LOCUS       NZ_JH815204             8254 bp    DNA     linear   CON 08-JAN-2025
DEFINITION  Barnesiella intestinihominis YIT 11860 supercont1.2, whole genome
            shotgun sequence.
ACCESSION   NZ_JH815204 REGION: 771118..779371
VERSION     NZ_JH815204.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02595395
            Assembly: GCF_000296465.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Barnesiella intestinihominis YIT 11860
  ORGANISM  Barnesiella intestinihominis YIT 11860
            Bacteria; Pseudomonadati; Bacteroidota; Bacteroidia; Bacteroidales;
            Barnesiellaceae; Barnesiella.
REFERENCE   1  (bases 1 to 8254)
  AUTHORS   Earl,A., Ward,D., Feldgarden,M., Gevers,D., Morotomi,M., Walker,B.,
            Young,S.K., Zeng,Q., Gargeya,S., Fitzgerald,M., Haas,B.,
            Abouelleil,A., Alvarado,L., Arachchi,H.M., Berlin,A.M.,
            Chapman,S.B., Goldberg,J., Griggs,A., Gujja,S., Hansen,M.,
            Howarth,C., Imamovic,A., Larimer,J., McCowen,C., Montmayeur,A.,
            Murphy,C., Neiman,D., Pearson,M., Priest,M., Roberts,A., Saif,S.,
            Shea,T., Sisk,P., Sykes,S., Wortman,J., Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform
  TITLE     The Genome Sequence of Barnesiella intestinihominis YIT 11860
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8254)
  AUTHORS   Earl,A., Ward,D., Feldgarden,M., Gevers,D., Morotomi,M., Walker,B.,
            Young,S.K., Zeng,Q., Gargeya,S., Fitzgerald,M., Haas,B.,
            Abouelleil,A., Alvarado,L., Arachchi,H.M., Berlin,A.M.,
            Chapman,S.B., Goldberg,J., Griggs,A., Gujja,S., Hansen,M.,
            Howarth,C., Imamovic,A., Larimer,J., McCowen,C., Montmayeur,A.,
            Murphy,C., Neiman,D., Pearson,M., Priest,M., Roberts,A., Saif,S.,
            Shea,T., Sisk,P., Sykes,S., Wortman,J., Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2012) Broad Institute of MIT and Harvard, 7
            Cambridge Center, Cambridge, MA 02142, USA
COMMENT     REFSEQ INFORMATION: The reference sequence is identical to
            JH815204.1.
            This is a reference genome for the Human Microbiome Project. This
            project is co-owned with the Human Microbiome Project DACC.
            Source DNA provided by Morotomi, M. (Yakult Central Institute for
            Microbiological Research, Japan).
            Bacteria provided by Morotomi, M. (Yakult Central Institute for
            Microbiological Research, Japan).
            Please be aware that the annotation is done automatically with
            little or no manual curation.
            Gene Calling Method: Prodigal.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Finishing Goal           :: High-Quality Draft
            Current Finishing Status :: High-Quality Draft
            Assembly Method          :: allpaths v. R42667
            Assembly Name            :: Barn_inte_YIT_11860_V1
            Genome Coverage          :: 130x
            Sequencing Technology    :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000296465.1-RS_2025_01_08
            Annotation Date                   :: 01/08/2025 17:37:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,797
            CDSs (total)                      :: 2,722
            Genes (coding)                    :: 2,696
            CDSs (with protein)               :: 2,696
            Genes (RNA)                       :: 75
            rRNAs                             :: 4, 4, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4 (5S, 16S)
            partial rRNAs                     :: 6 (23S)
            tRNAs                             :: 59
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 8 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8254
                     /organism="Barnesiella intestinihominis YIT 11860"
                     /mol_type="genomic DNA"
                     /submitter_seqid="supercont1.2"
                     /strain="YIT 11860"
                     /type_material="type strain of Barnesiella
                     intestinihominis"
                     /db_xref="HMP:9448"
                     /db_xref="taxon:742726"
     gene            complement(1..3051)
                     /locus_tag="HMPREF9448_RS08020"
                     /old_locus_tag="HMPREF9448_01639"
                     /db_xref="GeneID:77848888"
     CDS             complement(1..3051)
                     /locus_tag="HMPREF9448_RS08020"
                     /old_locus_tag="HMPREF9448_01639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862098.1"
                     /GO_component="GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="WP_008862098.1"
                     /db_xref="GeneID:77848888"
                     /translation="MKKFTVCGAAVALCCLAGNVQAQEPEYPASASTEVFDFTPWNDD
                     KLLLLFAQAADEGRKYPTKEEFEAAGFNLDLEFSRSHVRPAVIMEDAAKNIVADVYPT
                     RRLWMNTPTGQGESVGGYPSSEFHSDVFSMWNYTNLYGAWNHTILQAPGSWADAAHKN
                     GTHMFSGIKFFESWGTTSSEYIKLITKKNEKGEYVYVDAFLNALLFLGLDGINYNFED
                     SGYQQTDVVGFHQALYKRAKEIGFDSFHIGLYTSSSSLSTRTANALYGTKANGKTADL
                     MLNYSGGDFATQYMASSVQAAETAYGTADGLYAGGWYRHMDLSWPLLNQDEATKRCGL
                     CLWGEHKISRFFQYVVGKDPMDMQTNYQKLLEKGFSGGYRTPIQRPAPSYTNVFQVEN
                     ASEADTQLAEFCGLAEFIPERTAIQGDLPFNTYFSLGNGDFYAYKGKKTLGSWYNMGQ
                     QDYVPTYRWLIYQTGTTNRSTDIDAEFTHEDAYIGGSALRLKGTPTSTGSDVVLYRAK
                     LTVSGNNATAKLAVKSGIEGSNASNLYLILKKFDDNAWLEFPVGDLKGATWEEKEIAL
                     SGINQNDVIEYIGLRVKGSASDSYKMLVGQLQLCDDRSAVKPADIDDLSVVTEVKEET
                     TSSMSVKLTWSVDYTGFSPARKDYGMVYNDEVNIDHFEILYKNGEDGRVSEVGRTSTW
                     SAYVGNIQFEDENDDPYIGVRSVSVDLKSYSPVVWTHVPRATGSLPEPDTDLYGKTYL
                     NLESEGVETAQKYRYVSSLVTTGATENLNYTSNTPVGGDNYLRVTDQKLKVEQGQKVT
                     VKFAGRVNGSDDGLKWCLGKAYVDWNLDYVFDASTDELVMEVGTLNAGTDSIATGCTF
                     ILNVPEDAAVGTSRFRMVFSDAWFTHPGPTGGTAKGFSIDFDVEVTGSNSGREPEKTY
                     ADYRDQGVADEPDGLDVSGIEDVVNGNGNGVSSVSSLYPTVATDVIYFNNVDKAWIYT
                     VDGQLVKYVNNNPESVNVSDLSSAMYVVKMQKGNVVRSQKMFKK"
     gene            complement(3143..4930)
                     /locus_tag="HMPREF9448_RS08025"
                     /old_locus_tag="HMPREF9448_01640"
                     /db_xref="GeneID:77848889"
     CDS             complement(3143..4930)
                     /locus_tag="HMPREF9448_RS08025"
                     /old_locus_tag="HMPREF9448_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="WP_008862099.1"
                     /db_xref="GeneID:77848889"
                     /translation="MKKTVLAAFGALLSVVSLHSCLDFDMPTDTFTGGDTELDPIVYK
                     GKADSIDYRKEISEEGFAFAEKELNNYFFQLQTVQYCMRGGKEGQLPGAHQYQYVYNI
                     TVDNYAGYLCVPHNFSHGGDGILSSTYYYNRGYCDGPYGAFLMVKNNLVNMLNHPHID
                     SIPEMKAIGLLLYNYSAQEMVDLYGSIPYVDHKNNKETNPFTFNKMADIYETIVDNLD
                     TINACLKHYEQRPGWYKSRLNGILQQSDWVTKDFSIDTWRRFANSLKLRMAMHIVKVD
                     RTKAQKWAQEAVTEGVIETENQEIALDPVVSGFTHPLVDISKLWGDSRLNASFESLLF
                     SLKHPYVQDDYALFEKNSSQIVNETNPEEIEPTNSRIVGLRAGISMVSGQTVSVNPRI
                     AYSAVSSNRISMAPLYLMKLSEVQFLRAEGAVRNWAMGGSAQSFYEAGIRSAQLDQRT
                     NSQYGQKVDEYMSVEEPVAYAYTDPMDHTNDIASVTKIGVKWNEGDTPETKLEKIITQ
                     KYIAGFPYSYEAWNDLRRTGYPKIFPVLNVEDGDGSLVQGDLIRKIPLPGRDTSAGLS
                     DIQTSGIEALGGTDTQGTRVWWDVEGSNF"
     gene            complement(4952..8254)
                     /locus_tag="HMPREF9448_RS08030"
                     /old_locus_tag="HMPREF9448_01641"
                     /db_xref="GeneID:77848890"
     CDS             complement(4952..8254)
                     /locus_tag="HMPREF9448_RS08030"
                     /old_locus_tag="HMPREF9448_01641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862100.1"
                     /GO_component="GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="WP_008862100.1"
                     /db_xref="GeneID:77848890"
                     /translation="MYRRNLKAIIVAFVLVSGFGIGTAAHVSAADTMSPQGAAQSKSV
                     NQNVATGTKVVTGTVTDPSGETLIGLTVKVSGTNVAVATDLDGNYSIKVPAGSNELEF
                     SYIGYQTIKRAINGQSKIDVVMQDNSEVLDEVVVTAMGITRKESSLTYATQEIKGDDL
                     MKVQDANFVNSLQGKAAGVTITQSAGGAGGTSKILLRGNKSVMGNNSPLIVIDGIPMT
                     NQVGGAASSWAGGESLTYSSSSEGGDALSLINPDDIESINILKGANAAALYGSAAANG
                     VLMITTKKGKEGRVSVSVNSNVTFENPLVLPQIQNVYGANVNLAASTLTTESWGKKIS
                     EMTPEELNYSGAKLRNYAKDDISDFFETGATYNNSVSVSGGTEKVRSYFSYANSYADG
                     MVPNNTYQRNTFSFRQSYSLFNKKLNVDLSLNYVHAQTKNRPGGGTVMNPLYDLYRMP
                     RNIDMDYYKKNYRGEGTWTSNIYGYYNDQKQWVPDGTIELSGPMQQWAYFSPGNNNPY
                     WITNANKGQTEEERAYGYITASYEIIPGLKIQGRLNMDRAKYKGFTKRMATTQNVAAI
                     EDYGMYGQDLIWSNDVYVDAMLSYNKEIKDFSVSASAGWVGHTVKGETQKLWTRATYF
                     SYTDMNQLPTRINFFEPLASWGGSNMNEYSLSSNWDKGLFFTGQVGYKDYVYLEGSYR
                     QDWYRAFKQFEYRGTPDNYGYFSVGANTLMHRYISLPEFITHLKLRASYSEVGNSIPN
                     EVFNKGKADLATGAIASSTYGYFDNPIPETSKSFEAGFDVSFFDSSLNWDLTYYHTGL
                     YNNYFLQATTGGKSKPVNTGLIRNQGIETTVSYSLGFAKDWMWRTSVNFSYNDNKIVK
                     TFKDEQGKSSKLEQKIAGGNIVVRYDEGGSYGDMYALDFRRNDDGSIYLSSNGAPQLS
                     NNSYVYLGNMNSKFQLGWGNTISWKDLSLYFLVSGRIGGKVISLTEAYLDYQGVSKRV
                     GDARLAAEANPDLQWNGKPAMVMPDGNLAPIEEYYKAVGGNMFGSQYVYDATNFRLAE
                     LSLGYSFHNLFGGVISNLSLSVVGRNLFFIYKDAPVDPDIALSTKNGLGAFDIFNMPA
                     TRSVGVNLKIDF"
ORIGIN      
        1 ttatttcttg aacatcttct ggctacgtac cacattgcct ttctgcatct tcactacata
       61 catagcagaa gacagatcgg agacattcac agattcggga ttgttgttta catatttaac
      121 caactgtcca tcgaccgtat atatccaagc cttatctaca ttgttgaaat agattacatc
      181 ggtagctacc gtaggataca gcgaagaaac agacgataca ccgtttccgt tgccatttac
      241 gacatcttcg attccagata catctaatcc atcgggttca tcggcaacac cttgatcacg
      301 gtagtcggca tatgtttttt ccggttcacg tccggaattc gatccggtta cttccacatc
      361 gaaatcgatc gagaatcctt ttgccgtacc tcccgtaggt ccggggtgag taaaccaagc
      421 atcagagaat accatacgga aacgacttgt acccactgcc gcatcctcgg gaacattaag
      481 aatgaaggta caacctgttg cgatagaatc ggttcctgca tttaacgtac ctacctccat
      541 tactaattcg tctgtactag catcgaatac ataatcgaga ttccaatcta cataggcttt
      601 acccaaacac cacttcaaac catcgtcgct accgttaact cgtcctgcaa acttaaccgt
      661 tactttctgg ccttgttcta ctttcagttt ttggtcggtt actctcaaat agttatcacc
      721 tccaacaggt gtattcgatg tatagttcaa attttcagtt gcacccgtag taacaagcga
      781 tgaaacataa cgatattttt gagctgtctc aacaccttcc gattcgagat tcaaataagt
      841 ttttccatac aaatcggtat cgggctcggg taaagacccc gtggcacgag gtacatgcgt
      901 ccacaccacc ggagaatacg atttcaaatc gactgatacc gaacgaacac cgatataggg
      961 atcgtcgttt tcatcttcaa attgaatatt acctacataa gccgaccaag tggaagtacg
     1021 tcctacttcc gatactcgtc cgtcttctcc attcttatac aaaatttcaa aatgatcgat
     1081 atttacctcg tcgttgtaaa ccataccata atctttgcgg gcaggactaa atccagtata
     1141 atcgacactc caagtcaatt ttacagacat cgaagaagtc gtttcttcct tcacttctgt
     1201 aactacactc aaatcgtcga tatcagcggg tttcacagca gagcggtcat cgcataattg
     1261 caactgtcct acgagcattt tatagctgtc cgatgccgaa cctttcacac gcagaccgat
     1321 atattcgatt acatcgtttt ggttgatacc gctgagagct atttcttttt cttcccaagt
     1381 agcgcctttc aagtcgccca cagggaattc caaccatgca ttgtcgtcaa atttcttcaa
     1441 gatgagatac agattggaag cattacttcc ctcgataccc gatttcacgg ccagtttggc
     1501 tgttgcattg ttcccagaaa cagtcagttt ggcacgataa agcactacat cagatcccgt
     1561 cgaagtagga gttcctttca aacggagggc agatccaccg atataagcat cctcatgagt
     1621 aaattcagca tcgatatccg tcgaacggtt cgttgtcccc gtttgataga ttaaccaacg
     1681 ataggtcggt acatagtctt gttgtcccat attgtaccag ctacccagtg tttttttacc
     1741 tttataagca tagaaatctc catttcccaa tgagaaatag gtattgaacg gcagatcgcc
     1801 ttgaatagcc gtacgttccg gtataaattc ggccaatccg cagaattcgg ctaattgcgt
     1861 atctgcttca ctggcatttt ctacttggaa tacattagta tacgaaggag ccggacgttg
     1921 gatcggtgtg cgataccccc ccgagaatcc tttttccaac aacttttgat agttcgtttg
     1981 catatccatg gggtcttttc ccactacata ttggaagaaa cgactgattt tgtgctctcc
     2041 ccaaaggcaa agaccacatc tcttcgtcgc ttcatcttga ttcaagagag gccagctaag
     2101 atccatgtga cgataccaac caccggcata taaaccgtcg gccgtaccgt aagcagtttc
     2161 tgccgcttga accgacgaag ccatatattg tgttgcaaaa tctcctcccg aataattcaa
     2221 cattaagtca gcggttttac cgttcgcttt tgttccataa agggcatttg cagtacgtgt
     2281 cgagagtgaa gaactactgg tgtacaaacc aatatggaag ctgtcgaacc ctatctcttt
     2341 cgctcttttg taaagcgctt gatggaatcc gacaacgtca gtctgctgat agcccgaatc
     2401 ctcgaaattg tagtttatac cgtccaatcc caagaacaaa agagcattca aaaaggcatc
     2461 tacatacacg tattctcctt tctcgttttt ctttgtaata agcttgatat actcacttga
     2521 agttgttccc caagactcga aaaacttaat acccgagaac atgtgcgtcc cattcttgtg
     2581 agcggcatcg gcccaagaac ccggagcttg taatatagtg tggttccaag caccatataa
     2641 attcgtatag ttccacatag agaatacatc gctatggaat tcggagctgg gatagccacc
     2701 gacactttca ccttgtccgg tcggtgtgtt catccacaac cgacgggtgg gatacacatc
     2761 ggcaacaatg tttttggctg catcttccat aataactgcc gggcgtacgt gcgaacggga
     2821 gaattccaga tcgagattaa aaccggcagc ttcaaactct tctttggtcg ggtacttacg
     2881 tccttcatca gccgcctgag cgaataacag caataacttg tcatcattcc acggtgtaaa
     2941 gtcaaaaact tctgtcgatg cagacgccgg atattccggt tcttgcgcct gcacgttgcc
     3001 ggccagacag catagagcca cagccgcacc gcatacagta aacttcttca tgataaaaaa
     3061 acattaaatt ataaattttg taactattat aaatagtaga gagaagagga gcgacagctc
     3121 ccctgccctc taaaccattt cattagaaat tggaaccttc tacgtcccac caaacacgag
     3181 taccttgtgt atcggtcccc cccaaagcct ctataccaga ggtctgaata tcggacaaac
     3241 ctgcactcgt gtcccttccc ggcaaaggaa ttttccgaat aaggtcgcct tgtaccaaac
     3301 tgccgtcacc gtcctcgaca ttcaatacgg ggaatatctt cggatagccc gtacggcgca
     3361 aatcgttcca tgcttcatag gaatagggga acccggcaat atacttctga gtgatgattt
     3421 tttccaattt ggtttccggc gtatcgcctt cgttccattt aacaccgatt ttggttacac
     3481 tggctatatc attggtatgg tccatcgggt cggtatacgc ataagcgacc ggttcttcca
     3541 cactcatata ctcatcgact ttctgtccat attgcgaatt tgtacgttga tccaactgag
     3601 cgctacgaat gcctgcttca taaaaagatt gggcagaacc acccatcgcc cagttacgca
     3661 ctgcaccttc cgcacgaagg aactgaactt ctgaaagttt catcaggtat aacggagcca
     3721 tactgatacg attggaagaa actgccgaat aagcgatacg ggggttaaca ctcactgttt
     3781 gacccgaaac catggatata ccagctcgta gccctacaat ccgagaattg gtaggttcta
     3841 tttcctcagg atttgtttca ttgacaatct gagaagaatt tttctcgaac aacgcataat
     3901 cgtcttgtac ataaggatgc ttcaaactga acagtaagct ctcaaacgaa gcattcaacc
     3961 gggaatcgcc ccataatttg ctaatatcga ccaatggatg ggtaaaccct gaaacgactg
     4021 gatccaaagc tatttcttga ttttccgttt cgattacacc ttcggtaaca gcttcttgtg
     4081 cccatttttg agcttttgtc cgatcaactt tcacaatatg catagccata cgaagtttta
     4141 acgaattggc aaaacgtctc catgtgtcaa tactaaaatc tttcgttacc cagtcacttt
     4201 gttgcaagat accattcaaa cgacttttgt accaccccgg acgttgctca taatgtttca
     4261 aacaagcatt gatcgtatcc agattatcta cgatagtttc ataaatatcg gccatcttat
     4321 tgaaggtaaa tggattcgtt tccttattat tcttgtgatc tacataggga atagagccat
     4381 acaggtcgac catctcctgt gccgaataat tgtaaagcaa tagtcctatc gctttcatct
     4441 cggggataga gtcgatatga gggtgattca acatgttcac caaattgttt ttcaccatga
     4501 gaaaagcgcc ataagggcca tcgcaatatc ctcgattgta atagtaagta gaagataaaa
     4561 taccgtctcc cccatgtgag aagttatgag gaacgcataa atagccggca tagttatcga
     4621 cggttatatt atacacatat tgatattggt gagctccggg aagctggcct tcttttcctc
     4681 ctctcataca atattgtacc gtttgtaatt gaaagaaata attgtttaat tctttctcgg
     4741 caaacgcaaa accttcttct gaaatctctt ttctataatc tatactatcg gctttacctt
     4801 tgtagactat cggatccaat tcggtatcac caccagtaaa ggtatcggtg ggcatatcga
     4861 aatctaaaca tgagtgcaag ctcacaacag aaagcaatgc accaaatgcc gctaaaacag
     4921 tttttttcat atatctattc attatttact actaaaaatc aattttcaaa ttcacaccga
     4981 ccgaacgggt tgccggcata ttgaagatat cgaacgcacc caaaccgttt ttggtagaga
     5041 gggcgatatc gggatcgacc ggagcatcct tatagatgaa gaacaagttg cggcctacga
     5101 ctgacagcga gaggttactg ataactcctc caaaaagatt atggaaagaa taacctaacg
     5161 aaagttctgc cagacggaaa ttggtggcat cataaacata ttgcgaacca aacatattac
     5221 cacctactgc tttgtaatat tcttcaatag gagccaaatt accgtctggc attaccatag
     5281 ccggctttcc gttccattgt aaatcgggat ttgcttcggc tgccaaacga gcatctccca
     5341 ctcgcttaga tacaccttga taatcgagat aagcctcggt caacgagatg accttgccac
     5401 cgatacgacc gctcaccaag aaataaagag aaaggtcttt ccaagaaatc gtatttcccc
     5461 atcccaattg gaatttagaa ttcatattgc ccaagtagac ataactattg ttacttaact
     5521 gaggtgctcc gttagaactt aaataaatac taccatcgtc attgcgacga aaatcgaggg
     5581 catacatatc gccgtaagaa ccgccttcgt cgtaccttac cacaatatta ccaccggcaa
     5641 ttttttgttc gagcttagac gactttcctt gctcatcttt gaatgtcttt acaatcttgt
     5701 tgtcgttgta cgagaaattc accgaagtac gccacatcca atctttggca aagcccaacg
     5761 aataggaaac ggtggtttcg ataccttgat tacgaatcaa accggtattt acaggtttgg
     5821 acttacctcc cgtagtggct tgtaggaaat agttattata caatcccgtg tgataatacg
     5881 tcaaatccca gttcaacgaa ctatcgaaga acgatacatc gaatccggct tcgaatgatt
     5941 ttgaagtttc gggaatagga ttatcgaaat agccataggt agacgaagcg atcgctccgg
     6001 ttgctaaatc agctttacct ttattaaaca cctcattagg aatagagtta cctacttcgg
     6061 agtaagaagc acggagtttc aagtgtgtaa taaattcggg caaagaaata tagcgatgca
     6121 ttaacgtatt ggctccgacc gagaaatagc cataattatc gggagtaccc cgatattcga
     6181 attgtttgaa agcccgatac cagtcttggc gatagctacc ttccaaatag acataatctt
     6241 tgtatcctac ttgtcccgta aagaaaagac ctttatccca gttggaagac aaggaatact
     6301 cattcatatt gcttcctccc catgacgcca gaggctcaaa gaagttaata cgggtcggta
     6361 gctgattcat gtcggtatag gagaaatagg tggctctcgt ccacaacttt tgggtctctc
     6421 ctttaacggt gtgtcccacc caaccggccg atgccgatac agagaaatct ttgatttcct
     6481 tgttgtaact caacatggca tcgacataga catcgttgct ccaaatgaga tcctgtccat
     6541 acataccata gtcctcgata gcggctacgt tttgcgtagt ggccattctc tttgtaaatc
     6601 ctttatactt ggcacggtcc atgttcaaac ggccttgtat cttcaagccg ggaatgattt
     6661 catagcttgc cgtaatatat ccataggctc tttcctcctc ggtctgtcct ttattggcat
     6721 tcgttatcca atagggatta ttgttacccg gtgaaaaata ggcccattgt tgcatcggtc
     6781 cagacaactc aatagtacca tcgggaaccc actgtttctg atcattataa tatccgtaaa
     6841 tgtttgaagt ccacgtgcct tctcctcgat aatttttctt ataataatcc atatcgatgt
     6901 tacgcggcat acgatacaaa tcatataacg ggttcatgac cgtacctccg cccggacgat
     6961 tctttgtctg cgcatgcaca tagttcaatg acaaatcgac attcaacttc ttgttgaaca
     7021 aagaataact ctgacggaat gaaaaagtat ttcgttgata agtattattg ggaaccatac
     7081 cgtcggcgta ggagttggca taagagaaat aggaacgtac tttctcagta ccaccactta
     7141 ccgataccga gttgttgtaa gtcgcacctg tctcgaaaaa gtcggaaata tcgtcttttg
     7201 cataattacg caacttagct ccactgtaat tgagctcctc gggtgtcatt tcagaaatct
     7261 ttttgcccca actttcagta gtaagagtac tggctgccag atttacattt gcaccatata
     7321 cattctgtat ctgaggaagt accaacggat tttcaaaagt cacattagaa ttgacactta
     7381 ccgatacacg accctcttta cctttcttgg tcgtaatcat aagcacgccg ttggccgcag
     7441 cactaccgta aagagcagcc gcattagcac ccttcaaaat gttgatgctc tcgatatcgt
     7501 cgggattaat caaggaaaga gcatccccac cctccgaaga actcgaataa gtgaggctct
     7561 cgccaccagc ccaagaagaa gccgctccac cgacttggtt agtcataggt ataccgtcga
     7621 taacgatcaa ggggctgttg ttacccataa ccgacttatt accacgcaaa aggatttttg
     7681 aagtaccacc ggctccaccg gcactttgag tgatagttac accggctgct ttcccttgaa
     7741 gcgaattcac gaagttggca tcttgcacct tcattaaatc atctccctta atttcttggg
     7801 tcgcataggt caaagacgat tctttacggg tgatacccat ggcggttaca acgacttcgt
     7861 cgagcacctc ggaattgtcc tgcataacta catctatctt gctctgcccg ttgatagcgc
     7921 gcttgatggt ttgataaccg atgtaggaaa actccaattc gttactcccc gcaggaacct
     7981 tgatcgagta gttaccatcg agatctgtcg ctacggccac atttgtaccg gaaactttta
     8041 ccgtgaggcc gataagagtc tctcccgacg gatcggtaac tgtacccgtt accacttttg
     8101 tccccgtggc aacattttga tttacactct tcgattgtgc cgctccttgt ggcgacatcg
     8161 tatcagctgc gctgacatgc gcagcagtac cgattccaaa accagaaacc agtacgaaag
     8221 ctacaataat tgcttttaga tttcttctat acat
//
