LOCUS       NZ_CP102288            14214 bp    DNA     linear   CON 29-JUL-2024
DEFINITION  Segatella copri DSM 18205 chromosome, complete genome.
ACCESSION   NZ_CP102288 REGION: 546421..560634
VERSION     NZ_CP102288.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20222705
            Assembly: GCF_025151535.1
KEYWORDS    RefSeq.
SOURCE      Segatella copri DSM 18205
  ORGANISM  Segatella copri DSM 18205
            Bacteria; Pseudomonadati; Bacteroidota; Bacteroidia; Bacteroidales;
            Prevotellaceae; Segatella.
REFERENCE   1  (bases 1 to 14214)
  AUTHORS   Cheng,A.G., Ho,P.Y., Aranda-Diaz,A., Jain,S., Yu,F.B., Meng,X.,
            Wang,M., Iakiviak,M., Nagashima,K., Zhao,A., Murugkar,P., Patil,A.,
            Atabakhsh,K., Weakley,A., Yan,J., Brumbaugh,A.R., Higginbottom,S.,
            Dimas,A., Shiver,A.L., Deutschbauer,A., Neff,N., Sonnenburg,J.L.,
            Huang,K.C. and Fischbach,M.A.
  TITLE     Design, construction, and in vivo augmentation of a complex gut
            microbiome
  JOURNAL   Cell 185 (19), 3617-3636 (2022)
   PUBMED   36070752
REFERENCE   2  (bases 1 to 14214)
  AUTHORS   Cheng,A.G., Jain,S., Meng,X. and Fischbach,M.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUL-2022) Chem-H, Stanford University, 290 Jane
            Stanford Way, Stanford, CA 94305, USA
COMMENT     REFSEQ INFORMATION: The reference sequence is identical to
            CP102288.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2021
            Assembly Method        :: Unicycler v. 0.4.8
            Assembly Name          :: Prevotella-copri-DSM-18205-MAF-2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 100x
            Sequencing Technology  :: Illumina HiSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_025151535.1-RS_2024_07_29
            Annotation Date                   :: 07/29/2024 03:59:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,796
            CDSs (total)                      :: 2,718
            Genes (coding)                    :: 2,663
            CDSs (with protein)               :: 2,663
            Genes (RNA)                       :: 78
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 13 of 55
            Pseudo Genes (incomplete)         :: 44 of 55
            Pseudo Genes (internal stop)      :: 9 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..14214
                     /organism="Segatella copri DSM 18205"
                     /mol_type="genomic DNA"
                     /strain="DSM 18205"
                     /host="Homo sapiens"
                     /culture_collection="DSM:18205"
                     /type_material="type strain of Prevotella copri"
                     /db_xref="taxon:537011"
     gene            1..3165
                     /locus_tag="NQ544_RS02170"
                     /old_locus_tag="NQ544_02170"
     CDS             1..3165
                     /locus_tag="NQ544_RS02170"
                     /old_locus_tag="NQ544_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272027.1"
                     /GO_component="GO:0009279 - cell outer membrane [Evidence
                     IEA]; GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="WP_006846382.1"
                     /translation="MRKTSQKFSQSHMLCKVALVAIMLVFQAMLGIGAGVLNAQTVKG
                     TVISGSDNEPLIGASVMVQGTKTGAVTDLDGNFTIEAKNGQTLEVSYLGFITQKIKVT
                     GSTINVTLNEDKQSLDEVVVVGYGVQKKKLVTGATVQLKGDDIAKLNTTNPLSAMQGQ
                     TPGVNIVSTSGQPGAAMSVTIRGLGTVGNSQPLYLIDGVGGDITTLNPADIESIDVLK
                     DAASAAIYGAQAANGVVLVTTKSGKEGSSKITYDGYVGWQTLGRKFEMLNSSQYMQIM
                     DEALLNSYMSPIDWTSLSAIRDANGNVYNTDWIDQAVDNGALTTSHSLAFTGGSKTST
                     YSISGGYTGQDGLIGGSDISYYKRYNLRVNSEHKMWNGLVTIGEHVGFVYKDSRGMGT
                     GNIYNNNLRSAFSTSPLVPVYDADGNYYSTVDSDWNKNDGNPYGTMMMNRYNQSKSTS
                     VDANVYVQIEPVKNLKFKTVFGLNYGGSNYRSFTPIYKFTPQSGNGITKVNQSNGNGT
                     SLVWTNTLTYDFDIKEHHISALLGSETTKYDGESTGSYGVNLTAGFDDWEHAYVENTE
                     KGHADRKVSGGPYDATRGQSFFARLGWSWKDRYMVNATMRADGSSKFAKGHRWGYFPS
                     VSAGWTLTEEDFMKSSASWLDFLKLRLSWGQVGNANINCYQYLAPVTTSNTNYNFGAT
                     GGTDAWVMGAYTERLANEKVKWETSEQYNVGLDARFLRQRLSLTLDGYIKSTKDWLVQ
                     APILATAGTGGPVINGGDVKNKGIEVGLSWNDQIGKDFVYSVGANFAYNHNEVGNIPT
                     LDGIIHGATNQIYQNAEEFYRAENGHAIGYFWGYKTAGLFQNQKEINDWIAAGNGIYQ
                     ADVKPGDVKYVDVNHDGVINASDKVDLGNGLPKYTFGFNFSLAWKGFDLSANFTGAAG
                     FQIAQSYRDPSSSQANYSRRILKRWTGEGTSNEIPRVTYGDVGNWLFSDLYLQDGDYI
                     RLQNLTMGYDFKKLISWKGLSKMRLYFQVQNLFTLTKYDGMDPEIGSFNGTDGNSSDS
                     WVSGVDMGYYPHPRTFIVGLNLAF"
     gene            3185..4861
                     /locus_tag="NQ544_RS02175"
                     /old_locus_tag="NQ544_02175"
     CDS             3185..4861
                     /locus_tag="NQ544_RS02175"
                     /old_locus_tag="NQ544_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="WP_006846381.1"
                     /translation="MNKKHILMSALILSSMALTSCDDFLDTSSKTKMNSETAYSTPAA
                     ADMDLVGCYDGWQRTLSDEGVGMYLTAEFASEQAFAGLGLSDAKNNNAIDQFDLTVAP
                     SYTDLFNTDWKNYYQAIYRCNQLIQADATIKWNGDTKAEGRIMGEARALRGILYFDLA
                     RLFGDVPLLLEPSEANIPRTPVKEVYQSIFDDLKYAAANIPADAYPLKDRDSNDGRIT
                     KYAAEALLARAYLYYTGYYGEEHPACSKAEATAAINDVVENGGYELEKNYADFWMPSC
                     TKDASSTGNYAWETTYAGKWYDGKTWKAGQGSLSKEIVLNLKLNSTHDYNGNGDGNTF
                     SVYLGPRNRCATDICIASGWGACPVTPYFVEQFRNDPRFNACVWSCKDAGFTADESDS
                     YEYTGYYTRKYAPMCFADGTRQEVGFQLGEQHQNITYYQDYTIMRYADVLLMHSELNG
                     TADGLNKVRARVGLEPEDYSIENIRKERAIELAFEGVHFWDLMRYGKDGSYAAQEIAK
                     AQNGAKVKNGGVEAATKFAVDNFTSKKGLMQIPNTQITLSGNVLTQNAGW"
     gene            4904..6937
                     /locus_tag="NQ544_RS02180"
                     /old_locus_tag="NQ544_02180"
     CDS             4904..6937
                     /locus_tag="NQ544_RS02180"
                     /old_locus_tag="NQ544_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007219505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_006846380.1"
                     /translation="MKIHNLIYTLLVAMAVFLLASCSPEDFGFGKNTYSPEDLVEGKA
                     YTVTIDGNIVKLESKIPDATPLWITPVGRKSQQALDVELPFAGDYEVTFGVETPGGIV
                     YGEPYKLSLSKNDFSLLNDDKWFLLADKNYKTGEAFPSAETLAAGVSKKWYPCDGNYN
                     IGRCTSPVMYCSPYDPDYDGKGYTADEEANAVYKDILFGSDNWKPNWDPGFQSWLIPE
                     TDPYMDSYMTFSMDAKNGCVATMYRGESGEKGASTGSNMVGKFNLGLGDKTKPTITFN
                     DCYAMHNIGFDAVCSNYTQNIQIVELTPYLLQLVTKRTNSEGNWYLVWNFVSEDVIAT
                     KGACIPKADQELIETVKPILPTFENIHTDLFTADINGDKYVGSKMTFNLDSETPYDFL
                     WWNGSPNVKAWESVIGGKYNTTWAPALGDKAIDNFELTISKASDGSYNYECGEVDGKV
                     KITENTMTFDKEISVFAVAGDKRTIELKGKKFYILGNSTDDQYLTIGIPETTDENGLV
                     NSYLVAKLAYKKIATGPVGPTKVAIDNSIIHNEGIMWVENGCLRIGFHHYGETGKGLF
                     KDVKSVKLKKNQTITVTFKIKGGVTWSKTPKCALIDNNIKQTWEPGAYDLPDAVAVDT
                     NGGETTVSLTNTTGSTQTFTATCLDLSIQLDGFGDYGGSTDNIDIEIVSCTIQ"
     gene            6956..9631
                     /locus_tag="NQ544_RS02185"
                     /old_locus_tag="NQ544_02185"
     CDS             6956..9631
                     /locus_tag="NQ544_RS02185"
                     /old_locus_tag="NQ544_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007219506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_006846379.1"
                     /translation="MKNKILYGIMAFVMGIFMTGCSDDDYSISNTPLLTDESVTTGSA
                     DVTVTSATFHGTVKGLEEQNASAYALGFYYGKSEDNLSEKVAASGSNEFQATVSGMPG
                     DVVYYQAYVTLQGRVTYKGSVQSAIMTDAKAITGEPKDLTANSVILTGKLEKAPQEAT
                     SGIVISGVEGSEKVRAGVRIVAAGINDNYEIKAEGLLPNTTYHYTAYLDLGNGTVYGE
                     DRTFTTAPADFNPDTDLVDLGLSTKWAKYNVGATDEKQLGGLFGFGDMTGFQTSINLD
                     DYASADIYKTDRDVANKVYGSWVTMPTIDEFEELFTECKKEWVEDTENHVAGYKFTGP
                     NGNSIFLPAAGTRTQGNVSGEGLNGYYLSGSINATDNRFAMAYNFDQNAARRTTTPVY
                     QALAIRPVSVAKNVKFDKAKLIGQTWEIDLRLDGSHYKFDGPVYFLGKDDSWATITNN
                     QPVVGNVGCWAADFASNSWTVGGDAKNCQGTMTFNEDGTVKVHQYVAGADGAEGTFQD
                     TEGTYTIDEKNKTVKLSIAPLIPANYIGNVKETKDMDIRILSLTDETMQLAVTRANDN
                     QYMSINYVPGELKNGFTAKLTCYGGDDENTPDAWNSATVNVLGGAAGLKTYTVTFNTQ
                     YPRTNGKVYLLELEGYSAAYPKALVRIDAIKADGNDVKFDANKFFYGDLEGNGNYRVE
                     MANIWGCGHNDSWDGLKDTPFHKDGGETKNETALAFNHTFEVTFTVVSNTSDGTGVYT
                     PTFNAVRGWGEGEAQLFGYNDGSTLKVVKSDKGQYSLENNQFDMTYEGSGFEGGTIMT
                     FVEIADLYGFFPGTHSTLDEFYLDGKAVSYDKSKVVDANENPKYRLELFNCYAATKDN
                     CAFGVKDGDLMRELGFNKSMRAKFTVHSLFAVPQW"
     gene            9706..11256
                     /locus_tag="NQ544_RS02190"
                     /old_locus_tag="NQ544_02190"
     CDS             9706..11256
                     /locus_tag="NQ544_RS02190"
                     /old_locus_tag="NQ544_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="WP_006846378.1"
                     /translation="MKKFKYLYLLLLGIVCGLAFSACSYEDDDYDEPSFKVLNPELSF
                     DGTGGVQTINVQADAQPTASVIEGADWCSVAYKDQAAGTYNFDVTVAASQEDEVTTAT
                     VRIIQGYSRKDVTITRAKKGAVVTPDVPPADMNKTAMEVAQLMYPGWNLGNTLEGGDS
                     KNLWKNAGIETETVWQNAKTTQALIDAVKAAGFKSVRIPCSWVMGHITDAEKCTIDPA
                     WMKRVKEVVDYCIKDGLYVIINQHWDGGWIEHNGMTANADIKTTKAQLTKIWTQIADN
                     FKTYDEHLLFAGMNEPGVGAGEGDIIGVADMSNRIAEYEQTFIEAVRATGGNNAKRVL
                     IVQGPNTDIDKFVANNYMSKIHDSATDRLMVEVHFYDPYQFTDLSEDKDWGKYYLYWG
                     KNNTNGSEAGRTADAKYNEDYVEAQMKKMKTNFFDKGYPVLIGEFGANQRLAIGKDAV
                     HDASVKDYYKAVVTSAINNGCVPMAWDTNGGLPSMTIFNRAGASVSNTNMLESITAGV
                     AAAKWPAK"
     gene            11473..12786
                     /locus_tag="NQ544_RS02195"
                     /old_locus_tag="NQ544_02195"
     CDS             11473..12786
                     /locus_tag="NQ544_RS02195"
                     /old_locus_tag="NQ544_02195"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="WP_006846377.1"
                     /translation="MKKIVMIILAAAHFAWAGAQVKSMDGRISKMKLTATELAHYMAP
                     GVNLGNTMEACDWNDVFTNQAGLKSETSWQNDKTTESYIRSLKQQGFNSLRIPTSWVA
                     GHLTDKENMTIDPVWMKRIKEIVNYGLNAGLCVIINEHWDGGWMEHDAFTSGANVTEY
                     KEMFRKLWTNIAKEFKTYDQRVLFAALNEPGVGGASPQVQGDMLAPDSKEFADRLLAY
                     EQVFIDAVRATGGNNSSRVLIVQTPKTEIDLAAKDSYDITRLKDKVKNRLMAEVHFYD
                     PYIFTLMDKDADWGKVALYWKGHAPADDQGRTVNTIWYNNKNVDAYQHITNQVMKMKQ
                     KFVDLGYPVVIGEYGANRKDASLFGGNQEKHNESMMAWYGAVTAEMMKAGLIPYVWDI
                     NVQPLPHMTIFDRKKQVVSDSYIFKGVMQGAAEGMEDYLRIYPKP"
     gene            12928..14214
                     /locus_tag="NQ544_RS02200"
                     /old_locus_tag="NQ544_02200"
     CDS             12928..14214
                     /locus_tag="NQ544_RS02200"
                     /old_locus_tag="NQ544_02200"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007410996.1"
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 5 family protein"
                     /protein_id="WP_006846376.1"
                     /translation="MKKIFVFLMMALATCNISAKSNFVQVKDGHFVRDGKPYYYVGTN
                     FWYGAILGSEGQGGNRERLCKELDKMKEMGFDNLRILVGSDGKRGVKTKVEPTLQEAP
                     GVYNDTILAGLDYLLMEMGKRKMLAVLYLNNSWEWSGGYGYYLEQAGLGQAPRPNEDG
                     YPAFMNFVAKYASCEKAHQLFYDYVKFILTRTNRYTKKKYKDDPAIMSWQIGNEPRAF
                     SKEQLPAFEKWLGEAGKLIRSLDKNHLISIGSEGKWGCEGELNCWERICADKNVDYCN
                     IHLWPYNWSWARKDHLQEDLGISCKNTKEYIDEHLEECGRIRKPLVMEEFGYPRDGFS
                     FSTSSTTEARDGYYKYVFSLVGDNAASGGYFAGCNFWGWGGFANPKHEQWQVGDDYTG
                     DPAQEAQGLNSVFSTDKSTLDVVKTQVDRMKKIGKL"
ORIGIN      
        1 atgagaaaaa catctcagaa attctcgcag agccacatgc tctgcaaagt tgctctggta
       61 gccatcatgt tggtgttcca ggcaatgtta ggaatcggag cgggtgtcct caatgctcag
      121 acggttaagg gtactgtcat ctctggcagt gacaatgaac cattgattgg tgcaagtgtc
      181 atggtgcagg gtacaaagac gggtgccgtt actgacctgg atggtaactt taccattgaa
      241 gcaaagaatg gacaaacact cgaagtttcg tatctgggct ttatcaccca aaagattaaa
      301 gttacaggtt cgacaatcaa cgtaacctta aatgaagaca agcagtcact tgatgaagtt
      361 gtcgtagtag gttacggtgt tcagaagaaa aagctggtga cgggtgctac cgttcagttg
      421 aagggtgacg acattgccaa gcttaacacc accaaccctc tttctgccat gcagggacag
      481 acacctggtg ttaacatcgt ttctacttct ggtcagccag gtgcagccat gagtgttacc
      541 attcgtggtt tgggtaccgt aggaaactct cagcctcttt acttgattga tggtgtgggt
      601 ggtgatatca caaccttgaa ccctgccgac atcgagagca tcgacgtatt gaaggatgct
      661 gcttctgctg ctatctatgg tgcacaggct gccaatggtg tagtccttgt tacaaccaag
      721 agcggtaagg aaggttcttc caaaattaca tacgatggat atgtaggttg gcagactctg
      781 gggcgcaagt ttgaaatgct caattccagc cagtatatgc agattatgga tgaggctttg
      841 ctgaattcat atatgtcgcc tatcgactgg acttctctca gtgctatccg tgatgcaaat
      901 ggtaatgttt ataatacaga ctggattgac caggctgtag acaatggtgc cttgaccact
      961 agccacagtc tggcttttac cggtggtagc aagacttcaa cttattctat ttctggtggt
     1021 tataccggac aggacggtct tattggcggt tctgacattt cttattataa aaggtataac
     1081 cttcgtgtca attctgaaca taagatgtgg aatggattgg taactattgg tgagcacgta
     1141 ggttttgttt ataaggatag ccgtggcatg ggtaccggca atatctataa caacaacctc
     1201 cgcagtgcgt tctctacttc tccactggtt cctgtttatg acgcagatgg taactattat
     1261 tctaccgttg attcagactg gaacaagaat gatggtaacc cttatggtac catgatgatg
     1321 aatcgctaca accagagcaa gagtacttcg gttgatgcga atgtatatgt gcagattgaa
     1381 cctgtcaaga acttgaagtt caagacagta ttcggcttga actatggtgg ttctaactat
     1441 cgttcattta ctcctattta caagtttact cctcagagcg gtaatggtat caccaaggtg
     1501 aaccagagca atggcaatgg tacttctctt gtctggacca ataccctgac ttacgatttc
     1561 gatatcaagg agcaccatat cagtgctttg ttaggtagtg agactactaa gtatgatggt
     1621 gagtcaacag gcagttatgg tgttaacttg actgctggct ttgatgactg ggagcatgcc
     1681 tatgtagaga atacagagaa gggacatgct gaccgtaagg taagtggtgg tccttacgat
     1741 gctacccgtg gtcagtcttt cttcgcccgt ttgggttggt cttggaagga tcgctatatg
     1801 gtgaatgcta ccatgcgtgc cgatggttct tctaagtttg ccaagggaca tcgttggggt
     1861 tatttcccat cagtttctgc cggttggact ttgactgagg aagatttcat gaagtcttct
     1921 gcttcctggt tggatttcct gaagctccgt ctctcttggg gtcaggttgg taatgctaat
     1981 atcaactgct atcagtatct ggctcctgtt accacatcaa ataccaacta caacttcggt
     2041 gctacaggtg gtaccgatgc ttgggtgatg ggtgcttata cagagcgtct ggctaatgag
     2101 aaggtgaagt gggagacttc agagcagtat aacgttggtt tggatgcccg tttcctccgt
     2161 cagcgtcttt ccttgactct cgatggttat atcaagagca ctaaggattg gttggttcag
     2221 gctcctatcc tggctacagc tggtaccggt ggtcctgtca tcaatggtgg tgatgtgaag
     2281 aacaagggta tcgaggttgg actttcatgg aatgaccaga ttggcaagga ctttgtttat
     2341 agtgtaggtg ccaacttcgc ttataaccac aatgaagtag gtaatattcc tacattggat
     2401 ggtatcatcc atggtgctac caaccagatt tatcagaatg ctgaggaatt ctatcgtgcg
     2461 gagaatggtc atgcaatcgg ttacttctgg ggttacaaga ctgcgggact cttccagaac
     2521 cagaaggaaa tcaatgattg gatagctgcc ggtaacggta tttatcaggc agatgtgaag
     2581 cctggtgatg taaagtatgt tgatgtgaat catgatggtg ttatcaatgc cagcgataag
     2641 gttgacttgg gaaatggtct tcctaagtat actttcggtt tcaatttcag cctggcatgg
     2701 aagggctttg acctgagtgc taacttcaca ggtgcagccg gtttccagat tgcacagtct
     2761 taccgtgatc ctagctcatc tcaggctaac tacagccgca gaatcttgaa gcgctggaca
     2821 ggtgagggga caagcaatga gattcctcgt gttacctacg gagatgttgg taactggttg
     2881 ttctcagacc tctatttgca ggatggtgac tatatccgct tgcagaacct taccatgggc
     2941 tacgatttca agaagctcat ctcttggaag ggactctcta agatgcgcct ctatttccag
     3001 gtgcagaatc tctttacttt gaccaagtat gatggtatgg atccggaaat cggttcattc
     3061 aacggtacag atggtaacag cagcgattcc tgggtatcag gagttgatat gggttactat
     3121 ccacatcctc gtactttcat cgttggttta aatcttgcat tctaactctt aaacagaaag
     3181 taatatgaat aagaaacata tattgatgag tgccctgatt ctctcttcca tggctttgac
     3241 gtcatgcgat gacttcctgg acacttcatc taagacaaag atgaattccg agactgctta
     3301 cagcacaccg gctgcagcag acatggatct tgtgggctgc tacgatggat ggcagcgtac
     3361 attgagcgat gaaggtgtcg gtatgtacct gacggctgag tttgcctccg agcaggcttt
     3421 cgcaggcttg ggtctttctg atgccaagaa caataatgcc atcgaccagt ttgatttgac
     3481 agttgctcca tcatatacag atctcttcaa tacagattgg aagaattatt atcaggccat
     3541 ctaccgttgc aatcagctga ttcaggctga tgctaccatc aagtggaatg gtgataccaa
     3601 ggcagaaggc cgtatcatgg gcgaggctcg tgcccttcgt ggcatcctct atttcgattt
     3661 ggcccgtttg tttggtgatg tgcctttgtt gctcgaacct tctgaggcta acataccgcg
     3721 tactccggtg aaggaagtat atcagtctat cttcgatgac ttgaagtatg ccgcagccaa
     3781 tattcctgcc gatgcttatc ctttgaagga ccgtgattcc aatgatggcc gcatcaccaa
     3841 gtatgctgct gaggctttgc tggctcgcgc atacctttat tatacgggat attacggcga
     3901 ggagcatcct gcatgttcca aggctgaggc tactgctgcc atcaatgatg tggtagaaaa
     3961 cggtggctat gagttggaga agaactatgc cgatttctgg atgccttcct gtaccaagga
     4021 tgcttcttca acgggtaact atgcctggga aacaacctat gccggcaagt ggtatgacgg
     4081 aaagacctgg aaggcaggac agggtagtct ctctaaggag attgtcttga acctgaagct
     4141 gaactctacc cacgattaca atggcaatgg tgatggtaat accttctcgg tatatttggg
     4201 tcctcgcaac cgttgtgcta ctgatatatg catcgccagt ggttggggag cttgcccggt
     4261 tactccttat ttcgtagagc agtttagaaa cgatcctcgt ttcaatgcct gtgtctggag
     4321 ctgcaaggat gccggtttta cagcagatga gtctgactct tatgagtata cgggttatta
     4381 tacccgcaag tatgctccaa tgtgttttgc cgatggtact cgacaggaag tgggcttcca
     4441 gttgggtgag cagcatcaga acatcactta ctatcaggat tataccatca tgcgttatgc
     4501 tgatgtgctc ttgatgcact ctgagctgaa tggcactgcc gatggtctga acaaggtacg
     4561 tgcccgtgtg ggtcttgaac cagaagacta cagtattgag aatattcgca aggagcgcgc
     4621 catcgagttg gctttcgaag gtgttcactt ctgggatttg atgcgttacg gcaaggatgg
     4681 ttcttatgca gcacaggaaa ttgccaaggc tcagaatggt gccaaggtaa agaatggtgg
     4741 agtggaggca gctaccaagt ttgctgttga caactttacc agcaagaagg gtctgatgca
     4801 gattcctaat actcagatta cgctttccgg taacgttctg actcagaatg ccggatggta
     4861 aagttgggta ggtaatataa taaacattta ataataagaa gatatgaaaa tacataatct
     4921 tatatatacg cttcttgttg ccatggcagt cttcttgttg gcatcttgct caccagaaga
     4981 tttcggattt ggcaagaata catactctcc agaagatttg gtggagggta aggcttatac
     5041 ggttaccata gatggtaaca tcgtgaagct ggaatccaag attcctgatg ccactccgct
     5101 ttggataact cctgtgggac gtaagtccca gcaggctttg gatgtagaat tgccttttgc
     5161 cggcgattat gaggtaacct ttggtgtgga aactcctggt ggtatcgttt atggcgagcc
     5221 ttataagttg agtctttcaa agaacgactt ctctctcttg aacgatgaca agtggttcct
     5281 cctggcagat aagaattaca agacgggcga ggctttccca tctgccgaaa cgctcgctgc
     5341 cggtgtcagc aaaaaatggt atccttgcga tggtaattac aatatcggcc gttgtacgag
     5401 tcctgtgatg tattgctctc cttacgatcc tgactacgac ggtaaaggat atactgctga
     5461 tgaagaggca aatgccgttt ataaggatat cctcttcggt tctgataatt ggaaaccaaa
     5521 ctgggatcct ggcttccaga gctggctgat tccagaaact gatccttaca tggattctta
     5581 catgacattc agcatggatg ctaagaatgg ttgtgtggct accatgtatc gtggtgagtc
     5641 gggtgagaag ggtgccagca caggtagcaa catggtgggc aagttcaatc tgggattggg
     5701 cgacaagacc aagcctacca tcactttcaa cgactgctat gcgatgcata atataggttt
     5761 tgatgcagtt tgctccaact atactcagaa cattcagatt gttgagttga ctccatacct
     5821 ccttcagttg gtgacaaaac gtaccaattc agagggcaac tggtatcttg tatggaactt
     5881 cgtttcagag gatgtgattg ctaccaaggg tgcttgcatt cctaaggcag accaggagct
     5941 gattgagaca gtgaagccaa ttcttcctac tttcgagaat atccataccg acttgttcac
     6001 tgccgatatc aatggtgaca agtatgttgg tagcaagatg actttcaatt tggattctga
     6061 gactccttat gatttcttgt ggtggaatgg ttctcctaat gtgaaggcgt gggagagcgt
     6121 aatcggaggt aagtacaata ctacatgggc tcctgctctt ggcgacaagg caatcgataa
     6181 cttcgagttg actatcagta aggcttctga tggctcttac aattatgaat gtggtgaggt
     6241 agatggtaag gtgaaaatca ccgagaatac catgaccttc gataaggaaa tctctgtatt
     6301 tgctgtggca ggcgataagc gcaccatcga attgaagggc aagaagttct atatccttgg
     6361 caactctacc gatgatcagt atctcactat cggtattcct gagacaacgg atgagaatgg
     6421 cttggtcaac tcctatctcg tagctaagtt ggcttacaag aagattgcta ctggtcctgt
     6481 tggtcctacc aaggtggcta tcgacaattc catcatccac aacgagggta tcatgtgggt
     6541 tgagaatggt tgcctccgca tcggcttcca tcattatggt gaaacaggca agggactctt
     6601 caaggatgtg aagtctgtga agctgaagaa gaaccagacc atcaccgtta ccttcaagat
     6661 taagggtggc gtgacatgga gcaagactcc taagtgtgct ttgattgata ataatatcaa
     6721 gcaaacatgg gaaccaggtg catacgatct tcctgatgct gttgcagtag ataccaatgg
     6781 cggtgagact actgtttcac ttaccaatac caccggttct acacagacct tcacggcaac
     6841 atgtctcgac ctgtcaattc agttggatgg ttttggcgac tacggtggct caaccgacaa
     6901 catcgacata gagattgttt cctgcacaat tcaataactt taatagcgca aacttatgaa
     6961 gaataaaata ttatacggaa ttatggcctt tgtcatgggt atcttcatga caggctgtag
     7021 cgatgatgat tattctatca gcaatactcc actgctcacc gatgagtcgg tgaccacagg
     7081 ttctgctgat gtgaccgtga catccgctac attccatggc acggtaaagg gactcgaaga
     7141 gcagaatgcc agtgcctatg cattaggctt ctactatggt aagagcgaag ataacttgag
     7201 tgagaaggta gctgcttctg gaagcaatga gttccaagct acggtttccg gtatgccagg
     7261 tgatgtggtt tactatcagg cttatgttac cttgcagggt agggtaacct acaagggttc
     7321 tgtgcagagt gccatcatga ccgatgccaa ggctattacc ggcgaaccga aggatttgac
     7381 agccaactct gttatcctga ccggtaagtt ggagaaggct cctcaggagg ctaccagcgg
     7441 tatcgtaatc tctggtgtcg agggctctga gaaggtgcgt gccggtgttc gcatcgtggc
     7501 tgccggcatc aacgacaatt acgaaatcaa ggcagagggt ctgttgccta atacaactta
     7561 ccactatact gcctatctcg acctcggaaa tggtactgta tatggtgaag accgtacctt
     7621 cactactgct ccagctgatt tcaatcctga tactgacctc gttgacttgg gcctttctac
     7681 caagtgggct aagtataatg taggtgctac tgatgagaag cagctgggtg gtctcttcgg
     7741 atttggtgat atgaccggtt tccagacatc tatcaatctg gacgattatg cttctgctga
     7801 tatttacaag accgaccgtg atgtagccaa taaggtatat ggttcatggg ttaccatgcc
     7861 aaccatcgat gagtttgagg aacttttcac agagtgtaag aaagaatggg tagaggatac
     7921 cgagaatcat gtagctggtt acaagtttac cggtcctaat ggtaactcta tcttcttgcc
     7981 tgcagccggt acccgtactc agggcaatgt ttccggcgag ggtttgaacg gttactatct
     8041 ctctggttca atcaatgcca cagacaacag atttgccatg gcatacaact tcgaccagaa
     8101 tgctgcacgc cgtactacca ctcctgttta tcaggcattg gctatccgtc cggtttctgt
     8161 agcaaagaat gtgaagtttg acaaggctaa gctgattggt cagacttggg aaatcgacct
     8221 ccgtctggat ggttctcact ataagtttga tggtcctgtt tacttccttg gtaaggacga
     8281 cagttgggct accattacta ataatcagcc tgttgtaggt aatgttgggt gctgggctgc
     8341 tgatttcgca agcaactctt ggacagtagg tggtgatgcc aagaactgcc agggtactat
     8401 gaccttcaat gaggatggta ccgttaaggt tcatcagtat gtggctggtg ccgatggtgc
     8461 tgagggaaca ttccaggata ccgagggaac ctataccatt gatgagaaaa acaagaccgt
     8521 gaagttgagc attgctcctt tgatacctgc taactatatc ggtaatgtga aggagaccaa
     8581 ggatatggat atccgcatct tgtctttgac tgatgagacc atgcagttgg ctgttactcg
     8641 tgcaaacgat aatcagtata tgagtatcaa ctatgtacca ggcgagttga agaatggctt
     8701 tactgctaag cttacctgct acggtggtga tgatgagaat acacctgatg cctggaattc
     8761 tgctaccgtt aatgtattgg gtggcgctgc aggcttgaag acttacaccg taaccttcaa
     8821 tacccagtat cctcgtacca acggtaaggt ttatctcctc gaattggagg gatactctgc
     8881 tgcttatcct aaggcactgg ttcgcatcga tgcaatcaag gctgatggta acgacgtgaa
     8941 gtttgatgcc aacaagttct tctatggcga cctggaaggc aatggcaact accgtgtaga
     9001 aatggctaac atctggggat gtggtcataa tgacagctgg gacggtttga aggatactcc
     9061 attccataag gatggaggcg agacaaagaa tgagactgcg cttgcattca accatacttt
     9121 cgaggtaacc tttaccgttg tttccaacac aagtgatggt actggtgtct acactcctac
     9181 attcaatgcc gttcgtggct ggggtgaagg tgaagctcag ttgtttggat ataacgatgg
     9241 ctctactttg aaagttgtta agagtgacaa gggacaatac tctctcgaga acaatcagtt
     9301 cgatatgact tatgaaggtt cgggctttga aggtggtact atcatgacat tcgttgagat
     9361 tgctgacctt tatggtttct tcccaggcac acacagtact ttggatgagt tctatcttga
     9421 cggtaaggcc gtttcttacg ataagtctaa ggtggttgac gccaatgaaa atcctaagta
     9481 tcgtttggaa ttgtttaatt gctatgctgc tacaaaggat aattgtgcat ttggtgtcaa
     9541 ggatggtgat ttgatgcgag agctgggctt taataagtct atgcgcgcta agtttacagt
     9601 tcacagtctt ttcgctgtcc cacaatggta atggttgata aataataata cagagaatcc
     9661 gatagttttc agaacatatc gggttctctc taaaatgaaa agaaaatgaa gaaattcaaa
     9721 tatctatatt tgttgctctt gggcatagtc tgcggattgg ctttctcggc ttgctcttac
     9781 gaagacgatg attatgatga gcctagcttc aaggttctca acccagagct ttcatttgac
     9841 ggtactggcg gagtgcagac catcaatgtt caggcagatg ctcagcctac agcttccgtg
     9901 atagaaggtg ccgactggtg ctctgttgct tataaggatc aggctgccgg cacatacaat
     9961 ttcgatgtaa ccgtagccgc ttctcaggaa gatgaagtaa ctactgctac cgtccgtatc
    10021 attcagggct atagccgtaa ggatgttacg attacccgtg ccaagaaggg ggctgttgtt
    10081 actcctgatg taccaccagc tgatatgaac aagactgcca tggaggtggc tcagttgatg
    10141 tatccaggtt ggaacctcgg taacaccctg gaaggtggcg acagcaagaa tctctggaag
    10201 aatgctggta tcgaaacaga gacagtctgg cagaatgcca agaccactca ggctttgatt
    10261 gatgcagtga aggctgctgg tttcaagagt gtccgtattc cttgttcttg ggtgatggga
    10321 catattactg atgctgaaaa atgcaccatc gaccctgctt ggatgaagcg agtaaaagag
    10381 gtggttgatt actgtatcaa ggatggtctc tatgttatca tcaaccagca ttgggatggt
    10441 ggatggatag agcacaatgg tatgacagcc aatgcagaca tcaagactac caaggctcag
    10501 ctcactaaaa tatggactca gattgccgat aatttcaaga catatgatga gcatcttctc
    10561 tttgccggca tgaatgagcc aggtgtagga gcaggtgaag gtgatatcat tggtgtggct
    10621 gatatgagta accgtattgc tgaatatgag cagactttca tcgaggcggt tcgtgcaact
    10681 ggtggcaaca atgccaagag agttctgata gttcagggtc ctaataccga tatcgataaa
    10741 ttcgtagcca acaactacat gtctaagatc catgattctg ctaccgaccg attgatggta
    10801 gaggttcact tctatgatcc atatcagttt actgatttga gtgaagacaa ggattggggc
    10861 aagtactatc tctattgggg caagaacaat accaatggtt ctgaggctgg tcgtacagct
    10921 gatgctaaat ataacgagga ttatgtagag gctcagatga agaagatgaa gaccaacttc
    10981 ttcgacaagg gttatccagt gttgattggt gagtttggtg ccaaccagcg tttggccatc
    11041 ggtaaggatg ctgttcatga tgcatcagtt aaggactatt acaaggctgt tgtaactagt
    11101 gctatcaata atggttgtgt gccaatggca tgggatacca atggtggtct tcctagcatg
    11161 accatcttca accgtgctgg tgcaagtgtt agcaatacta atatgcttga gagtattaca
    11221 gccggagttg cagcagcaaa gtggcctgct aaataaaaaa cgagatatta agtctttagg
    11281 tttgatataa agtaggtgtg ggcgcatatc aggggattct ttctctgatg tgcgtccttt
    11341 ttttgtgcaa atatcgtaca tcctaacata atatacctat ccttttatca aaagtataca
    11401 ttcacgctac tgttttttcg ctatctttgc aacatgataa cttcaaaacc cgataatacg
    11461 ttagattgat atatgaagaa gatagttatg attatattgg ctgctgctca tttcgcttgg
    11521 gctggcgcac aggttaaatc aatggatggt cgtatctcga aaatgaaact cacggctacc
    11581 gaactggctc actatatggc tccaggagtc aacctgggta atacgatgga ggcgtgcgac
    11641 tggaatgatg tctttaccaa tcaggcagga ctgaagtcag aaacttcatg gcagaatgat
    11701 aagactactg agagctatat ccggagtttg aaacagcagg gattcaacag tcttcgcatc
    11761 cctacatcct gggtggctgg gcatcttacg gataaggaga atatgaccat agatcctgtc
    11821 tggatgaaac gaatcaagga aatcgtaaac tatggtctca atgccggtct ctgtgtcatc
    11881 atcaacgaac actgggatgg tggatggatg gagcatgatg cctttaccag tggtgcgaat
    11941 gtgacagaat ataaggagat gttccgcaag ctgtggacga atatagcaaa ggaattcaag
    12001 acgtatgacc agcgggttct ctttgctgcc ctcaacgaac cgggagtggg aggcgccagt
    12061 ccgcaggttc aaggcgacat gctggctcct gatagtaagg agtttgccga tcgtctcctg
    12121 gcatacgaac aggtgtttat cgatgccgtc cgtgctaccg gtggtaataa ttccagccgt
    12181 gtgctcatcg tacagactcc aaagaccgag attgatttgg cagccaagga ttcttacgac
    12241 atcacccgtc tgaaggataa ggtgaagaac cgtctgatgg cagaggttca tttctacgat
    12301 ccttatatat ttaccctgat ggacaaggat gccgattggg gcaaggtggc tctttattgg
    12361 aaaggacatg ctcctgccga tgatcaggga cgaaccgtaa acaccatctg gtataataat
    12421 aagaatgtgg atgcctacca gcatatcacc aatcaggtga tgaagatgaa acagaagttt
    12481 gtagatctgg gctatccagt agtcatcggt gaatatggtg ccaaccgtaa ggatgcctct
    12541 ctcttcggtg gtaatcagga gaagcataat gagagtatga tggcatggta tggtgctgtg
    12601 actgccgaaa tgatgaaggc aggacttatc ccatacgtct gggatatcaa tgtgcagcct
    12661 cttccccaca tgaccatctt cgacagaaag aagcaggtag tttccgactc ctatatattt
    12721 aaaggtgtga tgcagggagc agccgaaggg atggaagatt acctgagaat ctatccgaaa
    12781 ccttagaaaa atagagaaaa agcaataata aataagcata gttttgattt atatgtagaa
    12841 agggcaaaaa agtatcatgc tttcttgccc ttatttagta tctttgcagc cgaaaatgaa
    12901 tctttaaact gtaaacttaa taaataaatg aagaagatat ttgttttttt gatgatggca
    12961 ttggctacat gcaatatctc tgcgaagtca aattttgtgc aggtaaagga cggacatttc
    13021 gttcgcgacg gtaaacctta ttattatgta ggtaccaatt tctggtatgg tgccatcctc
    13081 ggttcagaag gacagggcgg caaccgtgag cgtctctgca aggaactcga taagatgaag
    13141 gagatgggct ttgacaacct ccgcatcctg gtgggctctg atggaaagcg aggagtgaag
    13201 accaaggttg agccgacgct gcaggaagct ccgggcgttt ataatgatac gattcttgcc
    13261 ggtctcgact atctgctgat ggagatgggc aaacgcaaga tgctggccgt gctctatctg
    13321 aacaattcct gggaatggag tggcggttat ggctattacc tggagcaggc tggtttgggc
    13381 caggctcccc gtcctaacga agatggatat cctgccttca tgaacttcgt cgccaaatat
    13441 gcatcctgcg aaaaggcgca tcagctattc tatgattatg tgaaattcat acttacgaga
    13501 accaacagat atacaaagaa aaagtataag gacgacccgg ctatcatgtc atggcagata
    13561 ggtaacgagc ctcgagcttt cagcaaagag cagttgcctg ctttcgaaaa gtggctgggt
    13621 gaagcgggca agctgatccg ttcgctcgat aagaatcatc tgatttctat cggtagcgaa
    13681 ggtaaatggg gatgtgaagg tgaactgaac tgctgggagc ggatctgtgc cgacaagaat
    13741 gtagattatt gcaacattca tctttggcca tacaactgga gttgggcgag aaaagaccat
    13801 ttgcaggaag atttgggaat ctcctgcaag aacaccaagg agtatattga tgagcatctt
    13861 gaggaatgcg gacgtatcag gaagccattg gtgatggagg agttcggcta tcctcgtgat
    13921 ggtttctctt tttcaacttc ttctacaaca gaagcacgtg atggatatta taagtatgtg
    13981 ttcagtcttg ttggcgacaa tgctgcatct ggcggttact ttgccggttg caacttctgg
    14041 ggatggggcg gctttgccaa tcctaagcat gagcagtggc aggtaggtga tgactacacc
    14101 ggtgatcctg ctcaggaagc gcaaggtctg aactccgttt tttctactga taaatctaca
    14161 ttggatgtag taaagacgca ggtagacaga atgaagaaga ttggtaagtt ataa
//
