LOCUS       NZ_GG657685            10695 bp    DNA     linear   CON 27-FEB-2025
DEFINITION  Blautia hydrogenotrophica DSM 10507 Scfld7, whole genome shotgun
            sequence.
ACCESSION   NZ_GG657685 REGION: 174321..185015
VERSION     NZ_GG657685.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02415620
            Assembly: GCF_000157975.1
KEYWORDS    WGS; RefSeq.
SOURCE      Blautia hydrogenotrophica DSM 10507 (Ruminococcus
            hydrogenotrophicus DSM 10507)
  ORGANISM  Blautia hydrogenotrophica DSM 10507
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 10695)
  AUTHORS   Sudarsanam,P., Ley,R., Guruge,J., Turnbaugh,P.J., Mahowald,M.,
            Liep,D. and Gordon,J.
  TITLE     Draft genome sequence of Blautia hydrogenotrophica DSM 10507
            (Ruminococcus hydrogenotrophicus DSM 10507)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10695)
  AUTHORS   Fulton,L., Clifton,S., Fulton,B., Xu,J., Minx,P., Pepin,K.H.,
            Johnson,M., Bhonagiri,V., Nash,W.E., Mardis,E.R. and Wilson,R.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JAN-2009) Genome Sequencing Center, Washington
            University School of Medicine, 4444 Forest Park, St. Louis, MO
            63108, USA
REFERENCE   3  (bases 1 to 10695)
  AUTHORS   Fulton,L., Clifton,S., Fulton,B., Xu,J., Minx,P., Pepin,K.H.,
            Johnson,M., Bhonagiri,V., Nash,W.E., Mardis,E.R. and Wilson,R.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JAN-2009) Genome Sequencing Center, Washington
            University School of Medicine, 4444 Forest Park, St. Louis, MO
            63108, USA
COMMENT     REFSEQ INFORMATION: The reference sequence is identical to
            GG657685.1.
            Blautia hydrogenotrophicus (GenBank Accession Number for 16S rDNA
            gene: X95624*) is a member of the Firmicutes division of the domain
            bacteria and has been isolated from human feces. The sequenced
            strain was obtained from Deutsche Sammlung von Mikroorganismen und
            Zellkulturen GmbH (DSMZ) (DSM 10507) and was previously known as
            Ruminococcus hydrogenotrophicus.
            
            * The available GenBank Accession Number is for the 16S rDNA gene
            of a related strain, not the sequenced strain.
            
            This is a Newbler assembly
            (http://www.454.com/enabling-technology/the-software.asp) comprised
            of 1/2 plate FLX PE 454 Data with a Q20 coverage of 21.8X.
            
            This sequenced strain is part of a comprehensive, sequence-based
            survey of members of the normal human gut microbiota.  A joint
            effort of the WU-GSC and the Center for Genome Sciences at
            Washington University School of Medicine, the purpose of this
            survey is to provide the general scientific community with a broad
            view of the gene content of 100 representatives of the major
            divisions represented in the intestine's microbial community. This
            information should provide a frame of reference for analyzing
            metagenomic studies of the human gut microbiome. Further details of
            this effort are described in a white paper entitled 'Extending Our
            View of Self: the Human Gut Microbiome Initiative (HGMI)'
            (http://www.genome.gov/Pages/Research/Sequencing/SeqProposals/HGMIS
            eq.pdf). These studies are supported by National Human Genome
            Research Institute.
            
            For answers to your questions regarding this assembly or project,
            or any other GSC genome project, please visit our Genome Groups web
            page (http://genome.wustl.edu/genome_group_index.cgi) and email the
            designated contact person.
            Blautia hydrogenotrophica (GenBank Accession Number for 16S rDNA
            gene: X95624*) is a member of the Firmicutes division of the domain
            bacteria and has been isolated from human feces. The sequenced
            strain was obtained from Deutsche Sammlung von Mikroorganismen und
            Zellkulturen GmbH (DSMZ) (DSM# 10507).
            
            * The available GenBank Accession Number is for the 16S rDNA gene
            of a related strain of Blautia hydrogenotrophica, not the sequenced
            strain.
            
            This is a Newbler assembly
            (http://www.454.com/enabling-technology/the-software.asp) comprised
            of 1/2 plate FLX PE 454 Data with a Q20 coverage of 21.8X.
            
            This sequenced strain is part of a comprehensive, sequence-based
            survey of members of the normal human gut microbiota.  A joint
            effort of the WU-GSC and the Center for Genome Sciences at
            Washington University School of Medicine, the purpose of this
            survey is to provide the general scientific community with a broad
            view of the gene content of 100 representatives of the major
            divisions represented in the intestine's microbial community. This
            information should provide a frame of reference for analyzing
            metagenomic studies of the human gut microbiome. Further details of
            this effort are described in a white paper entitled 'Extending Our
            View of Self: the Human Gut Microbiome Initiative (HGMI)'
            (http://www.genome.gov/Pages/Research/Sequencing/SeqProposals/HGMIS
            eq.pdf). These studies are supported by National Human Genome
            Research Institute.
            
            Coding sequences were predicted using GeneMark v3.3 and Glimmer2
            v2.13. Intergenic regions not spanned by GeneMark and Glimmer2 were
            blasted against NCBI's non-redundant (NR) database and predictions
            generated based on protein alignments. tRNA genes were determined
            using tRNAscan-SE 1.23 and non-coding RNA genes by RNAmmer-1.2 and
            Rfam v8.0. Gene names are generated at the contig level and may not
            necessarily reflect any known order or orientation between contigs.
            
            For answers to your questions regarding this assembly or project,
            or any other GSC genome project, please visit our Genome Groups web
            page (http://genome.wustl.edu/genome_group_index.cgi) and email the
            designated contact person.
            
            This is a reference genome for the Human Microbiome Project. This
            project is co-owned with the Human Microbiome Project DACC.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000157975.1-RS_2025_02_27
            Annotation Date                   :: 02/27/2025 01:54:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,532
            CDSs (total)                      :: 3,466
            Genes (coding)                    :: 3,386
            CDSs (with protein)               :: 3,386
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 24 of 80
            Pseudo Genes (incomplete)         :: 65 of 80
            Pseudo Genes (internal stop)      :: 5 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10695
                     /organism="Blautia hydrogenotrophica DSM 10507"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Scfld7"
                     /strain="DSM 10507"
                     /isolation_source="biological product [ENVO:02000043]"
                     /host="Homo sapiens"
                     /type_material="type strain of Ruminococcus
                     hydrogenotrophicus"
                     /db_xref="taxon:476272"
                     /synonym="Ruminococcus hydrogenotrophicus DSM 10507"
     gene            1..984
                     /locus_tag="RUMHYD_RS09740"
                     /old_locus_tag="RUMHYD_02194"
     CDS             1..984
                     /locus_tag="RUMHYD_RS09740"
                     /old_locus_tag="RUMHYD_02194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005949338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="WP_021845150.1"
                     /translation="MAGIKDVAKRARVGVGTVSRMLNDSGYVAEGTREKIEIAMKELN
                     YTPNELARNLYHKRTGIIAVLVPNVSNPFFSEFVDHVEAELYETGFKMMLCNAAKACS
                     AEREYLDMLNRHIVDGVITGVHSLDVEEYKKIHKPIVALDRYLGEHIPVVAVDHKEGG
                     RLAAETLIDCGCRKILHFKGATAVKSPYHDRHYEFERVMKKNQVETYSYELEWNRFDS
                     EYYREVVADVFSKEIDFDGVFAVDELAIECMNEAIRRHFKVPRDVKFVAYDGTFITEM
                     VEPKMTAVVQPIASLAKESVRLLNELIGGREYKNKRVVLGVQIRKGNTTIS"
     gene            <1835..2832
                     /locus_tag="RUMHYD_RS09745"
                     /old_locus_tag="RUMHYD_02196"
     CDS             <1835..2832
                     /locus_tag="RUMHYD_RS09745"
                     /old_locus_tag="RUMHYD_02196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341273.1"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="WP_005949343.1"
                     /translation="DKLSTEIKAKQAAHDVACMDVVWLAAFADAIEPVTGADTSDFLP
                     TLEDSGTIDGNLLGYPMWVNSKILIYRKDLIPEEKVPKTWEEYQNLAKELTTEEMTGT
                     TVFGSGSDGVCSFLDFACQAGAEGLIFDEDGNVNITDQAYVDALNFMVENANADYTPD
                     DSLSTAATESQELFTNGKIAMQLNWSHQYPAAVEALGEDKVGCAPMIAGDAGIGATTG
                     PWYECVMKSSENKEMAQKYVEFMYDHNADYMDLTLKIAGRTSVYEEAAKEPGNEHTTA
                     VLETLGAEQSQPRPMVSTWPQIEEVLTGVVESCLGGADVEETLETAKTEIETIGK"
     gene            2921..3805
                     /locus_tag="RUMHYD_RS09750"
                     /old_locus_tag="RUMHYD_02197"
     CDS             2921..3805
                     /locus_tag="RUMHYD_RS09750"
                     /old_locus_tag="RUMHYD_02197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341275.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0008643 - carbohydrate transport [Evidence
                     IEA]; GO:0055085 - transmembrane transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="WP_005949345.1"
                     /translation="MRLISRKTLLLFFLPGAIFMCAFLIYPIITMVIDSFFEIGLTGD
                     KTFIGLENYIHAFTAGGFLKQLKNTLIYIVVAVGVETVIGLLFALFFELNYKGSKVVR
                     SLMMAPLMIAPLVAGLTWKLMMSSSFGIVNELLTRIGILSSSSDILWLADERWSLLAC
                     CIADIWLTTPFMMLMILAGLQGLDSSMVEAAKIDGANMLQQIFYVKLPVIKPVLLTAL
                     SVRIIDAARTFDIIWAMTEGGPNSSSETISITIYKTLTRYNDIGYASSMAVIFIVVLV
                     IFTLVFMQSLWNPKKKNG"
     gene            3830..4660
                     /locus_tag="RUMHYD_RS09755"
                     /old_locus_tag="RUMHYD_02198"
     CDS             3830..4660
                     /locus_tag="RUMHYD_RS09755"
                     /old_locus_tag="RUMHYD_02198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341277.1"
                     /GO_process="GO:0008643 - carbohydrate transport [Evidence
                     IEA]; GO:0055085 - transmembrane transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="WP_005949347.1"
                     /translation="MKKKGEVWKRLGIGLSVILTVFWLFPYLYVVCCSFKPGSEVIAV
                     PPSFFPKVFSVENFSGLLERMDAMKYLLNSLSVSLISTVIALILGALAAYAIQRSGAK
                     LSILLVVLVLCLKMIPTSSIVVPIYELICNLGLYDTRIALVIVYSAINMPFVMWTMLS
                     FYEGIPTSLDEAAYVDGASSLQTFRKVILPICKPGLATAFIFTLFLAWNDFLVALLLT
                     STNAKTFTVGLAGFLSAYNLDLGPMCAGAFLFSFPVMIISLAAQKYIVQGMTAGAVKG
                     "
     gene            4679..6154
                     /locus_tag="RUMHYD_RS09760"
                     /old_locus_tag="RUMHYD_02199"
     CDS             4679..6154
                     /locus_tag="RUMHYD_RS09760"
                     /old_locus_tag="RUMHYD_02199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005949348.1"
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="WP_005949348.1"
                     /translation="MSKELMKQNIEKAQREIDSKKDIVKKGKMRQRYHFMAQTGWLND
                     PNGLIYFRGKYHFFFQHNPYNGFWDSMHWGHAVSDDMLHWEYLPLALAPSETYDNHLR
                     GGCFSGSAIEHDGKLYLMFTGATNEGKGNEQTQCIAYSEDGIHFEKYAGNPVLIAPEG
                     VPTDCFRDPKVWKHEDTYYMVCGASRDNKGQALLYRSKDMIHWTYFNVLAESRGEWGY
                     MWECPDFYPMGDKYVLTFSPMGAGEHTSVYLVGDFDYLTGKFCCHVSGEIDWGLDYYA
                     PQSFLAPDGRRIIVGWSNEWEWMPLWKDWGPTYKEGWCGFFNIPREVRMRKDGTLQFL
                     PIREVETIRENPKRIAELAVSEEYTELEAGDGVCFELKLKIDLQRTNADELELDLRCG
                     AERRTICLFHFKKGEMRVDRNAADGWSKGVSRSVLYLQGKKELDVHILSDQSSLEIFT
                     DQYQNNHSNNIFAKASQNQLRVRARGGNAVLRDIETYGLRA"
     gene            6185..7153
                     /locus_tag="RUMHYD_RS09765"
                     /old_locus_tag="RUMHYD_02200"
     CDS             6185..7153
                     /locus_tag="RUMHYD_RS09765"
                     /old_locus_tag="RUMHYD_02200"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005949350.1"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="WP_005949350.1"
                     /translation="MRKEYDVTALGELLIDFTENGSSGQGNPLLEANPGGAPCNVLAM
                     LQKLGRKTAFIGKVGQDLFGSTLRETIEAVGINSEGLVMDKDVHTTLAFVHTFPDGDR
                     EFSFYRSPGADMMLRREEVLKEKIQASRIFHFGTLSFTHESVKEASEYAIQCAKEAGA
                     WISFDPNLREPLWENLEEAKKAMEYGMECCDILKIADNELTFITGETDYDKGALYLKE
                     KYQIPLVCVTLGKNGSRAYYKDWITAAEPFLQENTIETTGAGDTFTGCILDTVLEKGL
                     EELTKEDLRQMLRFANAAAALITTKKGALKVMPEKVEIEQLLSGKG"
     gene            7184..8539
                     /locus_tag="RUMHYD_RS09770"
                     /old_locus_tag="RUMHYD_02201"
     CDS             7184..8539
                     /locus_tag="RUMHYD_RS09770"
                     /old_locus_tag="RUMHYD_02201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005949352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="WP_005949352.1"
                     /translation="MAGKNYYDVTDWNTGNPYEDIGEVINSIIADIKCRQSEADVEEG
                     GKPGAVIYIPSGDYHLRTQVVIDISYLKIMGSGHGFVSSSIRYNLPESEWADLHEVWP
                     GGSRILVEISAKDTGEEAAGAAFYVERNGNPRISSVEFENFCIDGLHFTDDGSGVENP
                     ENTYTNGKTGIYVASAQDSFRLTGMGIVYLEHGVTIYHADALSVHDNFIAECGNCLEL
                     RGWGQASKITDNLMGAGYKGYSIYAQNFGGLLIGSNNIFPRGKSSIHFDNVARSSITG
                     NRFHSFYPGMLVFEGNCSENLVSSNHFLRDNEPWAPMLEYNNGLNDLYGLLCISGNYN
                     SIIANHISESIHGQSIHPAGEKPVIIHLTSGMGNYISSNHIVATTEASSEQTEASDSC
                     FSAQVDALTMTDQRVPLEVIAVLVEKESIQNTVLDTGMDSQVIMDKSANAFRATPGIG
                     E"
     gene            complement(8676..9410)
                     /locus_tag="RUMHYD_RS09775"
                     /old_locus_tag="RUMHYD_02202"
     CDS             complement(8676..9410)
                     /locus_tag="RUMHYD_RS09775"
                     /old_locus_tag="RUMHYD_02202"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005949353.1"
                     /GO_function="GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]; GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="WP_040350648.1"
                     /translation="MENLKLWEDLKNWKKNCDFVELSHEFSPDTPHWSGFPAMNAEMI
                     FDYPDVFRVHIVQVATQYGTHVDAPAHFVPGKRMLNELRADEMVLPLCVIDLSAKSAK
                     NCDYIFTAEDLHQWEAIHGEIPSGSFVAVRTDWSKRADLDNMDEKGMKHYPGWGLDAL
                     KFLVEQRNVTAIGHETSDTDAPVSSAKIGYVGEYYILEQERYQIELLKNLDKVPATGA
                     VIICGFPRGVNLTGFTARCIAVCPKD"
     gene            complement(9460..>10695)
                     /locus_tag="RUMHYD_RS09780"
                     /old_locus_tag="RUMHYD_02203"
     CDS             complement(9460..>10695)
                     /locus_tag="RUMHYD_RS09780"
                     /old_locus_tag="RUMHYD_02203"
                     /inference="COORDINATES: protein motif:HMM:NF012382.5"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="WP_005949355.1"
                     /translation="FIGGDNFKEGGNAMPKFAQRIYDMESSAKIIRALFGAMDSPNII
                     SLGGGAPASDALPVEAVHDICTDVITRNKRGVEALQYGSVLGVSDLRKNVADILLKPK
                     GLEAELDEIIIVNGGLETMNLLCQVYINPGDVILVESPTFVHCVEIFEMFQARCVGVE
                     CDDCGMILEDVEKKIQKLHPKLVYIIPTFQNPTGKTFPLERRKKLAEIGSKYDVIILE
                     DDPYQELRYSGEPLPPIKCFDKTGHTVYANSFSKIFSPGSRLGFCHASHDIITKLFDA
                     KTATNSHTSTFAQVVCAEFFNRGLFSSQLKKLCEIHRERRDVMMNCFAKYMPGGTKWV
                     HPDGGLFSWVELPGGIDTAALLPEANAAGIHYIAGSSFYVQGGNMGSNAMRVSFGNVT
                     PEKIDIAVQQLASLVKNKL"
ORIGIN      
        1 gtggcaggga ttaaggatgt ggccaagaga gcgcgtgtcg gtgtggggac agtatcccgg
       61 atgctgaacg atagcggcta tgtggctgag ggaaccaggg aaaagattga gattgctatg
      121 aaagaactga attatactcc caatgaactt gccagaaatc tatatcataa gcggacaggg
      181 attattgcgg ttcttgtccc gaatgtgtcg aatccttttt tctcagagtt tgtcgatcac
      241 gtggaggctg agctatacga aaccggattt aagatgatgc tctgcaatgc ggcaaaggca
      301 tgcagcgcag agcgcgagta tctggatatg ctgaatcgtc acattgtgga tggggtgatt
      361 acaggagtcc actctctgga tgtggaagag tacaaaaaga ttcataagcc tattgtagcc
      421 ttggatagat atctgggaga gcatattcca gtggtagcag tcgaccataa ggaaggtggt
      481 cgtttggcag cagagacact gattgattgt ggatgcagaa aaattctgca ttttaaaggt
      541 gcgacagcgg tgaaatcccc ctatcatgac aggcactatg agtttgagcg tgttatgaag
      601 aagaatcagg tagaaaccta ttcatatgaa ctggaatgga atcgttttga ttctgagtat
      661 tatcgagaag tcgtcgcaga tgtgttttcg aaagaaattg atttcgacgg tgtatttgcg
      721 gtagacgaat tggccattga atgtatgaat gaggcgatac ggagacattt caaagtgccc
      781 cgggacgtga agtttgtcgc ttatgacggg acttttatca cggagatggt ggagcctaag
      841 atgacggctg ttgtacagcc gattgccagc cttgcaaaag agtctgtgcg gttgttaaat
      901 gagctgatag gcggaagaga atataaaaat aagcgggttg ttctaggcgt acagatcaga
      961 aagggaaata ctacaatttc ttaatgagaa ttgcacaaaa tgatatgtat attatctggt
     1021 atattgcaca aagtgtagag aaataagtaa aaatacattg acaaacagga cataagtaat
     1081 ataatgaaat tgtaaagagg atttctcttt atttgttttt aaacttatgt ggaacgcgtt
     1141 ccacatattt ttagagcaga tatggaacgc gttccacatt tgttgtgtaa ataagaaaag
     1201 ccttgtcacg ctaatgcgcg aaagtctttt cctaattaca caaacgtttc gcgtggatcg
     1261 cactgcggcg gagtgaaaga tggcgctttg cgccccgccg caggcggaga atcctgcaag
     1321 caggattctt ttttataaaa gcagaatatg atgaaccatt ccaaaatatt tagatagaaa
     1381 ttttggacgg tattttggaa aagggacatt atataaattc cgtatgcagc tccagcgtga
     1441 tttcagaagg gcttcgggca gtgaatgaac gtggacagac aagcactgct tgcagtaaaa
     1501 tgaatgtctg agagagagat ggggaataaa ttatggaaac ggaatcacaa caatttataa
     1561 gacgaaaggg aggacaagaa tgaaaaagaa gattctaagt gttttgctgg cggcagcaat
     1621 ggtgggaaca atggcgtctg gatgcggttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatgata agctttctac agagatcaag
     1861 gcaaaacagg ctgcgcacga tgtggcatgt atggatgtgg tatggctggc agcatttgcc
     1921 gatgccatcg aaccggtgac tggggcagac acgagcgatt ttcttccgac tttggaagac
     1981 agcggtacga tcgacggaaa tcttcttgga tatccgatgt gggtaaactc gaagatactg
     2041 atatacagaa aagatctgat tcctgaggag aaggttccaa agacctggga agagtatcaa
     2101 aacctggcca aagaactgac gacagaggag atgactggta caacggtatt tggtagtggt
     2161 tctgatggtg tgtgttcttt ccttgacttc gcctgccaag ctggtgcaga ggggttgatt
     2221 tttgacgagg atggcaacgt gaacattaca gatcaggctt atgtggatgc tctgaacttt
     2281 atggtggaga atgcgaatgc ggattatact ccagacgatt ctctgtctac agcagctaca
     2341 gagtctcagg agctgtttac caatggaaag attgcaatgc agctgaactg gagtcatcag
     2401 tatccggcgg cagtggaagc tctcggagaa gataaagtcg gctgtgcgcc aatgatcgca
     2461 ggagatgccg gtatcggagc gacgactggc ccatggtatg agtgtgtgat gaagagctct
     2521 gaaaataaag agatggcaca gaagtatgtg gagttcatgt atgaccacaa tgctgactac
     2581 atggatttga cgctgaagat tgcgggcaga acttccgtat atgaggaggc agcaaaagaa
     2641 ccgggcaatg agcacactac agcagtttta gagactctgg gcgctgagca gtctcagcca
     2701 agacctatgg taagcacctg gcctcagatt gaagaagttc tgactggagt cgtagagtcc
     2761 tgtctgggcg gagcagatgt agaggaaact ttggagacgg caaagactga aattgagacg
     2821 ataggcaaat aatcataaga aatgaattga cagagggcgt tccagaaagc ggcagctgac
     2881 aaaaaggaag gccctctatt tgaagaggtg gtaagagaag atgagattga tttcgagaaa
     2941 aaccttactg ttattctttc tgccgggcgc catattcatg tgtgcgtttc ttatatatcc
     3001 gattatcaca atggtaattg acagtttctt tgaaattggg ctcacgggag ataaaacgtt
     3061 tattggactg gaaaactata tccatgcgtt tacagccggc ggttttttga agcagttgaa
     3121 aaatacattg atttacattg ttgtggctgt gggagtggag acggtgatcg gcctgctgtt
     3181 cgcgctgttt tttgaactga attataaagg gagtaaagtt gtccgttccc ttatgatggc
     3241 gcctcttatg attgcaccac tggttgcagg tcttacctgg aaactgatga tgagttccag
     3301 ctttggaatt gtcaatgaac ttctgacaag aatcggaatt ttgtccagca gtagcgatat
     3361 tctctggctg gctgatgaga ggtggtcgct gctggcctgc tgtattgcag atatctggct
     3421 tacaacaccc tttatgatgc tgatgatact ggctggcctg caagggctgg attccagtat
     3481 ggtagaggcg gccaaaattg acggggccaa tatgttacag cagattttct atgtgaagct
     3541 tccggtgatc aaaccggttc tgctcacagc gttgtctgtg agaatcatag atgctgcaag
     3601 gacttttgat attatctggg cgatgacgga gggcggtccg aatagttcct ctgagacaat
     3661 cagcattacg atctataaga cactgacgcg ttataacgat atcggttatg cgagctcaat
     3721 ggcagtaatc tttatcgtag ttttagtgat ctttacactg gtatttatgc agagcttatg
     3781 gaatccgaag aaaaagaacg gctagttggt ttgagaggag gcaaaaagag tgaagaaaaa
     3841 aggtgaagtc tggaaaagac tgggaatcgg tttgtcagtg attcttacgg tattttggct
     3901 ttttccgtat ttatatgtgg tttgttgttc ctttaaaccg ggctcagaag taattgcggt
     3961 tccgccaagc tttttcccaa aggttttctc tgttgagaat ttttcaggat tgttagagag
     4021 aatggatgcg atgaagtatt tgctgaacag tttgagtgtt tctctgataa gcacagtcat
     4081 tgcgctgatt cttggagcgc tcgcagcata tgcgattcag cgttcaggtg caaagctttc
     4141 tattctgttg gtcgttctgg tgctgtgtct gaagatgatt cctacatcaa gtattgtcgt
     4201 gccaatttat gaattgattt gcaatctggg tctatatgat acgagaattg ccttggttat
     4261 tgtgtattca gctattaata tgccctttgt gatgtggaca atgctgagtt tttatgaggg
     4321 aattcctaca tcattggacg aggcggcgta tgtggacggt gcgagcagtc tgcaaacatt
     4381 ccgaaaggtg attttgccga tttgtaagcc tggtttggcg actgcattta tttttacatt
     4441 attccttgcc tggaatgatt tccttgtggc gttgttattg actagcacga acgcaaagac
     4501 gtttaccgtt ggactggcag ggttcttgtc ggcctataat ttggatttgg gaccaatgtg
     4561 tgcaggagca tttttattta gtttcccagt gatgattatt tcactggcag cccaaaaata
     4621 tatcgtacag ggaatgacgg caggggcagt aaaaggttaa aagtaatgga ggagtcttat
     4681 gtctaaagag ttgatgaaac agaatattga aaaggctcag cgagagatag atagtaagaa
     4741 agatattgta aagaaaggaa agatgcgtca gcgttatcac tttatggcgc agaccggatg
     4801 gttaaatgat cccaatggtt tgatttattt tagaggaaaa taccatttct tttttcagca
     4861 taatccgtat aatggatttt gggacagtat gcattgggga cacgcggtca gcgatgatat
     4921 gctacactgg gaataccttc ctttggcatt ggcgcccagt gagacctatg ataatcactt
     4981 gagaggaggc tgcttttctg gaagcgctat tgaacatgac ggcaaactgt atttaatgtt
     5041 cacaggggcc acgaatgagg gaaaaggaaa tgagcagaca cagtgtattg catacagtga
     5101 agatggaatt cattttgaaa aatatgcggg aaatcctgtg ctgattgctc cagagggtgt
     5161 tccgacggac tgttttcgag acccgaaggt gtggaaacat gaagacactt attatatggt
     5221 gtgcggggcg agccgagaca ataagggaca ggcgctgctg taccggtcga aggatatgat
     5281 tcactggacc tattttaatg tactggctga gagccgcgga gaatggggat atatgtggga
     5341 atgtccggac ttttatccta tgggggataa atatgtgctg acgttctcgc ccatgggtgc
     5401 cggagaacat acctcggtat atctggtggg tgattttgat tacctgacag gaaaattctg
     5461 ctgccatgta agcggtgaaa tcgattgggg gttagactat tatgcacctc aatctttctt
     5521 ggccccagat gggagaagga ttatcgtcgg atggtccaat gaatgggagt ggatgccttt
     5581 gtggaaagat tgggggccga cctataagga aggttggtgc ggatttttca atatcccaag
     5641 ggaagtgcgc atgaggaaag atggcacgct gcagtttttg ccgatcagag aggtggagac
     5701 gatacgggag aatccaaaaa gaatagcaga actggcggtc tcggaggaat acacagagct
     5761 ggaagccgga gatggtgtgt gctttgagct gaagctgaag atagatcttc aaagaacaaa
     5821 tgcggatgaa ctggaattgg atctgcgctg cggagcagaa agaaggacga tctgtctgtt
     5881 tcatttcaaa aaaggggaaa tgcgtgtgga cagaaacgcg gcggacggct ggagtaaggg
     5941 agtttccagg agcgttctgt atttgcaagg gaaaaaagaa ttggatgtgc atattttatc
     6001 tgatcagagt tcccttgaaa tttttacgga ccagtatcag aataatcatt ccaacaatat
     6061 attcgcaaaa gcttcgcaga atcagctgag agttcgtgcc cgtggaggca atgcagtcct
     6121 tagagatatt gagacttatg gattaagagc gtaagaacaa acggcgcgaa ggagggcaaa
     6181 gagaatgcgt aaggaatacg atgtgacggc tttgggagag cttttgattg attttacaga
     6241 gaatggaagt agcgggcagg ggaatcctct gttggaagcg aatccaggcg gagcgccgtg
     6301 caatgtgctg gcgatgctac agaagctggg aagaaagacg gcgtttatcg gaaaggtagg
     6361 acaggatttg tttggcagta cgttacggga gacgatagaa gctgtgggaa tcaattctga
     6421 ggggctggtg atggacaaag acgtacatac aacgcttgca ttcgtgcata cgtttcctga
     6481 cggggaccgg gagttttctt tttaccgtag tccgggagcc gacatgatgc tgagaagaga
     6541 ggaagtctta aaggaaaaga ttcaggcttc caggattttt cactttggga ctttatcttt
     6601 tacccatgag agtgtaaagg aagcctcaga atatgcgatt cagtgtgcaa aagaggctgg
     6661 agcttggatt tcttttgacc ccaatctgcg ggaaccgctt tgggagaatc tggaggaagc
     6721 gaaaaaagcc atggaatacg gaatggaatg ctgtgatatt ttaaagattg cggacaatga
     6781 actgacgttt ataactgggg agacggacta tgacaaaggg gcactgtacc ttaaagaaaa
     6841 gtatcagatt cccctggtct gtgtgacact ggggaaaaat ggcagtaggg cttactataa
     6901 ggactggatt accgcggcgg agccgttttt gcaggaaaat accatagaga ccaccggcgc
     6961 gggggatact ttcaccggat gtattctgga tacagtgctt gaaaagggct tagaggaact
     7021 tacaaaggaa gatctcaggc agatgcttcg tttcgccaat gccgctgcgg cattgattac
     7081 gacgaaaaaa ggagctttaa aggtgatgcc tgaaaaagtg gagatagaac agctgttgtc
     7141 agggaaaggc tgagtatgga caatcacgta ggagggcaaa aggatggctg gtaaaaatta
     7201 ttatgatgta acagattgga acactggaaa tccttacgag gacatcggag aagtgatcaa
     7261 cagtatcatc gcagatataa agtgcagaca atcggaggct gatgttgagg aaggcggaaa
     7321 accaggtgcg gtgatctata tcccgtcggg ggattaccat cttcgcacgc aggtagtgat
     7381 tgacatcagc tatctgaaaa ttatgggttc aggacatggg tttgtgtctt ccagtattcg
     7441 ttataatctg ccggagagcg aatgggcgga tttacacgaa gtatggccgg gaggcagccg
     7501 tattctggtg gaaatttctg cgaaagacac aggggaggaa gcggcaggag cagcgtttta
     7561 tgtggagaga aacggaaatc ctcgtatcag ctcggtggaa tttgagaatt tctgcattga
     7621 cggactgcat tttacagatg atggctcagg agtggagaac ccagaaaata cctatacgaa
     7681 tggaaaaacg ggaatctatg tagccagtgc gcaggactcc tttcggctta ccggaatggg
     7741 aattgtgtat ctagaacatg gcgtcacgat ttatcatgcg gatgcgttgt ctgtgcatga
     7801 taattttatc gctgaatgtg gaaactgcct ggaattaaga ggctggggcc aggcgtccaa
     7861 gattaccgat aacctcatgg gagcggggta taaaggatat tcaatttatg cccagaattt
     7921 tgggggactt ttgatcggct ccaataatat ttttcccaga ggaaagagca gtattcattt
     7981 tgacaacgtg gcacgttcga gtatcaccgg gaatcgtttt cattcttttt atccgggaat
     8041 gctggtgttt gagggaaatt gttcagaaaa tctggtctct tccaatcatt ttctgcgtga
     8101 caatgaaccg tgggcgccga tgctagaata taacaatggt ttgaacgatc tatatggtct
     8161 tctgtgtatc agcggcaatt ataattccat tattgccaat catatttcag aatccattca
     8221 tggacaaagc attcatccgg ccggtgaaaa accggtgatt atccatctta cgtcgggcat
     8281 gggaaattat atttcttcca accatatagt tgccacgaca gaagcttcca gtgagcagac
     8341 agaagcatcc gattcttgtt tttccgcgca ggtagatgcg ctgacgatga cggatcaaag
     8401 ggtgccgttg gaggtgatag cggtactggt ggaaaaggaa tccatacaaa ataccgtgtt
     8461 ggatacagga atggacagcc aggtaatcat ggataagtcg gcaaatgctt tcagagcaac
     8521 tccaggaatc ggagagtaag aagagggttg tgcagatcct tctacggtag ggagagggtg
     8581 aatcggaaag ttcaggaaac accgcggtat agattcatgg ctttgcaaaa gcttgaatct
     8641 ataccgcggt gtttcctttt ttctgtcagg aaggtttaat ctttaggaca gacggcaatg
     8701 cagcgggcgg taaaaccggt aagattcact ccgcgtggaa aaccacagat gatgacagcg
     8761 ccggttgcgg gaactttgtc gagatttttc aggagttcaa tttgatagcg ctcctgttcg
     8821 agaatatagt attctcccac ataaccgatt tttgctgagc tgactggagc gtcagtatca
     8881 ctggtctcat ggccaatggc ggtgacattg cgctgttcta cgaggaattt aagcgcgtca
     8941 agcccccagc cgggataatg tttcatgcct ttttcatcca tgttgtccag gtcggcgcgc
     9001 ttgctccagt ctgtgcgcac ggcgacgaag ctgccggatg ggatttcgcc gtggatcgcc
     9061 tcccactggt gcagatcttc ggctgtgaag atatagtcac agtttttggc tgattttgcg
     9121 ctgagatcta tgacgcagag tggcagcacc atctcgtcgg cgcgtagttc gttaagcatc
     9181 cgtttgccag gcacgaagtg tgctggggcg tctacgtggg tgccatactg tgtggcgact
     9241 tgtactatat gaacgcggaa tacatctgga tagtcaaaaa tcatttctgc gttcatggct
     9301 gggaaaccgg accaatgcgg cgtgtctggt gagaactcat gagagagttc cacaaaatca
     9361 cagttttttt tccagttctt gagatcctcc caaagtttaa gattctccat ttgttttcct
     9421 cctttttctg agggcgcatt gtatttcagt tttgcgtcct tacagtttgt ttttgacaag
     9481 tgaggcgagc tgctgtacgg cgatgtcgat tttttcaggt gtaacattac cgaagcttac
     9541 gcgcatagca tttgaaccca tgttaccgcc ttgaacgtag aagctggaac cagctatgta
     9601 gtggataccg gcagcattgg cttcaggcag cagtgcagca gtgtcaatgc ctcctggcag
     9661 ctccacccaa ctgaagagcc ctccgtcagg gtgtacccac ttcgtgccgc ctggcatata
     9721 tttagcgaag cagttcatca tcacatcacg gcgttcacgg tggatctcac agagcttttt
     9781 gagctgagag gaaaacagtc cgcggttgaa gaattcggcg cagacgacct gagcgaaggt
     9841 agaggtgtgc gaattcgttg cagttttggc gtcgaaaagc tttgtgatga tatcgtggct
     9901 tgcatgacaa aatcccagtc ggcttccagg tgagaatatc tttgaaaaac tgttggcgta
     9961 tacggtatgg ccagtcttgt cgaaacactt gatgggcggg agaggttctc cactataccg
    10021 caactcctgg tatggatcgt cttcaaggat gataacatca tacttgctgc ctatttcagc
    10081 cagctttttc cgacgttcaa gagggaaagt cttgccagtt gggttttgga aggtgggaat
    10141 gatatataca agtttgggat gcagcttttg tattttcttt tctacatctt cgagtatcat
    10201 gccacagtcg tcacactcaa caccgacgca cctagcctgg aacatctcga agatctccac
    10261 acagtggaca aaggtgggcg attccaccag aataacgtca ccggggttga tatagacttg
    10321 acagagcagg ttcatggttt caagaccacc gttaacgatg atgatctcgt caagctctgc
    10381 ctccagcccc ttaggtttga gcagaatgtc tgcgacgttt tttctgaggt cggatacgcc
    10441 gagaacggag ccatattgta acgcctccac gccgcgtttg ttgcgggtga taacatcagt
    10501 acagatgtcg tgaactgctt ctactggcag cgcatcgctt gcaggggcgc cgccgccgag
    10561 agatataatg ttaggggaat ccatcgcgcc gaacagtgcg cgaatgattt tggcagagct
    10621 ttccatgtca tatattctct gtgcaaattt cggcatagcg tttcctcctt ctttaaagtt
    10681 atctccaccg atgaa
//
