LOCUS       NZ_CAWLDM010000001     12144 bp    DNA     linear   CON 20-NOV-2024
DEFINITION  Neorhodopirellula lusitana isolate 913.contigs
            NODE_1_length_7962739_cov_92.144000, whole genome shotgun sequence.
ACCESSION   NZ_CAWLDM010000001 REGION: 6897268..6909411
VERSION     NZ_CAWLDM010000001.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA115022804
            Assembly: GCF_963859645.1
KEYWORDS    WGS; RefSeq.
SOURCE      Neorhodopirellula lusitana
  ORGANISM  Neorhodopirellula lusitana
            Bacteria; Pseudomonadati; Planctomycetota; Planctomycetia;
            Pirellulales; Pirellulaceae; Neorhodopirellula.
REFERENCE   1  (bases 1 to 12144)
  AUTHORS   Liebana Garcia,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2023) AZTI, Marine Microbiology, Txatxarramendi
            ugartea z/g 48395 Sukarrieta - Bizkaia, Spain
REFERENCE   2  (bases 1 to 12144)
  AUTHORS   Liebana Garcia,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2023) AZTI, Marine Microbiology, Txatxarramendi
            ugartea z/g 48395 Sukarrieta - Bizkaia, Spain
COMMENT     REFSEQ INFORMATION: The reference sequence is identical to
            CAWLDM010000001.1.
            The genome was assembled using a hybrid assembly approach with
            Spades (1 contig > 5Kbp, coverage 92).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_963859645.1-RS_2024_11_13
            Annotation Date                   :: 11/13/2024 07:55:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,800
            CDSs (total)                      :: 5,709
            Genes (coding)                    :: 5,662
            CDSs (with protein)               :: 5,662
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 10 of 48
            Pseudo Genes (incomplete)         :: 37 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12144
                     /organism="Neorhodopirellula lusitana"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1_length_7962739_cov_92.144000"
                     /isolate="913.contigs"
                     /isolation_source="Fucus spiralis biofilm"
                     /db_xref="taxon:445327"
                     /note="contig: NODE_1_length_7962739_cov_92.144000"
     gene            1..1623
                     /locus_tag="ACI5KW_RS25295"
     CDS             1..1623
                     /locus_tag="ACI5KW_RS25295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008701889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="WP_404310364.1"
                     /translation="MSYFTISCPGCKKHLKVRDALAGKSRACPHCRVSFRVPDSASDS
                     KVTPATLNSTQTPTKPQPSPTPDAGLEINTQPKIDTSGTAKPKPVKHHRTAKKGWFDS
                     SSESASSDVSLVISGILGAVMTVLWFALLYPFQDNYFGALFWQRGPVPFPTTFLMFWA
                     VAILILKWRRLQEQRNAMLLDVLPLEVSSEITFGSIDEFIGHINDLPGASSDSFLINR
                     VVRGIEHFRVRKSAAETATMMESQSAIDANNIAGSYTILKVFIWALPILGFIGTVMGV
                     SSAVASLASSLAGGGSMDAMKSALQDVFGGLGTAFDTTLLALIMSMLVKIPASALQKS
                     EEDLITNVDEYCNENLLRRLNDDREGGAERSGSGGTGSRDTKIFRDAVEQALGTQHAE
                     MERWLEKLDAIGARMTKQVSQGWDEVNDRIESQQEKHVQTLHTQQLDQQANLQAQLDQ
                     MANAADKIQSTLNTLAEQTASMQTQVNDTFAQTHNTLREHLGGVQTGLSSLSQVLENL
                     GEHQVVIQQVESNQPRRAWFGGRGRKSRNGRA"
     gene            1626..2438
                     /locus_tag="ACI5KW_RS25300"
     CDS             1626..2438
                     /locus_tag="ACI5KW_RS25300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008701887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_404310365.1"
                     /translation="MPRRPKSNDDDISLFPFLSIIASVIGVLTMMIATLALAQTDKPD
                     VAQIEAYEDSQKELKASEERIAELKQEISVAQSDSLEIQEQRQQLTVTVAELKKLFEE
                     LQQVEQELAAQKEVVIVIPTLDKQMRESLDDMQNQAEELKEQIAQLESNLDERTNPKE
                     ANVTILPQGSGQNFQPHFIECADGALVLHTSENPKRIPAGAMTKDADFLAVLNEVANG
                     KDDTLVLLIRSDGLNTYRAVKRLCDQREIRNGKLPIVGKGRVDLSAFTGTQP"
     gene            2435..3568
                     /locus_tag="ACI5KW_RS25305"
     CDS             2435..3568
                     /locus_tag="ACI5KW_RS25305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_404310366.1"
                     /translation="MRKPRDDGEDDGGLDSLLDTMTNVVGILVLVLIVTQMSVADVVT
                     RITTENKIDENAVQELQKELEQLQEQESELEQVLINPDSVNIDRQREELRRQKELLQR
                     RKELIKQREKEKNEYSIKIEQDKKQADQSQQQVEAAQNKREELETLITESLQKKATLE
                     AKLARTPKTEAPADIEVSIPDPRPAPPGAKELTVLCTGDEIFLVNFDAFRETAKKRAM
                     GLAITTTSKREPEKGIDVKKFTERWERMKDQDDFFDVEYYVQNERFPRLRMKPRKGRG
                     ASAAELLNPRSRVRKAWLSQIDITKYYARFNVMPDSFAVYAVARRFFTESGMLSGWTP
                     QSDSYELTSWVPGGMELGPPRPKPPPKPTDPNASPPKPPNVID"
     gene            3812..5254
                     /locus_tag="ACI5KW_RS25310"
     CDS             3812..5254
                     /locus_tag="ACI5KW_RS25310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="WP_404310367.1"
                     /translation="MTRWILCLGLLLTPAIASADQTKTNFVFLLVDDLGWGDFGSYGA
                     EFYETPNIDNLASQGMQFSNAYAACTVCSPSRAAILTGCYPARTHLTDWIAGHNHPKA
                     KLSVPDWTMKMAHSRVLLPEALHEAGYSTAFFGKWHLMPIGAPDFDEHYPTAHGFDLN
                     VGGREWGQPKGPGKYFSPFGMPNLDDGKPGDFLTDKLTDAAVDFLDNTTRDQPFLLYF
                     SYYTLHGPIMCPPELEAKYTQKAKTFQNSKNEHLNAKRAGMVECLDNSVGRLMQKLND
                     LGIADNTVIILTGDNGGDYDKSTGGLRDYKGYSHEGAVREPLIVRWPGKTSAGSTCDE
                     TVIGTDFYPTMLEMAGLPPRDSEHLDGKSIVPLLTGSSTELDRDTVYWHYPHYHRTKP
                     YGAIREGDWKLIEFFEDGRLELFNLKEDEQEANDLAESNPEKAQRLLKKLQAWRTSVD
                     AQMMTDNPAYDPATADKKKRRPKQKQPAKS"
     gene            complement(5409..6920)
                     /locus_tag="ACI5KW_RS25315"
     CDS             complement(5409..6920)
                     /locus_tag="ACI5KW_RS25315"
                     /EC_number="3.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008680180.1"
                     /GO_function="GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="WP_283431926.1"
                     /translation="MFVKIITPLILAGLLQTCVCAQQTASVATKGKAITDEAAAWKAI
                     DGRDTPQWWKDAKFGIFIHWGPYSVPAFSEVGRYSEWYWEDLMNPKRRSHKAVKAFHE
                     KNYGKDFKYEEFADQFKCELFDPAQWAEAFEESGAKYVVLTSKHHDGYCLWPNEQADK
                     SFGRPWCSTNSGPMRDLVGELTEEVRKTDVKMGLYFSIYEWYNPLYKTKLNSFVDDHL
                     IPQFKDVVNKYEPAVIFSDGEWDHPAADWKSAEMLTWLFNESPCKDEVVINDRWGKGD
                     RHKHGGYYTTEYGAGLPDGTNAWEENRGMAHSFGYSRTERFEDYNSSESLILMLADIV
                     SRGGNFLLDIGPTADGRIPPLMEERLSEIGDWLDVNGEAIYGTTTHSSTCQWSTGKAQ
                     ELGRGNYRVKYDVEKLMKSPEEGMARKELLFTRKGETLYAICALLPDETMTLHHVQAA
                     EGATVSMLGVGELEWEQSGEDLIIKMPKLNPSKMPCEHAWTFKVSKIQLHDAS"
     gene            complement(7058..9598)
                     /locus_tag="ACI5KW_RS25320"
     CDS             complement(7058..9598)
                     /locus_tag="ACI5KW_RS25320"
                     /inference="COORDINATES: protein motif:HMM:NF046196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 95 catalytic
                     domain-containing protein"
                     /protein_id="WP_404310368.1"
                     /translation="MAHPRINFFVIGFLVLFWAGNGSSGWADQPTPLDGVTTAEVAPE
                     QASQTKGDRAATRDYVLTSGQPSTNWGDALISGNGRQGVMVHGQPLEETIVLNHEKLW
                     VPAQPVKPDVPNMVSAIKQARELAKQEKWGPAASIVFNEFGKRNAEMFPEEALIRPGP
                     RFGLNYVHPALYFKISVPKKGEVKNYSRTLQLDSGEVSVRWTDDRGDWQRDIFVSRPD
                     NVVVTRLRRPSNASLTAELRFAARPGWNEEDIAAPVVEHIEGAEDGDGEMYLHTSYRH
                     LHGLPQAEGYQVMSRVIVKGGASHAKSDRILIQDVDEVLVLTRTEFLPIASQPSRDRL
                     RADLDELSESYVELLAAHAKVHGEMFHRVHYRLGGEFGNGRTTEEIIAEAEANGPTPA
                     YLEWIFAVGRYSLISSSGELPPTLMGIWGDTWKPMWWGHYTNDSNLNLAISNGSVGNL
                     PEAMESYFSWIETLYPDWERNAQQLYGARGYMGAIAHGWRHGVAIAGWQGWTGATGWL
                     GAYFWQHYLFTGDREFLAERVVPLLENTVLFYQDFLGPMEGRDGKFLIYPSISPENQS
                     PNTPGAPNATSEIAIIRGSVNALIDAYRELEINQDRIPELQAFLKKVPEYRINEDGAI
                     AEWSCPGVEDNYNHRHNSHLHAVYPGVEINPSTPELYQAARRAIEKRLESGQGNKSAH
                     GFMELGFYGSRLQDPEIVWNMLEDYARSKFIYRSFISSHNPDHRIYNLDSILALPAIL
                     TEMCVYSRPGEINLLPGIPATQLPRGEISGVLARKAMVIDRLAWDLPENTIRLELSSK
                     VDQSVAISSRLPIESVHSSAATFDGAQWRLALPANQSVSALIMLEKPN"
     gene            complement(9818..11590)
                     /locus_tag="ACI5KW_RS25325"
     CDS             complement(9818..11590)
                     /locus_tag="ACI5KW_RS25325"
                     /inference="COORDINATES: protein motif:HMM:NF024777.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="WP_404310369.1"
                     /translation="MISANFEELVQRYCSNVIDADQFDLLQDTLRESPEARKEFRRVL
                     RLHAALHELTDASHDNVSELVGNAPEVDASKPMSPPKLVPLGVDSQEEGIVYSNASGV
                     GWVLFACAAVVLFALGAQTFLARAPRDVAWQPSSDADREGVYYEEDAASGSQKITRTS
                     AHTVVNAPRFDRAVANLNVAVLRDSGTAKWVTPNRGIQIGDMLGPQTIELSEGVVELG
                     FLSGATAIIEGPAKIELVSRMSGLLFYGKVRCHVPPSASGFTIQSKETKYVDLGTDFA
                     LETTRDGRQELHVFDGEVEVRSSRGDVPVQRITSGNGLKQVEDRQQRIDSDAQQFPSS
                     NELRLLKESSEVKRHQEWRAYRDELSSDPSLLVFYDFQHKDDHPYELNNLCSDSVDGS
                     VIGCRWTQGRWKGKNALEFKRPDDRVRFEVPGEFERLTMSAWMRIDGFDRHFNSIMLT
                     DAYENGDIHWQIRSSGSIDTGIKPPGEDRVICVSKPVFGYDDLGRWFHIAAVADSQTT
                     EVRHYVNGELIGMSPYYDGELPKGEYKFRIGKAELGNWSPKRHYDEWPIRNLNGQIDE
                     FAVFDRALSPAEIEQLYNAGKPAS"
     gene            complement(11587..12144)
                     /locus_tag="ACI5KW_RS25330"
     CDS             complement(11587..12144)
                     /locus_tag="ACI5KW_RS25330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687869.1"
                     /GO_function="GO:0003700 - DNA-binding transcription
                     factor activity [Evidence IEA]; GO:0016987 - sigma factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]; GO:0006355 - regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="WP_283431929.1"
                     /translation="MDNQAGAGGDVARFVRLFTANERRIRAFLYGLLVNRDAVDEVMQ
                     NVSVVLWEKFSALQDDDGFLKWSYVVARYEAQMHRRKLARNRFVFDDDLLAQLASEYA
                     SEEDFQADDQMLHYLDDCMTKLDDSERELIVTAYGTNQSLTSVADRLDVTANSLYKTV
                     ARLRRRLQRCIDHKCQPVTPALGNE"
ORIGIN      
        1 atgtcttact tcacgatttc ctgcccagga tgcaagaagc atctaaaagt tcgagatgct
       61 ctcgctggta aaagccgagc ctgcccgcac tgccgggttt cgtttcgtgt tccggattcc
      121 gcgtccgatt cgaaagtcac gcccgccacc ctgaactcga ctcaaacacc gaccaagccc
      181 cagccctcac cgaccccaga cgccggtctg gagatcaaca ctcagccgaa gattgatacg
      241 agcgggacgg cgaaaccgaa accggtgaag catcaccgta ccgccaaaaa gggctggttt
      301 gattcttcca gcgagtccgc cagcagtgat gtctcactgg taataagcgg catccttggt
      361 gccgtaatga cggtgctgtg gtttgccttg ctctatccat tccaagacaa ctacttcggc
      421 gcgctgttct ggcaacgggg tcctgttccg ttcccaacca cattcctgat gttttgggcc
      481 gtcgccattt tgattctgaa atggcgacga ctccaagagc agcggaacgc gatgctactc
      541 gatgtccttc cactcgaagt ttcatccgag atcacgtttg gctccatcga tgaattcatc
      601 ggccatatca atgatctccc cggggccagc agcgacagtt tcttgatcaa ccgtgtcgtc
      661 cgcggaatcg aacatttccg cgtccgcaaa agtgccgccg aaacggccac aatgatggaa
      721 tctcaatcgg ccatcgacgc caacaatatt gcgggcagct acaccatctt gaaggttttc
      781 atctgggctc ttcccattct cggtttcatt ggaaccgtga tgggagttag ttccgcggta
      841 gccagcctag ccagcagtct cgctggcggc ggcagcatgg atgcgatgaa gtctgcactc
      901 caggacgtct ttggcggcct cggaaccgca tttgacacaa cattgctcgc actgatcatg
      961 agcatgctgg tcaagatccc cgcatccgcg ctccagaaaa gcgaagaaga tctgatcacc
     1021 aatgtcgatg agtactgcaa tgaaaatctg ctccgtcgac tcaacgatga ccgtgaaggt
     1081 ggcgccgaac gcagcggttc cggtgggacc ggttcacgcg ataccaagat attccgcgac
     1141 gcagtcgaac aagccctcgg cacccaacat gccgaaatgg agcgttggct tgaaaagctc
     1201 gacgcgatcg gcgctcgcat gaccaagcaa gtctcccaag gttgggacga ggtcaatgat
     1261 cgcatcgaat cccaacaaga aaaacatgtt caaaccctcc atacccagca gctcgatcaa
     1321 caagcgaacc tgcaagccca gctagaccag atggccaacg ccgccgacaa gatccaatcc
     1381 actctcaaca cgctggccga gcaaacggca tccatgcaaa ctcaggtgaa tgacaccttc
     1441 gcccaaaccc ataacactct tcgtgagcat ctcggcggcg ttcaaacagg cctctccagt
     1501 ctcagccaag tactggaaaa tctcggtgaa caccaagtcg tcatccaaca agtcgagtcc
     1561 aaccaacctc gtcgagcttg gttcggtggc cgcggacgca agagccgaaa cgggagggct
     1621 tagcaatgcc aagacggccc aagtccaacg acgacgacat ctcgctgttc ccgttcctta
     1681 gtattatcgc atccgtcatc ggcgtattga cgatgatgat cgcgacgctg gcactggccc
     1741 aaaccgacaa acccgatgtc gcacaaatcg aagcctacga agactctcaa aaagaattga
     1801 aagcgtccga agaacgcatt gctgaactca aacaagaaat ctcggtcgcc caatccgatt
     1861 cgctcgagat ccaagaacaa cggcaacaat tgaccgtgac ggtagccgaa ctcaaaaaac
     1921 tgttcgaaga actacagcaa gtcgaacaag aactcgccgc gcaaaaagaa gtcgtcatcg
     1981 tcatcccaac gcttgataag cagatgcgag aatcgctgga tgacatgcag aatcaggcgg
     2041 aagaactcaa ggaacaaatc gctcaacttg aatcgaacct tgacgagcgc accaatccga
     2101 aagaagccaa tgtgacgatc ctgccgcaag gtagcggcca aaacttccaa cctcatttca
     2161 tcgaatgtgc cgacggagca ctggtactgc acacgtcgga gaatccgaag cgaattcccg
     2221 ccggcgccat gaccaaggac gcagacttct tggcagtctt gaatgaagtt gccaacggaa
     2281 aagacgacac gctcgtgctg ttaattcgca gcgatggttt gaacacctac cgggcagtga
     2341 aacgactttg cgaccaacgt gaaatccgta acggcaagct acccattgtc ggtaaaggcc
     2401 gcgttgactt aagtgcattt acgggaaccc aaccatgaga aaaccacgcg acgatggaga
     2461 agacgacggt gggctcgact cacttttgga taccatgacc aacgtggtcg gaatcctggt
     2521 attagtcttg atcgtgactc aaatgagcgt tgcggatgtc gtaaccagaa tcacaaccga
     2581 aaacaaaatc gacgaaaatg cagtccaaga attgcaaaag gaactcgaac aactgcagga
     2641 acaggaatcc gaactcgaac aggtattgat caaccccgac agcgtcaata tcgaccgtca
     2701 acgtgaagaa ctgcgccgcc agaaagagct attgcaacgt cgcaaggaac tcatcaagca
     2761 acgcgaaaaa gaaaagaacg aatactccat caagattgaa caagacaaga aacaggcgga
     2821 ccaaagccag caacaggtcg aagcggcaca aaataagcgt gaagaactag aaacactgat
     2881 cacagagtca ttgcagaaga aagcgacgct cgaagccaaa ctagctcgca cacccaagac
     2941 cgaagcgcca gccgatatcg aggtcagcat tcccgacccg cgccccgcac ccccaggagc
     3001 gaaagaattg accgtactct gcacgggcga tgaaatcttc ctagtcaact ttgatgcttt
     3061 tcgagaaacc gctaagaaac gagcgatggg cctcgccatc acgaccactt ccaaacgaga
     3121 gcccgaaaaa gggatcgacg tcaagaagtt caccgaacgt tgggaacgga tgaaagacca
     3181 agacgacttc ttcgacgtgg aatactacgt ccaaaacgaa cgcttcccgc gactccgcat
     3241 gaaaccacgg aaaggccgcg gagccagcgc agctgagttg ctgaacccgc gatctcgtgt
     3301 acgcaaagcc tggctatccc aaatcgatat caccaagtac tacgcgagat tcaatgtgat
     3361 gccggatagc ttcgccgtct atgcagttgc acgccggttt ttcacagaat ccggcatgct
     3421 cagcggctgg acgcctcaat cagactcata cgaactgacc agttgggttc ccggtggaat
     3481 ggaactgggt ccgcctcgtc ccaaaccacc accaaagccg actgacccca acgcatcccc
     3541 gcccaaacca ccaaacgtga tcgactgatg tttcgtcaca ttgaaagtga ggttgaatga
     3601 gactaaccaa ctcgacgtcg ccgctggttt tgccatggag ctggttttgc catggaaccg
     3661 tggcacgcgt tgcccggtag ttctcgaatt gacttcagac gaagttcgag ttgaaagaat
     3721 caccgttaaa tctcgaatac aatcgtgatc tccagatcga aagcactcgc ccgccgggtg
     3781 ctcttccata acacatttta ggtacacaac agtgacaaga tggattcttt gccttggatt
     3841 gcttctaaca ccagcaatcg catcagccga ccaaactaaa accaacttcg tcttcctatt
     3901 ggtcgacgat ctcggctggg gcgacttcgg cagctacggc gccgaattct acgaaacacc
     3961 gaacatcgat aacctggcca gccaagggat gcagttctcc aatgcctacg ccgcatgcac
     4021 ggtttgctca cccagccgag ccgcaatttt gacgggctgc tatccagctc gaacccacct
     4081 gaccgattgg atcgcgggcc acaaccatcc caaagcaaag ctatcggtcc ccgattggac
     4141 gatgaagatg gcccacagtc gcgtcttgct tcccgaggca ctccacgaag ccgggtactc
     4201 cactgcgttc tttggaaaat ggcacctgat gcccatcgga gctcccgatt tcgacgaaca
     4261 ttatcctacc gcccatgggt ttgacttgaa tgttggtggc cgagaatggg gccaacccaa
     4321 aggacctgga aagtactttt cacctttcgg catgccgaac ctggatgacg gcaaaccggg
     4381 tgacttcctg accgacaagc tgaccgatgc cgccgttgat ttcctggaca acaccacccg
     4441 cgatcaaccc ttccttctgt acttctcgta ctacacactg cacggcccaa tcatgtgccc
     4501 gcctgaactg gaagcgaaat acacccagaa agcaaaaacc tttcaaaaca gcaaaaacga
     4561 acacctcaac gccaaacgtg ccggaatggt cgaatgcttg gacaacagcg tcggacggct
     4621 aatgcagaag ctaaatgact taggaatcgc cgacaacacc gtcatcatcc tgactgggga
     4681 caacggtggc gattacgaca agtcgaccgg aggcctccgc gactacaaag gctactcgca
     4741 tgaaggcgcg gtccgcgaac cactgatcgt tcgctggccc ggcaaaacgt ctgctggttc
     4801 cacttgtgac gagacagtaa tcgggaccga tttctatccc accatgcttg aaatggctgg
     4861 gttaccgccg cgagatagcg aacacctcga cggaaaaagc atcgttccgt tgctgaccgg
     4921 cagctccacc gaattggacc gagacaccgt ctattggcac tacccacact atcaccgaac
     4981 caaaccctac ggagcgattc gcgagggcga ctggaagctg atcgagttct ttgaagatgg
     5041 caggctggag ctattcaatc taaaagagga tgaacaggag gccaatgatc ttgccgaaag
     5101 caatcccgaa aaggcacagc gtcttctaaa gaaactgcaa gcgtggcgaa cgtcggtcga
     5161 tgcccaaatg atgacggaca acccagccta tgatccggcg acagccgaca aaaagaagcg
     5221 acgcccgaag caaaagcagc ctgcaaaaag ctgagtaacg cgtcgtcaat gaaactcgac
     5281 ttcgacatat cagagcccgc aaaggaacat accgatgcgg gctctttgat tttgatcgaa
     5341 ccacctgtgt cgccagcacg aagagggcgg caacccccgt tgtcaccggc gatcagtgtc
     5401 acccgcgatc agctcgcgtc gtggagttga atcttgctga ccttaaaagt ccatgcgtgc
     5461 tcacacggca tcttggacgg gttcaacttt ggcatcttga tgatcaagtc ctcaccggat
     5521 tgttcccatt ccagttcgcc aacgcccaac atggataccg ttgccccttc agcggcttgc
     5581 acatgatgaa gtgtcattgt ttcatcgggt aacaatgcac aaatcgcata cagagtctca
     5641 cccttgcgag tgaacaacaa ttccttgcgg gccatcccct cctcagggct cttcatcaat
     5701 ttctcgacat catacttaac acggtagttt ccacgtccca actcttgagc tttcccggtc
     5761 gaccactggc aggtgctgct gtgtgtcgtc gtgccataaa tggcttcgcc gttgacgtcc
     5821 aaccaatctc cgatttcact caatcgctct tccatcaatg gcggaatacg tccatcggcc
     5881 gtcggaccga tgtcgagcaa gaagttacct ccccgcgaca cgatgtcggc caacatcaag
     5941 atcaacgatt cgcttgaatt gtaatcttcg aaacgctcgg tacggctgta gccaaaggag
     6001 tgggccatgc cacggttttc ttcccaggcg ttagtgccat cgggcaaccc agcgccgtac
     6061 tccgttgtgt aatagccacc gtgcttgtgt cggtcaccct tgccccagcg atcattgatc
     6121 acaacctcgt ctttacaagg tgactcatta aacaaccatg tcagcatctc ggctgacttc
     6181 cagtccgccg caggatgatc ccactcacca tccgagaaga tcaccgcggg ttcatacttg
     6241 ttaacgacat ccttgaactg agggatcaag tgatcatcta caaacgagtt cagcttggtt
     6301 ttgtacagcg ggttgtacca ctcatagatc gagaaataga gccccatctt cacatccgtc
     6361 ttgcgcactt cttcggtcaa ctcaccaacc aagtctcgca tgggacccga gttcgtactg
     6421 caccaagggc gaccgaatga cttatccgcc tgttcattgg gccataggca ataaccatca
     6481 tggtgcttac tggtcaggac cacgtacttg gcaccggact cttcaaatgc ttccgcccat
     6541 tgagccggat cgaacagctc acacttgaat tgatcggcaa actcttcgta cttaaaatcc
     6601 ttcccgtagt tcttttcatg gaacgctttc acagccttgt gagaccgccg ctttgggttc
     6661 atcaagtctt cccagtacca ctcagagtac cgtcccactt cggaaaaggc cggtaccgag
     6721 tagggccccc agtggatgaa aatcccgaac ttcgcgtcct tccaccactg cggtgtgtcc
     6781 cggccatcga tggccttcca ggctgctgcc tcgtcggtga tcgccttgcc ttttgttgca
     6841 accgaagcgg tttgctgagc gcagacacaa gtctgcagga ggccagcgag tatgagtgga
     6901 gtgattattt tgacaaacat tttgtgactt aattggagga gttcgtgagc ccgatgaagg
     6961 gcttccaaca catcgaacgt gaaaaggtga aaggaggcaa aagcaggaga aacgaacgac
     7021 aaggaaaggt caggtgatca ccgatacaaa cgcaacatca gttgggtttc tccagcatga
     7081 tcaatgccga aaccgactga ttagcgggca acgcaagccg ccactgggca ccgtcaaacg
     7141 ttgccgctga tgaatgcacc gactcaatcg gcaaccggct tgaaatcgca accgactgat
     7201 caaccttgga gctcaattcc aaacggatcg tgttctcggg aagatcccaa gccaaccgat
     7261 cgatcaccat ggccttccgc gccagcaccc cagatatttc accgcgtgga agttgcgttg
     7321 caggaatccc gggcaacaaa ttgatctcgc ccggtcgcga ataaacgcac atctcggtca
     7381 agatcgcagg caacgctagg attgaatcga gattgtaaat tcgatgatcg ggattatgag
     7441 aactgatgaa actgcgatag atgaacttgc tacgcgcgta gtcctccagc atgttccaaa
     7501 caatctctgg gtcttgcaaa cgagaaccat agaagcccaa ttccatgaaa ccgtgtgccg
     7561 acttattccc ttgccccgac tcaaggcgtt tttcaatcgc acggcgtgcc gcttgataga
     7621 gttcgggagt cgatgggttg atttccaccc caggataaac ggcgtgcaaa tgagagttgt
     7681 gacgatggtt gtagttgtct tcaacgccgg ggcaactcca ctccgcgatc gcaccgtctt
     7741 cgttgatccg atactctggt acctttttca gaaacgcttg tagttccggg atgcgatcct
     7801 gattgatctc cagttctcga taggcatcaa tcagggcatt gacgctacca cgaatgatgg
     7861 cgatctccga cgtcgcattg ggagcccccg gcgtgttagg cgactggttc tcgggcgaga
     7921 tactggggta aatcaagaac ttcccatcac ggccttccat cgggcccaga aaatcctgat
     7981 agaacagaac ggtgttttcc aacaatggaa cgacgcgttc cgccaagaac tcgcgatcgc
     8041 cagtgaacaa gtaatgctgc cagaaatagg cgcccagcca accggtcgcc cccgtccatc
     8101 cctgccaacc agcgatggcc accccgtgtc gccaaccgtg agcgatcgcc cccatgtagc
     8161 cccgggctcc gtacaactgt tgagcgttgc gttcccaatc aggatacagc gtttcaatcc
     8221 agctgaaata gctctccatc gcctcgggta ggttgccaac acttccgttg gaaatggcaa
     8281 ggttcaaatt cgaatcgttg gtgtaatgcc cccaccacat cggcttccag gtgtcgcccc
     8341 agatacccat caacgtgggc ggtagctcgc cgctgctgga aatcagactg taccggccaa
     8401 cggcgaaaat ccattccagg taagccggcg tggggccatt cgcttccgcc tcggcgatga
     8461 tctcctcggt ggtccttccg tttccaaatt caccacccaa acgatagtga acccggtgaa
     8521 acatctcgcc atgtaccttc gcgtgagcgg ccagcaactc cacataggat tcagacaatt
     8581 cgtccaagtc cgcacgcaaa cgatcacgac tgggctggct cgcaatgggc agaaactctg
     8641 ttcgagtcag caccaagact tcgtcaacgt cttggatcaa aatccgatct gactttgcat
     8701 gggatgcccc gcctttgaca atcacacgcg acatcacctg atagccttcg gcctggggca
     8761 acccatgcag atgacggtaa ctggtgtgca agtacatttc gccatcacca tcttctgcgc
     8821 cctcgatgtg ctcaacgacg ggcgccgcaa tgtcttcttc gttccaaccg ggtctcgcag
     8881 cgaaacgtag ttctgcggtc agtgatgcgt tgctcggacg acgcaatcga gtgacgacca
     8941 cgttatccgg ccgcgaaacg aagatatcac gctgccaatc gccacgatcg tcagtccacc
     9001 tgactgaaac ctcgccgcta tcgagctgca aggtacgtga gtagttcttg acctcaccct
     9061 ttttgggaac cgagattttg aaatacaaag cggggtgaac gtagttcaat ccgaaccgcg
     9121 gaccggggcg gatcaatgct tcttcgggaa acatctctgc gttccgctta ccgaactcgt
     9181 tgaaaacgat gctcgccgcg gggccccact tttcctgctt ggctagctca cgggcctgtt
     9241 tgatagcgga aaccatattg ggaacgtccg gcttgactgg ttgagcaggc acccacaatt
     9301 tctcatggtt caagacaatg gtttcttcga gcggctgccc gtggaccatc acgccctgac
     9361 gcccattccc actaatcaac gcatcgcccc aattggtcga cggctgaccg cttgttaaca
     9421 catagtcgcg tgtggcagcc cgatcgccct tcgtttggga tgcttgttct ggtgcaacct
     9481 cagccgttgt cacaccgtcc aacggcgttg gctgatcagc ccatccacta cttccgttgc
     9541 ccgcccaaaa caaaaccagg aagccaataa cgaagaaatt gatacgcggg tgagccatga
     9601 ccaaatacga agccaagtag tgtggtatcc agcgtcgata ccatgcaaga agttagcgtg
     9661 agacagtgta aatactgccg catgacacca aaccagcctt gccaactcat gcgaaactac
     9721 gtttttctac ctcgatgcct gggttctacc accccggcaa cgaggcaaga caaactgcgt
     9781 ttcacctttg acaacaaaca accgccacga cgtcagccta ggaagctggc ttgcccgcgt
     9841 tgtaaagctg ctcgatttcg gccggggaca aggcacgatc gaataccgca aactcatcaa
     9901 tttgcccgtt gagatttcgg attggccact catcgtaatg acgcttggga ctccagttcc
     9961 ccagctccgc tttgccaatt cggaacttgt actctccctt tggcaactcg ccgtcataat
    10021 acgggctcat tccaataagt tccccgttga cgtagtgccg cacctccgtt gtttgcgaat
    10081 cggcaacggc agcgatatgg aaccaacgcc ccaagtcatc gtacccaaac accggctttg
    10141 aaacacaaat gacgcgatcc tcacctggcg gcttgatacc ggtatcaata gaacccgatg
    10201 aacggatctg ccagtgaatg tcgccgtttt cataagcatc ggtcagcatg atggaattga
    10261 aatgccgatc aaagccatcg attcgcatcc aagcactcat tgtcaatcgt tcgaattcgc
    10321 ccgggacctc aaaacgcact cggtcgtcag ggcgtttgaa ctccaaagcg ttcttgccct
    10381 tccatcgacc ttgtgtccag cggcaaccaa tgaccgagcc atcgactgaa tcactgcaaa
    10441 ggttgttcag ttcatacgga tgatcgtctt tgtgctgaaa gtcgtaaaag accagcaaac
    10501 tcgggtcgct ggacaattca tcgcgatacg cccgccattc ctgatgccgc ttgacttcag
    10561 acgactcctt cagcaaccgc aactcattgc tgctaggaaa ctgctgagcg tcggaatcaa
    10621 ttcgttgctg ccgatcctcc acctgcttga ggccattgcc tgatgtgatc cgctgcaccg
    10681 ggacatctcc acgactactg cggacctcaa cctccccatc aaacacatgc agttcttgac
    10741 gaccgtcacg cgttgtttcg agtgcgaagt cagtaccgag gtcaacgtac ttggtttctt
    10801 tggactggat cgtgaatccg ctagccgaag gcggcacgtg acagcgaact ttcccataga
    10861 acaacaaccc tgacatccgg gacaccaatt caatctttgc tggcccttcg atgatagcgg
    10921 tcgcaccgct taagaaacct agctcaacca ccccctcgga tagttcaatg gtttgcggac
    10981 caagcatatc accaatttga atgccccggt ttggcgtcac ccatttcgca gttcccgaat
    11041 ctcgcaacac tgcaacattg agattggcaa cagcacgatc aaaccgcggt gcattcacca
    11101 cggtatgagc actcgttcga gtaatcttct gggatcccga agctgcatct tcctcgtaat
    11161 agaccccttc gcggtcagcg tccgaactgg gctgccaggc gacgtcacgc ggagcgcgag
    11221 ccagaaaagt ctgagccccc aacgcgaata gcaccaccgc agcacaagcg aacaagaccc
    11281 aacccacgcc ggaagcgttg ctgtaaacga tcccctcttc ctgcgagtca acgcccagcg
    11341 gaaccagctt cggtggtgac atcggcttgg acgcatccac ctcgggcgca ttgcctacca
    11401 attcagagac attgtcatgg gaagcgtcgg tcagttcgtg caaagctgcg tgcagcctca
    11461 acacacgacg aaactccttc cgggcctctg ggctctcacg gagtgtatcc tgcaacaggt
    11521 caaactgatc tgcatcgatt acgttggaac agtagcgttg gactaattct tcgaaattgg
    11581 cggagatcat tcgttgccca atgctggggt aacaggctgg cacttgtggt cgatacaacg
    11641 ttgtaggcga cggcgaagtc ttgcgacggt cttgtaaaga ctgttggccg tcacatccaa
    11701 tcggtctgcg accgacgtca gcgattggtt cgtcccgtat gccgtgacaa tcaactcccg
    11761 ctcagaatca tcgagcttgg tcatacaatc gtccaagtag tgcagcattt ggtcatcggc
    11821 ctgaaagtct tcttcgctgg cgtattcaga agccaactga gcgagtagat catcatcaaa
    11881 aacaaatcga ttgcgtgcca gtttgcggcg gtgcatttga gcttcatacc gagccaccac
    11941 gtaggaccac ttcaaaaagc cgtcgtcgtc ttgcaaagct gaaaactttt cccacagcac
    12001 gacactgacg ttttgcatta cttcgtcaac cgcatctcgg ttcacaagca atccgtacaa
    12061 aaacgcccga atccgccgtt cgttggcagt aaaaagacgc acgaatctcg cgacgtcgcc
    12121 ccccgctcct gcctgattat ccac
//
