LOCUS       NZ_BTHI01000008        14552 bp    DNA     linear   CON 11-DEC-2024
DEFINITION  Blautia wexlerae strain JCM 35486 sequence008, whole genome shotgun
            sequence.
ACCESSION   NZ_BTHI01000008 REGION: 50227..64778
VERSION     NZ_BTHI01000008.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00634933
            Assembly: GCF_045864985.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Blautia wexlerae
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1
  AUTHORS   Ye,T., Horigome,A., Odamaki,T., Kitahara,K. and Fujita,K.
  TITLE     Degradation mechanism of difructose anhydride III (DFA III) by
            Blautia species
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14552)
  AUTHORS   Kaneko,H., Horigome,A. and Odamaki,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-AUG-2023) Contact:Ayako Horigome MORINAGA MILK
            INDUSTRY CO.,LTD.; Higashihara 5-1-83, Zama, Kanagawa 252-8583,
            Japan
COMMENT     REFSEQ INFORMATION: The reference sequence is identical to
            BTHI01000008.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method       :: SPAdes v. 3.15.2
            Genome Coverage       :: 165x
            Sequencing Technology :: illumina Next seq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_045864985.1-RS_2024_12_11
            Annotation Date                   :: 12/11/2024 03:36:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,825
            CDSs (total)                      :: 3,756
            Genes (coding)                    :: 3,600
            CDSs (with protein)               :: 3,600
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 7 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 18 of 156
            Pseudo Genes (incomplete)         :: 128 of 156
            Pseudo Genes (internal stop)      :: 22 of 156
            Pseudo Genes (multiple problems)  :: 9 of 156
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14552
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="sequence008"
                     /strain="JCM 35486"
                     /isolation_source="Feces of a man in the thirties, Japan."
                     /host="Homo sapiens"
                     /culture_collection="JCM:35486"
                     /db_xref="taxon:418240"
                     /geo_loc_name="Japan"
                     /collection_date="2022"
     gene            1..2031
                     /locus_tag="ACKTSV_RS05520"
                     /old_locus_tag="JCM35486_10810"
     CDS             1..2031
                     /locus_tag="ACKTSV_RS05520"
                     /old_locus_tag="JCM35486_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="WP_022380069.1"
                     /translation="MIIYVDASVIQTGNGTKENPFKTIQEAAAKALPGDEVIVAPGLY
                     REAVNPIHAGTADKRITYRSAIKGQAHITGSEAVKDWENVEGTVWKAVIPNGIFGDYN
                     PFTTLVSGDWFIATFIAHTGDVYLNEKSMYEVTTLDKVKNPQKSTISWDPDFSVYTWY
                     AEQDEANNQTIIYANFHEKDPNKENVEISVRRNCFYPESEGIGYITLSGFRISQAATQ
                     WAPPTAYQEGMVGPHWSKGWIIEDCEIYESKCSGISLGKYLQPENDNKWLKWKYKDGT
                     QTERDCICQASYEGWDKEHIGSHIVRRCEIHDCGQTGIVGHLGGVFSVIEDNHIHHIN
                     NKQNLAGAEIGGIKMHAAIDVIFRRNHIHNCTRGLWLDWQAQGTRVTGNLFHDNALPN
                     DFEAGDDAVTSVGEDIFVEVSHGPTLIDHNILLSDRALKIATQGVALVHNLICGGFVS
                     VGIGTDNGAPDIPSPRYTPYHTKHGTQVAGFMTILHGDDRFYNNIFVQKPIRPCMQDL
                     ADLMGNNGNMWDDCNVITGTFKFNGYPTFDEWNRQFEGYCGMGSETTGNCYYDHLPVW
                     ASGNLYFNGARAWEKETDAVTDTEHTVDISVEEKEDGWYLKTNLYDIIKEENDGIIST
                     ETLGMAFEPEQKYENPDGSPIIFNQDFFGNHRDVKTVAGPFTDKKASEQKLF"
     gene            complement(2255..3622)
                     /locus_tag="ACKTSV_RS05525"
                     /old_locus_tag="JCM35486_10820"
     CDS             complement(2255..3622)
                     /locus_tag="ACKTSV_RS05525"
                     /old_locus_tag="JCM35486_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="WP_173705130.1"
                     /translation="MTTSMIKGNPLKLMLQFAFPLLLGNLLQQTYNIIDAAIVGQILG
                     AKALASVGASSSIQFLVLGFCMGSCTGFGVPVAKYFGAEKIEKMRDYIFNGAVLCAGI
                     AVILTALCSVLCPQILHILSVPEDIYDNAYSYLLIIFLGIPFTILYNYLSSILRSVGD
                     SRTPFIFLALSAVLNIFLDLFCIVVLKLGCAGAAIATISAQAISGILCLIFIIRKMKL
                     LWLKKENRTIKGDAVKELLAMGMPTGLQFSITAIGSMVMQSANNGLGSTYVSAFTAAM
                     KIKQFTMCPFDAIATSASVFCSQNLGAGQSDRIKKGLRCGITVGVGYGIAAGILLIFA
                     GHTLSMLFVGKSAVAVLDASAKYLRCMGFFYWSLGILNVARMVTQGLGYSGRAVFSGV
                     TEMIARTVVCLGFVGTFGFTAICFADQTAWITATCYIFPTCLWCIRKSTKMLASIAAR
                     VNNAS"
     gene            complement(3642..3842)
                     /locus_tag="ACKTSV_RS05530"
                     /old_locus_tag="JCM35486_10830"
     CDS             complement(3642..3842)
                     /locus_tag="ACKTSV_RS05530"
                     /old_locus_tag="JCM35486_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_055058821.1"
                     /translation="MYTGNYECLELFVKPEAGKKGSVSLKKVKENKTEIKELQHIYGN
                     WIKFRAGSLWKHDFAKRTPCEE"
     gene            complement(4177..5535)
                     /locus_tag="ACKTSV_RS05535"
                     /old_locus_tag="JCM35486_10840"
     CDS             complement(4177..5535)
                     /locus_tag="ACKTSV_RS05535"
                     /old_locus_tag="JCM35486_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005949352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="WP_407843303.1"
                     /translation="MKRNNYYDVTDWQVGNPYTDIGEVINSIIADIKNRQKDTNVNDG
                     GKPGAVIYIPSGDYHLKTQVKIDISYLKIQGSGHGFVSSSIRYNVPKEQWKDLHDIWP
                     GGSRILVDLEPLKGDERSGAAFLVEREGDPRISSVEFENFCIDGLHFVDDGNGDSENT
                     YLNGKTGIYVASAQDSFRITGMGIIYLEHGVTLYNSDALSVHDNFIAECGNCVELRGA
                     GQASKITDNLMGAGYRGYTIFAENFGGLLITSNNIFPRGKSIVHLKGVLRSSVTANRF
                     HSFYPGMLIMENCRENLISSNHFLRDHEPWPPMLEYDNGLEDDFGLIHIQGSSNSLIA
                     NHISETIEQQYLKPAGVKPIIIRLVSGRENYIANNHIVATTKTDKKESEENQSCFDAQ
                     VGALLSMDELVKLPIEAVHVDEASLDNIILDTCRENEAVMDFAENVFRGIPCLSQSAE
                     LS"
     gene            complement(5601..7082)
                     /locus_tag="ACKTSV_RS05540"
                     /old_locus_tag="JCM35486_10850"
     CDS             complement(5601..7082)
                     /locus_tag="ACKTSV_RS05540"
                     /old_locus_tag="JCM35486_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="WP_118136105.1"
                     /translation="MNKILMRQRIENAQKEIDSKKMIVQNGKMRQHYHFMPQSGWMND
                     PNGLIFFNGQYHVFYQTNPYNGFWDCMHWGHAVSKDLVHWEYLPLALAPSEEYDDYQK
                     GGCFSGSAIEHEGKLYVFYTATANHGNGSEQSQCLAISEDGINFEKYDGNPLFDAPEG
                     IQPDSFRDPKVWKHENKYYMVVGASRNNRGLALIYESADLYHWNFLNVLAESRGEWGF
                     MWECPDFYQLGDKYVLTFSPMGSGDHTSVYLTGDFDYKTGKFDWHISGEMDWGFDFYA
                     PQSMVAPDGRRLIVAWANEWEWMPLFKDWGPTYQEGWCGFFNVIREVRMCKDGTLAFV
                     PVEEMEKIRENKFTQREIQITETPQQLKAGDGISFEMKFTLDLEETDADRVELKLRCG
                     KEKESRCIFDLKNGELLVDRSCADGWSVGTSRSVLKVSEKSLDVHIFSDQSSLEIFTD
                     QYRNNHSNNIFAGNDQDAISISAFGGRAVIRDYEAYGLQECFN"
     gene            complement(7101..7931)
                     /locus_tag="ACKTSV_RS05545"
                     /old_locus_tag="JCM35486_10860"
     CDS             complement(7101..7931)
                     /locus_tag="ACKTSV_RS05545"
                     /old_locus_tag="JCM35486_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341277.1"
                     /GO_process="GO:0008643 - carbohydrate transport [Evidence
                     IEA]; GO:0055085 - transmembrane transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="WP_118136107.1"
                     /translation="MKKGNSIKLKIAVIISIIITIFWLLPYIYVICCSFKPGSEVIAV
                     PPSFLPKSFSVENFTQLFVRMNAGGYLLNSLITAVCSTIIAVILGSLAAYAIQRSGAK
                     LSVGLVVLILCLKMIPTSSIAVPIYEIICNMGLYDTRIALIIVYAAVNMPFVMWTMLS
                     FYEGVPSTLDEAAYVDGASSLQTFRKVILPICKPGLATAFIFTLFLAWNDFLISLLLT
                     SMNAKTFTVGLAGFLSAYNLDLGPMCAGAFLFSFPVMILSMFAQRYIVQGMTAGAVKG
                     "
     gene            complement(7982..8860)
                     /locus_tag="ACKTSV_RS05550"
                     /old_locus_tag="JCM35486_10870"
     CDS             complement(7982..8860)
                     /locus_tag="ACKTSV_RS05550"
                     /old_locus_tag="JCM35486_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341275.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0008643 - carbohydrate transport [Evidence
                     IEA]; GO:0055085 - transmembrane transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="WP_118136109.1"
                     /translation="MKLISKKTLLLFFLPGAIFMGAFLIYPIFKMVFDSFFKVGITGA
                     RTFIGFDNYIKAFTAGGFLKQLKNTLIYILIAVSVETVLGFIFALLFELDYKGSKIVR
                     SLMMTPLMIAPLVAGLIWKLMMSSNFGIVNEFLTRIGILSSPSDILWLADSRWSLIAC
                     CIADIWLTTPFMMLMILAGLQGLDTSVAEAARMDGASKLQEIFYIKIPGIKPVLLTAL
                     SVRIIDAAKTFDIIWAMTEGGPNSSSETISIIIYKTLVKYNNTGYASAMAVIFIIVLV
                     VFTLVFMQSLWNPKKK"
     gene            complement(9040..10269)
                     /locus_tag="ACKTSV_RS05555"
                     /old_locus_tag="JCM35486_10880"
     CDS             complement(9040..10269)
                     /locus_tag="ACKTSV_RS05555"
                     /old_locus_tag="JCM35486_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341273.1"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="WP_118136111.1"
                     /translation="MRKKIVNSLITATVIGSMLTGMAVPCFAGGKKDGGSSITVLVES
                     GSPAEALANNTAADFEKETGCKVVVDAVAYTGMYDKLSTEIKAGQAAHDVSCMDFVWL
                     AAFADAIEPITDADTSDFLPTLEESGTVDGNLLGYPMWVNAKILIYRKDLIPEDKVPK
                     TWDEYKALAKEMKTDDMYGTTVFGSGSDAVCSFLDFACQAGADGLVYDKDGNVNITDQ
                     PYVDALDFMVEMANEDCTPSDSLATASTESQELFNNGKVAMQLNWSHQYPAAVEALGA
                     DKVGCAPMIAGSAGVGATTGPWYECVMKNSENKDMALKYVEYMYDHNADYMNGTLKIA
                     GRTSVYEEAGKEAGNEHTTAVLDTLNEKQSQPRPMISTWSQVEQVLTGVVESCLGGAD
                     VKETLESAKEEIEAIGK"
     gene            complement(10615..11595)
                     /locus_tag="ACKTSV_RS05560"
                     /old_locus_tag="JCM35486_10890"
     CDS             complement(10615..11595)
                     /locus_tag="ACKTSV_RS05560"
                     /old_locus_tag="JCM35486_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="WP_118136113.1"
                     /translation="MAGIKDVAKMAGVGVGTVSRMLNDSGYVSLDTRAKIEAAMKELN
                     YTPNELARNLYYKKSGIIAVLVPNVSNPFFAEFVDCVEGELRKAGFKIMLCNTLKDVK
                     AEAEYLDLLNRHIVDGIIAGMSSLDESEYSKIHKPIVALDRYLGEEIPVVAVDHKMGG
                     RLAAEVLLRNGCKRILHFRSTAEKESLYHDRHAEFQKIMDEQGIETYCYDLDWRRLDI
                     QYYHQVAEEVMEKNLKFDGVFGVDRLAIECMNGLIRQHKKIPEEVKIVSYDGTYITEL
                     VEPQISTIVQPIDRMAEESVKNLCELINGKKVKNKKSILIPEFRKGGTTV"
     gene            complement(11987..12097)
                     /locus_tag="ACKTSV_RS05565"
     CDS             complement(11987..12097)
                     /locus_tag="ACKTSV_RS05565"
                     /inference="COORDINATES: protein motif:HMM:NF042606.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6783 domain-containing protein"
                     /protein_id="WP_334295134.1"
                     /translation="MFSVARYDALIRTKSPTNCDAHFAESIFQTHSRNSD"
     gene            <12071..12256
                     /locus_tag="ACKTSV_RS05570"
                     /pseudo
     CDS             <12071..12256
                     /locus_tag="ACKTSV_RS05570"
                     /inference="COORDINATES: protein motif:HMM:NF042606.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6783 domain-containing protein"
     gene            complement(12368..12826)
                     /locus_tag="ACKTSV_RS05575"
                     /old_locus_tag="JCM35486_10900"
     CDS             complement(12368..12826)
                     /locus_tag="ACKTSV_RS05575"
                     /old_locus_tag="JCM35486_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019163044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="WP_019163044.1"
                     /translation="MILKEKERSVIEDLQTQEKSCVEKYGKYAEQARDPELKNLFRNI
                     QQEEQKHYESLSMILSGSVPECNCNDRSGKDYQPKAVYTAMSSPEDKQHDAFLATDCI
                     GTEKLVSGTYNDDVFAFGDSGVRKLLADIQIEEQNHAEMLYKYKTVNGMA"
     gene            13185..14552
                     /locus_tag="ACKTSV_RS05580"
                     /old_locus_tag="JCM35486_10910"
     CDS             13185..14552
                     /locus_tag="ACKTSV_RS05580"
                     /old_locus_tag="JCM35486_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705109.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="WP_008705109.1"
                     /translation="MKRIDFENGTVTNNILSAALPMLVAQILNLLYNIVDRIYIARIH
                     DIGTTALGAVGLCFPIIMIITAFSNLFGSGGAPIFSINRGKGDSRTADMIMNTAFTML
                     CGSAAVLMLIGFLFARPLLTLFGASDDALVYAYPYLMIYLLGTLPSMIATGMNPFINA
                     QGYAIIGMLSVTVGAVANIILDPIFIFVLDMGIKGAAIATVISQILSALLVFYFLHGK
                     SELKVRWIHINEISECTRHARDIISLGSAGFIMQLTNSLVSICCNNVLSVTGGDIYIS
                     VMTIISSVRQMVETPIHAINEGTSPVLSYNYGARRPDRVKKAGVVLIIMVLIYTGIMW
                     SVILIAPEFLIGIFSSDKLLLKDAVPALKLYFAAFIFMDLQYIGQTVFKSLNKKKQAI
                     FFSLLRKVFIVVPLTYFLPYGLHMGTDGVFMAEPVSNVIGGSLCFITMLVTILPELNK
                     MGNSR"
ORIGIN      
        1 atgattattt acgttgatgc atccgtgatc cagaccggaa acggaacaaa agaaaatcca
       61 ttcaaaacta ttcaggaggc cgctgcaaag gcacttccgg gagatgaagt catcgttgca
      121 ccgggattat accgtgaagc tgttaatccg attcacgctg gaacggcaga caagcgtatc
      181 acctaccgtt ctgcaataaa aggacaggcg cacattacag gaagtgaagc tgtcaaagac
      241 tgggaaaatg tagaaggcac tgtatggaaa gcagtcattc cgaacggaat cttcggtgat
      301 tataacccat ttactaccct ggtatccgga gattggttta tcgctacatt tattgctcat
      361 acaggagatg tatatctgaa tgaaaaatcc atgtatgaag taaccactct ggataaagtt
      421 aaaaatccac aaaaaagtac tatctcatgg gatcctgatt tctctgtata tacatggtat
      481 gcagaacagg atgaagccaa taatcagaca attatttatg caaacttcca tgaaaaagat
      541 cccaataaag aaaatgttga gatcagtgta agaagaaact gcttctatcc agaaagtgaa
      601 ggaatcggat atatcactct ctccggtttc cgcatcagtc aggctgctac tcagtgggct
      661 ccccctaccg cttatcagga gggaatggtc ggtcctcact ggtccaaagg atggatcatc
      721 gaagactgtg aaatctatga atccaaatgc agcggtattt ctcttggtaa atatctgcag
      781 cctgaaaatg ataacaaatg gttaaagtgg aaatacaaag acggaactca gacagaacgc
      841 gactgcatct gccaggcttc ctatgaagga tgggataaag aacacatcgg ttcccatatt
      901 gtgcgcagat gtgagatcca tgactgcggt cagacaggta ttgtaggaca tcttggaggc
      961 gtattctctg ttattgaaga caaccatatc caccacatta ataacaagca gaatctggca
     1021 ggagcagaga ttggcggtat caaaatgcat gctgctatcg atgttatttt ccgccgcaac
     1081 catatccaca actgcacccg tggtctgtgg cttgactggc aggcacaggg aacccgcgtt
     1141 acaggaaatc tcttccatga caatgctctt cctaatgact tcgaggcagg ggatgacgct
     1201 gtcacaagcg taggagaaga tatcttcgtt gaggtttccc atggtccgac attgatcgac
     1261 cacaatatcc tgctgtctga ccgtgcactg aaaattgcaa cccagggtgt tgcccttgta
     1321 cataacctga tctgcggcgg cttcgtttct gttggtatcg gtacagacaa cggtgcacct
     1381 gatattccat ctccgagata tactccatat cacaccaaac acggaactca ggttgcagga
     1441 tttatgacca tccttcatgg agatgacaga ttctacaaca acatcttcgt tcagaaaccg
     1501 atccgtcctt gtatgcagga tctggctgat cttatgggta acaatggaaa tatgtgggat
     1561 gactgcaatg tgatcacagg aactttcaaa tttaacggat atccaacctt tgatgaatgg
     1621 aacagacagt tcgaaggtta ctgcggaatg ggttctgaaa caacaggcaa ctgctattat
     1681 gatcatcttc ctgtatgggc atccggcaat ctctatttca atggagcacg tgcatgggaa
     1741 aaagaaacag atgcagtcac agatacagaa cacactgtag atatctctgt tgaagagaaa
     1801 gaagatggct ggtatctgaa gaccaacctg tatgatatca tcaaagaaga aaacgacggt
     1861 atcatctcaa ctgaaacact tggtatggca ttcgaaccgg agcagaaata cgaaaatcct
     1921 gacggaagcc cgattatctt taatcaggat ttctttggaa atcacagaga tgtcaaaact
     1981 gtcgcaggac catttacgga taaaaaagca tctgagcaga agctctttta acctgatatc
     2041 catatagtca atagctttac catatataaa ccatacataa aaacagctat gaagagtaca
     2101 gatattttta tctgcagctc ttgcatagct gttttcttat tttgtcatac ttttttatag
     2161 cagtcctaat aaataatgca tttaagacag ttcagcagag tgcgaaagac aaggaagata
     2221 tctgacgcag aatttcacac tctgatgggc tggattatga tgcattattt actcgggctg
     2281 ctatagatgc aagcattttt gtagactttc ggatgcacca caggcatgtc ggaaaaatat
     2341 agcaggttgc tgtgatccag gcagtctggt ctgcgaagca gatggctgtg aagccaaaag
     2401 ttcctacaaa tccaagacag acaactgttc tggcgatcat ttctgttacg ccggagaaaa
     2461 cggctctgcc tgaatatcca agtccttgag ttaccatacg ggcgacattg agaataccaa
     2521 gactccagta gaaaaatccc atacatctga ggtatttggc agaggcatcc aaaactgcca
     2581 ccgcactctt tccaacaaac agcatagaaa gtgtatgccc tgcaaaaatc agtaaaattc
     2641 ctgccgcaat accatatcct actcccactg taataccaca gcgcaatcct ttcttaatcc
     2701 tgtcagactg tcctgctcca agattttggc tgcagaatac agaagcagag gttgcgatag
     2761 catcaaatgg acacatggta aactgcttga tcttcattgc tgcagtaaaa gctgatacat
     2821 atgtacttcc aagcccgttg ttcgcagact gcataaccat acttccgata gctgtgatgg
     2881 agaactgaag tcccgtaggc attcccattg ccagaagttc ctttactgca tcccctttta
     2941 ttgtcctgtt ttctttcttg agccacagaa gtttcatttt gcggataata aaaataaggc
     3001 acaagattcc actgattgcc tgtgcggaaa tggttgcaat ggcagctccg gcacatccaa
     3061 gtttcagcac tacgatacag aataaatcca gaaaaatgtt cagaacagca gataacgcca
     3121 gaaatataaa cggtgtcctg ctgtctccca ctgacctcaa aatactggac agatagttat
     3181 ataaaatcgt aaagggaatc cccaggaaaa tgattagcag ataactatat gcattgtcat
     3241 aaatgtcctc tggtacagat aaaatatgca gaatctgagg acataaaaca ctgcataatg
     3301 ctgtcaggat cactgcgatt cctgcacata gtacagctcc attaaagata taatcccgca
     3361 ttttttctat tttctctgca ccgaaatatt tagctacagg aacaccaaag cctgtacagc
     3421 ttcccatgca gaatcccagt accagaaact gaatactgct gctggctcct acacttgcca
     3481 gtgcttttgc gccaagaatc tggcctacga tcgcagcatc aataatatta taggtctgtt
     3541 gcagcaggtt tcctaacagt aaaggaaacg cgaactgtaa catcagtttc agcggattgc
     3601 cttttatcat agatgtagtc atttctattt atccctcatt ttcattcttc acatggagtt
     3661 ctcttggcaa agtcatgctt ccataagctc ccggctcgga acttgatcca gttcccatag
     3721 atatgctgca gttcttttat ttctgttttg ttttctttta ccttcttcag gctcacactg
     3781 ccttttttcc cggcttctgg ttttacaaat aattccagac attcatagtt tcctgtgtac
     3841 atatgtaaaa atcccctttc tgaattttgt ctccgattta ctatatatct atagtactga
     3901 ccttctgccg acataatctt actatatcat agcaaaatca cgaggagttt caataaaata
     3961 ttttgacatt tttttatact attttatcct gtatgatccc atataaaaag gatgccgaga
     4021 gcaaatccga catccttttt actgttaact tatgtttatt tacagtagcc tcgttataac
     4081 tatacgagag tattataaat tttcccagtt ctctttgatt actgtgcttt ttttctttta
     4141 aaatcatgaa attttttaca atttataata atgcaatcaa gacagttccg cagactgcga
     4201 aagacaagga attccccgaa acacattttc tgcaaaatcc ataactgctt cattttctct
     4261 gcatgtatcc agtattatat tatccaatga tgcctcatca acatgtactg cttcaatcgg
     4321 aagtttaacc agttcatcca tacttaacaa tgctccaacc tgcgcatcaa agcaggattg
     4381 attttcttca ctttcttttt tatcagtttt tgtagttgcc acaatatgat tgtttgcaat
     4441 gtaattttct cttccggaaa ccaaacgaat gattatcggt tttacgcctg ccggtttcag
     4501 atattgctgt tcaatcgtct ccgaaatgtg attagcaatc agtgaattgc tacttccctg
     4561 aatatgaatc aggccaaaat catcctctaa tccattatca tattccagca tcggaggcca
     4621 tggttcatga tctctgagaa aatgattgga agatatgaga ttttctctac aattttccat
     4681 tatcagcatt cccggataaa aagaatgaaa tctgtttgca gttactgatg atctaagaac
     4741 acccttaaga tgtactatac tttttcctct tggaaaaatg ttattggaag taatcagaag
     4801 tcctccaaaa ttttcggcaa aaattgtata acctctataa ccagccccca taagattatc
     4861 tgttatttta gatgcctgtc cggctccacg aagttctaca caatttccac attctgctat
     4921 aaaattatca tgaacagata atgcatcaga attgtaaaga gtcactccat gctccagata
     4981 aataataccc atgcctgtaa ttctgaaaga atcctgtgca ctggctacat aaattcctgt
     5041 ttttccattt aaatatgtat tctctgaatc tccatttccg tcatctacaa aatggagtcc
     5101 atcaatacag aaattttcaa actccacgga actgattctc ggatctccct ctcgttccac
     5161 cagaaaagca gcaccggatc tttcatcccc ctttaatggt tccaaatcta ccagaatcct
     5221 gctaccgccc ggccagatgt catgaagatc cttccactgt tcttttggca cattatatcg
     5281 gatacttgaa gacacaaatc catgtcctga tccttggatt ttcaagtaac taatgtcaat
     5341 tttcacctgc gtttttaaat gataatctcc agacggaata taaattactg ctcccggttt
     5401 tcctccatca tttacatttg tatccttctg tctgttcttg atgtctgcaa tgatactgtt
     5461 gatcacttct ccgatatccg tatatgggtt acctacctgc cagtcagtga catcataata
     5521 attattacgt ttcatttcaa tactcctcct gctttcagtt catgtcaagc ggatattcgc
     5581 aatccgctcc gctacttgat tcagttaaaa cactcctgca atccatatgc ttcatagtct
     5641 ctgattaccg ctcttccacc aaacgcagat atacttattg catcctgatc atttcctgca
     5701 aaaatattat tggaatgatt atttctgtat tgatctgtaa atatctccag agaactctga
     5761 tctgaaaaga tatgtacatc cagacttttt tcgctgacct taagtacact tctggatgtt
     5821 cctacactcc atccatctgc acagcttctg tctaccagca attcaccatt tttcagatca
     5881 aaaatacaac gggattcttt ttcttttccg catctgagct tcagttctac cctgtctgca
     5941 tcagtttctt caagatccaa tgtaaatttc atctcaaagc tgattccatc tcctgctttt
     6001 aactgctgag gtgtttctgt aatctgtatt tcccgttgcg taaatttgtt ttcacggatt
     6061 ttttccattt cctctacagg aacaaacgcc agcgttccat ccttgcacat ccgaacttca
     6121 cgaatcacat taaaaaatcc gcaccagccc tcctgataag taggtcccca gtccttaaag
     6181 agaggcatcc attcccattc attcgcccag gcaacaatca gacgtcttcc atcaggagca
     6241 accatggact gtggcgcata aaaatcaaat ccccaatcca tttcaccact gatatgccag
     6301 tcaaattttc ctgttttgta atcaaaatct cctgtcaggt atactgatgt atgatctcct
     6361 gagcccattg gagaaaaagt gagaacatat ttatcgccaa gctgataaaa atccggacat
     6421 tcccacatga atccccattc tccacggctt tctgccagca cgtttaaaaa attccaatga
     6481 taaagatctg cagattcata tatcaatgct aatcctctgt tattacgact ggcccctaca
     6541 accatataat atttattttc atgcttccat acctttggat cgcggaatga atccggctga
     6601 ataccttccg gtgcatcaaa gagaggattt ccgtcatact tttcaaaatt tattccatct
     6661 tcacttatag caagacactg gctttgctca cttccgtttc catgatttgc agttgccgta
     6721 taaaatacat ataacttacc ttcgtgttcg attgcactgc cggaaaaaca tccacctttt
     6781 tgataatcat catactcttc actgggtgcc aatgccagag gcaaatattc ccaatgaact
     6841 aaatctttgc tgactgcatg tccccaatgc atacaatccc aaaatccatt atatggatta
     6901 gtctgataaa acacatgata ctgtccgtta aaaaagatca gtccgttagg atcattcatc
     6961 cacccgctct ggggcataaa atgataatgc tgacgcattt ttccattttg aacaatcatc
     7021 tttttactgt caatttcttt ttgcgcgttt tctattcttt gtctcattaa tattttattc
     7081 actggctttc tccattctta ttatcctttt acagctcctg ctgtcattcc ctgaacaatg
     7141 tatctctgtg caaacattga taaaatcatt accgggaagc taaataaaaa tgctcctgca
     7201 cacatcggtc caaggtcaag attatacgct gacaggaaac cggcaagacc tactgtaaat
     7261 gttttagcat tcatactggt cagcaacaga gaaataagaa aatcattcca tgccaggaac
     7321 aatgtaaata taaaggcagt tgcaagtccc ggcttacaaa tcggcaaaat aacctttctg
     7381 aatgtctgaa ggctggaagc gccatctaca tatgcagcct catccaaagt agacggtact
     7441 ccctcataaa aactaagcat tgtccacatt acaaaaggca tgtttactgc cgcataaaca
     7501 ataatcaatg caattctggt atcatacaat cccatattac aaataatctc ataaataggt
     7561 actgcaatac tggatgtagg aatcattttc agacataaaa tcaataccac caaccctact
     7621 gacagcttcg ctccagatct ctgaatagca tatgcagcca gtgaacccaa gataactgca
     7681 ataattgtac tgcaaactgc tgtaatcaaa ctgttcagca gatacccacc cgcgttcatt
     7741 cttacaaata attgagtgaa attttcaacc gaaaagcttt ttggaagaaa agatggcgga
     7801 acagcaatta cctctgatcc aggtttaaac gaacaacaaa ttacataaat atatggaagc
     7861 agccagaata tcgtaataat tatagaaata ataactgcta tttttagttt gatactatta
     7921 cctttcttca ttttggtctc cttattgaat ccttcttatt actgtttttc tttgacagct
     7981 tttacttttt ctttggattc cataaactct gcataaatac taatgtaaac actacaagaa
     8041 ctatgataaa gataacagcc attgcactgg catatcctgt attattatac tttacaagtg
     8101 ttttataaat aataatacta attgtctccg atgaactgtt aggtccacct tctgtcattg
     8161 cccagataat gtcaaaagtc tttgctgcat ctataattct cactgacaat gctgtcaaca
     8221 gaaccggttt aattcccggt atttttatgt aaaaaatttc ctgcagctta cttgctccat
     8281 ccattcttgc agcttctgcc acgctagtat ccaatccctg aaggccagcc agaatcatca
     8341 gcatcataaa cggtgtagtc agccatatat ctgcaataca gcaggcgatc aatgaccatc
     8401 tggaatctgc aagccacaaa atatcacttg gactggacaa gattcctatt ctggtcagaa
     8461 actcattgac aataccaaag tttgaactca tcatcaattt ccagataaga cctgcaacca
     8521 gcggtgcgat cataagtggt gtcatcatta atgaacgtac tattttactg cccttataat
     8581 caagttcaaa taataatgca aaaataaatc ctaaaactgt ttccacagat accgcaatca
     8641 aaatataaat caatgtattt ttcagctgct tcaaaaaacc gccagctgta aatgctttaa
     8701 tataattatc aaatccaata aaagttctgg ctcctgtaat tccaacctta aagaaactgt
     8761 caaataccat tttaaaaata ggataaatca aaaaggcacc catgaaaatc gcgccaggaa
     8821 gaaaaaacag caatagtgtt ttttttgata tcaatttcat aatatttccc gctttcttat
     8881 aaaaatcaag ggcgatttac tccctgcatc atacatcata gattagtaaa ttcatagtta
     8941 gtgtgttcga tgtccaggta aaatcaccct tgatttaatt acatttttta ttatttggta
     9001 gatgtctgca atctgctttt tctttattac tttgtcactt tacttcccaa ttgcctcaat
     9061 ctcttctttt gcagattcaa gggtttcttt cacatctgct ccaccaagac aggattctac
     9121 aacacctgta agaacctgct ctacctgtga ccaggttgaa atcataggtc ttggctgaga
     9181 ctgtttttca ttcaatgtgt caagaactgc tgttgtatgc tcattaccag cctcttttcc
     9241 agcttcctca taaacagaag ttctgccggc aattttaagg gttccgttca tataatctgc
     9301 attatgatcg tacatatatt ctacatattt cagtgccatg tctttattct cagaattttt
     9361 cattacacat tcataccatg gacctgtggt tgctcctact cctgcactgc cggcaatcat
     9421 tggtgcacat cccaccttat ctgctccaag tgcctcaaca gctgcaggat actgatggct
     9481 ccaattcaac tgcattgcaa ctttaccatt attgaaaagt tcctgagact ctgtagaagc
     9541 agtagccaga gaatctgatg gcgtacaatc ttcatttgcc atttcaacca taaaatcaag
     9601 cgcatccaca tatggctgat ctgtaatatt tacatttcca tccttgtcat atactaatcc
     9661 atctgcaccg gcctggcagg caaaatcaag gaaggaacaa actgcgtcag aaccacttcc
     9721 aaaaactgtt gttccgtaca tatcgtctgt tttcatttct tttgccagtg ctttatattc
     9781 atcccaggtc tttggcactt tatcttccgg aatcagatct tttctgtaaa taaggatctt
     9841 agcgtttacc cacatcggat atcccagaag attaccatct actgttccgc tttcttccag
     9901 cgttggaagg aaatcactgg tatctgcatc tgtaattggt tcgattgcat cagcaaaagc
     9961 tgccagccat acaaaatcca tgcatgaaac atcatgagca gcctgtcctg ctttaatttc
    10021 ggtagaaagc ttatcataca taccagtata ggcaacagca tcaacaacaa ccttacagcc
    10081 tgtttctttc tcaaaatcag ctgctgtatt attggcaagt gcttctgcag gtgaaccact
    10141 ttctacaaga actgtaatac tgcttccacc gtcttttttc ccacctgcaa aacagggaac
    10201 tgccattcct gtaagcatac ttccaatcac agttgctgta attaatgaat taacaatctt
    10261 ttttctcatt tttaatctcc tattcttaaa gattctaaat tccatgatat taaatatcat
    10321 gccgttccat ttacatactg cttcaaccgc tttagatatt ctccggagta atctaatttt
    10381 acgggttaac attgggaaaa caaattttgt tttacaatgt ggaacgcgtt ccacattgtt
    10441 taaaaaattt gttttcttcg tcttgttgat ttcattataa cctttttgta gcttttgtca
    10501 atccaattat ggttttttta ttttttttat aaattatgtc caaatatttt gtacttattt
    10561 ttatttttca tcctttattt tgtaacaaat gcacaacgca cgtatgacat ctgtttatac
    10621 tgttgttccg cctttacgga attcaggtat taaaatactt tttttatttt tcactttttt
    10681 tccattaatt agctcacaaa ggttttttac agattcctct gccattctgt caatgggctg
    10741 aacaattgta cttatttgcg gttctaccaa ttctgtaata tacgttccat cataagaaac
    10801 tatttttacc tcttccggga tttttttgtg ttgcctgatc aatccattca tgcattcaat
    10861 agccagtcgg tctacaccaa acacaccatc aaatttcaga tttttttcca taacttcttc
    10921 tgcaacctga tgataatact gaatatccaa acgccgccag tccagatcat aacaataggt
    10981 ttctattccc tgctcatcca taattttttg aaattctgca tgacgatcat gatataaaga
    11041 ctctttttct gcagtacttc tgaaatgaag tattcttttg cagccattac gcagcaacac
    11101 ttctgctgcc aggcgtccac ccattttatg gtccacagct actaccggaa tttcttctcc
    11161 cagatatctg tcaagcgcaa caatcggctt atgaatttta gaatactcac tttcatccaa
    11221 agaagacatt cccgcaatta tcccatctac aatatgtctg ttcagcaaat ccagatattc
    11281 agcttcagcc tttacatctt tcaatgtgtt acataacata attttgaagc cggctttgcg
    11341 cagttcccct tctacacaat caacaaactc agcaaaaaaa ggattggata cattcggtac
    11401 aagtaccgca attataccac tttttttata atacagattt ctggcaagtt cattcggtgt
    11461 ataattcaat tccttcatag cagcttctat tttggccctc gtatccagag aaacatatcc
    11521 actgtcatta agcattcttg aaactgttcc cacacctact cctgccattt ttgcaacatc
    11581 tttaatccct gccatttttt ccacactttc atattatgat attaaattcc caagttccaa
    11641 atttgttcaa gcatacacga tgagatattt aaacttagaa cttgtcaaac attaagaaac
    11701 aacgatttaa gtaataaatc agcaaatttt ctaaaatttg ggatttctta actccattta
    11761 gaagatacct gaaattcatc atctaaagat ataccttctg caaataaatt atttttaata
    11821 ttatcagacc aaaaaaatcc aatacaaata ctccaacaaa tataatacat tactaataaa
    11881 taatatacta ctaatgagta gtgtattata tttaccatat tcattcaagt atttataaaa
    11941 aattcttatt cattcctgcc aaaattcact attacacctt acgtaactaa tcggaattcc
    12001 tagaatgtgt ttgaaaaatg ctttccgcaa aatgtgcatc acaatttgta ggagattttg
    12061 ttcgaataag ggcgtcgtag cgagctacgg aaaacaaatt ttggcaaaat atatcgcaaa
    12121 gtggggcgtg cagattatgg gaatgatttt tcaaacacgc tctagcaatt atcttatggc
    12181 ttttatcatg acaaatttta caaaattttt cataaaatat cccaacattt ataccatcat
    12241 attttttgta aaataagatg tacctggcaa gagacagtca aaaccgattg tctcctgtca
    12301 ggtaattata gcagtcctgg tatagaattt tcccaatatg ctactctgct caatgcctta
    12361 agacttctta tgccattcca ttcacagtct tatacttata cagcatctct gcatgattct
    12421 gctcctcaat ctgtatatca gccaacagtt tgcgtacacc actatcgcca aaggcaaata
    12481 catcatcatt gtatgttcct gacactagct tctctgtacc gatacaatcc gttgcaagaa
    12541 aggcatcgtg ttgtttatct tccggtgaac tcatggctgt atagactgct tttggctgat
    12601 agtctttgcc tgatctgtca ttacagttac attcaggcac acttccggaa agaatcattg
    12661 acaaagactc ataatgcttc tgttcttcct gctggatatt ccggaaaaga tttttaagct
    12721 ccgggtctct ggcctgttct gcgtattttc catacttttc cacacagctt ttctcttgtg
    12781 tctgaagatc ttcgattact gaacgttcct tttcttttaa aatcatagtc tgttgtctcc
    12841 ttttttaaga aatactgtat tttgttacat gaaaatagta tggataaaaa atgctttttt
    12901 attctttata tcatgactta tgacaatttc agagaatatt ttgtgttact gttcacatgc
    12961 cactgtcccg cgaggtgttt tggcatgaat atgccaaaat cccgagacat acaagccaaa
    13021 attccattgc cgcaggcaat ctggaatgaa ttttggctac gttactgtga acggagtgaa
    13081 caataactat tttgttccaa caccactgta tcagggtgaa tttctgttta ctttatgata
    13141 tacttatatg cacgataaaa gttttaaact taggaagatt tataatgaaa cgaattgatt
    13201 ttgaaaacgg aactgtgacc aacaatatat taagcgctgc gcttcccatg ctggtagcgc
    13261 agatattgaa tcttttatac aacattgtgg accgtatcta tatcgctagg atccatgata
    13321 taggaacaac tgcacttggc gctgtgggct tatgctttcc gatcatcatg atcattactg
    13381 ctttcagtaa tctctttggt tccggaggtg caccaatttt ctctatcaac cgcggaaaag
    13441 gagattccag gactgctgac atgatcatga atactgcttt caccatgtta tgtggaagtg
    13501 ctgctgtgct gatgctgatc ggatttcttt ttgcccgccc cttactgact ctgtttggtg
    13561 cttcagatga tgctctggtt tatgcgtatc cctatctgat gatttacctt ctgggtactc
    13621 tcccttccat gatagcaaca ggaatgaatc cttttatcaa tgcacaggga tatgccatta
    13681 tcggtatgct gtcagtaaca gtcggtgcag tggcaaatat tattcttgat ccgattttta
    13741 tttttgttct ggatatgggg attaagggtg cagcgattgc tactgtcatc tctcagattc
    13801 tctctgcctt gcttgtattt tattttcttc atggaaaatc agaattgaag gtaagatgga
    13861 ttcatataaa tgagatttct gagtgcacca gacatgccag ggatatcatc agtctggggt
    13921 ctgccggatt tatcatgcag ctgactaaca gcctggtaag tatctgctgc aataatgttc
    13981 tttccgtgac aggcggtgat atttatatct ctgtcatgac tattatatcc agtgttcgcc
    14041 agatggtgga gacgcctatc catgcgatca atgagggaac ctctcctgtt ctcagctaca
    14101 attacggagc cagacgtcct gacagggtaa agaaagcagg ggttgttctg atcattatgg
    14161 ttctgatcta tacaggtatc atgtggagcg tcattctcat tgcccctgaa tttctgatcg
    14221 gaatcttcag ttcagataaa ttgttattga aggatgccgt tcctgcgctg aagctttact
    14281 ttgcagcctt tatttttatg gatctgcaat atattggtca gacagtgttt aaatcattaa
    14341 ataaaaaaaa gcaggcgatt tttttctccc tgcttcgtaa agtatttatc gtggttccgc
    14401 tgacttattt cctgccctat ggattacaca tgggcacaga tggtgtgttt atggcagaac
    14461 ctgtatccaa tgtgatcggt ggttctctgt gttttattac catgctggtt acaatattgc
    14521 ctgaattgaa caaaatggga aactcccgat aa
//
