LOCUS       NZ_JH815204            12656 bp    DNA     linear   CON 08-JAN-2025
DEFINITION  Barnesiella intestinihominis YIT 11860 supercont1.2, whole genome
            shotgun sequence.
ACCESSION   NZ_JH815204 REGION: 116612..129267
VERSION     NZ_JH815204.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02595395
            Assembly: GCF_000296465.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Barnesiella intestinihominis YIT 11860
  ORGANISM  Barnesiella intestinihominis YIT 11860
            Bacteria; Pseudomonadati; Bacteroidota; Bacteroidia; Bacteroidales;
            Barnesiellaceae; Barnesiella.
REFERENCE   1  (bases 1 to 12656)
  AUTHORS   Earl,A., Ward,D., Feldgarden,M., Gevers,D., Morotomi,M., Walker,B.,
            Young,S.K., Zeng,Q., Gargeya,S., Fitzgerald,M., Haas,B.,
            Abouelleil,A., Alvarado,L., Arachchi,H.M., Berlin,A.M.,
            Chapman,S.B., Goldberg,J., Griggs,A., Gujja,S., Hansen,M.,
            Howarth,C., Imamovic,A., Larimer,J., McCowen,C., Montmayeur,A.,
            Murphy,C., Neiman,D., Pearson,M., Priest,M., Roberts,A., Saif,S.,
            Shea,T., Sisk,P., Sykes,S., Wortman,J., Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform
  TITLE     The Genome Sequence of Barnesiella intestinihominis YIT 11860
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12656)
  AUTHORS   Earl,A., Ward,D., Feldgarden,M., Gevers,D., Morotomi,M., Walker,B.,
            Young,S.K., Zeng,Q., Gargeya,S., Fitzgerald,M., Haas,B.,
            Abouelleil,A., Alvarado,L., Arachchi,H.M., Berlin,A.M.,
            Chapman,S.B., Goldberg,J., Griggs,A., Gujja,S., Hansen,M.,
            Howarth,C., Imamovic,A., Larimer,J., McCowen,C., Montmayeur,A.,
            Murphy,C., Neiman,D., Pearson,M., Priest,M., Roberts,A., Saif,S.,
            Shea,T., Sisk,P., Sykes,S., Wortman,J., Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2012) Broad Institute of MIT and Harvard, 7
            Cambridge Center, Cambridge, MA 02142, USA
COMMENT     REFSEQ INFORMATION: The reference sequence is identical to
            JH815204.1.
            This is a reference genome for the Human Microbiome Project. This
            project is co-owned with the Human Microbiome Project DACC.
            Source DNA provided by Morotomi, M. (Yakult Central Institute for
            Microbiological Research, Japan).
            Bacteria provided by Morotomi, M. (Yakult Central Institute for
            Microbiological Research, Japan).
            Please be aware that the annotation is done automatically with
            little or no manual curation.
            Gene Calling Method: Prodigal.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Finishing Goal           :: High-Quality Draft
            Current Finishing Status :: High-Quality Draft
            Assembly Method          :: allpaths v. R42667
            Assembly Name            :: Barn_inte_YIT_11860_V1
            Genome Coverage          :: 130x
            Sequencing Technology    :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000296465.1-RS_2025_01_08
            Annotation Date                   :: 01/08/2025 17:37:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,797
            CDSs (total)                      :: 2,722
            Genes (coding)                    :: 2,696
            CDSs (with protein)               :: 2,696
            Genes (RNA)                       :: 75
            rRNAs                             :: 4, 4, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4 (5S, 16S)
            partial rRNAs                     :: 6 (23S)
            tRNAs                             :: 59
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 8 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 1 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12656
                     /organism="Barnesiella intestinihominis YIT 11860"
                     /mol_type="genomic DNA"
                     /submitter_seqid="supercont1.2"
                     /strain="YIT 11860"
                     /type_material="type strain of Barnesiella
                     intestinihominis"
                     /db_xref="HMP:9448"
                     /db_xref="taxon:742726"
     gene            1..3237
                     /locus_tag="HMPREF9448_RS05240"
                     /old_locus_tag="HMPREF9448_01067"
                     /db_xref="GeneID:77848366"
     CDS             1..3237
                     /locus_tag="HMPREF9448_RS05240"
                     /old_locus_tag="HMPREF9448_01067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861555.1"
                     /GO_component="GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="WP_040296069.1"
                     /db_xref="GeneID:77848366"
                     /translation="MKKNQKCKIGLLAFSLSFFVPFFANAIDQGAGKVTANVTQPSKR
                     TIKGVVSDVSGEPLIGVSVLVKGTSIGASTNIDGVYSVEVPNNNAVLEFSYVGYQKAT
                     LSLANASSYDVVMHEETRVLQEVVVTAMGISRESKTLTYATQTIKNEEVTRIKDNNFI
                     NSLQGKSAGLTIVPNNSGAGGGASKIVLRGSTSILGTNQPLIVVDGVPMQDGMGTQAT
                     DNMILGGGRSGDDLLSTINPEDIDNMTILKGPNAAALYGSAANNGVIVITTKSGASGS
                     VKVNVSSTTSIETIAMYPRTQQIYGISGGDQWSAWGPKIGTRSADEVASAPYLMNSAR
                     NAVKDFFNKGVTTTNGVTLSGGTENFRTYFSYNNTYQTGLIPNNKFTRNNVMFKESFS
                     LFDKRININTSLNWIHQKTDNAPVVGKALSALYALYRTPADIDMRYFKHNYKHLGTVA
                     DNIVSDPEKGNPKLIGQPVQTWYWYDQYLNNPYWVANMYNDYLKRDRLLANITLDAKI
                     WQNIKYQTRFNVDYVTQNNLNEEYAGMNRVGFDYVGGKYYNGDSRSSDIYNDHMLTWN
                     DRFNDKIDVNVAVGTSFSRHYDRNTSITTAIDTCGVPNAFVPQNNKHSRPNNPNGSAT
                     SASDSWNNKDWSTALFATASVGLFDKVYLDGSYRLEWAQSFQQFTQGSGYKSFDYYSA
                     GVNVLIDKFLPRRDWLNQLKWRGSWSVVGNPIPNTLFARQSYDFSNGTISTRPPLFDD
                     PQPETTTSFETGLDVWMFENKFNFDLTYYNSTLRNQFLYVTTANGESKPVNTGKIRNY
                     GLEFQASYRWNINKDWRWQTGFNIAWNDNRILETYKTESGAPYEVQMGPNAFKIKYVE
                     GGRYGDIYVNSFARDENGHIKINDAGNYENAVPVMASGKYETKVGNMTSPVTLGWNNT
                     FTWKNFTLYFLIDGRIGGKVMSLTEADLDLYGLSERSAQDRLNGERVMQNGKEYILKP
                     LPDGSGNKVSVENYYTTIGALPMEDHVYNATSLRMRDISLSYDMPNLFGKNQGMTAQF
                     SVKNAFFIYKDSPVDPDISVSAANGYSGIDCYSLPTTRSFALTLKFNF"
     gene            3270..5033
                     /locus_tag="HMPREF9448_RS05245"
                     /old_locus_tag="HMPREF9448_01068"
                     /db_xref="GeneID:77848367"
     CDS             3270..5033
                     /locus_tag="HMPREF9448_RS05245"
                     /old_locus_tag="HMPREF9448_01068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="WP_008861556.1"
                     /db_xref="GeneID:77848367"
                     /translation="MRKTIYSIGFLVIESVFSVGLFTSCGDQFQEEYPWLIGKQEEMD
                     NNNEEGAGATDITVLEKELKGAIPFMINYSHEPNGSWAPHKYQYYRANSIDNYAGYWT
                     TTKSTFAFGGPLPTLYTFPNDYLGGPMDNQIFTQSYNAIHHATELGKPEWRAVALIIQ
                     AYQGHEIVDFYGAAPFSDWRNLKRTPPLTYEKGEDIYNLIFDDLDEAIRILKERQPSR
                     EEFAKIEDLTIKTLSNGDWRMWVKFANCIKMRMAMNMVKINPGTAQSKFEQAVTDEIG
                     VLTDTDAKDIAYYQEQNACALWRIGNEWHDIRLGASFENILKRYNHPLLTRWFDTNAY
                     PIKEKSTGIQAPIDVYGVRTGISMRNSNTTGKDKGGYGPFSTLSGEFKYMHQSFFKRT
                     ESIFLLAEAALRGWNALGRDAQSWYEAGIRLCFQENGITDGTVIDEYLAQTAAKDIDY
                     VDPYNNENNIAGRVKVGVKWDASDSKEVMLEKIITQKYIANFPMSAEAWTTFRRTGYP
                     RIFPVDVNNMKDQGVDTELQLRRIPIEETANNTLEIASLVQALGQPNTGASRVFWDKQ
                     TETRGEQSPDNEFTLVIPVNF"
     gene            5053..6984
                     /locus_tag="HMPREF9448_RS05250"
                     /old_locus_tag="HMPREF9448_01069"
                     /db_xref="GeneID:77848368"
     CDS             5053..6984
                     /locus_tag="HMPREF9448_RS05250"
                     /old_locus_tag="HMPREF9448_01069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_008861557.1"
                     /db_xref="GeneID:77848368"
                     /translation="MKRYSFIFFLASFLGVTSFTACEEEATLDGATEVYITIDPMDIN
                     LKLGDTVRISATVTNVSGKVIETPVSWSIEDQTVAALLGDTAVVALDGGQEKETRLKA
                     MLENGKYALTTVTVSRNLPDGVMCIPNDSTFTEMTSKRSYNTAHDSVLFSVSPKELLY
                     DYDPVPTLEGLTAFDPVMTVDTVRGLVAVHYAAPRASGKGKVTLSIGDASTAKSASCD
                     IMLAPQLLATFYGKGFENMPYINTRPSKEVLSMYFAYTYEAEMDINSEDTVRIAMNVQ
                     SGAKEDIEAGYDAYRWEVVSSNGVLITGKTEEYVKGEGFDAVLALRSGSEEGEAEFHC
                     ITPDTVFVATFRVQDFKTRYPVEEITVDHDPISMPVGSVLRLTTGVIPASSYAYHKPV
                     VKAVDPSKVQVGEYNGNVIELRGLALGETELVLTANGKEKRVPVSVTEGILSVLWKSG
                     NARTLFEGQTVQWGIDAKTLSGGENPYDVTWTSSATDVLTAEQTGDDNTQGTITGIKA
                     GKADVIAEVAGVSSEKAEVKVIALPVDLELNASNTVKENSVVYDEGGDLVVFISPTSG
                     YGQIMLTLTDAYKGGGYAGHYDIPVGTVVDIDGARAEVTGSMDISGSGDETTVSFSIT
                     GNVGSRTLSIEADSVPVVL"
     gene            7101..10847
                     /locus_tag="HMPREF9448_RS05255"
                     /old_locus_tag="HMPREF9448_01070"
                     /db_xref="GeneID:77848369"
     CDS             7101..10847
                     /locus_tag="HMPREF9448_RS05255"
                     /old_locus_tag="HMPREF9448_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007479649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="WP_008861558.1"
                     /db_xref="GeneID:77848369"
                     /translation="MKKNLFLLSGILVLSFSSQAQELKEDYMTWPESSKLGQYVSSWV
                     LGEDLFEDENFYVSRVKPKERFRNAATQIDETLTEANDRKLVFWVPVGNASNGNTNAR
                     PNGKFDSEAFSTWSYVTHYGDWTAPHGWVPGGFADVAHKNGVGVSGVASVPWGGISSE
                     WSSGFSTLVGIEAEKVAKFLHYHGVDGLGYNSEFSTGSSFILSGLRALHETVHKYLTE
                     KGNPVVENFWYDGTNDNGQITFDSGLGNHNNDTFGDGEHIRTSLFLNYNWHGVLGGLT
                     QSTVDTYAPGRSSLDLYAGFNMQGGDPSTWRTLKDYNLSIGLWGAHDYNMLWADRANN
                     GSTDVAKQTYYQHLIEQFFTNGNRNPIDKIEVYNRGNHHPDDKWFGMSAFMTARSSLK
                     WDLSEEPFISYFNLGNGRFLNWMGERQNDNEWYNIGVQDYLPTWRWWFASDFMGKTAD
                     KVVENGLEAKFTYDDAYVGGSCLRLFGSVDNEYLHLFKTEFALSAADVITVRYKLVGG
                     QADVNLALSAKGDEQTILRESGFKVLTVADEADDEVWVEKTFKVSGSLAMLAGKEIAM
                     VALHFENAENLDLYLGEFSITRGAMPTPAAPEIKIAKVLASHYKGVDGKVIFNMDNDK
                     LPGEPVYNLDVNASMFKLYAQQKGGEPQFMGITTSWAGMYFSVPTDLQGEQEIRFGVA
                     AVSVDMKSDSEIAWSDYMSMGEYTVTDDIQINKTTIKPGESFEISYVDPRHAAADWKL
                     LNSTGEVVKEASNTTVFTVSEGIEEIGGYDLQVICDGETTTYGYYVQITSEAVGALPE
                     IQSLTIGGEAVAEDEVKIDVDEALTLGYTGRKANGASSRGIEINEGWVGGRMSDLSIG
                     DGQTFSVSAWVRFTSIPEGSCFFSVENRASSAWPVNNWGWMWSDLSSTGKLNSYTFRT
                     ATYNGAPELKYTFPNAVVTPNAWNHVVLTFEYNDQSQFRSKFYLNGVLQESTWVLDGA
                     LGTTETWANVNYSITTSDWFCFAGGRGSEPVFNNGIIDDLLVWNGAMTPEQVQNVKNG
                     LDANSLPQEVMAYWDFETDADEDHYFAAKGSVAGAKACSFKTEALGSEGAGRQVAQNP
                     LYISGCPFVPGSTFKVETRPTWTAKRGVLSESTGTDLEGSTKLTYSKPGDYTVTLTLE
                     NTLGADTKSYPVFKVGDVDGVEESMAGELRTYTVEGILFVEFAEDGDYQVGVYNASGM
                     LVAQKDAAVVAGQNMSISLGAKGVYVVKVMKEGKVVRTVKVLNR"
     gene            10986..12656
                     /locus_tag="HMPREF9448_RS05260"
                     /old_locus_tag="HMPREF9448_01071"
                     /db_xref="GeneID:77848370"
     CDS             10986..12656
                     /locus_tag="HMPREF9448_RS05260"
                     /old_locus_tag="HMPREF9448_01071"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /GO_component="GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="WP_008861559.1"
                     /db_xref="GeneID:77848370"
                     /translation="MKKSYSIFLSTLFCFLFCSLSAQDAQLNAASRKVIIEAPAEPGT
                     HLEVAYRPAGSFFPNYVQSGQQVDVGTTLIIDAYSDNPTKLPLVAVRVNGEEIEKSGS
                     GYTYVIPEGEGDIVISAEFGLLYQVDFSYSLNGRIELYAAGSEEPLTTGTCVNGNVEI
                     TVKVIPDSGCDLLSLVVNDENVTDQLANNEYKFVLKKNTSITAAFQKAYRLTVEPFEH
                     GSMRVAISDKGDLSDVPSDGKILDGQTLILWWPTVEEGYEVGSVLMNGTDITEQFFSG
                     TYNHVVKGDVNVTVTIRKKTYGLTVKDFVGGTIKAEFEDEGVVTDVSAGGRVPENATL
                     KIYEPVLDEGYELVYVSLNGSDIKDQFKDGIYSLTMKEDVMLVVKAKEIDKMFKLTID
                     EIEGGSIDVQVYVGGDLADVPADGNIKNGTMLMISEPVLEDGYELVSVMLNEEDITAL
                     FEEGVYFHTMRQDVTISVKTNYVSGVEEEQADRVRIYPNPFAESCVVSGVAAGTSVRV
                     FNMTGACVFSKVAGTGETEIRLSGLASGVYILKLEKDGDSSCHKLIRK"
ORIGIN      
        1 atgaaaaaaa atcagaagtg taaaattggg cttttggcgt tttctttgtc tttttttgtt
       61 ccgttttttg ctaatgcaat agatcaaggg gcaggaaagg ttactgcaaa tgtaacccag
      121 ccaagcaagc gtactattaa aggagtggtt tccgatgttt cgggagaacc actaatcggt
      181 gtgagtgtat tagtgaaggg aacttcgata ggagcttcta ctaatataga cggtgtttac
      241 tcggtagagg tcccgaataa taatgccgtg ctcgaattca gttatgtcgg gtatcaaaag
      301 gcgactttgt cgttggcgaa tgcttcctca tacgatgtcg ttatgcatga ggagacacgg
      361 gttttacaag aagtcgtagt aacggcgatg ggtatatcgc gggaatccaa aacgcttacc
      421 tatgctaccc agactattaa gaacgaggaa gtgacccgta tcaaggataa taacttcatt
      481 aactcgctgc aaggaaagag tgctggtctt actatcgttc ccaataattc gggagcgggc
      541 ggtggagctt ctaaaattgt gcttcgcggt tccacttcta ttctcggaac caatcagccg
      601 ttgatcgttg tcgacggtgt acctatgcaa gacggtatgg gtacgcaggc gaccgataat
      661 atgatactcg gcggcggacg tagcggcgat gacctcttgt cgacgatcaa ccccgaagat
      721 atcgataaca tgacgatttt gaaaggcccg aatgccgcag ctctttacgg tagcgcagcc
      781 aataacggtg tgatcgttat cacgaccaaa tcgggtgctt cgggttctgt caaggtgaat
      841 gtgtcgagca ccacgtcgat cgagacgata gccatgtatc ctcgtaccca acagatttat
      901 ggtatcagtg gcggagacca atggtcggcg tggggtccta agatcggtac tcgttcggcc
      961 gacgaggtgg cttctgcccc gtatctgatg aattctgcac gcaatgccgt aaaagacttt
     1021 ttcaacaaag gcgtgactac gaccaacggc gttacgttga gtggcggtac ggaaaacttc
     1081 cgaacttatt tttcttataa taatacctat caaacgggat tgataccgaa caataagttt
     1141 acccgtaata acgtgatgtt caaggaatct ttctcgttgt tcgacaagcg tatcaatatc
     1201 aacacgagcc ttaactggat acatcagaag accgataacg ctcctgttgt gggtaaggca
     1261 ttgagcgctc tctatgcttt gtaccgtact ccggccgata tcgatatgcg ttatttcaag
     1321 cataactata aacacctcgg tacggtggcc gacaatatcg tgagcgatcc ggagaaaggt
     1381 aatcccaaat tgattggaca accggttcaa acttggtact ggtatgacca atatctaaac
     1441 aacccctact gggtggccaa catgtataac gattatttga agcgcgaccg tttgttggca
     1501 aatattactt tggatgccaa gatttggcaa aatattaaat atcagacgcg tttcaatgtc
     1561 gattatgtga ctcagaataa tctgaacgag gagtatgccg gtatgaaccg ggtaggcttc
     1621 gattatgtag gtggtaaata ttacaatgga gattctcgct cgagcgacat ttataatgac
     1681 cacatgctca cttggaacga ccgtttcaat gataaaatcg atgtgaatgt ggctgtggga
     1741 accagctttt cacgtcatta cgatcgtaac acgagcatca ctacggcgat agatacttgc
     1801 ggtgtgccca atgctttcgt tcctcagaat aataaacaca gccgtcccaa caaccctaac
     1861 ggaagtgcta catcggcttc ggacagttgg aataacaagg attggagcac ggcactcttc
     1921 gctacggctt cggtcggatt attcgataaa gtttacctcg acggtagtta tcgtttggag
     1981 tgggcacagt cgttccaaca gttcacgcaa gggagcggat ataaatcgtt cgattactat
     2041 tcggccggtg tgaatgtgct catcgacaag tttttgcctc gccgggattg gctcaatcag
     2101 ttgaaatggc gcggtagttg gtcggtggta ggtaacccca ttcccaacac cttgttcgcc
     2161 cgtcagagct acgatttctc caacggtacg atcagtactc gtcctccttt gttcgacgat
     2221 cctcaaccgg agacgaccac ttcgttcgag accggtttgg acgtttggat gttcgaaaac
     2281 aaattcaact tcgaccttac ttattacaat tcgacgttac gtaaccagtt cttgtatgtc
     2341 accactgcca atggcgaatc gaagccggtc aacacaggta agatacgcaa ctatggtttg
     2401 gaattccaag catcttatcg ctggaacatc aataaggatt ggagatggca gactggattc
     2461 aatatcgcat ggaatgacaa ccgtatcctc gaaacttaca aaaccgagag cggcgctccc
     2521 tacgaagtgc aaatgggtcc caacgctttc aagattaaat acgtagaagg cggacgctat
     2581 ggcgatattt atgtcaactc tttcgcccgc gatgaaaacg gccatatcaa aatcaatgat
     2641 gccggtaatt acgagaatgc cgttcccgtg atggcttccg gtaaatacga gaccaaagtg
     2701 ggcaatatga cttcgcctgt cactctcggt tggaacaata catttacttg gaaaaacttc
     2761 acgctctatt tcttgataga cggccgtatc ggcggtaagg tgatgtcatt gaccgaggct
     2821 gatctcgacc tgtacggcct ttccgaacgt tctgcacaag accgcttgaa cggagaacgg
     2881 gttatgcaaa acggaaagga atatattctc aaacctctac ctgacggatc ggggaacaaa
     2941 gtttcggtcg agaactacta tacgaccatc ggtgctcttc ccatggaaga ccatgtatat
     3001 aatgccacca gcttgcgcat gcgtgacatc tcgttgagct acgacatgcc caatttgttc
     3061 ggtaagaatc aaggtatgac cgctcagttc tcggtgaaga atgcgttctt tatctataaa
     3121 gattctcccg tggatcccga catctcggta tcggccgcca acggttactc cggtatcgac
     3181 tgttattctt tgcctacgac gcgtagtttt gcgttaacat tgaaattcaa tttctaatga
     3241 agaacgattt aatataaata gtcatcaata tgagaaaaac aatatattcg atcggattcc
     3301 tcgttatcga atccgtgttt tcagtaggtt tgttcacttc ttgcggcgac cagtttcagg
     3361 aggagtatcc gtggttgatc ggtaagcagg aggagatgga caacaataac gaagaggggg
     3421 cgggagctac cgatattact gttctcgaga aggagctgaa aggagctatt ccgttcatga
     3481 tcaactattc gcacgaacct aacggatcgt gggcgcctca caaatatcaa tattacaggg
     3541 cgaacagtat tgacaactat gcaggatact ggacgactac caagagtact tttgctttcg
     3601 gtggtccgtt gcctacgctt tatactttcc ccaacgatta tttggggggc cctatggata
     3661 accagatttt cacacagtct tacaatgcca ttcaccacgc taccgaattg ggtaaacccg
     3721 aatggcgtgc tgtggcgttg attatccaag cctatcaagg ccacgaaatc gtcgacttct
     3781 atggcgcggc tccttttagc gactggcgta atttgaaacg tactccgccg ttgacctatg
     3841 aaaagggcga ggacatttac aatttgattt tcgacgatct cgacgaggct atacgcatat
     3901 tgaaagagcg tcagccgtcc cgcgaggagt tcgccaagat cgaggatttg actatcaaga
     3961 cgctctctaa tggagattgg cgcatgtggg tgaagtttgc caactgtatt aaaatgcgta
     4021 tggccatgaa catggtgaaa atcaatccgg gtacggctca gtccaaattt gaacaagctg
     4081 tcaccgacga aatcggtgtc ctcaccgaca ccgatgcgaa ggatatagcc tattatcagg
     4141 agcaaaacgc ttgtgctttg tggcgtatcg gtaacgagtg gcacgatatt cgtttgggcg
     4201 cttcgtttga aaatattctc aaacgttaca atcacccgct gcttacccgc tggttcgata
     4261 ccaatgcata tcctattaaa gagaagtcta cgggtataca ggctcccatc gatgtttacg
     4321 gagtgcgtac gggtatcagc atgcgtaaca gcaataccac aggcaaggac aaaggcggtt
     4381 atggcccatt ctcgacttta tcgggcgaat tcaaatatat gcatcaatct ttcttcaaac
     4441 gcaccgagag tatcttcttg ttggccgagg ctgctttgag aggctggaat gcgcttggcc
     4501 gtgacgctca gagttggtat gaggccggta tccgtctttg tttccaagag aatgggatca
     4561 ccgacggaac cgtcatcgac gaatatcttg cccagactgc ggcgaaagat atcgattatg
     4621 tcgatccgta taacaacgag aacaacatag ccggtcgtgt gaaagtgggt gtgaaatggg
     4681 atgcgagcga ctcgaaggag gtgatgctgg aaaaaatcat cactcagaaa tatatcgcca
     4741 acttccccat gagcgccgag gcatggacca cgttccgccg cacgggatat ccccgtatct
     4801 tcccggtcga tgtgaacaac atgaaagacc aaggcgtcga caccgagttg caattacgtc
     4861 gtattcccat cgaggagaca gccaacaata cgttggagat cgcttcactc gtacaggctc
     4921 tcgggcaacc caacaccggc gcttcgcgtg ttttctggga taaacagacc gaaacccgag
     4981 gcgagcaaag ccccgataac gaatttacat tggtaattcc ggtaaacttt taatctataa
     5041 aacatttgaa acatgaaacg atatagcttt atattctttt tggcgagctt cctcggtgtg
     5101 acttcgttca ccgcatgtga agaggaagct acgctcgacg gtgcgaccga ggtgtatatt
     5161 accatcgatc ccatggacat taatttgaaa ttgggcgata cggtgaggat ttcggcgacg
     5221 gttaccaatg tgagtgggaa agtgatcgag actcctgtct cttggtcgat agaagaccag
     5281 acggttgctg ccttgctggg tgataccgct gtggtggctt tggatggcgg tcaggaaaaa
     5341 gagacccggc tcaaagccat gttggagaac ggcaaatatg cccttactac cgttaccgtg
     5401 tcgcgcaatt tgcccgacgg tgtgatgtgt attcccaacg acagcacttt caccgaaatg
     5461 acttcgaaac gtagttacaa tacggcccac gattcggtgc tgttctctgt ttctcccaaa
     5521 gaacttcttt atgattatga tccggttcct actctcgaag ggctaaccgc tttcgatccg
     5581 gtgatgacgg tcgacaccgt gagaggtctc gtagccgtgc actatgcagc tccccgtgcc
     5641 tcggggaagg gaaaagtcac cctttccatt ggtgatgctt ccacggccaa gtcggcatct
     5701 tgcgatatca tgttggctcc ccagttgttg gctactttct atggcaaagg tttcgaaaat
     5761 atgccctata taaatacccg tcccagtaag gaggtattgt cgatgtactt tgcctatacg
     5821 tacgaggccg aaatggacat caattcggaa gataccgtgc gtatcgccat gaacgtgcag
     5881 tcgggcgcca aagaggatat cgaggcgggc tacgatgcct atcgttggga agtcgtttcg
     5941 agtaatgggg tattgattac gggcaagacc gaggaatatg tgaaaggcga gggattcgat
     6001 gccgtgcttg cgttgcgctc gggttcggag gaaggcgaag ccgaattcca ttgtattact
     6061 cccgacacgg tgttcgtagc gacgttccgt gtgcaggact ttaagactcg ttatccggtg
     6121 gaggaaatta cggtcgacca cgatccgatt tccatgcctg tgggaagtgt gttgagactt
     6181 acgacgggag tgattcccgc ttcgtcttat gcttatcaca agccggtagt caaggcagtc
     6241 gatccttcca aagttcaagt gggtgaatat aacggtaatg tcatagagct gaggggactc
     6301 gcattgggcg agaccgagct ggttcttacg gccaacggca aggaaaaaag ggttccggtg
     6361 agtgtcaccg agggtatctt gtctgttttg tggaaatcgg gtaatgcgcg tactcttttc
     6421 gagggacaga ctgtgcaatg gggtatcgat gccaaaactt tgtcgggagg agagaatcct
     6481 tatgacgtga cttggacttc atctgctacc gatgtgctca ctgccgagca aaccggcgac
     6541 gataacacac aggggacgat tacgggtatt aaggccggta aggcagacgt tattgccgag
     6601 gtggctggcg tatcgtccga gaaggccgag gtgaaagtga tcgctctacc tgtcgatttg
     6661 gaattgaacg cctccaacac cgtgaaagaa aactcggtgg tttatgacga gggcggcgat
     6721 ttggtagtgt tcatttcgcc cacatcgggt tatgggcaaa tcatgcttac gttgaccgat
     6781 gcctataagg gtggcggtta tgcaggacat tacgatattc ctgtcggtac ggtagtcgat
     6841 atagacggag ctcgggccga ggtgacgggc agcatggata tctcgggcag cggcgacgag
     6901 acaaccgttt cgttctcgat tacgggtaat gtgggaagtc gcactctctc tatcgaagcc
     6961 gatagtgttc ccgtggtgtt gtaaaaaaag agtcgtttcg ggataggagg ccgctaaaac
     7021 gcctcctgtc cgcggaactt gaagatagaa aaaacttttt gagaataatt caaattttta
     7081 ttaaccaaaa aaaattttcc atgaaaaaaa atctattttt attatctgga attttagttc
     7141 tttcattttc ttcgcaagca caagaattga aagaagatta catgacgtgg ccggagagtt
     7201 cgaaactcgg tcagtatgtt agtagttggg ttctcggcga agatttgttc gaggacgaga
     7261 atttttatgt ttcccgtgtg aaacccaaag aacgttttcg caatgcggcc acgcagatcg
     7321 acgagacgct caccgaggcc aacgacagaa aactcgtttt ctgggttccg gtaggaaatg
     7381 ccagcaatgg taacaccaat gcgcgtccta acggtaagtt cgattccgag gctttcagta
     7441 cttggtcata cgttactcat tacggagact ggacggctcc acacgggtgg gtccccggtg
     7501 gattcgccga tgtcgcccat aaaaatggtg taggtgtttc gggtgtggct tccgttcctt
     7561 ggggaggtat aagttcagag tggtcctctg gtttttccac gttggtgggc atagaggcag
     7621 agaaagtggc taaattcctc cattatcatg gtgtggacgg attaggctat aactcggagt
     7681 tcagtacagg ttcttctttt attttgtcgg gattgcgtgc attgcacgag acagttcaca
     7741 aataccttac ggaaaaaggt aacccggtgg tcgagaactt ctggtacgat ggaaccaacg
     7801 ataatgggca gattactttc gatagtggtc tcggtaatca taacaacgat accttcggcg
     7861 atggcgagca tatccgcacc tcgctttttc tcaattacaa ttggcatgga gtattgggag
     7921 ggttgaccca atctactgta gatacctatg cccccggtcg cagttcgctc gatctttatg
     7981 ccggcttcaa catgcagggc ggtgatcctt ctacgtggag aactctcaaa gactataatc
     8041 tctctatcgg tctgtggggc gctcacgatt ataatatgtt gtgggccgac cgtgctaata
     8101 acggtagtac agatgtagcc aagcaaacat attatcaaca tctgatcgag cagttcttta
     8161 ccaacggtaa ccgcaatccc atcgataaaa tcgaggttta caatcgcggt aaccatcacc
     8221 ccgatgacaa gtggttcggt atgtcggctt tcatgacggc acgcagttcg ctcaaatggg
     8281 atctttccga ggaaccgttt atttcttatt tcaatttggg taacggtcgt ttcttgaact
     8341 ggatgggcga acgtcagaac gataacgaat ggtacaatat cggtgtacag gactatttgc
     8401 ctacttggcg ttggtggttt gcatccgatt tcatgggtaa aacggccgac aaggtagtgg
     8461 aaaatggctt ggaggccaag tttacttacg acgatgccta tgtaggcggt tcgtgtctgc
     8521 gcctcttcgg ttctgtggac aatgagtatt tgcatttgtt caagaccgag ttcgctttgt
     8581 ccgcagccga tgtgattacg gttcgttaca aattggtagg cggtcaggcc gatgttaatt
     8641 tggcactttc tgccaaaggc gacgagcaaa cgattttgcg cgagagcggt ttcaaggtat
     8701 tgactgtcgc cgatgaggcc gacgatgaag tgtgggtgga aaagacgttt aaagtgagtg
     8761 gatctttggc tatgttggcc ggtaaggaaa tcgctatggt tgccctccat ttcgaaaatg
     8821 ccgagaacct cgacttatac ctcggtgaat tttctatcac ccgcggtgct atgcctacac
     8881 cggcggctcc cgagattaag attgccaaag ttcttgcatc tcactacaaa ggtgtagatg
     8941 gtaaggtgat atttaacatg gataacgaca aattgccggg cgagccggtt tataacctcg
     9001 acgtgaatgc ttcgatgttc aaactgtatg ctcaacaaaa aggcggtgag cctcaattca
     9061 tgggtatcac tacttcttgg gccggtatgt atttctctgt tcctaccgat ttgcagggcg
     9121 aacaggagat tcgtttcggt gtcgcagccg tttcggtcga tatgaagtcc gattccgaga
     9181 tcgcatggag cgattatatg agtatgggtg agtatacggt taccgacgat atacagataa
     9241 ataaaactac catcaagccg ggcgagagct tcgaaatttc gtatgtcgat ccccgtcacg
     9301 ccgctgccga ctggaaattg ctcaacagta ccggtgaggt cgtaaaagag gcaagtaata
     9361 cgactgtatt taccgtatcg gaaggtattg aagaaatagg cggttatgat ctgcaagtca
     9421 tttgcgacgg cgaaacgacc acttatggtt actatgtgca gattaccagc gaagccgtgg
     9481 gtgcgttgcc cgagattcaa tcgctgacta tcggcgggga agctgtggca gaagatgaag
     9541 tgaagatcga cgtggacgag gcgctgacgt tgggctatac cggaaggaaa gccaatggag
     9601 cttcttcgcg cggtatcgag atcaatgaag gctgggtagg cggaagaatg tccgatttgt
     9661 ctatcggtga cggtcaaacg ttctccgtgt cggcttgggt tcgtttcact tcgatacccg
     9721 aagggtcttg tttcttcagt gtggaaaatc gcgcatcgag tgcatggccg gtgaataact
     9781 ggggctggat gtggtctgac ttgtcatcta cgggtaaact taacagttat accttccgga
     9841 ccgctacgta caatggcgct cccgagttga aatatacgtt ccccaacgca gtcgtaacac
     9901 ccaatgcatg gaatcacgtg gtattgacat tcgagtataa cgaccagtcg caattccgct
     9961 ccaaatttta tctgaacggt gtgctccaag agtcaacgtg ggttttagat ggagctttgg
    10021 gtacgaccga aacatgggct aatgtgaatt attctatcac gacttcagat tggttctgtt
    10081 ttgccggagg ccgtggatcg gaacctgttt tcaataatgg tattatcgac gatctgttgg
    10141 tttggaacgg agccatgact cccgagcaag tgcaaaacgt gaagaacggt ctggatgcca
    10201 acagccttcc ccaagaagtg atggcttact gggatttcga aaccgatgcc gatgaagacc
    10261 actactttgc agccaaaggt tcggttgcag gggctaaagc ctgttcgttc aagacggaag
    10321 ctcttggtag tgaaggcgcc ggtcgtcaag tcgctcagaa tcctctgtat atctcaggtt
    10381 gtccgtttgt gccgggatcc actttcaagg tagagactcg tccgacttgg acagccaaac
    10441 gcggtgtcct cagcgaatcg accggtaccg acctcgaagg ttccacgaaa ctgacttata
    10501 gcaaaccggg cgattatacg gttactttga ctcttgaaaa tacgctcggg gccgatacca
    10561 agagctatcc tgtattcaaa gtaggcgatg tagacggtgt agaagagagc atggccggtg
    10621 agttgcgcac gtataccgta gagggtattc tctttgtaga atttgccgag gacggagact
    10681 accaagtggg cgtgtacaat gcctcgggaa tgcttgtcgc acagaaagat gccgctgtcg
    10741 tagccggtca aaacatgagc atctctctcg gagccaaagg cgtttatgtg gtgaaggtga
    10801 tgaaagaggg caaggtggtt cgtacggtca aagtgttgaa tcgttgatgt aatctctcac
    10861 tttcttttta agagggtgat ctcaatagcg gaccgagccc tatcatttag ggttcggtcc
    10921 ttttaacaaa tttttaatcg gtagaaaatt tatttttttg cgattatttt taatattaat
    10981 atatcatgaa aaagagttat tcgatttttt tatctacact gttttgtttt ttgttttgta
    11041 gtttgagcgc acaggatgcg cagttgaatg cggcgagtcg taaagtgatt atcgaggctc
    11101 cggcagagcc cggtactcat ttggaggtgg cttataggcc tgcgggttct tttttcccga
    11161 attatgtgca gtcggggcag caagtagacg tgggtactac gcttatcatc gatgcttatt
    11221 ctgataatcc gaccaaattg ccgttggttg ccgtacgggt gaacggggaa gagatcgaaa
    11281 aatcgggatc gggatatacg tatgttatac ccgaaggcga aggggatatt gtgatttctg
    11341 cggaattcgg tcttctttac caagtagatt tctcttattc cttgaacggg cgcatagaac
    11401 tttatgcggc gggttcggaa gaaccgttga cgacgggaac ttgtgtgaac gggaatgtgg
    11461 agataacggt gaaagttatt ccggacagcg gatgcgattt gctttctctc gtcgtgaacg
    11521 atgaaaatgt aaccgaccaa cttgcgaaca atgagtataa gttcgtattg aagaaaaaca
    11581 cttcaattac ggcggccttc cagaaagcct atcgtctgac ggtggaaccg ttcgagcatg
    11641 gtagtatgcg ggtggctatt tctgataaag gagatttgag cgatgtaccg tcggacggta
    11701 agattttgga tgggcaaacg cttatcttgt ggtggccgac agtcgaagaa gggtatgaag
    11761 taggttccgt attgatgaac gggacagata taacggagca gtttttctca ggaacgtata
    11821 accatgtggt gaaaggcgat gtgaacgtta ccgtgacgat ccgaaaaaaa acgtatggat
    11881 tgacggttaa ggatttcgtg ggaggtacga taaaagcgga attcgaagat gagggggtag
    11941 tgaccgatgt gtcggccggt ggcagagtac cggaaaatgc gaccttgaaa atttatgaac
    12001 cggtacttga tgaaggatac gaattggttt atgtgtcgct gaacggatcg gatattaagg
    12061 atcagtttaa agatggtatc tattcgctga cgatgaaaga agatgtgatg cttgttgtaa
    12121 aagccaaaga aatcgataaa atgttcaaac tgactatcga tgaaatcgag ggtggcagca
    12181 tagatgtaca agtgtatgtc ggtggagatt tggccgatgt cccggccgat gggaacatca
    12241 agaacggaac catgctgatg atttccgaac cggtactcga agacggctac gaattggttt
    12301 ccgtcatgtt gaacgaagag gatataaccg ctttgttcga ggaaggcgtg tatttccata
    12361 ccatgcgtca agatgtgacg atctccgtaa agaccaatta tgtttcgggc gtggaagaag
    12421 aacaggccga ccgggtgaga atttatccta acccgtttgc agaaagttgc gtggtgagcg
    12481 gtgttgccgc aggtacatcg gttcgcgtgt tcaacatgac gggagcttgt gtgttctcga
    12541 aagtcgccgg aacgggtgag accgaaattc gtttgtcggg acttgcttcg ggtgtctata
    12601 tcttgaagtt ggagaaagac ggcgacagct cttgccataa actgattcgg aagtaa
//
