LOCUS       NZ_CP102288             9899 bp    DNA     linear   CON 29-JUL-2024
DEFINITION  Segatella copri DSM 18205 chromosome, complete genome.
ACCESSION   NZ_CP102288 REGION: 3309626..3319524
VERSION     NZ_CP102288.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20222705
            Assembly: GCF_025151535.1
KEYWORDS    RefSeq.
SOURCE      Segatella copri DSM 18205
  ORGANISM  Segatella copri DSM 18205
            Bacteria; Pseudomonadati; Bacteroidota; Bacteroidia; Bacteroidales;
            Prevotellaceae; Segatella.
REFERENCE   1  (bases 1 to 9899)
  AUTHORS   Cheng,A.G., Ho,P.Y., Aranda-Diaz,A., Jain,S., Yu,F.B., Meng,X.,
            Wang,M., Iakiviak,M., Nagashima,K., Zhao,A., Murugkar,P., Patil,A.,
            Atabakhsh,K., Weakley,A., Yan,J., Brumbaugh,A.R., Higginbottom,S.,
            Dimas,A., Shiver,A.L., Deutschbauer,A., Neff,N., Sonnenburg,J.L.,
            Huang,K.C. and Fischbach,M.A.
  TITLE     Design, construction, and in vivo augmentation of a complex gut
            microbiome
  JOURNAL   Cell 185 (19), 3617-3636 (2022)
   PUBMED   36070752
REFERENCE   2  (bases 1 to 9899)
  AUTHORS   Cheng,A.G., Jain,S., Meng,X. and Fischbach,M.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUL-2022) Chem-H, Stanford University, 290 Jane
            Stanford Way, Stanford, CA 94305, USA
COMMENT     REFSEQ INFORMATION: The reference sequence is identical to
            CP102288.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2021
            Assembly Method        :: Unicycler v. 0.4.8
            Assembly Name          :: Prevotella-copri-DSM-18205-MAF-2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 100x
            Sequencing Technology  :: Illumina HiSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_025151535.1-RS_2024_07_29
            Annotation Date                   :: 07/29/2024 03:59:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,718
            Genes (coding)                    :: 2,663
            CDSs (with protein)               :: 2,663
            Genes (RNA)                       :: 78
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 13 of 55
            Pseudo Genes (incomplete)         :: 44 of 55
            Pseudo Genes (internal stop)      :: 9 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9899
                     /organism="Segatella copri DSM 18205"
                     /mol_type="genomic DNA"
                     /strain="DSM 18205"
                     /host="Homo sapiens"
                     /culture_collection="DSM:18205"
                     /type_material="type strain of Prevotella copri"
                     /db_xref="taxon:537011"
     gene            complement(1..1911)
                     /locus_tag="NQ544_RS13890"
                     /old_locus_tag="NQ544_13890"
     CDS             complement(1..1911)
                     /locus_tag="NQ544_RS13890"
                     /old_locus_tag="NQ544_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="WP_040553739.1"
                     /translation="MNRLSILASMLAMACLPMMAQKTGSKVQEKPDPNFQIYLCFGQS
                     NMEGNAAIEDIDRTGVNPRFQAMYAVDDEKAGWKKGQWHTAVPPQARPSTGLTPVDYF
                     GRQMVDNLPDSIKVGTITVAVGGASIDLFDKRTYKAYLKKQPDWMKNFASQYNGNPYA
                     RLIELAKIAKKQGVIKGILLHQGETNNGDANWPNRVKTVYNDILKDLNLKAEDVPLLV
                     GETVQKDMGGKCWAHIAIVDDIAKTIPTAHVISSKGCPQRGDGLHFIAESYRTMGKRY
                     ANMMLALQGIIPDSNYPRVDKDRRAYVKLHAPEAKKVIFDICGKQYEMKKDLDGDWYG
                     VSDPLVVGFHYYFLNVDGVQVVDPASETYFGCCREAGGLEVPEGAEGNYYRPQQGVAH
                     GQVRSVSYYAASQKQFRRAMVYTPAEYETNTTKHYPVLYLQHGMGEDETGWSKQGMMQ
                     HIMDNLIAEGKAEPMIVVMESGDVKKPFVARPGKNVDEERSHYGASFYDVIIKDLIPM
                     VDKTFRTYTDREHRAMAGLSWGGCQTFNTVLPHMDKFSALGTFSGALFGVDVKTCFNG
                     VFADAEKYNKNIRYMFMGCGSEENFGTEKMAQQLKELGIKLDVYVSPGTHHEWLTWRR
                     CFKEFVPHLFKW"
     gene            2273..2431
                     /locus_tag="NQ544_RS13895"
                     /old_locus_tag="NQ544_13895"
     CDS             2273..2431
                     /locus_tag="NQ544_RS13895"
                     /old_locus_tag="NQ544_13895"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="WP_006849123.1"
                     /translation="MIQRCWFDIMRWNDTKAIVRLTKAGTVVPHLFFPYPQTVMDKNP
                     NLKQNPGW"
     gene            2550..5066
                     /locus_tag="NQ544_RS13900"
                     /old_locus_tag="NQ544_13900"
     CDS             2550..5066
                     /locus_tag="NQ544_RS13900"
                     /old_locus_tag="NQ544_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175202.1"
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="WP_006849124.1"
                     /translation="MKTWMCALMLALSTGASAQNPIISGQYSADPTARVFNGKVYLYP
                     SHDIPSPIEKLKEWFCMADYHVFSSTNLTEWQDHGVIVSQDKVPWAQDGSYTMWAPDC
                     VEKDGKYYFYFPAAPKGEEKGFGIGVAVADQPEGPFMPMWKPIEGVHGIDPCVLIDKD
                     GQAYIYWAGAGLHMAKLKPNMTELASEPKLVEGLPEGFKEGPFAFERNGKYYFTFPWV
                     REKDGTETLAYAMAAHPMGPFTFKGIIMDESPTKCWTNHHSIVEYQGQWYLFYHHNDY
                     SPKFDKNRSVRIDSLNFNPDGTIQKVIPSLRGVGLTKARSHIQIDRYSALQGKGIGIE
                     YLDKNNCFAGWKTLFSKSNTALIYNKVDFGNEKVEEITVRAKSAKGGVLVVRADGKKG
                     NIIAKVKIPKSAAWKNVRAQVLHAPQGVHALHVSLQSGADVEVDWLGFDALPWEKGAF
                     ETHQYRNLFAEMGYKQADIDRKVNEVFNDVFYGKNKVYFEVGDSMGYVSDIKNNDVRT
                     EGMSYGMMAAVQFDKKDIFDRLWRWSKKYMQHQEGPYKGYFAWSCKTDGTRNAQGAAS
                     DGELYFVTSLIFASNRWGNDTGINYLKEAQHILDCSMQKAGMDRTAPLINLEHQLITF
                     TPDHWGGKFTDPSYHLPAFYEVWANWANDGRSQFWKECAEKSREFLHKCINEKTGLNP
                     DYCNYDGSLMKTGRLLGDAFRYDSWRVPMNIALDYSWACKDKEWQQKYANTLQNFLYS
                     KGIDSFLDQYNVDGTMVEDILPAGTAPKALRHSIGFVATSAAASLVSNHVKGREFVSH
                     FWNAKHEPDKEGFFDGYYDGLLRLFAFMHLSGRYQIIEPQ"
     gene            5110..5784
                     /locus_tag="NQ544_RS13905"
                     /old_locus_tag="NQ544_13905"
     CDS             5110..5784
                     /locus_tag="NQ544_RS13905"
                     /old_locus_tag="NQ544_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="WP_208855011.1"
                     /translation="MFCLCVFLFGHTSADAQNRNLPADINPYFGPVGKQPVMPNAAGF
                     IQRWLLLEPISMPVKSNVVFTDSYLKEIFHTQYFPKQMETVPKDGAVVKVGKEKLKWH
                     ALDSKLFNVKLFRFATSFKKPKYGVLFWAVTILDCPEEMKDVRLSVGSNGASMWWLNG
                     EEAVMLEGDRRMVRDDVVSKKLTLKKGRNILRGAVINGPGMSDFCVRFIDGQGKPVRN
                     LSVLVK"
     gene            5805..7295
                     /locus_tag="NQ544_RS13910"
                     /old_locus_tag="NQ544_13910"
                     /pseudo
     CDS             5805..7295
                     /locus_tag="NQ544_RS13910"
                     /old_locus_tag="NQ544_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013064013.1"
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
     gene            7362..9899
                     /locus_tag="NQ544_RS13915"
                     /old_locus_tag="NQ544_13915"
     CDS             7362..9899
                     /locus_tag="NQ544_RS13915"
                     /old_locus_tag="NQ544_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621378.1"
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="WP_006849128.1"
                     /translation="MLKNLSLIALLGFAVVGVPSELSAKSVKVAGTQKGAAAKSVTRQ
                     VAQQNVTIEFYSPSIVRILKSDAGLGAPVQKKSYSVILKPQQMKGVQIQENGDIVKIN
                     SSFISVELNQQTGEIRFLSKDGKLLLTDTKTRLEARKDEANKGKYRIEQDFRLADDEA
                     IYGLGQLRDVYMNQRGRKNIELWNHNTYIAIPYFTSEKGYGLYWDNAGKTYFNDVVAS
                     KNNGNHPSRTSFTSEVGTCADYYFMYKDGTQDGVIASIRELTGQATMFPKWAMGFWQC
                     RERYKTSDELAGVLDKYRELKIPTDAIVQDWQYWGCDSNWNAMKFQNPYYINKVGDPA
                     YAKYLPTDMKQMKAQGEPRLKSPEEMVKYVHKNDAHLMISIWASFGPWTEQYRELKKM
                     NALLPFETWPRNSGVMPYDVFNPKARNLYWKYLTHLYQMGFDAWWTDSTEPDHFEKPG
                     DENYQTFDGSWLGVKNAFPLLHNKSIYEHQRAMKGNTKRSLQMTRSGSFGIQHYGTLC
                     WSGDVVASWNEMKNQIPSGLNFSLCGIPFWNTDLGGFFYWEFEQNPKNPAIQELQTRW
                     MQWGTFMPLMRNHCSSPMVSELYEFGKQGDWAYDAMIKAFKLRYRLLPYIYSTAGDCV
                     QNSGSMMRALVMDYAADKKASRLNDEYLFGRNILVKPVTDPLYTWKDKEKKGHTIYPD
                     VRKAAAPVNVYLPKGNKWYDFWSNTQYEGGQDIQRLCPIDIMPVFIKAGTILPFGPEV
                     QYSSEKPWDELEIRVYPGADGKFTLYEDEGDNYNYEKGKFSEIQFVWKEADRTLNIAP
                     RKGSYKGMLQHRRFHIVLVDANSGAGDQPMQASKSVEYDGKAVKIQL"
ORIGIN      
        1 ttaccattta aagaggtgag gaacaaattc cttgaagcag cggcgccagg tgagccactc
       61 atgatgagtg cctggtgata cataaacatc gagcttgatg cccagttctt tcaactgctg
      121 tgccatcttc tcggtaccga agttctcttc cgaaccgcag cccataaaca tataacgaat
      181 gttcttgttg tatttctctg catcggcaaa aactccattg aaacaggtct ttacatctac
      241 gccaaagaga gcaccgctaa aggttccaag ggcagagaac ttgtccatgt gaggcaatac
      301 cgtgttgaat gtctggcaac cgccccatga caatccagcc atcgcgcgat gctcgcggtc
      361 ggtataagtg cggaatgtct tgtcaaccat cggaatcaga tccttgataa tcacatcata
      421 gaatgaagct ccatagtggc tgcgctcttc atctacattc ttgcctggac gggcaacgaa
      481 aggcttcttt acatcgccac tctccatgac cacaatcata ggctcagcct tgccttctgc
      541 tatcaggttg tccatgatgt gctgcatcat tccctgcttg ctccagcccg tctcgtcttc
      601 gcccatgccg tgctgcagat agagcacagg atagtgcttg gtggtgttgg tttcatattc
      661 ggcaggggtg taaaccatgg cgcggcggaa ctgcttctgg gaggctgcat agtaagatac
      721 tgaacgaacc tgaccgtggg ctacgccctg ctgaggacga tagtagttgc cttctgctcc
      781 ttctggaact tccagaccgc ctgcttcacg gcagcagccg aagtaggttt cactggcagg
      841 gtcaactacc tgcacgccat ctacattcag aaaatagtag tggaatccta ctaccagcgg
      901 atcgctcacg ccgtaccagt cgccatccaa atcctttttc atctcatatt gcttgccgca
      961 gatgtcgaag attactttct ttgcctcagg agcatggagc tttacataag ctctgcggtc
     1021 cttatctaca cgcggatagt tgctgtctgg aatgattccc tgcagggcga gcatcatgtt
     1081 tgcatagcgc ttgcccatgg tgcggtagct ttctgcgatg aagtggagac cgtcgccacg
     1141 ctgtggacag ccctttgaag aaatgacgtg ggctgtagga atggtcttgg caatatcatc
     1201 aacgatggcg atatgtgccc agcattttcc gcccatatcc ttctgtacgg tttcgcctac
     1261 gagcagaggt acgtcttctg ccttcagatt caaatccttc aagatgtcgt tataaacagt
     1321 ctttactcgg ttaggccagt tggcatcgcc gttgttggtt tcgccctgat gcaggagaat
     1381 gcccttgata acaccctgct tcttggctat ttttgccagt tctatcagtc gggcgtatgg
     1441 atttccgttg tactgggatg caaagttctt catccagtcg ggttgtttct tgagataagc
     1501 cttataggta cgcttatcga agaggtcgat gctggctccg cccacggcca cggtgatggt
     1561 tcccaccttg atgctgtctg gcaggttatc caccatctgt cttccgaaat agtcaacagg
     1621 agtcagtcct gtagagggtc ttgcctgtgg cggcacggcg gtgtgccatt gtcccttctt
     1681 ccatcctgcc ttttcatcat ccacggcata catagcctgg aatctcgggt tcacgcccgt
     1741 tcggtcgatg tcttcgatag ccgcatttcc ttccatgttc gactgtccga agcagagata
     1801 aatctgaaaa ttaggatcag gcttttcctg aaccttactg cccgttttct gtgccatcat
     1861 aggtaggcac gccatcgcga gcatcgatgc taagattgat aatctattca tagtttatat
     1921 ttaattaaat tattagattg attttcatct tctgttctgt ttgcaaagtt atgaaaagat
     1981 tttcatacgt gctttgtctc atttatcaat aacttttccc tttctctcat tcttatataa
     2041 taaggtgatt cttttttctc gtgtttcaga atatgtttca ttttgtttct tttggggtag
     2101 atgttatttg tgataaagta tttgttactt gagataaaat gcgttttaat gtttttatct
     2161 atttttgcga cgtgtttcag catccttaaa gaaatttttg agaatttagg atgtagcata
     2221 taataatgta gtatttagca actgacaagc aggtaagacc aattggggtg gtatgattca
     2281 gcgttgttgg ttcgatatca tgcgatggaa tgatacaaag gccattgtgc gtttgactaa
     2341 ggctggtaca gtcgtgccac atttgttctt cccttatcca cagaccgtga tggataagaa
     2401 ccctaacctg aagcagaatc ctggttggta atcatgaagt tgttttgtta atatagtgtt
     2461 ttaagtttaa agaactatag attgtttgac aattaggggg tgttttgaaa aaaagcaccc
     2521 ccttttttct tctttaaagg aatttacgta tgaaaacatg gatgtgtgcc ttgatgctgg
     2581 cgttatcgac aggagcttcg gctcagaacc cgattatcag cggacagtat tctgctgatc
     2641 ctacggctcg cgtattcaac gggaaggtat atctttatcc ttctcatgat attccgagtc
     2701 ctatcgagaa actgaaggaa tggttctgca tggcagatta tcacgttttc tcttccacta
     2761 atcttacgga gtggcaggat catggagtca tcgtttcgca ggataaagta ccttgggcac
     2821 aggacggcag ttatacgatg tgggctcctg actgtgtaga gaaagacggg aaatattatt
     2881 tctatttccc tgccgctccg aaaggggagg agaagggatt cggaataggt gtggcggttg
     2941 ccgaccagcc tgaaggaccg tttatgccga tgtggaaacc catagagggt gtgcacggca
     3001 tagatccttg tgtgctgata gataaggacg gacaggctta tatctactgg gcgggagcag
     3061 gtttgcacat ggctaagctg aaaccaaaca tgacggaact tgcctcggaa cccaaactgg
     3121 tagagggatt gcctgaggga ttcaaggaag gaccgtttgc cttcgagcgt aacggcaaat
     3181 attatttcac cttcccttgg gtacgtgaaa aggatggaac cgaaacgctg gcttatgcca
     3241 tggctgccca tccgatggga ccttttacat tcaagggcat catcatggat gagtcgccta
     3301 cgaagtgctg gaccaatcat cacagcatcg tggaatatca gggacagtgg tatctcttct
     3361 atcaccataa tgattattcg cctaagttcg acaagaaccg ctcggtgcga atcgactctc
     3421 tgaacttcaa tcctgacgga acgatacaga aggtgatacc ttcgttaaga ggtgtgggat
     3481 tgacgaaggc acgttcgcat attcagattg accgttattc tgcgctgcaa ggcaagggta
     3541 ttggtattga atatctggat aaaaacaatt gttttgccgg atggaagact ctcttttcta
     3601 aatcgaatac tgcccttata tataataagg tggatttcgg taatgagaag gtggaagaaa
     3661 tcaccgttcg tgccaagtcg gctaagggcg gtgtgctcgt agtcagagcc gatggcaaga
     3721 agggcaatat catcgctaag gtgaagattc cgaagtcggc agcctggaag aatgtccgtg
     3781 cccaggtgct tcatgctcct cagggcgttc atgctctcca tgtatccttg cagagtggag
     3841 ccgacgtaga ggttgactgg ctgggctttg atgcgttgcc ttgggaaaag ggagcttttg
     3901 aaactcacca gtatcgtaat ctctttgccg aaatgggcta taagcaggct gatattgaca
     3961 ggaaggtgaa cgaagtgttc aatgatgtat tctacggtaa gaacaaggtt tatttcgagg
     4021 tgggcgattc catgggatat gtcagcgata taaagaacaa tgatgtgaga accgaaggca
     4081 tgtcgtatgg aatgatggct gccgtgcagt ttgacaagaa ggacatcttc gaccgcctct
     4141 ggcgatggag taagaaatac atgcagcatc aggaaggacc ttataagggc tatttcgcat
     4201 ggagctgcaa gactgatgga acgagaaatg cccagggagc cgcatcggat ggtgaactct
     4261 atttcgtaac ttcgctcatc tttgcatcca accgatgggg caatgataca ggcatcaact
     4321 atctgaagga agcgcagcat attctggatt gcagcatgca gaaggcggga atggaccgca
     4381 cagctccgct catcaatctc gaacatcagc tcatcacctt tacgcccgat cattggggcg
     4441 ggaagtttac cgatccttct taccatctcc ctgcctttta cgaggtttgg gctaattggg
     4501 ctaacgacgg acggtcccag ttctggaagg agtgtgctga gaaaagccgt gagtttctgc
     4561 ataagtgtat caatgagaag acgggcttga acccggacta ttgcaactat gatggcagtc
     4621 tgatgaagac cggtcggttg ctgggtgatg cattccgcta cgattcatgg cgtgttccta
     4681 tgaacatagc gctcgattat tcctgggctt gcaaggataa ggagtggcag cagaaatatg
     4741 ccaatacgct gcaaaacttc ctgtatagta agggcattga ttctttcctg gaccagtata
     4801 atgtagatgg aacgatggta gaagatattc tgccggcagg tacagctcct aaggcactcc
     4861 gccattctat cggttttgtt gccacttcgg ctgcggcttc attggtcagc aatcatgtga
     4921 agggcaggga attcgtcagt catttctgga atgccaagca tgaaccggat aaagaaggct
     4981 tcttcgatgg ctactacgac ggactgttgc gtctcttcgc cttcatgcat ctgagtggcc
     5041 gttatcagat tatcgaacct caataataat gaatagtcta tgtacaacat ttccaataaa
     5101 atagttagat tgttttgttt atgtgtattc ctttttgggc atacctctgc ggatgcccaa
     5161 aataggaatt tacctgcaga catcaatcct tatttcggac cagtcggcaa gcagcctgtc
     5221 atgcctaatg cggctggctt tattcagaga tggttgctcc tggaacccat ctctatgccg
     5281 gtcaagagca atgtcgtttt tactgattct tatctgaagg agatattcca tacgcagtat
     5341 ttccctaaac agatggaaac agttcctaag gatggagccg tcgtgaaggt gggcaaggaa
     5401 aaactgaaat ggcatgcgct ggacagtaaa ctgtttaatg tgaaactctt ccgtttcgct
     5461 acttcgttta agaagcctaa gtatggtgtc ttgttctggg cggttaccat tctcgattgc
     5521 cctgaggaga tgaaagacgt ccgcttgtct gttggctcga atggtgcttc catgtggtgg
     5581 ctcaatggtg aggaggcggt gatgctcgaa ggcgaccgaa ggatggttcg cgatgatgtg
     5641 gtttctaaga aacttacctt gaagaaaggt aggaatatcc ttcgtggtgc ggtaatcaat
     5701 ggtcctggca tgagcgactt ctgtgtccga ttcatcgacg gacagggcaa gcctgtcaga
     5761 aacctgtctg ttcttgtaaa gtagtgcaat caatatagaa agttatgaaa cagaacaata
     5821 tatttttcag atattttagc ccggttgctg ctcagcagaa actttatgtc gctgctttgg
     5881 ttgccttgtt gtcttcctct gcttacgagg cagaagccca ggtgggcgaa cctttcattc
     5941 atgatccttc taccattgcc cagtgtgatg gaaagtatta tacctttgga acgggtgaag
     6001 gcggaatctg gtcggaggat ggctggacct ggcagggtgg tgctgttcgt cccggaagag
     6061 gagcggctcc tgatgtgttg aagattggcg accgttatct tgtagcctac agtactacgg
     6121 gtggtggatt gggaggcagt catcgcggtg atgtcctgac gatgtggaac aaaacgctcg
     6181 atccgaaatc gcctgatttc aaatataccg aaccggtggt ggtcgcctca tccttagatg
     6241 atgaagactg tgatgccatt gatgcgggct tgttgctcga ccctactacc ggcagactct
     6301 ggctcagcta tggtacctat ttcggattta tccgtctggt agaacttgat ccgaagacgg
     6361 gtaagcggat ggaaggcaac gaacccgtca atatcgccat cgactgcgaa gctactgatt
     6421 tgatttaccg caacggctgg tattatcttc tcggcactca tggcacctgt tgcgatggtc
     6481 ctaattctac ctacaatatc gtggtgggac gttcgagaaa gataaccggt ccatacgtag
     6541 ataatgtggg cagagaaatg ttgcagggcg gtggaaagat ggtgattgct gccaacaatc
     6601 tgaagacggg tcccggtcac ttcggacgct atatcgagga agagggtgta gaaaagatgt
     6661 cgttccacta tgagtctgat ttcaggcagg gttgacgaag cgtgttggct atccgtcctt
     6721 tgttgtggaa gaacgactgg cctgtggctg gtgatgaatt tcatgccgga acctacgaga
     6781 tagaatcgga acgaagaggc tatgccctgg agattgccgt agatttcgtg agaatgcaac
     6841 gtgatatcga acctttctgg attaagccga ccaagcctct gaagaatatc gaacctcaga
     6901 ccttgaagga ggtagaggca ggatggccta agggcgaggt aaaggtaaga atgaacgatt
     6961 atatgttccg tcctcatcag aagtggagta tcatgcctgt cggaaagggt ggttatctgg
     7021 gtggccctta ttataagatc tgcatagaag gcactactcg ctatcttacc gcaaccgctc
     7081 agcatgatgt catcgccaaa cctgagttta ctggtgaaga tgcccagctt tggagcatcg
     7141 aacaacttac cgatggcacc tatcgcatca tgccgaaggc agtgccaggc actgaagaga
     7201 aactggcatt ggtttcatta ggcgactgta ccccaggttt ggctcccttc gatttcaaca
     7261 gcgataattc taaatggaat ttcaggcaac aataaattca tgatgatttg acaatcgaga
     7321 agcgtcccaa tcataaactc tctaataaaa taaacagaag tatgctaaag aatttatctc
     7381 tcattgccct tttgggcttc gctgtagttg gtgttccaag tgaactcagc gccaagagcg
     7441 taaaggtggc aggcacccag aaaggtgcgg ctgccaagtc tgtcacccgt caggtggctc
     7501 agcagaatgt aaccatcgaa ttctattctc catccatcgt ccgcatcttg aaatctgatg
     7561 ccggacttgg tgctcctgtt cagaagaaaa gctattctgt catcttgaaa cctcagcaga
     7621 tgaagggcgt tcaaatacag gaaaacggag atattgtaaa aatcaattct agttttattt
     7681 ccgtcgaact gaatcagcag actggcgaaa tccgctttct ttcgaaggat ggaaagctgc
     7741 tgcttaccga tacgaagacc cgtctggaag ctcgcaagga cgaagccaac aagggcaagt
     7801 accgcataga gcaggacttc cgtcttgctg atgacgaggc catctatggt ctgggacagt
     7861 tgcgcgatgt ttatatgaat cagcgtggcc gaaagaacat cgaactctgg aatcataaca
     7921 cctatatcgc catcccttat ttcaccagtg agaagggcta tggtctttac tgggacaatg
     7981 caggaaaaac ctatttcaat gatgtcgtag catccaagaa taatggcaac catccatccc
     8041 gcacttcctt taccagcgaa gtgggaacct gcgccgacta ctatttcatg tataaggatg
     8101 gtacgcaaga tggagtcatc gccagcatcc gtgaactgac cggacaggct acgatgttcc
     8161 cgaaatgggc gatgggtttc tggcaatgcc gtgagcgcta taagacaagc gatgaactgg
     8221 ctggcgtatt ggacaagtat cgcgaactga agattcctac tgatgccatc gtgcaggact
     8281 ggcagtattg gggctgcgac tccaactgga atgcgatgaa gttccagaac ccatactata
     8341 taaataaggt gggcgatcca gcctatgcca agtatcttcc taccgatatg aagcagatga
     8401 aggcgcaggg agaaccccgc ctgaagagcc cggaagagat ggtgaagtat gttcacaaga
     8461 acgatgccca tctgatgatt tccatctggg caagtttcgg tccttggaca gagcagtatc
     8521 gtgaactgaa gaagatgaat gccctccttc cttttgaaac gtggccaaga aacagtggcg
     8581 tgatgccata cgatgtcttc aatccgaagg ctcgcaacct ctactggaag tatcttactc
     8641 atctctacca gatgggcttt gatgcctggt ggaccgattc tacggagcca gaccattttg
     8701 agaagccggg cgatgagaac tatcagacct tcgatggttc atggttgggc gtgaagaacg
     8761 ccttcccatt gttgcacaac aagagcatct acgagcacca gagagcgatg aagggcaaca
     8821 caaagcgttc gctccagatg acccgaagcg gcagtttcgg tattcagcat tatggcaccc
     8881 tctgctggag cggtgatgtg gtggcttcct ggaacgagat gaagaatcag attccatcgg
     8941 gcttgaactt ctcgctctgc ggtatcccat tctggaacac cgacctgggt ggtttcttct
     9001 actgggagtt tgaacagaat ccaaagaacc ctgccattca ggaactgcag acccgctgga
     9061 tgcagtgggg aaccttcatg ccattgatgc gtaaccactg ttcttcaccg atggtaagcg
     9121 aactgtatga attcggaaag cagggcgact gggcttatga tgctatgatt aaggccttca
     9181 agctgcgcta tcgccttttg ccttatatct acagtacggc tggcgactgt gtacagaata
     9241 gcggaagcat gatgcgtgcc ctggtaatgg attatgcggc agacaagaag gcttcccgtc
     9301 tgaacgatga gtatctcttc ggccgcaaca ttctggtgaa gccggtaacc gatcctttat
     9361 acacctggaa ggataaggag aagaagggcc ataccatcta tcctgatgta aggaaggcag
     9421 ctgcgcctgt gaatgtttac ttgccaaagg gtaataaatg gtatgatttc tggagcaaca
     9481 cccagtatga gggcggtcag gatatccagc gcctttgtcc tatcgacatc atgcctgtat
     9541 tcatcaaggc aggtaccatc ctgccattcg gtcctgaagt gcagtatagt tcagagaagc
     9601 cttgggatga gctggaaatc cgtgtctatc ctggtgctga tggtaaattt acgctctatg
     9661 aggatgaggg cgacaactat aattacgaga agggtaaatt ctcggaaatc cagtttgtat
     9721 ggaaagaagc agaccgaacc ctgaacatcg ctccacgcaa gggcagctat aagggaatgc
     9781 tccagcatcg cagattccat atcgtactgg tggatgccaa tagtggagcg ggcgatcagc
     9841 ctatgcaggc aagcaagagt gtggaatatg acggaaaggc tgtaaagata cagctgtaa
//
