LOCUS       NZ_CP102288            10565 bp    DNA     linear   CON 29-JUL-2024
DEFINITION  Segatella copri DSM 18205 chromosome, complete genome.
ACCESSION   NZ_CP102288 REGION: 2366674..2377238
VERSION     NZ_CP102288.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20222705
            Assembly: GCF_025151535.1
KEYWORDS    RefSeq.
SOURCE      Segatella copri DSM 18205
  ORGANISM  Segatella copri DSM 18205
            Bacteria; Pseudomonadati; Bacteroidota; Bacteroidia; Bacteroidales;
            Prevotellaceae; Segatella.
REFERENCE   1  (bases 1 to 10565)
  AUTHORS   Cheng,A.G., Ho,P.Y., Aranda-Diaz,A., Jain,S., Yu,F.B., Meng,X.,
            Wang,M., Iakiviak,M., Nagashima,K., Zhao,A., Murugkar,P., Patil,A.,
            Atabakhsh,K., Weakley,A., Yan,J., Brumbaugh,A.R., Higginbottom,S.,
            Dimas,A., Shiver,A.L., Deutschbauer,A., Neff,N., Sonnenburg,J.L.,
            Huang,K.C. and Fischbach,M.A.
  TITLE     Design, construction, and in vivo augmentation of a complex gut
            microbiome
  JOURNAL   Cell 185 (19), 3617-3636 (2022)
   PUBMED   36070752
REFERENCE   2  (bases 1 to 10565)
  AUTHORS   Cheng,A.G., Jain,S., Meng,X. and Fischbach,M.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUL-2022) Chem-H, Stanford University, 290 Jane
            Stanford Way, Stanford, CA 94305, USA
COMMENT     REFSEQ INFORMATION: The reference sequence is identical to
            CP102288.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2021
            Assembly Method        :: Unicycler v. 0.4.8
            Assembly Name          :: Prevotella-copri-DSM-18205-MAF-2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 100x
            Sequencing Technology  :: Illumina HiSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_025151535.1-RS_2024_07_29
            Annotation Date                   :: 07/29/2024 03:59:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,718
            Genes (coding)                    :: 2,663
            CDSs (with protein)               :: 2,663
            Genes (RNA)                       :: 78
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 13 of 55
            Pseudo Genes (incomplete)         :: 44 of 55
            Pseudo Genes (internal stop)      :: 9 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10565
                     /organism="Segatella copri DSM 18205"
                     /mol_type="genomic DNA"
                     /strain="DSM 18205"
                     /host="Homo sapiens"
                     /culture_collection="DSM:18205"
                     /type_material="type strain of Prevotella copri"
                     /db_xref="taxon:537011"
     gene            complement(1..1728)
                     /locus_tag="NQ544_RS10075"
                     /old_locus_tag="NQ544_10075"
     CDS             complement(1..1728)
                     /locus_tag="NQ544_RS10075"
                     /old_locus_tag="NQ544_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670650.1"
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="WP_040553491.1"
                     /translation="MNKMLLVAALAATTLSAQAEQKKGPAWLSDALFYQIYPSSYMDT
                     DGNGIGDLPGITSKLDYIKSLGVNALWLNPIFESGWFDGGYDVIDFYKVDPRFGTNTD
                     LVTLVNEAHKRGMKVCLDLVAGHSSTKCAWFKESSEKDPNQRYSDYYIWMNDIPESEK
                     KEIEARHQEASPESSTRGRYVEANAPRAKYYEKNFFECQPALNYGFAHPDPNHPWEQS
                     VDAPGPQAVRREIRNIMAFWFDKGVDGFRVDMASSLIKNDPDKKEVSKLWNEMRAWKD
                     KYYPETVLISEWANPQQAIPAGFNIDFYIHFGLKGYASLFFDRKTPWGKWEQSYQNCY
                     FDKQGKGSLTEFSENYTKAFNATKNLGYIAVPSANHDYQRPNIGTRNTPDQLKVAMTF
                     FLTMPGVPFIYYGDEIGMKYQMNLPNKEGSNERSGTRTPMQWTKGKNAGFSTSAPEKL
                     YFPVDTENGKLTVEAQQGDQNSLLSYTRKLTALRHSAKALDNEGDWKLLNQKGQEYPM
                     VYERTLGDEKYVVVVNPGAKAASLNITSVGGKAVSVLSTGKVVYKSGKKTDAIKASGI
                     SAAVFKVAK"
     gene            complement(1795..3897)
                     /locus_tag="NQ544_RS10080"
                     /old_locus_tag="NQ544_10080"
     CDS             complement(1795..3897)
                     /locus_tag="NQ544_RS10080"
                     /old_locus_tag="NQ544_10080"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009162199.1"
                     /GO_function="GO:0030246 - carbohydrate binding [Evidence
                     IEA]; GO:0016787 - hydrolase activity [Evidence IEA];
                     GO:0046872 - metal ion binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="WP_006848444.1"
                     /translation="MKKLFLLASLLMAFGISADAGDITSPNGQIKVNFTLDGTVPTYS
                     VTYQGKTIIKPSRLGYQLAKGGKDGKDLLSDFSVINEKTSTFDETWTPVWGENKSIRN
                     HYNDMLVELKQNSTDSYMNVRFRVYDDGVGLRYEFPQKGNLNYFTIKEERTEFVMTGD
                     HTAWWIPGDYDTQEYEYTKTRLSGIRPALHAAVSSNLSQTVFSDTGVQTSLQLKTDDG
                     IYINLHEAALVDYPAMHLNLDDKTMTFTSWLTPDAQGMKGYMQTPFKSPWRTMVITDD
                     ARKVLSSNLILNLNEPCKIKDTSWIHPVKYVGVWWEMISGKGEWAYTHDYPTVKLDGT
                     DYVHAKPSGKHSANNANVRRYIDFAAAHGFDAVLVEGWNIGWEDWSGYMKEKVFDFLT
                     PYPDFDIKALNEYAHSKGVKLIMHHETSSSVMNYEKYMDRAYQLMKDYGYDAVKSGYV
                     GNIIPRGEHHYSQLEINHYLHAVTRAADYHIMVNAHEAVRPTGLCRTYPNLIGNESAR
                     GTEYQAFGGTKPGHTAILPFTRLQGGPMDYTPGIFEMDCANGSHCNSTICGQLALYVT
                     MYSPLQMAADFPENYEKHMDAFQFIKDVAVDWDKSIYLEAEPMEYITAARKAKGSDNW
                     FVGGVTGMKAHQSTVKLDFLEKGKKYVATIYQDAKNANYKTNPQAYVITKKTVTSKSV
                     LKLNSVAGGGFAISLLAQ"
     gene            complement(3925..5652)
                     /locus_tag="NQ544_RS10085"
                     /old_locus_tag="NQ544_10085"
     CDS             complement(3925..5652)
                     /locus_tag="NQ544_RS10085"
                     /old_locus_tag="NQ544_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="WP_006848445.1"
                     /translation="MKQYIIGSMLLSSILLASCSLDETPRSKFSEEEAFSTSKLVYVN
                     TVANVYSSIGNGLYGSDGGSVHTFQEFSSDASMIPGRQGDWVDGGTWQNIFLHNFESS
                     VSKYNDVWNNLYRVIGLANSSIDRLNKYLGEHPEYAEYVYELRALRAVYYYYVMDLFG
                     QVPLVVSSQVSANEVNQSNRSDVFRFVTSELAECIPHLSDGKSQNEGEYYGRITKAVA
                     YMCMAKCAINAPVYTIDDTTPTSYSAFVGTDKSGKATASEEQGKTVSEMGKNINITLD
                     GETRNAWETAVYCADQIASLGYRLQPSYADNFIVANQNSVENIWTRPNDCVNYKIEDY
                     NIVRTLHYNHGGAIGYQGWNGACSSKQQMQVYGYGTANPDPRLKLNFYTDKDYMEETG
                     KAVDDGATDKPLEYMPLAVKVDFSAADDPHAMKCAGARMKKYEFDKSTTQQYSFNNDL
                     VIWRYADALLLKAEAEYRMGNKAEALTIVNEVRDRVAATPRTELTLNDILDERMLELA
                     WEGVRRQDQIRFCTFTEPTADRFNGVTHNASAGDYNDDTQGYTMVYPIPYAVLNLNKK
                     LKQNPGYTK"
     gene            complement(5724..8699)
                     /locus_tag="NQ544_RS10090"
                     /old_locus_tag="NQ544_10090"
     CDS             complement(5724..8699)
                     /locus_tag="NQ544_RS10090"
                     /old_locus_tag="NQ544_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019967781.1"
                     /GO_component="GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="WP_006848446.1"
                     /translation="MDKNNLSKKVGLLLAGCFITFNALGQTTIKGQVVDATGEPVIGA
                     SILVKDTKNGAVSDLDGNYKLDNVKDGSVLVFSYLGYRTQEIPVKGNHVINVSLKEND
                     EVLDEVVVVGYAVGSKRTVSGAVDRIKKEDMNKGVVTSPAEALKGKVAGVIISQAGGD
                     PTSSPSIRVRGTSSLSGGNDPLVIIDGVFADMTMFNSLAPGDIESLTILKDASETAQY
                     GSRGASGVIVVTTAKGKLGYSSLSYNGQFGVNTVYKNLDLMSASEYRETAKSLGLTYT
                     DMGGDTNFLEEIERNVGLTQNHNISFSSGTDTSSLRASLGFVLRQGALKNSDMKNFTA
                     KLDGTQYLFDKHLKLELGIFGSQRNSNIQYDMHKMFYSATAYNPTYPNVRNDKGEWDE
                     DLLANEVWNPLGLLDIDDFYKDASVNVHGKATVNIIDGLTVSAFGSYNYWNRDNKFYI
                     PNNIQMGKLNGNGWAYIANTNRKDYMGNVMVNFSKDFGKHHIDALALMEGQKYTYDWN
                     SQEAHGFDTNYFKFNNMKAAANVSWGNLQSNYTEYTLSSYMARVNYMLADKYIATVNV
                     RTDGSSKLGNGQKWGWFPSASLAWVISNEPWMKKIKQIDNFKIRAGYGVTGNQDAIDP
                     YTSLALMEPNGVTTVNGATSTTFAVTSNSNPDLKWEVKTTFDVGFDLSMFKQRLNVTF
                     DWYTSETSDMLYTYTVPVPPFTYDRLLANMGTMTNMGFELAVRGDIIKTKDFTFNSGL
                     NFSYQKNKLKKLSGTYKGQPMTTSEHISVATVGAAGLTHNNGVTYLIEGQPVGVFYLP
                     HCTGIDENGQYIIEDLDDNGTIDTGDSGDRKVCGQAIPKYFLGWDMSFKYKNWDLTMQ
                     FNGAFGHKIYNATSMTLNNMSNFPTYNILSGAQHLNNGKGIHDVQISDYWLEKGDYMN
                     FEYASLGYTFTKDMLKWKFINNIHLALSVNNICTLTGYKGLTPMINSATISGGNLGVD
                     DKNIYPLSRTYTLSLSVNF"
     gene            8928..10565
                     /locus_tag="NQ544_RS10095"
                     /old_locus_tag="NQ544_10095"
     CDS             8928..10565
                     /locus_tag="NQ544_RS10095"
                     /old_locus_tag="NQ544_10095"
                     /inference="COORDINATES: protein motif:HMM:NF039744.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6377 domain-containing protein"
                     /protein_id="WP_006848447.1"
                     /translation="MRHFITIVLLILLPLCAKADNSQLYKQLDAALEKRAHYVEVKEK
                     SLNDIKQGAKYVTSNEDKLKLYEQLANGYKAYEYDSAMTYVKKGLVLAQKSNNILYYK
                     RFQLSQTSLLITRGFYAEAKNIMQKIEPKEEDPLDYQFQYYYTLYGLYNNWSTYCENN
                     EFSKIYDQQKVEYLKKTLELSPKKDAFYYYLLGELYYYSNHSNNNKTILYYKKALSME
                     KPDSRLHAMTAFALSEVYQKANNQKLTEHYLLVAAISDITSATKENVALQNIALFIYK
                     HKTRSLNKAQEYINLSLEDAYAYNNRLRRIEISSKLQMITNAYTDDIRATNSMLYIAL
                     SVIFLLLLGVGLSSLFIRKKNKLLKQKKDEITATSAKMEVLNSQLHLINDELKDTNQK
                     RERLIKVYIDLCYKNIERNSKLRTLAVRKIKANQSKELLSLLSSSTNTEKENKEFLTE
                     FDKAFLSLYPTFITELNKQLTESAHIQLKENGEMPPILRVCALLRLGITESSKIAGIL
                     SYSPQTVYNYRSLLKNNAIDKEHFEENVLRLCMVIAD"
ORIGIN      
        1 ttacttagcc accttgaata cggcggcact aatgcctgaa gccttgatgg catcggtctt
       61 cttgcctgat ttatatacaa ccttgcctgt agaaagaaca gaaactgcct tgccacctac
      121 agaggtgatg ttgagagagg cagccttggc accaggattc acgacaacaa catacttctc
      181 atcacccaaa gtgcgctcat ataccatcgg atattcctga cccttctggt tcaggagctt
      241 ccaatcgccc tcattgtcaa gagccttggc tgaatgacgc aaagcggtca gcttacgggt
      301 atatgacaac agagaattct gatctccctg ctgagcctca acggtcaact ttccgttttc
      361 cgtatctaca gggaagtaga gtttttcagg cgcactcgtt gagaagccgg cattcttgcc
      421 cttggtccac tgcatcggag tgcgggtacc agagcgctca ttgctaccct ccttgttagg
      481 gagattcatc tgatacttca taccaatctc atcaccataa tagataaatg gaacacctgg
      541 catggtgagg aagaaggtca tcgccacctt caactggtct ggcgtattgc gggtaccgat
      601 gttaggacgc tggtaatcgt ggttggcaga tggaacagcg atgtagccga gattcttggt
      661 ggcattgaaa gccttggtat agttctcgct aaattccgta agcgaaccct tgccctgctt
      721 gtcgaaatag cagttctgat agctctgctc ccacttgccc catggagtct tgcggtcgaa
      781 gaacagagaa gcatagccct taagaccgaa atggatgtag aaatcgatgt tgaagccggc
      841 aggaatggct tgctgcggat tagcccactc tgaaatcagg acggtctcag gatagtactt
      901 gtccttccat gcacgcatct cattccagag tttggaaacc tcctttttgt cagggtcgtt
      961 cttgatgagc gatgacgcca tatcaacacg gaatccatcc acacccttat caaaccagaa
     1021 agccatgatg ttacgtatct ccctgcgaac agcctgtggt ccaggcgcat ctacgctctg
     1081 ctcccaagga tgatttgggt ctggatgagc aaagccatag ttcaaggctg gctggcactc
     1141 gaagaaattc ttctcataat acttggcacg aggagcattc gcctccacat atctgcctcg
     1201 ggtactagac tccggacttg cctcctgatg acgggcttct atttctttct tctcgctctc
     1261 aggaatatcg ttcatccaga tataatagtc gctgtaacgc tggttaggat ctttctcgga
     1321 agactcctta aaccaggcac acttggtact ggaatgtcct gccaccaggt cgagacaaac
     1381 tttcatgcca cgcttatggg cctcattgac caatgtaacc aaatcggtat tggtaccgaa
     1441 tcgaggatcc accttgtaga aatcgatgac atcatagcca ccatcaaacc atcccgactc
     1501 aaagatcgga ttgagccaga gggcgttcac acccagcgac ttgatgtaat ccaacttgga
     1561 agtgataccc ggcaggtcgc caataccatt gccatcagta tccatatagg aagatggata
     1621 aatctgatag aaaagtgcat cactcaacca agccggtccc ttcttctgtt ctgcctgagc
     1681 agaaagtgtg gttgccgcca aggcagcaac caacaacatt ttattcattc ttttcatgag
     1741 cttcattatt cattcaagaa aaatttcttt cataataact cataattctg cattttattg
     1801 cgcaagaagg ctaattgcga atccgccacc tgcaacggag ttcaacttca agacagactt
     1861 gctggttaca gtcttcttgg tgatgacata agcctgcgga ttggtcttgt agttggcatt
     1921 ctttgcatcc tgatagatgg tagccacata cttcttgccc ttctcgagga aatcgagctt
     1981 tacggtagac tgatgagcct tcatgcccgt aacgccaccg acaaaccagt tatcagaacc
     2041 ctttgccttg cgggctgcgg tgatatactc cattggttca gcctccagat agatagactt
     2101 gtcccaatct acagccacat ccttgataaa ctggaaggca tccatgtgct tctcataatt
     2161 ctcagggaaa tcggcagcca tctgcaacgg actgtacatc gttacataaa gtgccaactg
     2221 accgcagatg gtagagttgc aatgagaacc attggcgcaa tccatttcaa agatacccgg
     2281 tgtataatcc atcggaccac cctgcagacg ggtgaaaggc aagatggcgg tatgtcccgg
     2341 tttggtacct ccgaaagcct gatattcagt accgcgagca ctctcattgc caatcaggtt
     2401 aggataagtg cggcagagac cggtaggacg cacagcctca tgggcattta ccatgatgtg
     2461 ataatcggca gcacgggtta cggcatgcag atagtgattg atttccaact gactgtagtg
     2521 atgctcgcca cgaggaatga tgttgcccac ataaccactc ttcactgcat cgtagccata
     2581 atccttcatc aactgataag ctctgtccat atacttctca tagttcatta cagaagagga
     2641 ggtctcatga tgcatgatga gcttcacacc cttggaatgc gcatactcat tgagcgcctt
     2701 gatgtcgaaa tcaggatatg gagtcaggaa atcgaatacc ttttccttca tatagccact
     2761 ccagtcttcc caaccgatgt tccatccttc taccaggaca gcatcgaaac catgggctgc
     2821 agcgaagtcg atgtaacggc gtacattggc attgttggca gaatgcttgc ccgatggctt
     2881 ggcatgtaca tagtcggttc catccaactt cacggtagga taatcatggg tataagccca
     2941 ctcaccctta ccggaaatca tctcccacca tacacctacg tacttcacag gatgaatcca
     3001 ggaggtatcc ttgatcttgc aaggctcatt gagattgaga atcaggttgg aagacaacac
     3061 cttgcgggca tcatctgtaa tgaccatcgt acgccaaggg ctcttgaacg gagtctgcat
     3121 atatcccttc ataccctgtg catcaggggt aagccaagag gtaaaggtca tagtcttgtc
     3181 atccaggttc agatgcatgg ctggataatc taccagggca gcctcatgca ggttgatgta
     3241 gataccatca tcagtcttca actggagtga ggtctgtaca ccggtatctg aaaagacagt
     3301 ctgtgagaga ttgctgctga cagcagcatg cagggctgga cggatgcccg acagacgggt
     3361 cttggtgtat tcatactcct gggtatcgta atcacccgga atccaccagg cggtatgatc
     3421 gcccgtcatg acaaattcag tacgctcttc cttgatggta aagtagttga gattgccttt
     3481 ctgagggaac tcatagcgca atcctactcc atcgtcataa acacggaagc ggacgttcat
     3541 ataactgtct gtagaattct gcttcaactc taccagcata tcattataat gattgcggat
     3601 cgacttgttc tctccccaaa caggagtcca ggtctcatcg aaagtggaag tcttctcatt
     3661 gatgacacta aaatcagaaa gcagatcctt gccatccttg ccgcccttgg cgagctgata
     3721 acccagacgg ctaggcttga tgatggtctt gccctgatag gtcacactat aggtaggcac
     3781 agtgccatcc aaggtgaagt tcaccttgat ttgtccgtta ggagaagtga tgtctcctgc
     3841 atctgctgag atgccaaaag ccatcagaag gcttgcgagc aaaaagagtt ttttcatatt
     3901 atttcctttt tagtttatga ctgactactt agtatatcct ggattctgct tgagtttctt
     3961 gttcaagttg agcacggcat aagggatagg ataaaccatg gtatagccct gcgtatcatc
     4021 attgtaatca cctgcagacg cattgtgggt cacaccattg aagcggtcag cagtaggctc
     4081 tgtaaaggta cagaatctga tctggtcttg gcggcgtact ccctcccaag ccagttccag
     4141 catacgttca tccagaatgt cattgagcgt aagttctgta cgaggtgtag cagcaacacg
     4201 gtcacgaacc tcattgacaa ttgtgagagc ctcagccttg ttacccatac gatactcagc
     4261 ctcagccttc aggagcaagg catcggcata acgccagatt accaggtcgt tgttgaagct
     4321 atactgctgg gtagtggact tgtcaaactc atacttcttc attctggcac cagcacactt
     4381 catggcatga gggtcatcgg cagcagagaa atccaccttt acagccaatg gcatatactc
     4441 cagtggcttg tcagtggcac cgtcatctac agcttttcct gtctcctcca tataatcctt
     4501 gtcggtatag aagttgagtt tcagacgagg gtccggatta gccgttccat agccatatac
     4561 ctgcatctgc tgcttggaag agcaggctcc attccagccc tggtaaccga tggcaccacc
     4621 atggttataa tgcagggtgc gcacgatgtt gtaatcctct atcttatagt taacacaatc
     4681 attcgggcgg gtccagatat tctccacgga attctggttg gctacaatga agttgtcggc
     4741 atagctaggc tgcaggcggt aacccaagct ggcaatctgg tcagcacaat agactgctgt
     4801 ttcccacgca ttgcgagttt caccatccaa agtaatgttt atatttttgc ccatttcaga
     4861 aacagtcttt ccctgctcct cgctggcagt agccttgccg ctcttgtctg ttcctacaaa
     4921 ggcactgtag ctggttggag tggtatcatc gatggtatag accggtgcat tgatggcaca
     4981 cttagccata cacatatatg ccacagcctt ggtgatacga ccataatatt caccctcgtt
     5041 ctgactctta ccatctgaaa gatgaggaat gcattcagcc aactcagaag tgacaaacct
     5101 gaagacatca gaacggttgg actgattcac ctcgttagca ctcacctgag aagaaacaac
     5161 caatggcacc tgtccgaaga gatccatcac atagtaataa tagacagcac gaagggctct
     5221 caattcatat acatattcag catactccgg atgttcaccc aggtacttgt tgagtcggtc
     5281 gatagaagaa ttggcaagac cgatgacacg atacaggttg ttccacacat cgttatactt
     5341 gcttacagac gactcaaagt tgtggaggaa gatattctgc caggttccac catctaccca
     5401 gtcaccctga cgaccaggaa tcatactggc atcagaagag aactcctgga aggtgtgtac
     5461 cgagccacca tcgcttccat acaatccatt tccgatggaa gaatatacgt tagccactgt
     5521 gtttacatat accagcttag aggtactgaa agcctcctcc tcagaaaact tgcttcgtgg
     5581 agtctcatcc agtgagcaac tagccagtaa tatcgatgaa agaagcatcg agcctataat
     5641 atattgtttc ataactgtaa tcctttatat attaataatg taattcttaa ttagaacttg
     5701 ttatctattc tatgtgaaaa catttagaaa ttcactgaga gcgacagagt ataagtacgg
     5761 ctcagaggat agatgttctt gtcgtcaaca cccagattgc caccactgat ggttgcagag
     5821 ttgatcattg gagtcaggcc cttgtaacca gtgagggtac agatattgtt gactgacaat
     5881 gcaaggtgaa tgttgttgat gaacttccac ttgagcatat ccttggtgaa ggtgtagccc
     5941 aaagaagcgt actcgaagtt catatagtca cccttttcca gccagtagtc agaaatctga
     6001 acatcatgga tacccttgcc attattgaga tgctgtgcac cgctcaggat attataggtt
     6061 gggaaattac tcatattgtt caaggtcatg gatgtggcat tgtaaatctt atgaccgaaa
     6121 gcaccgttaa actgcatagt cagatcccag ttcttgtact tgaagctcat atcccagccg
     6181 aggaagtact taggaattgc ctgtccgcaa accttgcggt cgccactgtc acccgtatca
     6241 atggtaccat tatcatcaag gtcttcgatg atatactgtc cattctcatc aatacctgtg
     6301 cagtgaggca gatagaatac acctacaggc tgtccctcaa tgagataggt tacgccatta
     6361 ttatgtgtca gaccagccgc acctacggtg gcaacagaga tatgctcgct cgttgtcata
     6421 ggctgtccct tgtaggtacc gctcaacttc ttcagcttgt tcttctgata agagaaattc
     6481 agaccggaat tgaaggtaaa gtcctttgtc ttgatgatgt cacctcgaac agccaattca
     6541 aatcccatat tagtcatggt acccatattg gccagcagac ggtcgtaagt gaaaggaggt
     6601 accggcacgg tataggtata aagcatatct gatgtttcag aagtgtacca gtcgaaggtt
     6661 acattgagac gctgcttgaa catcgacagg tcaaaaccga catcaaatgt cgtctttacc
     6721 tcccacttca gatcagggtt gctgttggag gtaaccgcaa aagtagtact tgttgcgccg
     6781 ttgacggtag ttacaccatt aggctccatc agggcgagag aagtataagg atcgatggca
     6841 tcctgattac cggtcacacc atatcctgca cgaatcttga aattgtctat ctgcttgatt
     6901 ttcttcatcc aaggctcatt gctgattacc catgccaagg aagcagaagg gaaccagccc
     6961 cacttctgac cattacccaa cttagaagaa ccatcggtac gaacattgac ggtagcgatg
     7021 tatttgtcag ccagcatata gttgacacga gccatataag agctcagggt atattcggtg
     7081 tagttagact gcagattacc ccagctcaca ttggcagcag ccttcatgtt attgaatttg
     7141 aagtagttgg tatcaaatcc atgtgcctcc tgtgagttcc agtcgtaggt gtacttctgt
     7201 ccctccatca gagccaaggc atcaatgtga tgcttgccga aatccttgga gaaattcacc
     7261 atgacgttgc ccatatagtc cttgcggttg gtattggcaa tataagccca tccgttaccg
     7321 ttcagcttac ccatctggat attgttagga atatagaact tattgtcacg gttccaataa
     7381 ttgtatgaac caaaggcact tacagtgagt ccgtcgatga tgttgaccgt tgccttgcca
     7441 tgaacattga cagaggcatc cttgtagaag tcatcaatat cgagcaatcc cagtggattc
     7501 cagacttcat tggcaagcag atcctcatcc cattcgccct tgtcgtttct tacattagga
     7561 taagtagggt tataggctgt agcagaatag aacatcttgt gcatatcata ctggatattg
     7621 ctattgcgct gagaaccgaa tatgcccagt tccagcttca gatgtttgtc gaagagatac
     7681 tgggttccat ccagctttgc tgtaaaattc ttcatatcag aattcttcaa ggcaccctga
     7741 cggagaacga atcccagaga agcacgaaga ctggaagtgt ctgtaccaga agagaaagag
     7801 atgttatgat tctgtgtgag acccacattg cgctcaatct cctcgaggaa attggtatcg
     7861 ccacccatat cggtataggt cagacccaaa gacttggcag tttcacgata ttctgaagcc
     7921 gacatcagat ccagattctt atagactgta ttgacaccaa actggccatt gtagctcagg
     7981 ctggaatagc ccaacttgcc cttggctgtg gtaacaacga tgacaccgga agcaccacgt
     8041 gaaccatact gggcagtctc agaagcatct ttcaggatgg tgagactctc aatatcgcca
     8101 ggagccagag aattgaacat tgtcatatca gcaaagacgc catcgatgat aaccaatggg
     8161 tcgttaccac ctgaaagaga agaggttcca cgaactcgaa tgcttggcga actggtaggg
     8221 tcgccgccag cctgagaaat gataacaccc gcaaccttac ccttcaaggc ctcggcagga
     8281 ctggttacca cacccttgtt catatcttct ttcttgatgc ggtcgacagc acccgacacc
     8341 gtgcgcttgc tgcccacagc ataaccaacg acaaccacct catcgagcac ctcgtcgttt
     8401 tctttcagcg aaacattgat aacatgattt ccctttacag gaatttcctg ggtacggtag
     8461 cccagataag agaataccag gacagaacca tccttaacat tgtcaagctt gtagttacca
     8521 tccaagtcgg acactgcacc attcttggta tctttcacca gaatgctggc accaatgaca
     8581 ggttcacctg tggcatctac cacctgaccc ttaatggttg tctgaccaag agcattaaaa
     8641 gtaataaagc atcctgctaa cagcagacct actttctttg aaaggttgtt tttatccata
     8701 atctataggt ttataacgat taaaattgtt tgatgccgca aaagtacgtc aaaattcaat
     8761 aggttgttgc gattagtagg tttttggtag attaccatta tctactatcc aatttatcag
     8821 gctgaatatc agtaaattaa agatttctaa aaaaaacacg ttagtagatg cgagattttg
     8881 aaagtttttt attacttttg cagcagaatc tttaaaacat aagaaacatg agacatttta
     8941 taactatcgt actactgata ctactaccac tatgcgcaaa agcagacaac agccagctct
     9001 acaaacagct ggatgctgcc ctggaaaaac gtgcgcatta tgtagaagta aaagagaaaa
     9061 gtctgaacga catcaagcag ggtgccaaat atgtaaccag caacgaggac aagctcaagc
     9121 tttacgagca actcgccaat ggatacaaag cctacgagta tgattcagcg atgacttacg
     9181 taaaaaaagg ccttgttctt gcccaaaaga gcaacaacat cttatattat aaaagattcc
     9241 aacttagcca aaccagcctg cttatcacac gtggattcta tgctgaagca aaaaacatca
     9301 tgcagaagat agaacccaag gaagaagatc cgcttgacta tcagtttcaa tactattata
     9361 cactttacgg actatacaac aactggtcaa cctattgtga gaacaatgaa ttcagcaaga
     9421 tttacgacca gcagaaagtg gaatacctga agaaaacatt agaactgtcg ccaaagaaag
     9481 atgcattcta ttactacctg ctgggagagt tatactatta cagcaatcat tccaacaata
     9541 acaagaccat cctatactac aaaaaagcac tgagtatgga aaagccggac agccgtctcc
     9601 atgccatgac cgcctttgcc ctatccgaag tataccagaa agccaacaac cagaaactga
     9661 cggaacatta cctgctggtt gcagccatct ctgacatcac ttctgccaca aaagaaaacg
     9721 tagccctgca aaacatcgca ctcttcatct acaaacataa gaccagaagt ctgaacaaag
     9781 cccaggaata catcaaccta tccctggaag atgcttatgc atacaacaac cggttgcgcc
     9841 gcattgagat ttcatccaaa ttgcagatga ttaccaatgc ctatacagat gacatcagag
     9901 ccaccaacag catgctctac attgcgctgt cggtcatttt cctgctcttg ctcggagtag
     9961 gacttagcag tctgttcatc cgcaagaaga acaaacttct gaaacagaag aaagacgaaa
    10021 tcacggctac atcggcaaaa atggaagttc tcaacagcca gttgcacctc atcaacgacg
    10081 aactgaagga caccaaccag aagcgcgaga gactgataaa ggtgtatatt gacctgtgct
    10141 ataagaacat agagagaaac agcaaactgc gcaccttggc agtaagaaag atcaaggcca
    10201 accagagtaa ggagctgctg agcctccttt cttcctcaac caatacagag aaagagaaca
    10261 aggagtttct gacggaattt gacaaggcat tcctgtctct gtacccaact tttataaccg
    10321 agctgaacaa gcagcttaca gaatcggcac acatccagct gaaagagaat ggagaaatgc
    10381 cccctatcct ccgtgtgtgc gccctgttaa gattaggcat caccgaaagt tcaaagatag
    10441 ccggcatcct gtcttattcg ccacaaaccg tatacaacta ccgttccctg ctcaagaaca
    10501 atgccatcga caaggagcac ttcgaagaaa atgtgctcag actctgcatg gtgatagccg
    10561 actga
//
