LOCUS       NZ_CP102288            15235 bp    DNA     linear   CON 29-JUL-2024
DEFINITION  Segatella copri DSM 18205 chromosome, complete genome.
ACCESSION   NZ_CP102288 REGION: 3094181..3109415
VERSION     NZ_CP102288.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20222705
            Assembly: GCF_025151535.1
KEYWORDS    RefSeq.
SOURCE      Segatella copri DSM 18205
  ORGANISM  Segatella copri DSM 18205
            Bacteria; Pseudomonadati; Bacteroidota; Bacteroidia; Bacteroidales;
            Prevotellaceae; Segatella.
REFERENCE   1  (bases 1 to 15235)
  AUTHORS   Cheng,A.G., Ho,P.Y., Aranda-Diaz,A., Jain,S., Yu,F.B., Meng,X.,
            Wang,M., Iakiviak,M., Nagashima,K., Zhao,A., Murugkar,P., Patil,A.,
            Atabakhsh,K., Weakley,A., Yan,J., Brumbaugh,A.R., Higginbottom,S.,
            Dimas,A., Shiver,A.L., Deutschbauer,A., Neff,N., Sonnenburg,J.L.,
            Huang,K.C. and Fischbach,M.A.
  TITLE     Design, construction, and in vivo augmentation of a complex gut
            microbiome
  JOURNAL   Cell 185 (19), 3617-3636 (2022)
   PUBMED   36070752
REFERENCE   2  (bases 1 to 15235)
  AUTHORS   Cheng,A.G., Jain,S., Meng,X. and Fischbach,M.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUL-2022) Chem-H, Stanford University, 290 Jane
            Stanford Way, Stanford, CA 94305, USA
COMMENT     REFSEQ INFORMATION: The reference sequence is identical to
            CP102288.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2021
            Assembly Method        :: Unicycler v. 0.4.8
            Assembly Name          :: Prevotella-copri-DSM-18205-MAF-2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 100x
            Sequencing Technology  :: Illumina HiSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_025151535.1-RS_2024_07_29
            Annotation Date                   :: 07/29/2024 03:59:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,718
            Genes (coding)                    :: 2,663
            CDSs (with protein)               :: 2,663
            Genes (RNA)                       :: 78
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 13 of 55
            Pseudo Genes (incomplete)         :: 44 of 55
            Pseudo Genes (internal stop)      :: 9 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..15235
                     /organism="Segatella copri DSM 18205"
                     /mol_type="genomic DNA"
                     /strain="DSM 18205"
                     /host="Homo sapiens"
                     /culture_collection="DSM:18205"
                     /type_material="type strain of Prevotella copri"
                     /db_xref="taxon:537011"
     gene            1..2325
                     /locus_tag="NQ544_RS12960"
                     /old_locus_tag="NQ544_12960"
     CDS             1..2325
                     /locus_tag="NQ544_RS12960"
                     /old_locus_tag="NQ544_12960"
                     /inference="COORDINATES: protein motif:HMM:NF012754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="WP_006847602.1"
                     /translation="MKKMVFTLALLLMSLSAALAQTSTFKITHAVARNSKVNQMYVTT
                     KSGDVKYYNTADLTSVKFEGDKAIITPKSGSENDEYNASVQAIRFAKKADQGESGDID
                     NPAGVIQITEAKGWQESAYLKWAPFEGASSYNVYVDDKKIDAQLVRQYKSYYRADVLG
                     LKEGTYSVKVVPVNADGKEIAGANTASNLVVKSYNREGFAHFKYDGVGAYNNDGTLKA
                     GAKVLYITAKTAKTVSTTVNTGKLETITGLQSIIDAYSKGKDTTPIAFRIIGKVNLSD
                     LDHISSSAEGLQIKGAMMNMTFEGVGDDATVYGFGFLLREAESVEFRNFAIMRCLDDA
                     MSLDTNNSHVWIHNMDLFYGKKGSAADQAKGDGTVDIKGDSKYVTVAYNRFWDNGKAS
                     MCGMKSETGENWITYHHNWFDHSDSRMARVRTMSVHMYNNYYQHCDVYGIGATSGSSI
                     FMESNYFDAVKRPIMSSLQGTDAKGDGTFSGEKGGLIKAYGNVFTNKPANFSYIPYAE
                     NNTSFDAYEVSHPSEQVPASVKTLVGGTSYDNFDTNPSLMYAYAADKAEDVPSIVEGF
                     YGAGRLNHGDIDFVIPDETVVTNGHQQPWPALASILDAYTSGVVKVFGESNASGEGGS
                     AEGGSTGGSGEGGSTGGTEGGSIITPIEGTVLVTFTDSKPSSSIVTVSGNYATNKGTA
                     TIDGTSYSTCVKMESATNISVTVDKKVTMTLYFSSADTKTNAKIDGKKPAEVNAVIDS
                     TAKTMTVTLDAGSHTITKQDTCNLFGIKLVPVTE"
     gene            2526..5492
                     /locus_tag="NQ544_RS12965"
                     /old_locus_tag="NQ544_12965"
     CDS             2526..5492
                     /locus_tag="NQ544_RS12965"
                     /old_locus_tag="NQ544_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005680364.1"
                     /GO_component="GO:0009279 - cell outer membrane [Evidence
                     IEA]; GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="WP_006847601.1"
                     /translation="MNAIQNLAKRSLLLVALFVIGCLQLMAQTRTIKGEVTDAQNGEA
                     LIGATVMVEGEKGGTVTDFDGNFSLQVSSSAKKIKVSYIGYIDKVLSVSDNMKVKLES
                     DSKALADVVVIGYGTARKSDLTGSVATVKSKDFNKGLVSSPEQLINGKVSGVQIMSNS
                     GSASAGSTIRVRGGASLNASNDPLIVLDGVPLEQGGISGNSSNFLSMINPSDIESMTV
                     LKDASSTAIYGSRASNGVIIITTKKGQQGAVKVNFNTTNSLQTRAQMVDMLSRDEFVN
                     VINQFGDANQKSLLGTANTDWNDVVYRTAFGTDNNLSVSGSIDKWLPFRVSVGYYNQS
                     GLVRKDNVERWTGNVVLTPSFFQDHLKLTINAKGTLNNNSFNNGGAVWAAATFNPTIP
                     VYSGNDKYGGYNEALDADGVPVNAGVRNPRGLVDLYDSKSKVSRFIGSMDVDYKVHFL
                     PDLKLHATVGADYAKGDGTIYVPAYAAQSYNKDESLGGSDYKYGPQKNENRLLTLYAN
                     YAKYFEDIKSNVDLTAGYDYQYWKSTTPLYYTKSAAGTNLSTVKASDYRHVMLSYYGR
                     INYSFDGKYLLTATVRRDASSRFSKDTRWGTFPSVALGWTLTEEPWLKNQKVLSNLKL
                     RASYGVTGQQEGIGNYNYLPVYTYSVTGAEAFINGQYINTYRPEAYVSDLKWETTTSW
                     NFGLDFGFLDGRIGGAIDFYTRKTKDLLASVPTAAGTNFSKTILTNVGNVDSKGIEVS
                     LNATPIQTKDWEWNLSYNFTWQDMKVKNLSLTKGGSQTNVKVGPSIDAYQFQVLSEGY
                     EPYMFYVYHQLYDSKTGKPIEGAYADLNNDGEINESDLYRYHSPAPKYIMGLSTSLRY
                     KQLTLGMSFRANIDNYVYNGMGMSTGAFETVSYNNSQLNNLNTSFLKTGFKTRQYLSD
                     YYVENASFLKLDNLSLSYNVGKINKWASLTVSAMVQNVFTITGYSGTDPEVPNGMDNS
                     FYPRPRTYSVSLGLQF"
     gene            5538..7223
                     /locus_tag="NQ544_RS12970"
                     /old_locus_tag="NQ544_12970"
     CDS             5538..7223
                     /locus_tag="NQ544_RS12970"
                     /old_locus_tag="NQ544_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007214785.1"
                     /GO_component="GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="WP_040553102.1"
                     /translation="MMMGTAVLTGTTSCVSDLDQYPHTETTSKDVYTSLANYEAVLGK
                     IYAAMVTSGQGKGGDNKDMESVLNKGAGFDYMRMFINMQECGTDEFASTWLTGEQTTG
                     LTYLSWDANDAWVSDMYYRIYYNIALCNEFLRNANSANFSGADAEKMKEYKAEVRFMR
                     ALFYYHALDFYRNIPMVTENDPVGSYIPPRYTPQQTFDYIESELKDCVGDMLPASTCP
                     YGQASQGAAYTLLAKLYLNSEVYTGVDKYAECKEACEKVMDMGYSLESDYSKLFNADN
                     DKRTNEIIFALPVSAEHIVSWGSSTYLVCGQVSLSNANQNVADYGVTAGWSEFRLRPE
                     FVDKFTQTDIDGGDDGDKRCKFFTNGQSKDISSMTTETAGYLSEKWSNLKDDGITAAS
                     NTADAGVETDFPLFRLADVYLMYAECVVRCKDANDKPLDWDPWNGGININDQTVIESR
                     KKGAIYWINLLRERAYGKDENGNPRGQVWKENFSSKEAFLQFILDERARELYHEGYRR
                     TDLIRYGQFTTNKYIWQWKGGTHDGQAVDSKYNIYPIPNTELTANPNLHNDNY"
     gene            7236..8645
                     /locus_tag="NQ544_RS12975"
                     /old_locus_tag="NQ544_12975"
     CDS             7236..8645
                     /locus_tag="NQ544_RS12975"
                     /old_locus_tag="NQ544_12975"
                     /inference="COORDINATES: protein motif:HMM:NF028448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5114 domain-containing protein"
                     /protein_id="WP_006847599.1"
                     /translation="MKKIFRNLMMLLCALTALVSCDESGDKIYLDGFKASDLMASASD
                     VKLSVDNSKDVVLSLAWQNPTLLSSDETKPAGSGVLKTYLQVSASENFTSEKEYTVTD
                     LSKAFTGADLNAAAKDLGLSPDVSSPLYFRIKSQMGANLDAAYSNVCQVKVTPYLIDM
                     SYINILNENKEQVLTKLYSPNSDGVYSGYMNASSWFHIWGKENDGTIWGNVGQDGHVY
                     EMDNTESAWNIWFPGQTGIYYTVLDTKAKELKPTYIKSMQLNGEDMTYDAPNYAWTKV
                     ITTTADNTPVSIVATGAEYSKATGTEDAAAVEKTLNYTLADGKMTDSENAGSVNIPTA
                     GTYTITVKVGDKSDLTYSIVSGDQTTPKPTISNTIGMFSKDGSTLYATFTKVSEGVYT
                     CTYDLSNLYDMRFYFIGDDIDDKRVCYGSVPNSQFSLYKEEDDSNRQGWDIWFNDDAK
                     SMESATITVDLNTMTWKYE"
     gene            8664..9758
                     /locus_tag="NQ544_RS12980"
                     /old_locus_tag="NQ544_12980"
     CDS             8664..9758
                     /locus_tag="NQ544_RS12980"
                     /old_locus_tag="NQ544_12980"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007901231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 53 protein"
                     /protein_id="WP_006847598.1"
                     /translation="MKNIFGKAILLASALLFSITGTSCSNDDNTTSEKEKIYDMSGFA
                     KGADVSWLTEMEKDGVKFYNQNGKATECMKLLREEGTNSIRLRVWVNPEGGWCGKDDV
                     IAKAWRAQQLGFRLMIDFHYSDTWADPGNQKVPAAWQGYTAEQMKQAVADHTKDVLKA
                     LKDRGVTNVEWVQVGNETRDGMLWNSDEAVTGQVSKNAANFAAYINVGYDAVKEVYPN
                     AKVIVHVDQGQDLGGLTWLYDKLKENGGKWDVIGLSLYPEDDNWQSYAESCLSNIQTL
                     SSMYGKDVIISEIGMWWGSDQAAPLMKKMVDGCKKISTCEGVFYWEPEVYNNWKPANY
                     ITLGWSAYTKGAFDNSGKPTAVFDAYKNIK"
     gene            9763..12213
                     /locus_tag="NQ544_RS12985"
                     /old_locus_tag="NQ544_12985"
     CDS             9763..12213
                     /locus_tag="NQ544_RS12985"
                     /old_locus_tag="NQ544_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013063425.1"
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="WP_228023633.1"
                     /translation="MIKRSITFMTLALALTTLCQAQSRKIINLPSWDFSRDGKAWQQV
                     AVPHDWAISGPFDKKWDLQMVAIEQNGETEKTEKSGRSGALPWIGEGMYKMNWTAPKG
                     YKRAVLVFDGAMSQPVVSVNGKEAGRWAYGYNAFRIDITPFIQFGKSNLIEVHLNNVE
                     ESSRWYPGGGLYRPVSVELYGNENFSTWDTFVRTLKADKQEAEVEVNALLEGKTGKSG
                     KTVIALLDEAGKKVAEQIVTGANPEIKTTLKVANPQLWSPESPYLYQVKLTRYEGKKV
                     ADVQTLKTGIRTIAVSKNNGFQLNGITRKLKGVCLHHDLGPLGAAENKAALIRQIKMM
                     KQMGCDAIRTAHNMPSTMQMELCDSLGMMVMAESFDMWIYPKCKNGYAKFFKEWSDKD
                     ITNLVKHHRNHPSIVMWSIGNEIPEQWSKEGMEIAKHLQDICHQYDPSRPVTQGMDKA
                     EDALKSGFAQVMDVPGFNYRVHKYFKNIEQLPQGFLLGSETASTVSSRGVYKFPVVVS
                     DKATYPDGQCSSYDTEYCSWSNLPDDDWKMQDDYSWVIGEFVWTGYDYLGEPTPYDTY
                     WPSRSSYFGICDLAGLPKDRYYMYRARWNEQQHTTHLLPHWNWKGREGQVTPVYCYTD
                     GVEGELFVNGKSQGRVRKDKSSRLDRYRLRWNNVKYEPGEIRVVTYNQYGDKIGEDVK
                     RTAGEPAQMKFSVETPDHEPIACMVEGCTDEHNVLLNADGNDLAFITVSLQDKDGNEC
                     PLADDELTFEVSGAGTFKAACNGDATSLEPFTQPQMKLFSGKLVVIVQSSKQKGDIIL
                     KVKDSKRNIEQSITLQAI"
     gene            12356..15235
                     /locus_tag="NQ544_RS12990"
                     /old_locus_tag="NQ544_12990"
     CDS             12356..15235
                     /locus_tag="NQ544_RS12990"
                     /old_locus_tag="NQ544_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="WP_006847595.1"
                     /translation="MKKSKEPISRISMAIAMLFVSLAIHAQRFVNTSLEGAQTVCAIT
                     QNSDGMIWLGTDNGLYSYDGYHGYRHFQEHTFSNTRVNALGFEGRMLYLATGNGILKF
                     DTQSYQYAETPAMKAFADKAKRKQLKELRVLDIKGGKTDFGGDVYALLSTPRGLLVGS
                     LAGLHLGSRLIPLRAGEQPLVNALAYDAKRRCYWIGTEGALYCADLQLRNFSQIPALN
                     GNSIKCLSTDAAGNLYIGTDNGLYQMALSNAITHYIHDSRDDTSIPNNIVWACFVDKW
                     QNVWVGTDNGLSRLTTHTYYRYVSLDKITMSGEGNCLHAMLQTRNGEWWMGGTNGLIH
                     FTRNAEGYQNVAWYKQNSSAFPLSHNRVRKIYEDHDGDVWICTDHGINFYDRSSRQMR
                     NFIVYDKSGKYSTAWAYDILQDKKGRIWMGSYQGGVFVMDKAALLSGKTIADWHYSDK
                     GKNALSGLHVGQMVMDGKGRIWVSTYTRLDCINPNDMKVVQVANSDVVNYLMADSKGN
                     IWMGDNNSVTCYYVAGTVLGNSFSSKTWQIGGKVSTMCDVEGKIWVVSGNECCVINPE
                     GESQRFMIPSVVPLNICYSRQTHEVMMGGNDGYVAISSDVAQKPKLFTRLMLAGIIVN
                     GQACEERGEILKLKSDENNFTLQLTDLPFADHPSAVYAYKLEGSDHDWHYLNSGNIDI
                     TYNGLSYGDYHLTVHAVDGEGKIAAEVYSLDISVLPPWYLSFWCKLFYITALIVFVAW
                     LVSWYFLRKELADAQKQKAEVLEQVQMRIDFFNRLTEDLKAAVAHRSFDEVTALMVRY
                     LNVKMPEEPSAVATGLSASPESEPESSSSEPVPARSESSEEEKKEVDVSELDVSDKRL
                     LEEINEAIEKHMIDSDFNVSMLQDVIGIGGKQLYRKMKAITGRTPVEYIREMRMNRAA
                     ELLSQGKFSVSEVMYTVGFSNSSYFSKCFSKEYGMTPTEYMKVKR"
ORIGIN      
        1 atgaagaaaa tggtatttac tttggcactc ctcttgatga gtttgagtgc agctttggca
       61 cagacttcga catttaagat tacccatgcc gtggctcgta acagcaaggt gaaccagatg
      121 tatgttacca ccaagtcggg tgatgtaaag tattataata ctgcagactt gacaagtgtg
      181 aagttcgaag gtgataaggc aatcatcaca cctaaatcag gttcagagaa tgatgagtac
      241 aatgcttcag ttcaagccat cagatttgct aagaaggccg atcagggcga aagtggtgat
      301 attgacaatc ctgcaggtgt gattcagatt actgaggcaa agggctggca ggagtctgcc
      361 tacctgaagt gggctccatt cgagggtgct tcttcttata atgtatatgt agatgacaag
      421 aagattgatg ctcaactggt tcgtcagtat aaatcttact atcgtgctga tgttctcggt
      481 ttgaaggaag gcacttattc tgtaaaggtt gttcctgtaa atgctgacgg taaagagatt
      541 gctggtgcca atactgcttc taacttggtt gtaaagagct acaaccgtga gggctttgct
      601 catttcaagt atgatggtgt aggtgcttat aataatgatg gtactttgaa ggctggtgct
      661 aaggttctgt atattacagc caagacagct aagaccgttt ctacaaccgt taatacgggt
      721 aagcttgaga cgattacagg tcttcagtct attattgatg cttattctaa gggcaaggat
      781 actactccta tcgccttccg tatcatcggt aaggtaaatc tttctgattt ggatcacatt
      841 tcaagttctg ctgaaggatt gcagattaag ggagctatga tgaatatgac attcgaaggt
      901 gtcggtgatg atgctaccgt atatggcttc ggcttcttgc ttagagaggc tgagagtgta
      961 gagttccgta actttgctat catgcgttgt ctggatgatg ccatgtctct tgataccaat
     1021 aactctcatg tatggattca caacatggat ctcttctatg gtaaaaaggg tagtgctgcc
     1081 gaccaggcta agggtgacgg tactgtagac atcaagggcg attctaagta tgttactgta
     1141 gcttacaacc gcttctggga taatggtaag gcttctatgt gtggtatgaa gagtgaaact
     1201 ggtgaaaact ggattactta tcatcacaac tggttcgacc attcagactc tcgtatggct
     1261 cgcgttcgta ccatgagtgt tcacatgtac aacaactact atcagcattg tgatgtttat
     1321 ggtattggtg caaccagtgg ttcaagtata ttcatggagt ctaactactt tgatgctgtt
     1381 aagcgtccta tcatgagctc tttgcagggt actgatgcca agggtgatgg tactttctct
     1441 ggtgagaaag gtggtttgat taaggcatac ggcaatgtat ttaccaacaa gccagcaaac
     1501 ttcagctata tcccatacgc agaaaacaat acaagttttg atgcctacga ggtttctcat
     1561 ccaagtgagc aggttcctgc aagcgtaaag actttggttg gtggtacttc ttacgacaat
     1621 ttcgatacga acccaagcct catgtatgca tacgctgcag ataaggcaga agatgttcct
     1681 tctatcgtag aaggtttcta tggtgcaggt cgtttgaatc atggtgacat tgatttcgtt
     1741 attcctgatg agacggtagt aaccaatggc caccagcagc catggcctgc tttggcaagt
     1801 attcttgacg cttatacttc tggtgttgtt aaggtattcg gtgagagcaa tgcttctggc
     1861 gaaggtggtt ctgctgaagg tggcagcact ggtggttctg gcgaaggtgg tagcactggt
     1921 ggtacagaag gtggcagtat tataactcct atcgagggta ctgttttggt aacattcaca
     1981 gattctaagc cttcaagtag catagtaaca gtttcaggta actatgctac aaataaggga
     2041 actgctacta ttgacggcac ttcatattct acttgtgtaa agatggagtc tgcaactaat
     2101 atctcagtaa cggttgataa gaaagtcaca atgacccttt atttcagttc tgcagatacc
     2161 aagacgaacg ccaaaattga tggaaagaag cctgctgaag taaatgcagt tattgattca
     2221 acagctaaga caatgactgt aacattggat gcaggttctc atactattac aaagcaggat
     2281 acatgcaacc tcttcggtat taaacttgta cctgtaacag agtaaaggta tagaactgat
     2341 ttaaagattc ctgcctcttt aatgaaaaaa cggacaaaag ctagataaat atttgctttt
     2401 gtccgttttt tctttatttt tgtcttaaat ctatctgctg ttttcttctt ttttctctac
     2461 ttttgcaact gaaaacaatt ttaaacgtaa tatttaaacg taaaacgcaa cctaaaagta
     2521 aaacaatgaa cgcaatccag aatttagcaa agagaagttt gcttctcgtg gcattgttcg
     2581 tcatcggatg tctgcaactc atggcgcaga caagaaccat caaaggtgag gtgactgatg
     2641 cgcagaatgg tgaggctctg attggcgcca ctgtcatggt ggaaggcgaa aagggcggta
     2701 cagttactga ttttgacggt aacttcagtc ttcaggtatc ttcttcagcc aagaagatca
     2761 aggtatctta tatcggttat attgataagg tactttctgt ctcagacaat atgaaggtga
     2821 aactcgaatc tgacagcaag gcgctggctg atgtcgtggt catcggatat ggtacggcaa
     2881 gaaagagtga cctgactggt tccgtagcta ccgtgaaatc gaaggacttt aataaaggtc
     2941 tcgtttcttc tccagaacag ttgatcaacg gtaaggtttc gggtgtacag attatgtcca
     3001 acagtggttc tgcctctgcc ggtagtacca tccgtgtgcg tggtggtgca tctcttaatg
     3061 ccagcaacga ccctcttatc gtgctcgatg gtgtgccatt ggagcagggg ggtatctcgg
     3121 gtaacagcag caacttcctc agcatgatca acccttctga tatcgagagc atgactgtat
     3181 tgaaggatgc ttcttctaca gccatctatg gttcccgtgc atctaacggt gtcatcatta
     3241 ttactaccaa gaagggacag cagggtgctg taaaggtgaa cttcaatacc accaacagtt
     3301 tgcagacccg tgcgcagatg gtagatatgc tcagccgtga tgaatttgtg aatgtcatca
     3361 accagtttgg tgacgctaat cagaaatcat tgctcggtac tgccaatacc gactggaatg
     3421 atgtggttta ccgtactgcc ttcggtacag ataataacct cagcgtgagc ggcagcatcg
     3481 acaagtggtt gccattccgc gtgtctgtag gctattacaa ccagagcggt ctggttcgca
     3541 aggacaatgt agagcgttgg acaggtaacg tggtattgac tcctagcttc ttccaggatc
     3601 acctgaagtt gaccatcaat gccaagggaa cgctcaataa caactcattc aataatggtg
     3661 gtgccgtatg ggcagctgcc acattcaacc caaccatccc ggtttattcc ggtaacgaca
     3721 agtatggtgg ctataatgag gcactcgatg ctgatggcgt tccagtgaat gccggtgtaa
     3781 gaaaccctcg cggtctggtt gatctgtatg attcaaagag taaggtaagc cgcttcatcg
     3841 gttctatgga tgtagattac aaggttcatt tcctgccaga cctcaagctc catgctaccg
     3901 tgggtgctga ctatgccaag ggcgacggaa ccatctatgt tcctgcctac gcagcacaga
     3961 gctacaacaa ggatgagtct ttgggcggta gcgattacaa gtatggtcct cagaagaacg
     4021 agaaccgctt gcttaccctc tatgccaact atgcaaagta tttcgaagat atcaagagta
     4081 atgtagatct gacagccggt tacgactacc agtactggaa gagtactaca cctctttatt
     4141 ataccaaaag tgcggcaggc acaaacctgt caaccgtaaa ggcatcagat taccgtcatg
     4201 taatgttgtc atattacgga cgtatcaact attcattcga tggaaagtat ctcctgacgg
     4261 caaccgttcg tcgagacgca tcttcccgtt tctctaagga tacccgttgg ggtactttcc
     4321 catctgttgc tttgggctgg actttgacag aagagccttg gttgaagaac cagaaggtac
     4381 tttctaactt gaaactccgt gccagctatg gtgttaccgg tcagcaggaa ggtatcggca
     4441 actacaacta tctgccggtt tatacctaca gcgtgacagg tgccgaagca ttcatcaacg
     4501 gacagtatat caatacttac cgtcctgagg catacgtatc agacttaaag tgggagacta
     4561 caacctcctg gaacttcggt ctcgatttcg gattcctgga tggtcgtatc ggtggtgcca
     4621 tcgatttcta tacccgtaag accaaggacc tgttggcatc tgtaccaaca gcagctggta
     4681 ccaacttctc aaagactatc cttaccaacg taggtaatgt ggacagcaag ggtattgagg
     4741 tttctctgaa tgcaacccct atccaaacca aggactggga gtggaatctg agctacaact
     4801 tcacctggca ggacatgaag gtgaagaacc tctcactcac aaagggtgga agccagacca
     4861 acgtgaaggt aggtccatct atcgatgctt accagtttca ggtactctca gagggatatg
     4921 agccatacat gttctatgta tatcatcagc tctacgactc taagacaggc aagccaatcg
     4981 agggtgctta tgctgatctc aacaacgatg gcgaaatcaa tgagtctgat ctctaccgct
     5041 atcattctcc agcccctaag tacatcatgg gattgagtac atcattgaga tacaagcagc
     5101 tgacactcgg tatgagtttc cgtgccaaca tcgacaacta tgtatacaac ggtatgggta
     5161 tgagtacagg tgcttttgag acagtaagct acaacaactc acagctcaat aatctcaaca
     5221 ccagtttcct gaagacaggt ttcaagaccc gtcagtatct ctcagattac tatgtagaga
     5281 atgcttcgtt cctgaaactc gacaacctga gtttgagtta caacgtaggt aagatcaaca
     5341 agtgggcttc acttaccgta tctgcgatgg tacagaatgt attcaccatc accggttact
     5401 caggtacaga ccctgaggtg ccaaacggaa tggacaactc attctatcca cgtcctcgca
     5461 cttactcagt aagcctcgga cttcagttct aacaatcaaa acagataaca atgaaactta
     5521 agaatatatt atatagtatg atgatgggaa cagcggtgct gacaggcaca accagctgtg
     5581 tttccgatct cgaccaatat cctcatacag agacaacctc taaggatgtt tatacctcgc
     5641 ttgccaatta tgaggctgtg ctgggtaaga tttatgctgc catggtgacc agtggtcagg
     5701 gcaagggcgg cgataataag gatatggaat ccgtattgaa caaaggagca ggtttcgact
     5761 acatgcgtat gtttatcaat atgcaggagt gtggtaccga tgaatttgcc tctacctggc
     5821 tgactggtga gcagactacg gggcttactt atctttcctg ggatgccaac gatgcgtggg
     5881 tatcagatat gtattatcgt atctactaca acatcgccct ctgcaacgag ttcctgcgca
     5941 atgccaacag tgccaatttc tcgggtgctg atgctgagaa gatgaaggag tataaggcag
     6001 aagtccgttt tatgcgcgct ctgttctatt accatgccct cgatttctat cgcaacattc
     6061 cgatggtgac agagaatgac cctgttggca gctacattcc tcctcgttat actcctcagc
     6121 agacattcga ttatattgag agcgagttga aggattgtgt aggtgatatg cttcctgctt
     6181 ctacctgtcc ttacggtcag gcttctcagg gtgctgccta taccttgctt gccaagcttt
     6241 atctgaacag cgaggtttat actggcgttg acaaatatgc tgagtgcaag gaggcttgtg
     6301 agaaggtgat ggatatgggc tactctttgg agtctgatta cagcaagctc ttcaatgctg
     6361 ataacgacaa acgtaccaac gagattatct tcgccttgcc ggtaagtgct gagcatattg
     6421 taagctgggg ttcttctacc tatctggttt gcggacaggt gagtctgtcg aatgctaacc
     6481 agaatgtagc cgattatggt gtaactgccg gttggagtga attccgtctt cgtcctgagt
     6541 tcgtggataa gtttacccag accgatattg atggcggcga cgacggtgac aagcgttgca
     6601 agttcttcac caatggtcag agcaaggata tttccagcat gactacagaa accgccggtt
     6661 atctttctga gaagtggagc aacctgaagg atgatggcat tactgcagca tctaatactg
     6721 ccgatgcagg tgtagaaacc gatttccctc tcttccgttt ggcagatgtc tatctgatgt
     6781 atgccgagtg cgtggttcgt tgtaaagatg ctaatgataa accactggat tgggatcctt
     6841 ggaatggagg tatcaatatt aacgatcaaa ctgtaattga aagccgtaag aagggcgcta
     6901 tctattggat taaccttctc cgcgagcgtg catatggaaa agatgaaaat ggaaatccta
     6961 gaggacaagt atggaaagag aacttctcat caaaagaagc cttcctccag ttcatcctcg
     7021 atgagcgtgc ccgtgagctt tatcatgaag gttatcgccg taccgacctg attcgctatg
     7081 gtcagttcac aaccaacaag tacatctggc agtggaaggg tggaactcac gacggacagg
     7141 cagtggatag caagtacaac atctatccaa tccctaacac cgagcttacc gccaatccaa
     7201 atcttcataa cgacaactat taataggata aaagaatgaa aaagatattt agaaacctga
     7261 tgatgcttct ctgcgcactc accgccctgg tgagttgcga cgagagtggc gacaagatat
     7321 atctggatgg cttcaaggca tccgatctga tggcatcggc atccgatgtg aagctgtctg
     7381 tagataacag taaggacgtt gtcctctcgt tagcttggca gaatcctacc ttgctttcga
     7441 gcgatgaaac caagcctgcg ggcagtggag tgctgaaaac ttatctgcag gtatcagcct
     7501 cagagaactt tacttcagaa aaggaataca ccgtaaccga cctttccaag gctttcaccg
     7561 gtgcagacct caatgctgca gccaaggatc tcggcttgtc tcctgatgtg agttcacctc
     7621 tttatttccg tatcaagagt cagatgggtg ccaatcttga tgccgcttac agcaatgtct
     7681 gccaggtaaa ggttactcca tacctcattg atatgagcta catcaatatc ctgaatgaga
     7741 ataaggaaca agttctgaca aaactctatt ctcctaattc tgatggagtt tattctggct
     7801 atatgaatgc ctcttcttgg ttccatatct ggggtaagga aaatgatggt accatttggg
     7861 gtaatgtagg acaagatggt catgtgtacg aaatggataa tactgagtct gcatggaaca
     7921 tctggttccc aggtcagaca ggtatttatt atacagttct tgatacaaaa gctaaggaac
     7981 ttaagccaac ttacatcaag tcgatgcagt tgaatggtga agatatgacc tatgatgctc
     8041 ctaactatgc ttggaccaag gtgattacaa ccacagccga taatactcca gtcagcattg
     8101 tagctacagg tgcagagtac agcaaggcta ctggtacaga agatgctgca gccgttgaga
     8161 agacattgaa ctatactttg gctgatggca agatgacaga ttcagagaac gctggtagcg
     8221 tgaatatacc tacggcgggt acttatacca taaccgtgaa ggttggagat aagagtgatt
     8281 tgacttattc tatcgtatcg ggagatcaga caactccgaa accaaccatc agtaatacta
     8341 tcggtatgtt ctcgaaagat ggcagtactt tgtatgcaac ctttaccaag gtgagtgaag
     8401 gcgtatatac atgtacctac gatttgtcga atctatatga tatgcgtttc tattttatag
     8461 gagatgatat agatgacaaa cgagtatgtt atggttctgt tcctaacagc caattctctc
     8521 tttataagga ggaagatgat agcaatcgcc aaggttggga catctggttt aatgatgatg
     8581 ctaaaagcat ggaatcggca acgattacag ttgacctcaa caccatgaca tggaagtatg
     8641 aataattaat aagaaaaata gcaatgaaga atatatttgg aaaagccata ttgctggctt
     8701 cagcactctt gttttctatc acgggcacga gttgcagcaa tgatgataat accacttctg
     8761 aaaaggagaa gatatacgat atgagtggct tcgccaaggg cgccgatgta agttggctca
     8821 cagaaatgga gaaggatggt gtgaaattct ataatcagaa tggcaaggct acagaatgca
     8881 tgaagctcct ccgcgaagag ggaaccaatt ccatccgtct gcgtgtctgg gtaaatccgg
     8941 aaggtggctg gtgtggcaag gatgatgtca tcgccaaggc ttggcgagct cagcagctcg
     9001 gcttccgtct gatgattgac ttccactatt ctgatacctg ggctgacccg ggcaaccaga
     9061 aagttcctgc tgcatggcag ggctatactg ccgaacagat gaaacaggct gtggctgatc
     9121 ataccaagga tgtgttgaaa gccttgaagg atagaggtgt aaccaacgta gagtgggtac
     9181 aggtgggtaa tgaaacccgc gacggtatgc tctggaatag cgatgaggct gtaaccggtc
     9241 aggtatctaa gaatgcagcc aactttgctg cttatatcaa cgtcggatat gatgctgtaa
     9301 aggaagttta tcctaatgca aaggtcatcg tgcatgtaga tcaaggacag gacctcggtg
     9361 gtcttacctg gctctacgat aagctgaagg aaaatggtgg aaaatgggat gtcatcggac
     9421 tgtctcttta tccggaagat gataactggc agagttatgc agagagttgt ctctctaaca
     9481 tccagactct ttcttccatg tatggcaagg acgtcatcat ctctgaaatc ggtatgtggt
     9541 ggggctctga ccaggctgcg ccattgatga agaagatggt ggatggctgt aagaagattt
     9601 ctacctgcga aggtgtcttc tattgggaac cagaagttta caacaactgg aagccagcca
     9661 actatatcac tctgggttgg tctgcatata ccaagggagc gttcgacaac agcggcaaac
     9721 caactgccgt atttgatgca tataaaaata ttaaatagca atatgatcaa gagaagcatt
     9781 actttcatga ctcttgccct ggcactcact acgctgtgcc aggcgcagag ccgaaaaata
     9841 ataaacctgc cttcatggga cttctcccgt gatggcaagg cgtggcagca ggtagctgtt
     9901 ccgcacgatt gggctatctc gggacctttt gataagaaat gggacctgca gatggtggcc
     9961 atcgagcaga atggcgaaac cgaaaagacc gagaagtcgg gacgttctgg tgcgctgcct
    10021 tggataggtg agggtatgta taagatgaac tggacggccc ctaagggcta taagcgtgcc
    10081 gtactcgtct tcgacggtgc catgagccag cctgttgtca gcgtcaatgg caaggaggca
    10141 ggcagatggg cttacggcta caatgccttc cgcatcgaca tcacaccttt catccagttt
    10201 ggtaagagta atctcatcga ggtacatctt aataatgtag aggagagcag ccgatggtat
    10261 ccgggaggcg gtctttaccg tccggtttcc gtagaactct atggcaacga gaacttctct
    10321 acctgggata ccttcgtgcg taccctgaaa gctgataagc aggaagctga agtagaagta
    10381 aatgcgctgc ttgaaggaaa gactggtaag tcgggcaaga ccgtcatcgc cctgttggat
    10441 gaggctggca agaaggttgc cgagcagatc gtcacaggtg ccaatcctga aatcaagact
    10501 actctgaagg tggcgaatcc tcagctctgg tctccggaat ctccttacct ctatcaggta
    10561 aagcttaccc gatatgaagg aaagaaggtg gctgatgtgc agaccctgaa gaccggtatc
    10621 cgcaccattg ccgtatccaa gaataatggt ttccagctca atggcatcac ccgcaaactg
    10681 aagggtgtct gtctgcacca cgacctcggt ccattgggcg cagcagagaa caaggctgcc
    10741 ctcatccgcc agatcaagat gatgaagcag atgggttgcg acgccatccg tactgcccac
    10801 aacatgcctt ctaccatgca gatggaactc tgcgattccc ttggtatgat ggtaatggca
    10861 gagagtttcg atatgtggat ttatcctaaa tgcaagaatg gctatgccaa gttcttcaag
    10921 gagtggagcg ataaagatat cacaaatctg gtaaagcacc accgcaacca tcctagcatc
    10981 gtgatgtgga gcatcggtaa cgagattcct gaacagtgga gcaaggaggg tatggagata
    11041 gccaagcatc tgcaggatat ctgtcatcag tacgaccctt cccgtcctgt tacccagggt
    11101 atggacaagg ctgaggatgc cttgaagagc ggatttgccc aggtaatgga tgtaccgggc
    11161 ttcaattatc gcgtacataa atattttaag aatatagagc agttgcctca gggcttcctc
    11221 ctcggttctg aaaccgcctc tacggtcagc tcccgtggcg tatacaagtt cccagtagtg
    11281 gtaagtgata aggctaccta tcctgatggt cagtgctcaa gctatgacac cgaatattgt
    11341 tcctggagta atcttccgga tgatgactgg aagatgcagg atgactacag ttgggtaatc
    11401 ggtgagtttg tatggaccgg ttacgactat ctgggcgagc cgactcctta cgatacctac
    11461 tggccaagcc gtagcagcta cttcggcatc tgcgaccttg ccggacttcc aaaggaccgc
    11521 tactatatgt atcgtgcccg ctggaacgag cagcagcata ccacccatct tctccctcac
    11581 tggaactgga agggcaggga aggacaggtt acccctgtat attgctacac cgatggtgtg
    11641 gaaggcgagc tttttgtaaa cggcaagagc cagggcagag ttcgcaagga caagtcaagc
    11701 cgtctggacc gctatcgcct ccgctggaac aacgtgaagt atgaaccggg tgagattcgc
    11761 gtggttacct acaaccagta tggcgataag ataggtgagg acgtgaagcg tactgccggc
    11821 gagcctgcac agatgaagtt cagtgtagaa actcctgatc atgagcctat tgcctgcatg
    11881 gtagaaggct gtaccgatga gcacaatgtg cttctgaatg ccgatggcaa cgaccttgct
    11941 ttcatcaccg tgagtctgca ggataaggac ggcaatgaat gtccgcttgc cgatgacgaa
    12001 ctgaccttcg aggtgagtgg tgccggcact ttcaaggcag cctgcaatgg cgatgccaca
    12061 tcgctcgaac cgttcaccca gcctcagatg aaactcttct ccggcaaact cgttgtcatc
    12121 gtacagagta gcaagcagaa gggcgacatc atcctgaagg taaaagacag caagcgaaat
    12181 atcgaacagt cgattacgct ccaggcaata taagatgata ggcacggatt acactcgtaa
    12241 tccgtgcctt atttatactc ttggcatgat acttatgaca ctccctcttc ttttttttat
    12301 aaattttctc cgatattttt tggcgttatc atgaatattg tgtaaatttg cacacatgaa
    12361 aaagtcaaaa gaacctatat ctcgcatcag catggcaatt gctatgctct ttgtttcgct
    12421 cgccatccat gcgcagcgtt tcgtaaacac ctcccttgaa ggtgctcaga cagtttgtgc
    12481 cattacccag aacagtgatg gaatgatatg gctcggaacc gacaacggtc tttacagcta
    12541 tgatggttat catggctacc gccattttca ggaacataca ttcagcaata cgcgtgtcaa
    12601 tgcgctcggt ttcgagggca ggatgctcta tctggctact ggcaacggca tcctgaaatt
    12661 cgatacgcaa tcttatcaat atgccgaaac accagccatg aaggcttttg ccgataaggc
    12721 gaagcgcaag cagctgaagg aactccgtgt gctcgatatc aagggtggta agactgactt
    12781 cggtggcgat gtctatgcct tgctctctac tccgcgaggc ttgctcgtag gttcgctcgc
    12841 tggtctgcat ctcggcagca gactgattcc gctccgtgcc ggagaacagc cgctggtcaa
    12901 tgcgctcgcc tatgatgcca agcgccgatg ctactggatt ggtaccgagg gcgctctcta
    12961 ttgtgccgat ctgcaactca ggaacttctc acagattcca gctctgaacg gcaattccat
    13021 caaatgtctt tctacagatg ctgccggcaa tctctatatc ggaacagata acggactcta
    13081 tcagatggca ctctccaatg ccatcaccca ttatatccac gattcccgcg atgatacttc
    13141 tattcctaac aacatcgtct gggcttgctt tgtagataaa tggcagaatg tatgggtggg
    13201 tactgacaat ggactctccc gtctcactac ccatacctat taccgctatg tatcgctcga
    13261 taagattacg atgtcgggtg agggcaactg tctccatgcc atgctccaga cccgtaacgg
    13321 cgaatggtgg atgggcggta ccaacggtct gattcatttc acccggaatg ctgaaggcta
    13381 tcagaatgtg gcatggtaca agcagaacag ctctgccttc ccgctgagtc ataaccgtgt
    13441 acgcaagatt tatgaagacc atgacggcga tgtatggatc tgtaccgacc atggcatcaa
    13501 cttctatgac cgcagttccc gtcagatgcg caacttcatc gtctacgata agagtggcaa
    13561 gtattctact gcctgggctt acgatattct ccaggacaag aagggcagaa tatggatggg
    13621 ttcttatcag ggcggtgtct tcgtgatgga taaggcggct cttctgagcg gcaagaccat
    13681 agccgactgg cattactcgg acaagggaaa gaatgcgctc tctggtctgc atgtaggtca
    13741 gatggtgatg gatggaaagg gtaggatttg ggtttctacc tatacccgtc tcgactgcat
    13801 caatccgaac gatatgaagg tggtgcaggt agctaactca gatgttgtca actatctgat
    13861 ggcagacagc aagggcaata tctggatggg tgataacaat tcggtaacct gttattatgt
    13921 agccggtact gtgctgggta acagcttttc ttccaagaca tggcagattg gtggcaaggt
    13981 aagtaccatg tgcgatgtgg aaggcaagat atgggtggta tcgggcaatg aatgctgcgt
    14041 aatcaatcct gagggcgaga gccagcgctt catgattcct tctgttgtgc cgctcaacat
    14101 ctgttacagc agacagactc atgaggtaat gatgggcggt aatgacggct atgtagcaat
    14161 cagttccgat gtagcacaga agcctaagct gtttacccgc ctgatgctgg caggaatcat
    14221 cgtaaacgga caggcttgtg aggagcgtgg agaaatcctg aaactgaaga gtgatgagaa
    14281 taacttcacc ctgcagctca ccgaccttcc ttttgctgat catccttcag ccgtttatgc
    14341 ttacaaactc gaaggcagcg accacgactg gcattatctg aactcaggca atatcgatat
    14401 cacctacaat ggtttgtcgt atggcgatta tcatcttacg gttcatgccg tggatggtga
    14461 aggcaagata gctgccgagg tttattcgct cgacatctcc gtcctgccgc cttggtatct
    14521 ctccttctgg tgcaagctct tctatatcac ggcactcatc gtgtttgtgg catggctggt
    14581 aagctggtat tttctgcgca aggaactggc tgatgcccag aagcagaagg ctgaggtgtt
    14641 ggagcaggta cagatgcgta ttgacttctt caaccgtctg acggaagatt tgaaggctgc
    14701 cgtagctcat cgttcatttg atgaggtgac agctctgatg gtccgctacc tgaatgtgaa
    14761 gatgccagag gagccatcag ctgttgctac tggcttatcc gcttcgcctg aatctgagcc
    14821 tgagtcatca tcctcagaac cagttcctgc acgctctgaa tcttctgaag aggaaaagaa
    14881 ggaagtagat gtcagcgagt tggatgtgtc agacaagcgt ctgctcgaag agatcaatga
    14941 agcgatagag aagcacatga tcgattctga cttcaatgtt tcgatgttgc aggatgtgat
    15001 aggcataggt ggcaaacagc tgtatcgcaa gatgaaggcg ataacgggcc gtacgcctgt
    15061 agagtatatc cgtgagatgc gtatgaaccg tgctgccgag ctcctgagcc agggcaagtt
    15121 cagtgtttcc gaggtgatgt ataccgttgg tttttccaac agcagttatt tctccaaatg
    15181 cttctccaag gagtatggca tgactcctac ggaatacatg aaggtgaaga ggtaa
//
