota orchestrates the depolymerization and utilization of plant fibers, representing an untapped reservoir of enzymatic mechanisms to overcome the lignocellulose recalcitrance, a central challenge toward a sustainable and bio-based economy.